• 한국동물생명공학회지
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• 제37권3호
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• pp.169-175
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• 2022

Bovine mammary epithelial (MAC-T) cells are commonly used to study mammary gland development and mastitis. Lipopolysaccharide is a major bacterial cell membrane component that can induce inflammation. Autophagy is an important regulatory mechanism participating in the elimination of invading pathogens. In this study, we evaluated the mechanism underlying bacterial mastitis and mammary cell death following lipopolysaccharide treatment. After 24 h of 50 ㎍/mL lipopolysaccharide treatment, a significant decrease in the proliferation rate of MAC-T cells was observed. However, no changes were observed upon treatment of MAC-T cells with 10 ㎍/mL of lipopolysaccharide for up to 48 h. Thus, upon lipopolysaccharide treatment, MAC-T cells exhibit dose-dependent effects of growth inhibition at 10 ㎍/mL and death at 50 ㎍/mL. Treatment of MAC-T cells with 50 ㎍/mL lipopolysaccharide also induced the expression of autophagy-related genes ATG3, ATG5, ATG10, ATG12, MAP1LC3B, GABARAP-L2, and BECN1. The autophagy-related LC3A/B protein was also expressed in a dose-dependent manner upon lipopolysaccharide treatment. Based on these results, we suggest that a high dose of bacterial infection induces mammary epithelial cell death related to autophagy signals.

• Asian-Australasian Journal of Animal Sciences
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• 제30권6호
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• pp.878-885
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• 2017

Objective: Glucose is an essential fuel in the energy metabolism and synthesis pathways of all mammalian cells. In lactating animals, glucose is the major precursor for lactose and is a substrate for the synthesis of milk proteins and fat in mammary secretory (alveolar) epithelial cells. However, clear utilization of glucose in mammary cells during lactogenesis is still unknown, due to the lack of in vitro analyzing models. Therefore, the objective of this study was to test the reliability of the mammary alveolar (MAC-T) cell as an in vitro study model for glucose metabolism and lactating system. Methods: Undifferentiated MAC-T cells were cultured in three types of Dulbecco's modified Eagle's medium with varying levels of glucose (no-glucose: 0 g/L, low-glucose: 1 g/L, and high-glucose: 4.5 g/L) for 8 d, after which differentiation to casein secretion was induced. Cell proliferation and expression levels of apoptotic genes, Insulin like growth factor-1 (IGF1) receptor, oxytocin receptor, ${\alpha}S1$, ${\alpha}S2$, and ${\beta}$ casein genes were analyzed at 1, 2, 4, and 8 d after differentiation. Results: The proliferation of MAC-T cells with high-glucose treatment was seen to be significantly higher. Expression of apoptotic genes was not affected in any group. However, expression levels of the mammary development related gene (IGF1 receptor) and lactation related gene (oxytocin receptor) were significantly higher in the low-glucose group. Expressions of ${\alpha}S1-casein$, ${\alpha}S2-casein$, and ${\beta}-casein$ were also higher in the low-glucose treated group as compared to that in the no-glucose and high-glucose groups. Conclusion: The results demonstrated that although a high-glucose environment increases cell proliferation in MAC-T cells, a low-glucose treatment to MAC-T cells induces higher expression of casein genes. Our results suggest that the MAC-T cells may be used as an in vitro model to analyze mammary cell development and lactation connected with precise biological effects.

• 공업화학
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• 제30권4호
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• pp.472-478
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• 2019

막 축전식 탈염(MCDI) 셀에 공급하는 총 전하(TC)를 조절하여 경도물질($Ca^{2+}$)을 안정하게 탈염할 수 있는 방법을 연구하였다. 흡착과정에서 TC를 변화시키면서 흡착(1.5 V)과 탈착(0.0 V)을 30회 반복하였다. 탈염과정에서 유출수의 농도와 pH, 흡착 및 탈착량, 전류밀도와 셀 전위의 변화를 분석하였다. MCDI 셀에 사용된 탄소전극의 최대허용전하(MAC)는 46 C/g로 측정되었다. MAC 이하의 TC (40 C/g)에서 운전한 결과 전극반응이 일어나지 않아 장기간 운전에서도 안정적인 탈염특성을 얻을 수 있었다. 반면, MAC 이상의 TC (50, 60 C/g)에서 운전한 경우 유출수의 농도와 pH가 크게 변하였다. 또한 전극반응으로 인해 전극표면에 스케일이 생성되어 셀의 전기저항이 점차 증가하였다. 이를 통해 흡착과정에서 MCDI 셀에 공급하는 전하를 제어함으로써 전극반응 없이 경도물질을 안정하게 제거할 수 있음을 확인하였다.

• International Journal of Stem Cells
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• 제9권2호
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• pp.186-191
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• 2016

Background and Objectives: With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. Methods and Results: MAC-T cells ($1{\times}10^7$) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. Conclusions: These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.

• Journal of Animal Science and Technology
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• 제64권5호
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• pp.922-936
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• 2022

5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in other tissues have shown that 5-HT can effectively promote cell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows is still unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability of bovine mammary epithelial cells and its related signaling pathways, (2) interaction between prolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T) were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then were assayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. The results suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which was mainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increase caused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translation elongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-T cells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and the level of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. In conclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR 1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT.

• Journal of Animal Science and Technology
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• 제64권1호
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• pp.110-122
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• 2022

The reduction of milk yield caused by heat stress in summer is the main condition restricting the economic benefits of dairy farms. To examine the impact of hyperthermia on bovine mammary epithelial (MAC-T) cells, we incubated the MAC-T cells at thermal-neutral (37℃, CON group) and hyperthermic (42℃, HS group) temperatures for 6 h. Subsequently, the cell viability and apoptotic rate of MAC-T cells, apoptosis-related genes expression, casein and amino acid transporter genes, and the expression of the apoptosis-related proteins were examined. Compared with the CON group, hyperthermia significantly decreased the cell viability (p < 0.05) and elevated the apoptotic rate (p < 0.05) of MAC-T cells. Moreover, the expression of heat shock protein (HSP)70, HSP90B1, Bcl-2-associated X protein (BAX), Caspase-9, and Caspase-3 genes was upregulated (p < 0.05). The expression of HSP70 and BAX (pro-apoptotic) proteins was upregulated (p < 0.05) while that of B-cell lymphoma (BCL)2 (antiapoptotic) protein was downregulated (p < 0.05) by hyperthermia. Decreased mRNA expression of mechanistic target of rapamycin (mTOR) signaling pathway-related genes, amino acid transporter genes (SLC7A5, SLC38A3, SLC38A2, and SLC38A9), and casein genes (CSNS1, CSN2, and CSN3) was found in the heat stress (HS) group (p < 0.05) in contrast with the CON group. These findings illustrated that hyperthermia promoted cell apoptosis and reduced the transport of amino acids into cells, which inhibited the milk proteins synthesis in MAC-T cells.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(2)
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• pp.357-360
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• 2000

In this paper, we designed 32-Hit MAC architecture of a RISC Processor for portable terminals such as cellular telephones, personal digital assistants, notebooks, etc. In order to have minimum area with best performance, the MAC performs 32 by 8 multiplication per cycle, with early termination circuit that enables multiply cycles depend on the value of multiplier. It uses the sign bit of a partial product and two extra bits for sign extension, The MAC is modeled and simulated in RTL using VHDL. The MAC is synthesized using IDEC C-631 Cell library based on 0.6$\mu\textrm{m}$ CMOS 1-Poly 3-metal CMOS technology.

• 전자공학회논문지S
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• 제35S권1호
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• pp.1-13
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• 1998

The next generation of wireless network will be problably developed as a mobile communications which will support ATM-based multiMedium traffic. We need to develop an dffective MAC protocol in order to support multimedia traffic having variety of QoS characteristics on wireless neteworks. In this paper, we propose a MAC protocol a MAC protocol where mobile terminals having cells to transmit, request slots to base station through requested slot then the base station analyze parameters from mobile and allocate slots to mobile according to priority. The multimedia slot allocation(MSA) protocol divides multimedias applications into real-time/ variant and non real-time services. Entire slots of the frameare partitioned proportionallyby sizeof bandwidth according to types of services, so that related services can use allocated-slots in priority. The proposed algorithm guarantees real time operation since real-time service share slots allocated for non real-real services. The algorithm whih divides slots of the frame is called as an Algorithm A, otherwise as an Algorithm B. The simulation compares by average delay time and cell loss probability between Algorithm and Algorithm B by increasing number of mobile terminal using the proposed MAC protocol. the simulation result for real-time services shows that average delay time and cell loss probability of Algorithm A is better than those of Algorithm B.

• 한국정보통신학회논문지
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• 제4권1호
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• pp.3-15
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• 2000

ATM PON은 국사 내에서 하나의 OLT가 수동 (passive) 광 분기 방식을 통해 가입자 지역의 최대 64 ONU를 제어함으로써, FTTs 형태의 가입자를 하나의 플랫폼에서 경제적으로 수용할 수 있는 장점이 있다. 이 시스템이 동작하기 위해서는 효율적인 MAC 프로토콜이 구현되어야 하는데, ITU-T 0.983.1에서 MAC 분야는 아직 추후 연구 과제로 남아있다. 본 논문은 G.983.1의 ATM PON이 동작하기 위한 MAC 프로토콜에서 ONU가 상향으로 셀을 전송하는데 필요한 승인요청 방법을 제안한다. G.983.1 기반이 아닌 MAC 프로토콜의 장단점을 살펴본 후, 승인요청에 필요한 미니슬롯의 주기와 길이를 계산하고 구현에 적합한 최적 값을 결정한다. 또한, 물리계층 OAM 셀을 코딩하는 방법과 결정된 파라메터들을 할당하는 절차를 제안한다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2001년도 춘계종합학술대회
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• pp.125-128
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• 2001

광대역 액세스 망에서 합류, 분배 지점에서의 효율을 고려한 PON(Passive Optical Network)의 설계는 중요한 관심거리가 되고 있다. PON 구조는 매우 간단하지만, 가입자들에서 발생하는 상향 트래픽 제어를 위해서는 매체 접근 제어 프로토콜이 필요하다. 이러한 매체 접근 제이 프로토콜은 트래픽 클래스별 QoS(Duality of Service) 관리 기능, 효율적인 망 자원 분배 기능, CDV(Cell Delay Variation) 최소화 기능 등이 포함되어야 한다. 본 논문에서는 ATM-PON과 흠 네트워크 환경에 적합한 성능을 제공하고 앞서 기술한 기능을 수행하는 매체 접근 제이 프로토콜 설계에 관한 연구 내용을 기술한다. 설계된 프로토콜은 요구-할당(Request-Permit) 알고리즘을 사용하여 동적인 상향 대역 할당이 가능하게 설계하였다. 이를 위해 ITU-T 권고 G.983:1을 이용하였고, 이에 더하여 새로운 메시지 형식과 동적 대역할당 알고리즘 등을 제시하였다.