• Parasites, Hosts and Diseases
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• 제41권1호
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• pp.27-33
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• 2003

The developmental features, growth and organogenesis of Macroorchis spinuiosus were observed in albino rats. Globular and thick walled metacercariae, possessed a stylet, Y-shaped excretory bladder and extracecal testes. Un albino rats, M. spinulosus showed habitat shifting. The majority of M. spinulosus reside in the jejunum for the first four days post Infection (p.i.) and migrate to the duodenum at the later stage of infection. M. spinulosus grew rapidly during the first four days and reached full maturity at 14 days p.i. and later reduced in size. The ovary was separated from the genital primodium at one day p.i. The seminal vesicle appeared on the third day and divided into two sacs on the fourth day p.i. and intrauterine eggs and sperm mass were produced on the fourth day. Organogenesis and enlargement of reproductive organs governed the growth of M. spinulosus. The similarity of related species of the genus Macroorchis to M. spinulosus was discussed In consideration to developmental features.

• Parasites, Hosts and Diseases
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• 제42권4호
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• pp.151-157
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• 2004

Developmental surface ultrastructure of Macroorchis spinulosus was studied by scanning electron microscopy. One-day-old juvenile fluke was leaf-shaped and bent ventrally. Body surface was covered densely with peg-like spines and with cobblestone-like cytoplasmic processes. Ciliated sensory papillae were concentrated around oral sucker. Several unciliated sensory papillae occurred equidistantly on oral sucker and acetabulum. The ciliated papillae appeared in two longitudinal lines symmetric bilaterally on dorsal surface. On adult flukes, tegumantal spines became wider in middle of the body surface. The cytoplasmic processes differentiated into more fine velvety form. It is likely that the differentiated fine cytoplasmic processes are an increased absorptive surface to adult M. spinulosus. It is suggested that single pointed tegumental spines on anterior half of the body may be supportive for this fluke to migration.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.109-112
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• 2016

We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae from crayfish in Jeollanam-do Province, Korea. The metacercariae were round, $180{\mu}m$ in average diameter, encysted with 2 layers of thick walls, but the stylet on the oral sucker was not clearly seen. The adult flukes were oval shape, and $760-820{\mu}m$ long and $320-450{\mu}m$ wide, with anterolateral location of 2 large testes. The phylogenetic tree based on ITS1 sequences of 6 M. spinulosus samples showed their distinguished position from other trematode species in GenBank. The most closely resembled group was Paragonimus spp. which also take crayfish or crabs as the second intermediate host. The present study is the first molecular characterization of M. spinulosus and provided a basis for further phylogenetic studies to compare with other trematode fauna in Korea.

• Parasites, Hosts and Diseases
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• 제34권1호
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• pp.1-6
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• 1996

강원도 양양군 현북면 어성전리에 서식하는 참가재(Cambaroines simiLis)의 근육에서 Marroorchis spinulosus Ando. 1918(Digenea: Nanophyetidae)의 피낭유충을 검출하고 마우스 흰쥐 및 고양이에 실험감염시켜 성충을 얻은 다음 형태학적 특징을 관찰하였다. 실험동물 들의 소장으로부터 감염 5-21일에 총 117마리의 충체를 회수하였으며. 평균 충체회수율은 31-37%로서 동물별로 큰 차이가 없었다. 피낭유충은 등글고. 평균 직경 0.18 mm이며. 2개의 두꺼운 막으로 둘러싸여 있고. Y-자형의 배걸낭을 가지고 있었다. 성충은 대부분 난형이며 길이 0.66-0 71 mm. 폭 0.36-0.41 mm이었다. 구흡반에는 침(stylets) 1개가 있고. 난소가 체중앙부 근처에서 다양하게 위치하며. 저정낭이 이낭형(bilobed)이고 두 고환이 약간 전측방에 위치하는 점 등의 형태학적 특성을 나타내었다. 실험 감염에서 얻은 성충들을 M. spinulosus로 동정할 수 있었으나 그 형태가 최초 기록과 몇 가지 다른 점이 있어 앞으로 이 흡충의 원환한 진단을 위왜 충체의 형태를 수정하여 재기록하였다 이 장흡충푸 국내에는 미 기록종이며 포유동물을 종숙주로 하고 있을 것으로 추측되며 인체 감염 가능성도 배제할 수 없다. 이 충체의 우리발 이름을 가시큰고환흡충으로 부를 것을 제안한다.