• Korean Journal of Medicinal Crop Science
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• v.18 no.3
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• pp.143-150
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• 2010

This study showed the increase of antitumor activities of water soluble E. sinica extract by nano-encapsulation process with lecithin. Five groups of lecithin only group (LO), lecithin nano-encapsulated E. sinica group (LE), E. sinica only group (EO), one negative control group (NCO) and positive control group (PCO) were set for several anticancer experiment and fed into Sarcoma-180 injected mice. The cytotoxicity of LE on the human normal kidney cell (HEK293) showed 14.8% lower than 19.2% of EO and 18.4% of LO. Growth of human liver carcinoma cell and human stomach carcinoma cell as representative of digestive system in vitro was inhibited up to about 85.1% and 87.3%, in adding 1.0 mg/$m{\ell}$ of LE, which values 15% higher than that from conventional EO. The survival rates of each mice group were 40%, 63%, 48%, 33% and 100%, respectively after 40 days of injecting Sarcoma-180. The increment of their body weights of the extract feeding groups was suppressed down to 10~15%, compared to the negative control. The nano-particles also reduced the hypertrophy of the internal organs such as spleen and liver down to 15~20%, compared to those as the other groups. Among them, LE effectively reduced the size of tumor form to 20%. From these results, in vitro and in vivo antitumor activities of E. sinica could be enhanced by using nano-encapsulation process with lecithin because of better permeation into the cancer cells by confocal observations.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.4
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• pp.222-229
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• 2005

A simplified one-dimensional model STELLA was used to predict soil water movement in lllinois corn fields using soil water balance sheets. It offered the potential to increase understanding of soil nitrate and agrochemical leaching process. The model accounted for aU possible annual inputs and outputs of water from a closed ecosystem as represented by corn fields. Water inputs included precipitation, while outputs included runoff, transpiration, evaporation and drainage. To run the model required daily inputs of two climatic data measurements such as daily precipitation and pan evaporation. Vertical water flow through the soil profile was calculated with first order equation including the difference in hydraulic conductivity and matric potential at the various soil types. The output results included daily changes of water content in the soil layers and daily amount of water losses including run-off, percolation, transpiration. This model was verified using Illinois corn field data for the soil water content measured by neutron scattering methods through 1992 to 1994 growing seasons. Approximately 22 to 78% of simulated water contents agreed with the measured values and their standard deviation, depending on soil types, whereas 30 to 70% of simulated water values agreed with the measured values and their standard deviations depending on soil layers.

• Animal Systematics, Evolution and Diversity
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• v.14 no.4
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• pp.327-334
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• 1998

Four external and 27 cranial characters of three subspecies of striped field mice (Apodemus agrarius) from 15 regions in far eastern Asia(South Korea, North Korea, northeastern China, and far eastern Russia) were analyzed by multivariate methods in order to determine taxonomic status of striped field mice from North Korea. Three subgroups were recognized: a large-size form from two localities in South Korea(Cheju Island, Wando Island): a medium-size form from eight localities in South Korea and North Korea(Jindo Island, Kunsan, Mt. Chirisan, Cheongju, Mt. Weoleksan, Mt. taebaksan; Haeju, Sineuiju); a small-size form from five localities in North Korea, northeastern China, and far eastern Russia (Mt. Kumkangsan, Mt. Myohyangsan; Jirin, Yichun; Vladivostok). It is confirmed that the large-size form, the medium-size form, and the small-size form are subspecies chejuensis, coreae, and manchuricus, respectively, as noted by Koh et al.(1997). It is revealed that striped field mice(the medium-size form) from western region of North Korea (Haeju, Sineuiju) is classified into A. agrarius coreae, as noted by Thomas(1908), but striped field mice(the small-size form) from eastern region of North Korea(Mt. Kumkangsan, Mt. Myohyangsan) is A. agrarius manchuricus.

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.24-34
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• 1985

To estimate the fundamental situation of weed emergence and it's competition to paddy rice (critical competition period and threshold level) in the southern paddy area in Korea, two rice cultivars (Pungsanbyo and Tongjinbyo) under two cropping periods (Mono- and After-barley-cropping) were experimented. As a result, the dominant weed species (Monochoria vaginalis and Scirpus juncoides Roxb.) and the better emerging conditions (rather at the monocropping and cv. Punsanbyo) were identified. Also, the main components of yield decrease (25-55% of the weed-free check) of both cultivars as affected by weed competition were the number of spikelets, panicle and ripening rate in monocropping, and the ripening rate and grain weight in after-barley-cropping, respectively. Under the situation of combinated dominance with Monochoria v. and Scirpus j. among others, the duration of critical competition periods was enlarged.

• Weed & Turfgrass Science
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• v.1 no.4
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• pp.6-12
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• 2012

A fact-finding survey was conducted to obtain the basic information on nation-wide weed distribution and farmer's opinions to weed control strategy in paddy field. Most respondents considered Echinochloa spp., Monochloa vaginalis, Sagittaria trifolia, and Scirpus juncoides etc. as dominate weeds in paddy fields. And herbicide resistant weed species were M. vaginalis, S. juncoides, E. oryzicola and S. pygmaea etc. Purchase guidances for herbicide selection, experience of farmers was 34.0% and dealers recommendation was 33.9% at farmer's level. And paddy field herbicides application frequence were one time 15.6% but twice 75.6%. Preferred herbicides were ranked in the following order: oxadizone 12% EC, butachlor 5% GR, butachlor 33% CS, mefenacet pyrazodulfuron-ethyl 21.42% SC, and oxadiargyl 1.7% EC etc. in paddy fields. And in most preferred foliar herbicide were bentazone MCPA 38.6% SL and bentazone cyhalop-butyl 18.5% ME, 48.5% and 22.3%, respectively.

• Korean Journal of Medicinal Crop Science
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• v.21 no.5
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• pp.361-371
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• 2013

This study was performed to determine the antimelanogenic effect and tyrosinase inhibitory activities of anthocyanin rich fraction (AN-SLP) from Liriope platyphylla Wang et Tang seeds. Anthocyanins isolated from L. platyphylla seeds revealed the presence of four major anthocyanin components, which were tentatively identified as delphinidin-3-Oglucoside, delphinidin-3-O-rutinoside, petunidin-3-O-rutinoside, and malvidin-3-O-rutinoside using semipreparative HPLC, $^1H$-NMR, $^{13}C$ NMR, FAB-MS and LC/ES-MS. The inhibitory effect of AN-SLP on tyrosinase activity was studied using in vitro (against mushroom tyrosinase) and ex vivo (against B16 melanoma cell tyrosinase) models. Cellular tyrosinase activity was decreased by AN-SLP treatment in B 16 melanoma cells through dose dependent manner, but AN-SLP did not inhibit mushroom tyrosinase and L-DOPA oxidation directly. AN-SLP showed melanin inhibition by 53.2% at 50 ${\mu}g/m{\ell}$ which was 0.7 times more efficient than the antimelanogenic effect of commercial arbutin and kojic acid (36.5%) also did not show cell toxicity. Additionally, AN-SLP inhibited the activity of ${\alpha}$-glucosidase and the glycosylation of tyrosinase in melanoma cell. The resulting unsaturated glycosylation of tyrosinase makes it unstable and disturb correct transportation. From theses results, we conclude that AN-SLP could be used as anti-melanogenic agent for skin whitening.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.95-102
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• 2006

To evaluate bacteriological water quality of groundwater in Yeungnam Province, samples were taken from 123 locations during summer and 117 locations during winter. The medians of heterotrophic plate counts.(HPCs) were 30 CFU/mL for the summer samples and 40 CFU/ml for the winter, and more than 25% showed HPCs higher than 100 CFU/ml. Coliform bacteria were detected from 46% of the summer samples and 30% of the winter. In these coliform-positive samples, the medians of coliform counts were 20 CFU/ml for the summer samples and 4 CFU/ml for the winter. Genera such as Citrobacter, Enterobacter, Escherichia, Klebsielia, Pantoea, Rahnellia, and Serratia were identified from the coliform isolates; among them, 48% were members of the genus Enterobacter. While E. cloacae, E. amnigenus, and K. pneumoniae were the most frequently isolated species, E. coli was isolated only from 1 location. The coliform counts were positively correlated with the HPCs, which also positively correlated with water temperature. The results of present study provide further insight on the extent of groundwater contamination with coliform bacteria.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.354-360
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• 1998

We isolated three amplified DNA fragments from P. sajor-caju by Polmerase chain reaction (PCR) using the chithin synthase specific primers. Since the sequence analysis of the these fragments showed significant homology to the other known chitin synthase gene, we regarded these cloned fragments as PsCHS1, PsCHS2, and PsCHS3 according to their size. The PsCHS3, which showed the highest sequence homology (83% identity in amino acid level with ChsI of Rhizopus oligosporus in conserved region), was selected to see expression pattern of the corresponding gene. The result of RT-PCR using internal primer of the PsCHS3 fragment revealed that PsCHS3 gene was only expressed in cap and mycelium but not in stipe. In order to see whether the PsCHS3 gene was to be induced by wounding, the comparison of the mRNA level of this gene between wounded and unwounded mature cap showed at least two times induction of this gene by wounding treatment.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.19-23
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• 2002

This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid 'Hae Hwa'. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.6
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• pp.1530-1537
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• 2006

Platycodi Radix, the root of Platycodon grandiflorum A. DC (Campanulaceae), commonly known as Doraji in Korea (Chinese name, 'Jiegeng', and Japanese name, 'Kikyo') has been used as an expectorant in traditional Oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health benefits. In Korea, Platycodi Radix is also used as a food and employed as a folk remedy for adult diseases, such as bronchitis, asthma and pulmonary tuberculosis, hyperlipidemia, diabetes, and inflammatory diseases, and as a sedative. Several studies on its chemical and immunopharmacological effects including immunostimulation and antitumor activity have been performed. However, the relevant molecular mechanisms are poorly understood. Platycodi Radix, the root of Platycodon grandiflorum A. DC (Campanulaceae), commonly known as Doraji in Korea (Chinese name, 'Jiegeng', and Japanese name, 'Kikyo') has been used as an expectorant in traditional Oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health bensfits. In Korea, Platycodi Radix is also used as a food and employed as a folk remedy for adult diseases, such as bronchitis, asthma and pulmonary tuberculosis, hyperlipidemia, diabetes, and inflammatory diseases, and as a sedative. Several studies on its chemical and immunopharmacological effects including immunostimulation and antitumor activity have been performed. However, the relevant molecular mechanisms are poorly understood. In the present study, we investigated the effects of an aqueous extract from the roots of P. grandiflorum AEPG) on the cell growth of human lung adenocarcinoma NCI-H460 cells in order to understand its anti-proliferative mechanism. AEPG treatment down-regulated the cyclin D1 expression in both transcriptional and translational levels without alteration of cyclin E. In AEPG-treated cells, the levels of cyclin-dependent kinase (C아) 6 mRNA and protein were significantly inhibited, but the levels of Cdk2 and Cdk4 were slightly inhibited by treatment of AEPG. AEPG treatment induced a marked accumulation of Cdk inhibitors, p16 and p27. However, AEPG treatment did not affect not only retinoblastoma protein (pRB) but also tumor suppressor p53 protein expression. The present results indicated that AEPG-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1 phase progression through induction of Cdk inhibitors such as p16 and p27, and inhibition of cyclin D1 and Cdk6. AEPG exposure, as offered by this study, provides cluse for the mechanism of AEPG action. Taken together, these findings suggest that P. grandiflorum has strong potential for development as an agent for prevention and treatiment against human lung cancer.