• Title/Summary/Keyword: MT1

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Hyperproduction of L-Threonine by Adding Sodium Citrate as Carbon Source in Transformed Escherichia coli Mutant. (형질전환된 Escherichia coli변이주에서 Sodium citrate를 이용한 고농도 L-Threonine 생산)

  • 이만효;김병진;정월규;최선욱;박해룡;황용일
    • Journal of Life Science
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    • v.14 no.5
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    • pp.868-873
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    • 2004
  • The efficient fermentative production of L-threonine fermentation was achieved by using Escherichia coli MT201, transformed a plasmid carrying pyruvate carboxylase gene. It is an attempt to supply oxaloacetate to the L-threonine biosynthetic pathway. In order to improve the L-threonine productivity of E. coli MT201, a plasmid pPYC which is an expression vector of the pyruvate carboxylase gene of Coryne-bacterium glutamicum, was introduced. When E. coli MT/pPYC was incubated with medium containing only glucose as a carbon source, both the cell growth and L-threonine production were reduced, compared to the results from fermentation of E. coli MT201. In order to circumvent this effect, we attempted the addition of a mixed carbon source, composed of glucose and sodium citrate at a ratio of 1.5:3.5. It was shown that L-threonine production and cell growth (OD660) with E. coli MT/pPYC reached up to 75.7 g/l and 48, respectively, at incubation for 75 hr under fed-batch fermentation conditions. It is assumed that overproduction of L-threonine by anaplerotic pathway leads unbalance of TCA cycle and sodium citrate might playa role to recover normal TCA cycle.

The Basic Study for Developing Plant Ecological Indices of Sacheon-si, Gyeongsangnam-do (경상남도 사천시의 식물생태지수 개발을 위한 기초연구)

  • Oh, Hyun-Kyung;You, Ju-Han
    • Journal of Environmental Impact Assessment
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    • v.21 no.4
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    • pp.509-523
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    • 2012
  • This basic study is to present the methods and raw data for carrying out to objectively assess an ecosystem by developing the plant ecological indices. The results are as follows. The plant ecological indices were 7 units including RI (Rare Index), EI (Endemic Index), SI (Specific Index), NI (Naturalized Index), UI (Urbanized Index), Pte-Q (Pteridophyta Index) and HI (Halophytes Index). The vascular plants in Mt. Waryong, Mt. Bongmyeong and Bito island were summarized as 471 taxa including 104 families, 294 genera, 419 species, 3 subspecies, 41 varieties and 8 forms. We surveyed 5 taxa of rare plants, 7 taxa of endemic plants, 38 taxa of specific plants by floristic region, 40 taxa of naturalized plants, 1 taxa of invasive alien plant, 19 taxa of pteridophyta and 14 taxa of halophytes. Bito island had the highest RI was 1.2%. Mt. Bongmyeong was 1.4% higher than other sites. If the SI of 2.9% was the highest in Bito island. Bito island was the highest NI, and the UI was the hightest in Mt. Waryong. Mt. Bongmyeong was the lowest NI and UI. In Mt. Waryong and Bito island, the highest DI was 9.1%. The Pte-Q of Mt. Waryong was 1.3 higher than the other sites. Bito island, the highest HI was 8.1%. Overall, 1.5% of RI, 1.9% of EI, 2.3% of SI, 8.5% of NI, 14.3% of UI, 9.1% of DI, 1.0 of Pte-Q and 3.0% were analyzed with HI.

Characteristics of Rice Mutants Resistant to 5- Methyltryptophan (벼 5-methyltryptophan 저항성 돌연변이체의 특성)

  • 이효연;강권규;노일섭;이춘환;권혜경;박현숙
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.5
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    • pp.637-643
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    • 1995
  • TR75, a rice (Oryza sativa L. var. Sasanishikj) mutant resistant to 5-methyltryptophan (5MT) was segregated from the progenies of its initial mutant line, TR1. The 5MT resistance of TR75 was inherited in the M$_{8}$ generations as a single dominant nuclear gene, and was also expressed in callus derived from seeds, roots, and anthers as well as in the seedlings. The callus induced from these organs could grow at 50 mg/1 of 5MT, whereas the growth of wild-type callus was completely inhibited even at 25 mg/1. The seedlings of TR75 did not show resistance to L-azetidine-2-carboxylic acid, S-2-aminoethyl-L-cysteine, p-fluoro-DL-phenylalanine. The content of free amino acids in the TR75 homozygous seeds increased approximately 1.5 to 2.0 fold compared to wild-type seeds. Especially, the contents of tryptophan, phenylalanine and aspartic acid were 5.0, 5.3 and 2.7 times higher than those of wild-type seeds, respectively.y.

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Influences of the devastated forest lands on flood damages (Observed at Chonbo and the neighbouring Mt. Jook-yop area) (황폐임야(荒廢林野)가 수해참상(水害慘狀)에 미치는 영향(影響) (천보산(天寶山)과 인접(隣接) 죽엽산(竹葉山)을 중심(中心)으로))

  • Chung, In Koo
    • Journal of Korean Society of Forest Science
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    • v.5 no.1
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    • pp.4-9
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    • 1966
  • 1. On 13 September 1964 a storm raged for 3 hours and 20 minutes with pounding heavy rainfalls, and precipitation of 287.5 mm was recorded on that day. The numerous landslides were occured in the eroded forest land neighbouring Mt. Chunbo, while no landslides recorde at all on Mt. Jookyup within the premise of Kwangnung Experiment Station, the Forest Experiment Station. 2. Small-scalled Landslides were occured in 43 different places of watershed area (21.97 ha.) in which the survey had already been done, in and around Mt. Chunbo (378 m a.s.l.). The accumulated soil amount totaled $2,146,56m^3$ due to the above mentioned landslides, while soil accumulated from riverside erosion has reached to $24,168.79m^3$, consisting of soils, stones, and pebbles. However, no landslides were reported in the Mt. Jook yup area because of dense forest covers. The ratio of the eroded soil amount accumulated from the riversides to that of watershed area was 1 to 25. On the other hand, the loss and damage in the research area of Mt. Chonbo are as follows: 28 houses completly destroyed or missing 7 houses partially destroyed 51 men were dead 5 missing, and 57 wounded. It was a terrible human disaster However, no human casualties were recorded at all, 1 house-completly destroyed and missing, 2 houses-partially destroyed. Total:3 houses were destroyed or damaged, in The area of Mt. Jookyup 3. In the calculation of the quanty of accumulated soil, the or mula of "V=1/3h ($a+{\sqrt{ab}}+b$)" was used and it showed that 24, 168.79m of soil, sands, stones and pebbles carried away. 4. Average slope of the stream stood 15 at the time of accident and well found that there was a correlation between the 87% of cross-area sufferd valley erosion and the length of eroded valley, after a study on regression and correlation of the length and cross-area. In other works, the soil erosion was and severe as we approached to the down-stream, counting at a place of average ($15^{\circ}1^{\prime}$) and below. We might draw a correlation such as "Y=ax-b" in terms of the length and cross-area of the eroded valley. 5. Sites of char-coal pits were found in the upper part of the desert-like Mt. Chunbo and a professional opinion shows that the mountain was once covered by the oak three species. Furthermore, we found that the soil of both mountains have been kept the same soil system according to a research of the soil cross-area. In other words, we can draw out the fact that, originally, the forest type and soil type of both Mt. Chunbo (378m) and Mt. Jookyup (610m) have been and are the same. However, Mt. Chunbo has been much more devastated than Mt. Jookyup, and carried away its soil nutrition to the extent that the ratios of N. $P_2O_5K_2O$ and Humus C.E.C between these two mountains are 1:10;1:5 respectively. 6. Mt. Chunbo has been mostly eroded for the past 30 years, and it consists of gravels of 2mm or larger size in the upper part of the mountain, while in the lower foot part, the sandy loam was formulated due to the fact that the gluey soil has been carried and accumulated. On the hand, Mt. Jookyup has consitantly kept the all the same forest type and sandy loam of brown colour both in the upper and lower parts. 7. As for the capability of absorbing and saturating maximum humidity by the surface soil, the ratios of wet soil to dry soil are 42.8% in the hill side and lower part of the eroded Mt. Chunbo and 28.5% in the upper part. On the contrary, Mt. Jookyup on which the forest type has not been changed, shows that the ratio in 77.4% in the hill-side and 68.2% in the upper part, approximately twice as much humidity as Mt. Chunbo. This proves the fact that the forest lands with dense forest covers are much more capable of maintaining water by wood, vegitation, and an organic material. The strength of dreventing from carring away surface soil is great due to the vigorous network of the root systems. 8. As mentioned above, the devastated forest land cause not only much greater devastation, but also human loss and property damage. We must bear in mind that the eroded forest land has taken the valuable soil, which is the very existance of origin of both human being and all creatures. As for the prescription for preventing erosion of forest land, the trees for furtilization has to be planted in the hill,side with at least reasonable amount of aertilizer, in order to restore the strength of earth soil, while in the lower part, thorough erosion control and reforestation, and establishments along the riversides have to be made, so as to restore the forest type.

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Study on the Genetic Variation of the Mitochondrial DNA in the Beet Armyworm, Spodoptera exigua (H bner), Using PCR-RFLP (PCR-RFLP를 이용한 파방나방 (Spodoptera exigua(H bner)) 미토콘트리아 DNA의 유전변이 연구)

  • 김용균;이명렬;정충렬
    • Korean journal of applied entomology
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    • v.37 no.1
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    • pp.23-30
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    • 1998
  • Restriction fragment length polymorphism (RFLP) of a DNA has been a useful tool for analyzing genetic variation. This research was performed to establish an RFLP analytic method on the mitochondrial DNA (mtDNA) of the beet armyworm, Spodoptera exigua (Hiibner). To do this, total size of the mtDNA was measured and polymerase chain reaction (PCR) primers were selected. Its mitochondrial genome size was ca. 16kb. From a serial PCR test of 29 primers refered to the compilation of Simon et al. (1994), 22 primers were selected to amplify its mtDNA fragments. These primers resulted in short (300-700 bp) or long (1000-2000 bp) DNA products which represented a total or partial sequence of each of CO-I, CO-11, Cyt-B, ND-1, 12s rRNA, 16s rRNA, and some tRNAs. PCR-RFLP was performed in some variable mtDNA regions with 8 kinds of 4bp recognizing restriction enzymes. Different populations from Andong, Kyungsan, and Sunchun did not show any restriction site polymorphisms but had some length variation in certain regions of mtDNA.

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Enhancement of cadmium resistance by overexpression of BrMT3 in Arabidopsis (BrMT3 고발현에 의한 애기장대의 카드뮴 저항성 증진)

  • Kim, Sun-Ha;Song, Won-Yong;Ahn, Young-Ock;Lee, Haeng-Soon;Kwak, Sang-Soo;Choi, Kwan-Sam
    • Journal of Plant Biotechnology
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    • v.36 no.1
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    • pp.68-74
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    • 2009
  • We have previously demonstrated that overexpression and characterization of Brassica rapa type-l metallothionein gene (BrMT1) in Arabidopsis which showed enhanced resistance to cadmium and ROS. Here, we present the consistent study of our previous report about BrMTs. BrMT3 expressing DTY167 cells showed resistance to Zn and Pb as well as Cd. Thus, we have developed the BrMT3 overexpression Arabidopsis to enhance capacity for metal stresses. Successful expression and localization were achieved using the rubisco transit peptides of RbcS-BrMT3-GFP protein, which was confirmed by western blot analysis with the GFP antibody and green fluorescence signal from the chloroplast. BrMT3 overexpression Arabidopsis plants exhibited a higher resistance to cadmium compared to control plants. This result indicates that BrMT3 would be applicable to the development of plants with enhanced resistance against heavy metal stresses.

Vegetation Structure of Picea jezoensis Communities in Mt. Deogyu and Mt. Gyebang (덕유산과 계방산 가문비나무 군락의 식생구조에 관한 연구)

  • Cho, Min-Gi;Chung, Jae-Min;Jung, Hye-Ran;Kang, Mee-Young;Moon, Hyun-Shik
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.33-41
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    • 2012
  • This study was conducted to provide the informations for conservation and effective management of Picea yezoensis community in Mt. Deogyu and Mt. Gyebang. The vegetation of tree, subtree and shrub layer was consist of 8, 20, 26 species in Mt. Deogyu, and 12, 23, 33 species in Mt. Gyebang. Importance value by layer P. yezoensis, Betula ermanii, Abies koreana at tree layer, B. ermanii, Quercus mongolica at subtree layer, and Sasa borealis at shrub layer in Mt. Deogyu, and P. yezoensis, B. ermanii, Abies nephrolepis at tree layer, Acer komarovii and A. ukurunduense at subtree layer, and Tripterygium regelii at shrub layer in Mt. Gyebang were high, respectively. Species diversity in Mt. Deogyu and Mt. Gyebang were 0.779 and 0.984 at tree layer, 1.052 and 1.161 at subtree layer, and 0.823 and 1.304 at shrub layer, respectively. According to the DBH class of major species, P. yezoensis in Mt. Deogyu showed a reverse J-shaped curve, which was estimated that P. yezoensis community of this site might be maintained continuously as a stable state.

Infection with Citrobacter rodentium in μMT Knockout Mice

  • Jo, Minjeong;Hwang, Soonjae;Rhee, Ki-Jong
    • Biomedical Science Letters
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    • v.24 no.1
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    • pp.1-8
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    • 2018
  • ${\mu}MT$ knockout mice are genetically deficient in the transmembrane domain of mu chain of the immunoglobulin M (IgM) heavy chain, resulting in the absence of mature B cells. ${\mu}MT$ knockout mice is an in vivo model system used to clarify the role of B cells in various diseases. Enteropathogenic Escherichia coli (EPEC) induces acute and chronic diarrheal disease, especially in children of developing countries. The formation of attaching and effacing (A/E) lesion is a prominent pathogenic factor in the intestinal epithelium of EPEC infection. The A/E lesion is modulated by genes located on the pathogenic island locus of enterocyte effacement (LEE) which encode a type III secretion system (T3SS) and A/E lesion-related effector proteins. Citrobacter rodentium is a murine pathogen utilized in studying the pathogenic mechanisms of EPEC in human infections. Citrobacter rodentium produce A/E lesion to attach to intestinal epithelium, thus providing a murine model pathogen to study EPEC. Several studies have investigated the pathogenesis of Citrobacter rodentium in the ${\mu}MT$ knockout mice. In this review, we introduce the ${\mu}MT$ murine model in the context of C. rodentium pathogenesis and describe in detail the role of B cells and antibodies in this disease.

Gene Therapy for Mice Sarcoma with Oncolytic Herpes Simplex Virus-1 Lacking the Apoptosis-inhibiting Gene, icp34.5

  • Lan, Ping;Dong, Changyuan;Qi, Yipeng;Xiao, Gengfu;Xue, Feng
    • BMB Reports
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    • v.36 no.4
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    • pp.379-386
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    • 2003
  • A mutant herpes simplex virus 1, mtHSV, was constructed by inserting the E. coli beta-galactosidase gene into the loci of icp34.5, the apoptosis-inhibiting gene of HSV. The mtHSV replicated in and lysed U251 (human glioma cells), EJ (human bladder cells), and S-180 (mice sarcoma cells), but not Wish (human amnion cells) cells. With its intact tk (thymidine kinase) gene, mtHSV exhibited susceptibility to acyclovir (ACV), which provided an approach to control viral replication. An in vivo test with mtHSV was conducted in immune-competent mice bearing sarcoma S-180 tumors, which were treated with a single intratumoral injection of mtHSV or PBS. Tumor dimensions then were measured at serial time points, and the tumor volumes were calculated. Sarcoma growth was significantly inhibited with prolonged time and reduced tumor volume. There was microscopic evidence of necrosis of tumors in treated mice, whereas no damage was found in other organs. Immunohistochemical staining revealed that virus replication was exclusively confined to the treated tumor cells. HSV-1 DNA was detected in tumors, but not in the other organs by a polymerase chain reaction analysis. From these experiments, we concluded that mtHSV should be a safe and promising oncolytic agent for cancer treatment.

Carbon Storage of Quercus mongolica Stands by Latitude and Altitude in Korea

  • Kwon, Ki-Cheol;Lee, Don Koo
    • Journal of Korean Society of Forest Science
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    • v.95 no.2
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    • pp.220-231
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    • 2006
  • The study was conducted to investigate the differences in carbon storage of Quercus mongolica stands with respect to latitude and altitude in Korea. Study sites were located in Mt. Joongwang, Pyeongchang-gun, Gangwon-do (altitudes 1,300 m, 1,000 m, and 800 m), Mt. Taehwa, Gwangju-si, Gyeonggi-do (altitude 350 m), Mt. Wolak, lecheon-si, Chungcheongbuk-do (altitude 300 m), Mt. Baekwoon, Gwangyang-si, Jeollanam-do (altitude 800 m), and Mt. Halla, Jeju-do (altitude 1,000 m). Total carbon storage and annual carbon storage of Q. mongolica stands were 85-210 tonC/ha and 7.2-10.6 tonC/ha, respectively. Lower latitude (NE) stands of Q. mongolica showed more carbon storage and annual carbon storage than higher latitude stands. Carbon storage and annual carbon storage of Q. mongolica stands were increased in low altitude. Carbon storage of Q. mongolica stands was higher in the northern aspect than in the southern aspect. However, there were no significant differences in annual carbon storage between the aspects.