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Effect of Tex1/THOC3, a component of THO complex, on growth and mRNA export in fission yeast (분열효모에서 THO 복합체의 구성요소인 Tex1/THOC3가 생장 및 mRNA 방출에 미치는 영향)

  • Bae, Soo Jeong;Koh, Eun-Jin;Yoon, Jin Ho
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.292-296
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    • 2017
  • In eukaryote, THO/TREX complex plays a critical role in transcriptional elongation, pre-mRNA processing, and nuclear mRNA export. This complex is evolutionally well- conserved, but there are some differences in composition and function according to organisms. Here we showed that spTex1, a component of THO/TREX complex, is not essential for growth and mRNA export in a fission yeast, Schizosaccharomyces pombe, which is more similar to higher eukaryote than budding yeast. Deletion and overexpression of the spTex1 gene do not lead to any detectable growth phenotype and accumulation of poly(A)+ RNA in the nucleus. And the spTex1-GFP protein is localized mainly in the nucleus. Yeast two-hybrid and Co-immunoprecipitation analysis showed that the spTex1 protein interacted with spHpr1 (THOC1) and spTho2 (THOC2), main subunits of THO complex. We conclude that the S. pombe Tex1 is a component of THO/TREX complex, but does not plays important roles in growth and bulk mRNA export from the nucleus.

Implementation of a Mixing-Ratio Control System for Two-Component Liquid Silicone Mixture (이액형 액상실리콘 재료의 혼합비율 제어 시스템 개발)

  • Choo, Seong-Min;Kim, Young-Min;Lee, Keum-Won
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.688-694
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    • 2018
  • The mixture ratio of two-component liquid silicone is important for the inherent physical characteristics of the finished product. Therefore, it is necessary to uniformly control the ratio of the main material and the sub-material. In this paper, a mixing-ratio control system was designed, which consists of a digital flow meter and a flow control system to measure the flow rate of the raw materials and a pumping system to maintain constant pressure and transfer of the raw materials. In addition, a program was developed to control the organic interlocking and mixing ratio. For the verification of the developed system, we compared the actual weight of raw material with the value measured by the flow meter during pumping, and we measured the physical properties of the mixed material by making test samples with and without the application of the mixing-ratio improvement algorithm. The measured value was close to the reference value with a hardness range of 46-47 and tensile strength of 9.3-9.5 MPa. These results show that the mixing ratio of the liquid silicone is controlled within an error range of ${\pm}0.5%$.

Analysis on Components of Conflict between Mentor Teachers and Mentee Teachers in Collaborative Mentoring for Beginning Science Teacher (초임 과학교사를 위한 협력적 멘토링 과정에서 나타나는 멘토교사와 멘티교사의 갈등 요소 분석)

  • Jung, Dojun;Lee, Imil;Nam, Jeonghee
    • Journal of Science Education
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    • v.43 no.1
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    • pp.119-135
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    • 2019
  • The purpose of this study is to analyze components of conflicts and investigate causes of them between mentor teachers and mentee teachers, who participated in the collaborative mentoring program to improve beginning science teachers teaching practice. The most often occurring component of conflict for surveyed teams was class strategy on knowledge of teaching methods. Then, conflicts in understanding student characteristics as a component of understanding students and knowledge transfer of concepts in science as a component of teaching contents followed as main causes. Changes in conflicts showed that less frequency of conflict occurred with ongoing mentoring. Conflicts from class strategy decreased the most, which had been the most often occurring cause. This decrease was explained as mentees were changed gradually into the teacher's role recognition through continuous mentoring. This study suggests that education for mentor and mentee teachers be provided with consideration of causes of conflicts and reasons for those causes, which eventually lead sustainable and efficient mentoring program management.

Modern Paradigm of Organization of the Management Mechanism by Innovative Development in Higher Education Institutions

  • Kubitsky, Serhii;Domina, Viktoriia;Mykhalchenko, Nataliia;Terenko, Olena;Mironets, Liudmyla;Kanishevska, Lyubov;Marszałek, Lidia
    • International Journal of Computer Science & Network Security
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    • v.22 no.11
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    • pp.141-148
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    • 2022
  • The development of the education system and the labor market today requires new conditions for unification and functioning, the introduction of an innovative culture in the field of Education. The construction of modern management of innovative development of a higher education institution requires consideration of the existing theoretical, methodological and practical planes on which its formation is based. The purpose of the article is to substantiate the modern paradigm of organizing the mechanism of managing the innovative development of higher education institutions. Innovation in education is represented not only by the final product of applying novelty in educational and managerial processes in order to qualitatively improve the subject and objects of management and obtain economic, social, scientific, technical, environmental and other effects, but also by the procedure for their constant updating. The classification of innovations in education is presented. Despite the positive developments in the development of Education, numerous problems remain in this area, which is discussed in the article. The concept of innovative development of higher education institutions is described, which defines the prerequisites, goals, principles, tasks and mechanisms of university development for a long-term period and should be based on the following principles: scientific, flexible, efficient and comprehensive. The role of the motivational component of the mechanism of innovative development of higher education institutions is clarified, which allows at the strategic level to create an innovative culture and motivation of innovative activity of each individual, to make a choice of rational directions for solving problems, at the tactical level - to form motives for innovative activity in the most effective directions, at the operational level - to monitor the formation of a system of motives and incentives, to adjust the directions of motivation. The necessity of the functional component of the mechanism, which consists in determining a set of steps and management decisions aimed at achieving certain goals of innovative development of higher education institutions, is proved. The monitoring component of the mechanism is aimed at developing a special system for collecting, processing, storing and distributing information about the stages of development of higher education institutions, prediction based on the objective data on the dynamics and main trends of its development, and elaboration of recommendations.

Synthesis and Properties of Coating Agents for Automobile Parts Using Water-born Polyurethane, Polysiloxane, and UHMWPP Powder (수분산 폴리우레탄, 폴리실록산, UHMWPP 분말을 이용하여 제조한 자동차 부품용 코팅제 합성 및 물성 연구)

  • Yong-Sung Kim;Sung-Jin Park;Young-hwan Kim;Hyejin Kim;Choong-Sun Lim;Bong-kuk Seo
    • Journal of Adhesion and Interface
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    • v.24 no.4
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    • pp.124-130
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    • 2023
  • For automotive coating agents that require little change in characteristics due to changes in external temperature, changes in noise characteristics due to changes in the external environment are very important. Therefore, soft polyurethane with excellent cold resistance, weather resistance, and abrasion resistance, and polysiloxane whose -OH terminated and side chains are modified with amines, are widely used. In this study, coating agents was prepared by adding water-born polyurethane, polysiloxane, ultra high molecular weight polypropylene (UHMWPP) powder, carbon black, and a matting agent to determine the effect of each resin component on noise. To study the effect of each resin component on noise, a coating agent was prepared by adding water-born polyurethane, polysiloxane, UHMWPP powder, carbon black, and a matting agent. The hard/soft segment ratio of water-born polyurethane, the main component of the coating, was 27.1%/72.9%, and the ratio of amino siloxanes to hydroxy-terminated polysiloxane was 2:7, which produced the least noise. The difference in friction coefficient was large when the friction body moves at high speed. When UHMWPP powder replaced SiO2, noise decreased and gloss also decreased.

Study on the Constituents of Angelica gigas root cultivated by Short-time Growing seedling (단기 육묘재배 당귀근의 부위별 성분연구)

  • 안상득
    • Korean Journal of Plant Resources
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    • v.9 no.2
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    • pp.197-201
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    • 1996
  • To greatly decrease bolting rate and deterioration of root quality, seeds of Angelica gigas had sown and grown several month earlier than traditional seedtime. Roots were harvested in the middle of November in same year. Roots were devided to several parts such a fune root, secondary root, stele and cortex of main root. We ditermined and compared with the major constisuents contained in each parts of 1 and 2 year old roots. Among each parts of root, the amount of constituents in the fine root and cortex were much higher than those in stele of main root. Content of decursin and decursinol angelate was higher 6.5% in 2 year old root than 4.8% in 1 year old root and their component ratio showed 10:7.5 in 1 year old root and 10:6.0 in 2 year old root, respectively. But merits as a material of oriental medicine for 1 year old root that cultivated by early short-time growing seedling were generally acknowledged.

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Excited States of Photoreceptor Molecules (I). Peridinin (광합성 에너지 수용색소 분자의 Excited States (第1報) Peridinin)

  • Pill-Soon Song;Tae Young Lee
    • Journal of the Korean Chemical Society
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    • v.23 no.5
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    • pp.314-319
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    • 1979
  • The electronic spectrum of marine dinoflagellate antenna pigment, peridinin, has been described in terms of PPP SCF MO CI computations and fluorescence polarization of the peridinin component in photosynthetic pigment complex of Amphidinium carterae. The main absorption band at 470 nm, $^1B{\leftarrow}A$, is polarized nearly along the long molecular axis. There appear to be two ${\pi}{\rightarrow}{\pi}^*$ transitions (C and $D{\leftarrow}A$) in the socalled "cis" peak region, and they are polarized roughly parallel to the main $B{\leftarrow}A$ absorption. In addition, we have found that the carbonyl group undergoes very 1ittle reorganization of the electronic structure in going from the ground to the $^1B$ excited states of peridinin, while the allenic group shows a strong charge transfer tendency in producing an electron-deficient allente allene group in the excited state.

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Cross-sectional Design and Stiffness Measurements of Composite Rotor Blade for Multipurpose Unmanned Helicopter (다목적 무인헬기 복합재 로터 블레이드의 단면 구조설계 및 강성 측정)

  • Kee, Young-Jung;Kim, Deog-Kwan;Shin, Jin-Wook
    • Journal of Aerospace System Engineering
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    • v.13 no.6
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    • pp.52-59
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    • 2019
  • The rotor blade is a key component that generates the lift, thrust, and control forces required for helicopter flight by the torque transmitted through the hub and the blade pitch angle control, and should be designed to factor vibration characteristics so that there is no risk of resonance with structural safety. In this study, the structural design of the main rotor blade for MPUH(Multi-Purpose Unmanned Helicopter) was conducted and the sectional stiffness measurement of the fabricated blade was performed. The evaluation of the vibration characteristics of the main rotor system was then conducted factoring the measured stiffness distribution. The interior of the rotor blade comprised of the skin, spar, and torsion box, and carbon and glass fiber composites were applied. The Ksec2D program was applied to predict the stiffness of blade, and the results were compared to the measured data. CAMRADII, a comprehensive rotorcraft analysis program, was applied to investigate the natural frequency trends and resonance risks due to the rotor rotation.

Near-infrared Extinction due to Cool Supernova Dust in Cassiopeia A

  • Lee, Yong-Hyun;Koo, Bon-Chul;Moon, Dae-Sik;Lee, Jae-Joon
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.35.1-35.1
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    • 2015
  • We present the results of extinction measurements toward the main ejecta shell of the Cassiopeia A supernova (SN) remnant using the flux ratios between the two near-infrared (NIR) [Fe II] lines at 1.26 and $1.64{\mu}m$. We find a clear correlation between the NIR extinction (E(J-H)) and the radial velocity of ejecta knots, showing that redshifted knots are systematically more obscured than blueshifted ones. This internal "self-extinction" strongly indicates that a large amount of SN dust resides inside and around the main ejecta shell. At one location in the southern part of the shell, we measure E(J-H) by the SN dust of $0.23{\pm}0.05mag$. By analyzing the spectral energy distribution of thermal dust emission at that location, we show that there are warm (~100K) and cool (~40K) SN dust components and that the latter is responsible for the observed E(J-H). We investigate the possible grain species and size of each component and find that the warm SN dust needs to be silicate grains such as $MgSiO_3$, $Mg_2SiO_4$, and $SiO_2$, whereas the cool dust could be either small (${\leq}0.01{\mu}m$) Fe or large (${\geq}0.01{\mu}m$) Si grains. We suggest that the warm and cool dust components in Cassiopeia A represent grain species produced in diffuse SN ejecta and in dense ejecta clumps, respectively.

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Design of a Reducer Gear for Small Electric Vehicles (소형 전기자동차용 감속기 설계)

  • Lee, Jae-gu;Kim, Sung-Hoon;Han, Sung-Gil;Shin, Yoo-In;Song, Chul Ki
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.19 no.9
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    • pp.116-121
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    • 2020
  • In recent times, fuel economy enhancement and environmental regulation compliance have become the main topics of interest in the automobile industry. Electric vehicles are desirable alternatives to the existing cars that employ internal combustion engines. Specifically, electric vehicles are equipped with inverters, motors, and a gearbox instead of engines and transmission mechanisms. The gearbox is a key component, used to transmit power from the electric motor to the wheel. Therefore, the design of the gearbox is critical. However, most engineers design gears based only on their experience because no standards pertaining to the design factor exist, other than those for the gear ratios. To overcome this problem, the structural stabilities must be examined considering the design factors of the gears. In this study, we considered the module and number of teeth as the main factors. The constraints corresponded to the final gear ratio and fixed distance between each axle of the shafts. Moreover, a structural analysis was conducted, and the variation trend of the maximum equivalent stress against changes in the gear module and number of teeth was examined. By performing such an analysis, the structural stability in the design of a gear system could be effectively investigated.