• KSBB Journal
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• v.17 no.4
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• pp.370-375
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• 2002

Isolation of a gene and determination of its expression pattern are essential in understanding its function. Among the genes localized in 12ql3, stSG3435 EST was chosen to study its expression pattern. The full-length CDNA was cloned by screening of human brain CDNA library and its sequence was determined by serial deletion followed by automated sequencing of the clones with overlapping fragments. The sequence analysis revealed that stSG 3435 CDNA displayed 100% identity to human MYGI and 86% identity to mouse melanocyte proliferation gene-1 (Gamm 1) originally identified from melanocyte, suggesting that MYGI determined by Northern blot analysis revealed the strongest expression in testes with ubiquitous expression in all the tissues tested. In order to investigate the cellular localization of its protein product, the green fluorescence protein gene was fused into the full-length coding sequence of MYGI, Transfection of the fusion construct followed by confocal microscopy resulted in the green fluorescence signal as a punctate state in cytoplasm indication that MYGI was localized in one of the cellular organelles.

• Computers and Concrete
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• v.13 no.1
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• pp.1-16
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• 2014

To evaluate the behavior of the advanced unbonded pre-stressed concrete containment vessel (UPCCV) for one typical China nuclear power plant under Japan's March 11 earthquake, five nonlinear time history analysis and a nonlinear static analysis of a 1:10 scale UPCCV structure have been carried out with MSC.MARC finite element program. Comparisons between the analytical and experimental results demonstrated that the developed finite element model can predict the earthquake behavior of the UPCCV with fair accuracy. The responses of the 1:10 scale UPCCV subjected to the 11 March 2011 Japan earthquakes recorded at the MYG003 station with the peak ground acceleration (PGA) of 781 gal and at the MYG013 station with the PGA of 982 gal were predicted by the dynamic analysis. Finally, a static analysis was performed to seek the ultimate load carrying capacity for the 1:10 scale UPCCV.