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Discrimination and Hordein Polypeptide Patterns of Malting Barley Varieties Using UPLC (UPLC 분석을 이용한 맥주보리 품종의 호데인 단백질 분석 및 품종 판별)

  • Yoon, Young-Mi;Kim, Yang-Kil;Kang, Chon-Sik;Park, Jin-Cheon;Park, Tae-Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.326-338
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    • 2021
  • Ultra-performance liquid chromatography (UPLC) was used to assess the hordein protein fraction of malting barley. C-hordeins (barley prolamins) were extracted with 70% ethanol (EtOH) and 55% isopropyl alcohol (IPA, 2-propanol), and B-hordeins were extracted with the same alcohols in 1.0% dithiothreitol (DTT). High molecular weight (HMW) prolamins (D-hordeins) were extracted with 50% IPA with 1M Tris-HCl (pH 8.0). The same protein patterns were observed in both the experimental extraction solutions (EtOH and IPA). However, the patterns of hordein, extracted with EtOH and IPA containing 1.0% DTT, differed slightly. C- and B-hordeins extracted from those solutions were analyzed. Twenty-six malting barley varieties developed in Korea were analyzed using UPLC. The varieties were divided into seven groups according to hordein patterns of retention time 16 min to 18 min, and 20 varieties showed unique patterns.

Prevalence of hepatitis E virus antibodies in cattle in Burkina Faso associated with swine mixed farming

  • Tialla, Dieudonne;Cisse, Assana;Ouedraogo, Georges Anicet;Hubschen, Judith M.;Tarnagda, Zekiba;Snoeck, Chantal J.
    • Journal of Veterinary Science
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    • v.23 no.3
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    • pp.33.1-33.10
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    • 2022
  • Background: Endemic circulation of human-specific hepatitis E virus (HEV) genotypes 1 and 2 may occult the importance of sporadic zoonotic HEV transmissions in Africa. Increasing numbers of studies reporting anti-HEV antibodies in cattle and the discovery of infectious HEV in cow milk has raised public health concern, but cattle exposure has seldom been investigated in Africa. Objectives: This study aimed at investigating the role of cows in the epidemiology of HEV in Burkina Faso and farmers habits in terms of dairy product consumption as a prerequisite to estimate the risk of transmission to humans. Methods: Sera from 475 cattle and 192 pigs were screened for the presence of anti-HEV antibodies while HEV RNA in swine stools was detected by reverse transcription polymerase chain reaction. Data on mixed farming, dairy product consumption and selling habits were gathered through questionnaires. Results: The overall seroprevalence in cattle was 5.1% and herd seroprevalence reached 32.4% (11/34). Herd seropositivity was not associated with husbandry practice or presence of rabbits on the farms. However, herd seropositivity was associated with on-site presence of pigs, 80.7% of which had anti-HEV antibodies. The majority of farmers reported to preferentially consume raw milk based dairy products. Conclusions: Concomitant presence of pigs on cattle farms constitutes a risk factor for HEV exposure of cattle. However, the risk of HEV infections associated with raw cow dairy product consumption is currently considered as low.

Molecular dynamics study of liquid sodium film evaporation and condensation by Lennard-Jones potential

  • Wang, Zetao;Guo, Kailun;Wang, Chenglong;Zhang, Dalin;Tian, Wenxi;Qiu, Suizheng;Su, Guanghui
    • Nuclear Engineering and Technology
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    • v.54 no.8
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    • pp.3117-3129
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    • 2022
  • Deeply understanding the phase change of thin liquid sodium film inside wick pore is very important for further studying high-temperature sodium heat pipe's heat transfer. For the first time, the evaporation and condensation of thin liquid sodium film are investigated by the Lennard-Jones potential of molecular dynamics. Based on the startup and normal operation of the sodium heat pipe, three different cases are simulated. First, the equilibrium is achieved and the Mass Accommodation Coefficients of the three cases are 0.3886, 0.2119, 0.2615 respectively. Secondly, the non-equilibrium is built. The change of liquid film thickness, the number of gas atoms, the net evaporation flux (Jnet), the heat transfer coefficient (h) at the liquid-gas interface are acquired. Results indicate that the magnitude of the Jnet and the h increase with the basic equilibrium temperature. In 520-600 K (the startup of the heat pipe), the h has approached 5-6 W m-2 K-1 while liquid film thickness is in 11-13 nm. The fact shows that during the initial startup of the sodium heat pipe, the thermal resistance at the liquid-gas interface can't be negligible. This work is the complement and extension for macroscopic investigation of heat transfer inside sodium heat pipe. It can provide a reference for further numerical simulation and optimal design of the sodium heat pipe in the future.

Characteristics of the Microbial Community Responding to the Vertical Distribution of TPH Concentrations in the Petroleum-Contaminated Site (유류오염지역 부지 내 TPH 수직 농도 분포에 따른 미생물 생태 특성)

  • Song, Soo Min;Moon, Hee Sun;Han, Ji Yeon;Shin, Jehyun;Jeong, Seung Ho;Jeong, Chan-Duck;Cho, Sunghyen
    • Journal of Soil and Groundwater Environment
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    • v.27 no.spc
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    • pp.51-63
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    • 2022
  • In this study, the TPH(Total Petroleum Hydrocarbon) contamination and microbial ecological characteristics in petroleum-contaminated site were investigated through the correlation among the vertical TPH contamination distribution of the site, the geochemical characteristics, and the indigenous microbial ecology. The high TPH concentration showed in the vicinity of 3~4 m or less which is thought to be affected by vertical movement due to the impervious clay layer. In addition, the TPH concentration was found to have a positive correlation with Fe2+, TOC concentration, and the number of petroleum-degrading bacteria, and a negative correlation with the microbial community diversity. The microbial community according to the vertical distribution of TPH showed that Proteobacteria and Firmicutes at the phylum level were dominant in this study area as a whole, and they competed with each other. In particular, it was confirmed that the difference in the microbial community was different due to the difference in the degree of vertical TPH contamination. In addition, the genera Acidovorax, Leptolinea, Rugoshibacter, and Smithella appeared dominant in the samples in which TPH was detected, which is considered to be the microorganisms involved in the degradation of TPH in this study area. It is expected that this study can be used as an important data to understand the contamination characteristics and biogeochemical and microbial characteristics of these TPH-contaminated sites.

Effect of LED LightIrradiation on the Mycelial Growth and Fruit Body Development of Hypsizygus Marmoreus (LED 광원이 느티만가닥버섯 균의 균사 생장과 자실체 생육에 미치는 영향)

  • Kim, M.K.;Lee, Y.K.;Seo, G.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.22 no.1
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    • pp.99-112
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    • 2020
  • A edible mushroom, Hypsizygus marmoreus is commercially cultivated. However, the researches of cultivation and physiological characteristics were not conducted in Korea. In this study, we conducted on artificial cultivation of H. marmoreus and elucidated the effect of light on the mycelial growth and fruit body development using LED light sources with different wavelength; blue (peak wave length 460nm), green(peak wave length 530nm), yellow(peak wave length 590nm), red(peak wave length 630nm), and white as positive control. Mycelial growth of H. marmoreus strains were inhibited about 30~40% in inhibition ratio under the illumination with blue, green, yellow LED light. However, red LED light was not inhibited. Elongation of stipe was effective under the long wave length such as yellow and red light. Abnormal fruit body was produced under the long wavelength and dark. However, development of pileus was effective under the short wavelength such as green and blue light. Also, as a result of cultivation with mixed light for high quality and harvest, many effective numbers and yields of fruiting bodies were obtained in the mixed treatment of blue and white light, and pileus developed well.

SOCS1 counteracts ROS-mediated survival signals and promotes apoptosis by modulating cell cycle to increase radiosensitivity of colorectal cancer cells

  • Ryu, Ji-Yoon;Oh, Jiyoung;Kim, Su-Min;Kim, Won-Gi;Jeong, Hana;Ahn, Shin-Ae;Kim, Seol-Hee;Jang, Ji-Young;Yoo, Byong Chul;Kim, Chul Woo;Lee, Choong-Eun
    • BMB Reports
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    • v.55 no.4
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    • pp.198-203
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    • 2022
  • As negative regulators of cytokine signaling pathways, suppressors of cytokine signaling (SOCS) proteins have been reported to possess both pro-tumor and anti-tumor functions. Our recent studies have demonstrated suppressive effects of SOCS1 on epithelial to mesenchymal signaling in colorectal cancer cells in response to fractionated ionizing radiation or oxidative stress. The objective of the present study was to determine the radiosensitizing action of SOCS1 as an anti-tumor mechanism in colorectal cancer cell model. In HCT116 cells exposed to ionizing radiation, SOCS1 over-expression shifted cell cycle arrest from G2/M to G1 and promoted radiation-induced apoptosis in a p53-dependent manner with down-regulation of cyclin B and up-regulation of p21. On the other hand, SOCS1 knock-down resulted in a reduced apoptosis with a decrease in G1 arrest. The regulatory action of SOCS1 on the radiation response was mediated by inhibition of radiation-induced Jak3/STAT3 and Erk activities, thereby blocking G1 to S transition. Radiation-induced early ROS signal was responsible for the activation of Jak3/Erk/STAT3 that led to cell survival response. Our data collectively indicate that SOCS1 can promote radiosensitivity of colorectal cancer cells by counteracting ROS-mediated survival signal, thereby blocking cell cycle progression from G1 to S. The resulting increase in G1 arrest with p53 activation then contributes to the promotion of apoptotic response upon radiation. Thus, induction of SOCS1 expression may increase therapeutic efficacy of radiation in tumors with low SOCS1 levels.

Characteristics and Restoration Strategies of Warm-Temperate Forests Vegetation Types in Island Area on the Korean Peninsula (한반도 도서지역의 난온대림 식생유형 특징 및 복원전략)

  • Kang, Hyun-Mi;Kang, Ji-Woo;Sung, Chan-Yong;Park, Seok-Gon
    • Korean Journal of Environment and Ecology
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    • v.36 no.5
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    • pp.507-524
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    • 2022
  • In this study, we revealed the location environment and community structural characteristics after extensively investigating Korea's warm-temperate island areas and categorizing vegetation through TWINSPAN analysis. Based on it, this study aims to suggest the direction of the vegetation restoration plan for warm-temperate forests by deriving a restoration strategy for each vegetation type. The vegetation types were clearly divided into eight types, and communities I through IV were good evergreen broad-leaved forests dominated by Machilus thunbergii and Castanopsis sieboldii. On the other hand, communities V through VIII were Pinus thunbergii forest, deciduous broad-leaved forest, and artificial forest, and retrogressive succession vegetation in the warm-temperate areas. The environmental factors derived from the DCA analysis were altitude (average temperature of the coldest month) and distance from the coastline (salt tolerance). The distribution pattern of warm-temperate forests has been categorized into M. thunbergii, C. sieboldii and Cyclobalanopsis spp. forest types according to the two environmental factors. It is reasonable to apply the three vegetation types as restoration target vegetation considering the location environment of the restoration target site. In communities V through VIII, P. thunbergiiand deciduous broad-leaved formed a canopy layer, and evergreen broad-leaved species with strong seed expansion frequently appeared in the ground layer, raising the possibility of vegetation succession as evergreen broad-leaved forests. The devastated land where forests have disappeared in the island areas is narrow, but vegetation such as P. thunbergii and deciduous broad-leaved forests, which have become a retrogressive succession, forms a large area. The restoration strategy of renewing this area into evergreen, broad-leaved forests should be more effective in realizing carbon neutrality and promoting biodiversity.

Structural and Electrical Properties of La0.7Sr0.3MnO3 Thin Films for Thermistor Applications (서미스터로의 응용을 위한 La0.7Sr0.3MnO3 박막의 구조적, 전기적 특성)

  • Lim, Jeong-Eun;Park, Byeong-Jun;Yi, Sam-Haeng;Lee, Myung-Gyu;Park, Joo-Seok;Kim, Byung-Cheul;Kim, Young-Gon;Lee, Sung-Gap
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.35 no.5
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    • pp.499-503
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    • 2022
  • La0.7Sr0.3MnO3 precursor solution were prepared by a sol-gel method. La0.7Sr0.3MnO3 thin films were fabricated by a spin-coating method on a Pt/Ti/SiO2/Si substrate. Structural and electrical properties with the variation of sintering temperature were measured. All specimens exhibited a polycrystalline orthorhombic crystal structure, and the average thickness of the specimens coated 6 times decreased from about 427 nm to 383 nm as the sintering temperature increased from 740℃ to 830℃. Electrical resistance decreased as the sintering temperature increased. In the La0.7Sr0.3MnO3 thin films sintered at 830℃, electrical resistivity, TCR, B-value, and activation energy were 0.0374 mΩ·cm, 0.316%/℃, 296 K and 0.023 eV, respectively.

A Performance Study on CPU-GPU Data Transfers of Unified Memory Device (통합메모리 장치에서 CPU-GPU 데이터 전송성능 연구)

  • Kwon, Oh-Kyoung;Gu, Gibeom
    • KIPS Transactions on Computer and Communication Systems
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    • v.11 no.5
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    • pp.133-138
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    • 2022
  • Recently, as GPU performance has improved in HPC and artificial intelligence, its use is becoming more common, but GPU programming is still a big obstacle in terms of productivity. In particular, due to the difficulty of managing host memory and GPU memory separately, research is being actively conducted in terms of convenience and performance, and various CPU-GPU memory transfer programming methods are suggested. Meanwhile, recently many SoC (System on a Chip) products such as Apple M1 and NVIDIA Tegra that bundle CPU, GPU, and integrated memory into one large silicon package are emerging. In this study, data between CPU and GPU devices are used in such an integrated memory device and performance-related research is conducted during transmission. It shows different characteristics from the existing environment in which the host memory and GPU memory in the CPU are separated. Here, we want to compare performance by CPU-GPU data transmission method in NVIDIA SoC chips, which are integrated memory devices, and NVIDIA SMX-based V100 GPU devices. For the experimental workload for performance comparison, a two-dimensional matrix transposition example frequently used in HPC applications was used. We analyzed the following performance factors: the difference in GPU kernel performance according to the CPU-GPU memory transfer method for each GPU device, the transfer performance difference between page-locked memory and pageable memory, overall performance comparison, and performance comparison by workload size. Through this experiment, it was confirmed that the NVIDIA Xavier can maximize the benefits of integrated memory in the SoC chip by supporting I/O cache consistency.

Breeding of a new cultivar of White Flammulina velutipes, 'Seolhan' (흰색 팽이 신품종 '설한' 육성)

  • Im, Ji-Hoon;Oh, Minji;Oh, Youn-Lee;Kim, Minseek;Lee, Yun-Sang
    • Journal of Mushroom
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    • v.19 no.4
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    • pp.279-284
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    • 2021
  • Flammulina velutipes is the representative edible mushroom in Korea. It makes up around 18% of the total mushroom production and accounts for about 36% of the total mushroom export. Despite being one of the most industrially important mushroom in Korea, the distribution rate of domestic cultivar was low (~31.6%) in 2020. Therefore, we need a new domestic cultivar with high quality as the main export mushroom. We developed a new white domestic cultivar, 'Seolhan', bred by mating two monokaryotic strains isolated from Baekseung and Woori-1ho. The optimum mycelial growth temperature was 25℃ and Seolhan had higher mycelial growth than the control at 20 and 25℃. In bottle cultivation, the optimum temperature of incubation was from 15℃ to 17℃ and the growth temperature of Seolhan was 7-8℃, which was slightly higher than that of the control. The yield of Seolan was 226.5±8.0 g, an increase of 18.4% compared to the control. The period of primodia formation also was a couple of days ahead. In morphological traits, the pileus of Seolhan was smaller than that of the control, the stipe was thicker, and the color brighter. As the results of replacement culture and genetic diversity test, it was confirmed that Seolhan was genetically different from other white varieties.