• Tissue Engineering and Regenerative Medicine
• /
• 제15권6호
• /
• pp.771-779
• /
• 2018

BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent stem cells that can differentiate into several cell types. In addition, many studies have shown that MSCs modulate the immune response. However, little information is currently available regarding the maintenance of immunomodulatory characteristics of MSCs through passages. Therefore, we investigated and compared cytokine and gene expression levels from adipose (AD) and bone marrow (BM)-derived MSCs relevant to immune modulation from early to late passages. METHODS: MSC immunophenotype, growth characteristics, cytokine expressions, and gene expressions were analyzed. RESULTS: AD-MSCs and BM-MSCs had similar cell morphologies and surface marker expressions from passage 4 to passage 10. Cytokines secreted by AD-MSCs and BM-MSCs were similar from early to late passages. AD-MSCs and BM-MSCs showed similar immunomodulatory properties in terms of cytokine secretion levels. However, the gene expressions of tumor necrosis factor-stimulated gene (TSG)-6 and human leukocyte antigen (HLA)-G were decreased and gene expressions of galectin-1 and -3 were increased in both AD- and BM-MSCs with repeated passages. CONCLUSION: Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different.

• 대한한방부인과학회지
• /
• 제34권4호
• /
• pp.30-45
• /
• 2021

Objectives: This study was performed to investigate the effects of pregnancy-related four herbal medicines (Samul-tang, Onkyung-tang, Chokyungjongok-tang and Moutan Radicis Cortex) on the endometrial and placental cells. Methods: In this study, we examined viability and decidualization of telomerase immortalized human endometrial stromal cell lines (T-HESCs) and viability and invasion ability of human first trimester trophoblast cell lines Sw.71 by four herbal medicines (Samul-tang, Onkyung-tang, Chokyungjongok-tang and Moutan Radicis Cortex) Results: In the study, we showed that Samul-tang, Onkyung-tang, Chokyungjongok-tang increased decidualization marker prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1) in T-HESCs. Moutan Radicis Cortex decreased the mRNA level of PRL and IGFBP1, and the protein level of PRL and IGFBP1 had no significant effect. Moreover, four herbal medicines reduced invasion ability of Sw.71 cells. Conclusions: These results suggest that Samul-tang, Onkyung-tang, and Chokyungjongok-tang have beneficial effects on successful embryo implantation and pregnancy maintenance by increasing decidualization markers such as PRL and IGFBP1. Moutan Radicis Cortex reduces the mRNA levels of PRL and IGFBP1, which may adversely affect pregnancy. Further investigations are needed to elucidate the significance of the decreased invasive ability of Sw.71 cells induced by four herbal medications.

• 대한한방부인과학회지
• /
• 제34권4호
• /
• pp.111-130
• /
• 2021

Objectives: The purpose of this study was to identify the current status of Korean medicine clinical practice for pregnant patients. Methods: The study is conducted by searching medical records of 209 pregnant patients using Korean Standard Classification of Diseases related pregnancy at the Korean Gynecology Clinic of traditional Korean medicine hospital from March 1, 2016 to February 28, 2021. We retrospectively investigated characteristics, symptoms, pregnancy outcomes of the patients and treatments for the patients. Results: The most frequent symptoms of pregnant patients were musculoskeletal symptoms (60.63%) followed by neuropsychiatric symptoms (14.55%) and genital symptoms (10.82%). The most common contributory factor of the symptoms was the motor vehicle accident (74.64%). Acupuncture (94.94%) and moxibustion (90.91%) were performed to most pregnant patients. Cupping (86.60%), herbal medicine (52.63%), Chuna therapy (30.62%), and pharmacopuncture (21.05%) were performed to the patients. Normal pregnancy maintenance and delivery were reported by 147 patients (91.30%). Premature birth was reported by 11 patients (6.83%), and miscarriage was reported by 3 patients (1.86%). Conclusions: Most patients were the patients with musculoskeletal symptoms injured by motor vehicle accidents. Various Korean medicine treatments were performed during pregnancy with safety outcomes. This study could be used as basic data to provide instructions for the development of traditional Korean medicine in the obstetric clinical fields.

• Design & Manufacturing
• /
• 제14권4호
• /
• pp.17-24
• /
• 2020

Products for heating indoors in low temperature and dry winter are largely divided into products using fossil fuels and products using electricity. The fossil fuels can warm the entire space by convection, but there is a high risk of fire and the frequent ventilation due to the increase in carbon monoxide and carbon dioxide. Heaters using electricity are mainly used because they are convenient to use and are cheap. However, these products can not efficiently warm the air because they use radiation energy. In other words, only the front part exposed to the heater is warm, and the rear part has no heating effect at all. Also, because it emits a large amount of light, fatigue of the eyes is very high. Another problem is that when using electric heaters, the room tends to be dry by high heat. Indoor humidity maintenance is a very important factor in the prevention and treatment of respiratory diseases. Especially, it is essential for health care for infants, bronchial organs and people with weak respiratory because humidity is low in winter. In this study, we conducted a study to develop a product that can improve heating efficiency while maintaining proper indoor humidity by combining heat energy and moisture particles. The concept of humidification and heating at the same time, moisture particles generated in the humidifier pass through the heater, include thermal energy, and the moisture particles with thermal energy are diffused into the space by forced convection, thereby warming the entire space. In addition, the heating time is shortened as the feeling temperature is increased with the high relative humidity, and this has the effect that the heating cost in winter is reduced.

• 한국프랜차이즈경영연구
• /
• 제12권1호
• /
• pp.35-44
• /
• 2021

Purpose: This study examines the opportunism moderating effect by the startup experience in the relationship between franchisor and franchisees. In the case of a franchise system that has a continuous relational exchange transaction, relationship management is a very important activity because the relationship management between franchisor and franchisees improves the quality of the relationship. Nevertheless, there is insufficient of research on opportunism, which is a negative factor in managing the relationship between franchisor and franchisees in continuous relationship. Research design, data and methodology: This study, we explore the cause of opportunism based on transaction cost theory through prior research and establish a research model based by goal incongruity, uncertainty, information asymmetry, transaction specific assets, the relevance to determinant of opportunism and the startup experienced which is a moderating variable. To verify several hypotheses, the data were collected from 300 out of 1,760 domestic franchisees and analyzed using multiple regression analysis with SPSS program. Results: The findings are as follows. Goal incongruity did not affect opportunism. Opportunism increased as uncertainty increased, and as information asymmetry increased, opportunism increased. An opportunism decreased as transaction specific assets increased. Moreover, the findings show that startup experience only plays a moderating role in the relationship between information asymmetry and opportunism. Therefore, 4 out of 8 hypotheses were supported. Conclusions: The findings show that uncertainty, information asymmetry, and transaction specific assets are the determinants of opportunism. In addition, the results of the analysis of the moderating role of startup experience show that the less entrepreneurial experience, the greater the influence of information asymmetry on opportunism. Our findings mean that maintaining a successful relationship between franchisors and franchisees is possible when franchisors provide knowledge sharing, goal sharing, environmental sharing, and management information sharing to franchisees. In addition, the findings of this study shows that the contract content and management should be changed according to the entrepreneurial experience. In other words, the franchisors must share and integrate the accumulated franchisees' and franchisors' experience with the franchisees to create a synergy that can lead to successful bilateral relationship maintenance, which in turn reduces opportunism.

• 한국건설관리학회논문집
• /
• 제23권1호
• /
• pp.54-63
• /
• 2022

본 연구는 대형 국책사업의 성공요인을 도출하기 위해 용산국가공원 조성사업에 참여하고 있는 전문가를 대상으로 설문을 조사하여 프로그램 정의단계 핵심성공요인을 분석하였다. 정의단계 용산공원 조성사업의 참여하고 있는 전문가를 대상으로 한 설문조사를 통해 각각의 범주에 대해 AHP분석을 수행하여 상대적 중요도를 파악하였다. 대형 국책사업 프로그램 정의단계 중요한 범주는 프로그램 전략, 프로세스, 사람들로 분류되었으며 범주간 중요도는 사람들, 전략, 프로세스 순으로 나타났다. 또한, 프로그램 정의단계 27개 핵심성공요인들 중에서 프로그램관리자의 역량과 리더십, 환경 및 문화재 조사 기획, 프로그램 관리 계획 준비, 운영 및 유지 철학, 통합된 프로그램 사무소 등이 우선순위가 높게 나타났다. 따라서 용산공원 조성사업을 추진함에 있어서 본 연구에서 제시한 프로그램 정의단계 중요도가 높은 핵심성공요인에 대한 선택과 집중 전략을 제안한다.

• 한국지반신소재학회논문집
• /
• 제20권4호
• /
• pp.151-163
• /
• 2021

본 논문에서는 초속경 복합매트를 이용하여 비탈면 보강 시 비탈면 안정해석에 대한 내용을 다루었다. 최근 지구온난화에 따른 기상이변으로 태풍, 집중호우 등에 의해 철도 성토 노반 비탈면 유실로 열차 운행이 제한되고 있다. 또한 복구 비용보다 피해액이 증가하고 있으며 유실로 인한 작업환경 저하로 작업자들의 안전이 우려되고 있다. 이를 위해 기존에 다양한 비탈면 보호공법이 개발되었지만 철도 현장 특성상 중장비의 투입이 곤란하여 적용이 곤란한 실정이다. 따라서 본 연구에서는 시공기면 확보가 용이하여 시공성 및 작업 안정성을 증대시키며 집중호우 시 재유실 방지 및 식생생장을 원천적으로 차단하여 유지보수 비용을 절감할 수 있는 초속경 복합매트를 적용한 비탈면 안정해석을 실시하였다. 해석 결과 초속경 복합매트의 적용시 안전율 증가가 확인되었지만 초속경 복합매트의 두께에 따른 안전율 증가는 미미한 것으로 확인되었다.

• 대한간호학회지
• /
• 제52권5호
• /
• pp.522-534
• /
• 2022

Purpose: This study examined the validity and reliability of the Korean version of the Self-Care in Chronic Obstructive Pulmonary Disease Inventory (SC-COPDI) and the Chronic Obstructive Pulmonary Disease Self-Care Self-Efficacy Scale (SCES-COPD). The SC-COPDI consists of the Self-Care Maintenance Scale (SCMES), Self-Care Monitoring Scale (SCMOS), and Self-Care Management Scale (SCMAS). Methods: The original tool was translated using a back-translation process. Participants were 241 patients with COPD at the Chonnam National University Hospital in Korea. The construct validity was verified through confirmatory factor analysis, and reliability was verified using Cronbach's α. Results: The SCMES consisted of 10 items of three factors-one of four factors was deleted from the original tool. In the SCMOS, there were six items of two factors after two items were deleted from the original tool. The SCMAS consisted of the original 10 items of three factors. The SCES-COPD consisted of six items of two factors, with one item removed from the original tool. The model fit indices of all tools were good, and the construct validity was confirmed. Cronbach's α of SCMES was .72, SCMOS was .90, SCMAS was .81, and SCES-COPD was .85. Conclusion: The Korean version of SC-COPDI and SCES-COPD are valid and reliable instruments for measuring self-care in people with COPD. These instruments can be used in self-care studies of COPD patients in Korea.

• BMB Reports
• /
• 제55권8호
• /
• pp.370-379
• /
• 2022

Human pregnancy is a delicate and complex process where multiorgan interactions between two independent systems, the mother, and her fetus, maintain pregnancy. Intercellular interactions that can define homeostasis at the various cellular level between the two systems allow uninterrupted fetal growth and development until delivery. Interactions are needed for tissue remodeling during pregnancy at both fetal and maternal tissue layers. One of the mechanisms that help tissue remodeling is via cellular transitions where epithelial cells undergo a cyclic transition from epithelial to mesenchymal (EMT) and back from mesenchymal to epithelial (MET). Two major pregnancy-associated tissue systems that use EMT, and MET are the fetal membrane (amniochorion) amnion epithelial layer and cervical epithelial cells and will be reviewed here. EMT is often associated with localized inflammation, and it is a well-balanced process to facilitate tissue remodeling. Cyclic transition processes are important because a terminal state or the static state of EMT can cause accumulation of proinflammatory mesenchymal cells in the matrix regions of these tissues and increase localized inflammation that can cause tissue damage. Interactions that determine homeostasis are often controlled by both endocrine and paracrine mediators. Pregnancy maintenance hormone progesterone and its receptors are critical for maintaining the balance between EMT and MET. Increased intrauterine oxidative stress at term can force a static (terminal) EMT and increase inflammation that are physiologic processes that destabilize homeostasis that maintain pregnancy to promote labor and delivery of the fetus. However, conditions that can produce an untimely increase in EMT and inflammation can be pathologic. These tissue damages are often associated with adverse pregnancy complications such as preterm prelabor rupture of the membranes (pPROM) and spontaneous preterm birth (PTB). Therefore, an understanding of the biomolecular processes that maintain cyclic EMT-MET is critical to reducing the risk of pPROM and PTB. Extracellular vesicles (exosomes of 40-160 nm) that can carry various cargo are involved in cellular transitions as paracrine mediators. Exosomes can carry a variety of biomolecules as cargo. Studies specifically using exosomes from cells undergone EMT can carry a pro-inflammatory cargo and in a paracrine fashion can modify the neighboring tissue environment to cause enhancement of uterine inflammation.

• 대한한방부인과학회지
• /
• 제34권3호
• /
• pp.1-14
• /
• 2021

Methods: We investigated the ability to induce decidualization of human endometrial stromal cells and invasive ability of human trophoblast cells by water extract of three types herbal medicines (Dangguijakyak-san, Siryung-tang, Antae-eum) and ethanol extract on Scutellariae Radix. Results: In the process of decidualization of endometrial stromal cells, three herbal medicines including Dangguijakyak-san, Siryung-tang, and Scutellariae Radix increased the production of decidual markers such as prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1). However, Antae-eum increased mRNA levels of PRL and IGFBP and secretion of IGFBP in decidual stromal cells, but not PRL. Four herbal medicines inhibited the invasion of trophoblast cells. Conclusions: Four herbal medicines may play a role in implantation in women with reproductive failures. However, further studies are warranted to elucidate whether these medications are helpful in the maintenance of pregnancy.