• Food Science and Preservation
• /
• v.24 no.2
• /
• pp.274-281
• /
• 2017

The optimization of lactic acid fermentation was conducted to produce an old antler fortified with functional ingredients. For the over-production of gamma aminobutyric acid (GABA), the extract of old antlers (OA) was fermented by Lactobacillus plantarum EJ2015 with 0.5% YE, 1.5% glucose, and 3.5% MSG at $30^{\circ}C$ for 7 days. The lactic acid fermented OA showed high viable cell counts of $2.0{\times}10^8CFU/mL$, pH 6.56 and 0.77% acidity after 7 days. Addition of Auricularia auricula-judae (AAJ) enhanced the cell growth of L. plantarum EJ2014, resulting in higher viable cell counts of $2.0{\times}10^9CFU/mL$ and acid production after fermentation for 1 day. In particular, acidity was greatly decreased after fermentation for 3 days and 1.4% GABA was produced by converting efficiently mono sodium glutamate as a substrate. Fermented OA/AAJ mixture indicated the reduced cytotoxicity compared with that of unfermented OA. The fermented OA/AAJ mixture indicated anti-inflammatory effect with less production of NO in microphage cells. The production of NO dropped to $17.75{\mu}M$ at 4 mg/mL, and to $5.58{\mu}M$ at 6 mg/mL old antler after fermentation. Thus, lactic acid fermented OA with AAJ could fortify GABA, probiotics and dietary fiber.

• Food Industry
• /
• s.181
• /
• pp.68-91
• /
• 2004

조미료란 식품을 조리, 가공, 섭취할 때 맛을 증진시키거나 강화시킬 목적으로 사용되는 물질을 일컫는다. 발효, 종합 및 천연계 조미료의 국내외 시장동향에 대해 살펴보고, 조미료의 향후 발전방향과 중점 연구 분야, 관련업계의 연구개발 동향에 대한 고찰을 토대로 조미료 분야의 고부가가치 산업으로의 육성방안에 대해 알아보았다. 미원, 아이미 등으로 대표되는 발효조미료의 내수시장은 성숙기를 거쳐 소폭 감소세를 보이는 쇠퇴기에 접어들고 있다. 이는 소비자의 MSG에 대한 기피현상이 주 원인으로 판단된다. 전반적으로 품질 차별화 없이 가격 경쟁이 심화되고 있는 실정이다. 국내 종합조미료 시장은 1975년 국내에 처음으로 CJ에서 다시다 제품을 출시한 후 대상에서 1982년 맛나, 1988년 감치미를 출시하면서 30여 년간 지속적으로 성장해 왔다. 현재 시장규모는 2천억원에 이르고 있으며 최근 들어서는 성장세가 완만해지고 있고 가정용은 거의 정체되어 있다. 일본 조미료 시장은 발효조미료, 종합조미료 그리고 다양한 조미식품의 소재로 활용되는 천연조미료로 구분할 수 있다. 발효조미료 시장은 국내와 비슷한 상황으로 전체적인 물량변화는 약간 감소하는 경향이며 생산기지의 해외이전 현상이 뚜렷하다. 종합조미료 시장은 한국과 유사한 풍미조미료 외에도 보다 전문화되고 다양한 형태의 조미료가 시판되고 있으며, 전통적인 풍미조미료는 정체현상을 보이고 건강지향, 전용화 조미료가 호조를 보이고 있다. 일본과 한국의 조미료 발전 단계는 조금 차이를 보이고 있다. 현재 한국은 종합조미료 시대에서 용도별 조미료 시대로 넘어가는 단계에 있는 반면, 일본은 이미 메뉴 전용 조미료시대에 접어들었고, 건강, 기능성을 강조하는 시대로 나아가고 있다. 이들 변화는 건강에 대한 소비자의 관심과 그로 인한 보다 천연에 가까운 조미소재에 대한 수요, 동시에 맛에 대한 충족까지 요구하는 시대적 변화에 따른 필연적 결과로 보인다. 미래의 조미료는 천연계 소재의 사용량이 증가할 것이고, 소비자의 건강, 안전 추구심리에 부흥하여 안전성과 기능성을 부여하는 소재에 관심이 집중될 것으로 보인다. 더불어 전반적인 생활수준 향상에 따라 제품의 고급화와 전용화도 진행될 것으로 보인다. 향후 조미료시장을 주도할 각 현상에 대해 좀더 자세히 알아보고 학계 및 업체의 최근 연구동향과 제품개발 현황에 대해 고찰해 보았다. 기술연구 동향을 보면, 나노 기술에 의한 조미신소재연구, 초미분쇄기술, 미세 캡슐화 기술, 생물공학 기술이 활용된 발효 및 효소분해에 의한 펩타이드계 조미소재 개발 등이 활발하게 진행되고 있다. 선진업체 연구 및 제품개발 동향은 아지노모트/교와의 ''코쿠미''계 조미소재 연구개발, 일연푸드/동해물산의 천연 엑기스계 조미소재 연구개발, 보조주(주)의 주류 조미료 개발 위주로 살펴보았다. 조미료 시장의 발전 과정과 선진 업체들의 연구, 개발동향을 종합해 보면 첫째는 천연 건강지향에 부응하는 차별화된 소재 및 기능성 소재 개발이고 둘째는 맛과 편리함의 추구에 대응하는 메뉴 전용화 추세이다. 이를 위한 기술적 연구도 기능성 소재(히스티딘 함유 디펩타이드)개발, 나노기술, 미세캡슐화 기술 등을 통하여 다양하게 진행되고 있다. 조미료 산업을 첨단 고부가가치 산업으로 육성하기 위해서는 R&D 투자확대와 효율성을 제고하는 것이 필수적이다. 각 기업단위에서는 장기적 차원에서 첨단식품 기반기술에 대한 연구를 수행하거나, 전문 중소기업, 벤처를 육성하는데 지원해야 한다. 그러나 현실은 당장의 수익과 회사간 경쟁으로 인해 장기적 투자, 사회적 인프라 육성 등에는 관심을 쏟지 않고 있다. 조미료를 비롯한 식품에 관련된 기술들이 다양한 기초연구와 응용기술, 개발연구 등의 총합인 경우가 많기 때문에 이들 상이한 연구 분야간의 연계를 강화하고 개발된 기술의 이전, 확산체계를 구축할 필요가 있다. 이를 위해 기업간 수평적 네트워크 외에도 국가기관이 참여하는 연구 자원의 효율적 집행 기관이 필요하리라 본다.

• Korean Journal of Food Science and Technology
• /
• v.26 no.4
• /
• pp.393-397
• /
• 1994

The study was carried out to develop the powdered smoked-dried anchovy products as a natural flavoring substance. The processing conditions, chemical and taste compounds of products were as follows: The raw anchovy were washed, and then boiled 5 minutes in $5{\sim}6%$ NaCl and 1.0% sodium erythorbate solution. Boiled anchovy were smoked in smoking house at $40^{\circ}C$ for 4 hours as the first stage, and then increased temperature up to $80^{\circ}C$ as the second stage, and finally smoked 8 hrs at $80^{\circ}C$ to maintain the moisture content between 9 and 10 percent. The smoked-dried anchovy were pulverized and screened to be 50 mesh of particle size, and finally packed in PET/Al/CPP film bag. The moisture, crude lipid content and salinity of powdered smoke-dried anchovy were 9.4%, 9.6% and 6.9%, respectively. Fatty acid composition of product was mainly consisted of polyenes (43.4%) such as 22 : 6 and 20 : 5, followed by saturates (36.9%), monoenes (19.7%). The principal taste compounds of product were IMP, 466.5 mg/100g; free amino acids such as His, Tau, Pro, Lys, Ala and Glu, 1179.2 mg/100g; non-volatile organic acids such as lactic acid and succinic acid, 617.9 mg/100g; total creatinine, 595.9 mg/100g; small amount of betaine and TMAO. To make a instant soup, it was desirable for taste of products that powdered smoked-dried anchovy were mixed with 20% salt, 4.0% sugar, 3.0% MSG, 1.0% onion powder, 1.0% garlic powder and 1.0% black pepper.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.17 no.5
• /
• pp.368-372
• /
• 1984

This paper deals with a rapid method for determination of ATP and its related compounds in fish and shellfish products using high performance liquid chromatography(HPLC). The HPLC used is a HPLC/ALC-224 equiped with UV-spectrophotometer (254 nm) as detector and integrator (Yanagimoto system-1000). The column used is a stainless steel tubing ($30.0\;cm{\times}3.9\;mm\;i.d.$) packed with ${\mu}-Bon-dapak\;C_{18}$. A mixture of $1\%$ triethylamine-phosphoric acid(pH6.5) was used as an eluent and the flow rate of the eluent was controlled at 2 ml/min. For the separation of ATP and its related compounds, a standard mixture of ATP, ADP, AMP, IMP, inosine and hypoxanthine was subjected to HPLC under the above mentioned conditions. Six peaks were obtained with retention times within 20 min, and elution order were hypoxanthine, IMP, inosine, AMP, ADP and ATP. But 5'-IMP and 5'-GMP fractions were not separated by this method. In generally, IMP content in boiled-dried fish and shellfish products purchased from the market was comparatively higher than that of other nucleotides. Especially, boiled-dried big eye herring marked higher value in IMP content than other boiled-dried ones. Hypoxanthine and inosine were major components of ATP-related compounds in dried products and seasoned-dried ones. And IMP content in seasoned-dried products was higher than that of dried ones. This fact is suggested that a part of IMP in seasoned-dried ones was derived from flavoring matter (MSG, 5'-IMP and 5'-GMP) which is added during the seasoning treatment.

• Journal of electromagnetic engineering and science
• /
• v.1 no.1
• /
• pp.73-77
• /
• 2001

We studied the fabrication of GaAs-based pseudomorphic high electron mobility transistors(PHEMT`s) for the purpose of millimeter- wave applications. To fabricate the high performance GaAs-based PHEMT`s, we performed the simulation to analyze the designed epitaxial-structures. Each unit processes, such as 0.1 m$\mu$$\Gamma$-gate lithography, silicon nitride passivation and air-bridge process were developed to achieve high performance device characteristics. The DC characteristics of the PHEMT`s were measured at a 70 $\mu$m unit gate width of 2 gate fingers, and showed a good pinch-off property ($V_p$= -1.75 V) and a drain-source saturation current density ($I_{dss}$) of 450 mA/mm. Maximum extrinsic transconductance $(g_m)$ was 363.6 mS/mm at $V_{gs}$ = -0.7 V, $V_{ds}$ = 1.5 V, and $I_{ds}$ =0.5 $I_{dss}$. The RF measurements were performed in the frequency range of 1.0~50 GHz. For this measurement, the drain and gate voltage were 1.5 V and -0.7 V, respectively. At 50 GHz, 9.2 dB of maximum stable gain (MSG) and 3.2 dB of $S_{21}$ gain were obtained, respectively. A current gain cut-off frequency $(f_T)$ of 106 GHz and a maximum frequency of oscillation $(f_{max})$ of 160 GHz were achieved from the fabricated PHEMT\\`s of 0.1 m$\mu$ gate length.h.

• Journal of the Korean Society of Food Culture
• /
• v.23 no.1
• /
• pp.1-9
• /
• 2008

This study aimed to offer some basic data for nutrition education to housewives by comparing behaviors of eating out targeted on housewives who live in Daejon surveyed by classification of regions and by family forms. As for their ages, 79 housewives (16.0%) are less than 30, 179 housewives (36.8%) are between 30 and 39, 156 housewives (35.9%) are between 40 and 49 and 74 housewives (15.2%) are more than 50, and as for their final academic careers, 204 housewives (41.9%) graduated from universities, which was the most and as for religions, 172 housewives (35.3%) had no religion, which was the most, and in the case that they had religions, 137 housewives (27.5%) believed in Christianity. And as for occupations, 327 housewives (69.0%) had no job, and in case they had jobs, 123 housewives (25.3%) that occupied the most were professional. As for husbands’ occupations, profession was the most for 143 housewives (29.4%) and as for monthly income, $2\; million\;won{\sim}3\;million$ won was most as 40.0%. As for times of eating out, ‘4 times a year’ was said by 131 housewives (26.9%), which was the most, and as for the times of food home service, ‘seldom’ was said by 221 housewives (45.4%), and as to the expense for eating out a month, ‘less than 100,000 won’ was said by 219 housewives (45.0%), which was the most, and as for the reasons of their eating out, ‘for harmony in a family’ was said by 119 housewives (24.4%). As for times of eating out by general items of housewives, it was appeared that the younger, the higher in education, less in religion, more in occupation, the more professional in their husbands’ jobs, the more nuclear in family forms, fewer in number of family members and the more average family income per a month, they had more times of eating out. Regarding the factors influencing on eating out, ‘high price’, ‘nutritive imbalance’ and ‘use of MSG’ were pointed by 204 housewives (41.9%), 83 housewives (17.0%) and 63 housewives (12.9%) respectively as the problems of food service shops. And as for the question why their went to their regular restaurants, 185 housewives (38.0%) said ‘taste of food’, and the most considerable thing when they selected menu at a restaurant was ‘taste of food’ for 167 housewives (34.3%). The survey showed that a lot of housewives thought the prices at restaurants were high, and they valued taste of food most, therefore food service shops should improve the taste and nutrition of food.

• Journal of Life Science
• /
• v.28 no.2
• /
• pp.223-228
• /
• 2018

Lactic acid bacteria were isolated from a variety of fermented seafoods and sea creatures from the East Sea Rim, Korea and were screened for ${\gamma}-amino$ butyric acid-producing (GABA) activity. Through a 16S rRNA sequence analysis, the bacteria of interest, which were GABA-positive on the thin-layer chromatography analysis, were recognized as three isolates of Lactobacillus (Lb.) brevis and one isolate of Lactococcus (Lc.) lactis. Lb. brevis FSFL0004 and FSFL0005 were isolated from fermented anglerfish and Lb. brevis FSFL0036 was derived from salted cutlass fish. The Lc. lactis strain FGL0007 was isolated from the gut of a brown sole flounder. According to HPLC analysis, the GABA contents produced by FSFL0004, FSFL0005, FSFL0036 and FGL0007 were equivalent to $10,754.37{\mu}g/ml$, $13,082.79{\mu}g/ml$, $12,290.19{\mu}g/ml$, and $45.07{\mu}g/ml$ respectively in 1% monosodium glutamate-supplemented methionyl-tRNA synthetase (MRS) broth. The four strains were inoculated in skim milk with 1% monosodium glutamate to commercialize the strains as starter cultures for GABA-enriched dairy products, and TLC results displayed the production of ${\gamma}-amino$ butyric acid by all four strains in the adaptation media. Lc. lactis FGL0007 demonstrated the greatest GABA production ($431.42{\mu}g/ml$) by HPLC analysis. The GABA production by lactic acid bacteria strains in the skim milk demonstrated in the present study may be helpful for the production of GABA-enriched dairy products.

• Food Science of Animal Resources
• /
• v.29 no.4
• /
• pp.437-444
• /
• 2009

${\gamma}-Aminobutyric$ acid (GABA) producing lactic acid bacteria, Lactobacillus acidophilus RMK567 was cultivated in 50 L of sterilized MRS broth using a fermenter at $40^{\circ}C$ for 24 h. The cell number was increased to $10.04{\pm}0.13$ Log CFU/mL with a growth rate constant (k) of 0.454 generation/h and a generation time (g) of 2.303 h after a lapse of a lag phase (L) of 5.16 h. A total of 487 g of cell paste with 40.5% moisture was harvested with viable cell number of 12.48 Log CFU/g cell paste. The cell pastes after preparation with glycerol, glucose, and polydextrose as cryo-protectants were lyophilized under a vacuum of 84 m torr. A total of 408 g of freeze dried (FD) cell powders were mixed with a commercial strain of Streptococcus thermophilus to prepare of three types FD starter cultures with the viable cell numbers of 12.42 (FDA-GY), 12.60 (FDBGG) and 12.91 (FDC-GP) Log CFU/g. During preservation the FD cultures at -$18^{\circ}C$, the cell viability of the FD starter cultures were rapidly dropped to below 3.24% of the day of storage. No significant difference was found in the cell viabilities among three types of FD starters cultures, but significant difference (p<0.01) was found in storage periods. Yoghurts fermented through FD starter culture of L. acidophilus RMK567 were determined to contain $155.16{\pm}8.53$ ppm, $243.82{\pm}4.27$ ppm, and $198.64{\pm}23.46$ ppm of GABA, respectively. This study shows that GABA production activity of L. acidophilus RMK567 is not affected during the freeze drying process and would be available for commercial production of yoghurt containing high GABA content.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.42 no.2
• /
• pp.203-211
• /
• 2013

The purpose of this study was to investigate the perception of aseptic foods (sterilized foods with radiation technology) in cancer patients in Busan. The subjects were 192 cancer patients at a hospital. This study was performed through interviews using questionnaires. Patients had perception scores on the need to develop aseptic foods (4.30 points out of 5.00), use aspetic foods (4.06/5.00), and intake aseptic foods (4.04/5.00). Patients less than 59 years old had significantly higher perception scores than those over 60 years old (p<0.05). The scores on aseptic food indicated the importance of various factors, including sanitation (4.86/5.00), good materials (4.82/5.00), nutritional value (4.79/5.00), unused of monosodium glutamate (MSG) (4.66/5.00), taste (4.61/5.00), and reasonable prices (4.60/5.00). The patients who were college graduates showed significantly higher scores and importance in good materials, proper packaging unit, simple cooking methods, food diversity, and familiar foods (p<0.05). In the aseptic menu items, patients wanted to include seasoned raw vegetables (4.11/5.00), a set menu (including cooked grain, soup, and a side dish) (4.08/5.00), seasoned cooked vegetables (4.04/5.00), low sodium kimchi (3.98/5.00), and fermented sauces (3.92/5.00). The patients with gastrointestinal cancer and other cancers (e.g. breast, thyroid, and renal cancer) had significantly higher necessity scores than lung patients for stew, seasoned raw vegetables, salad, low sodium kimchi, and a set menu (p<0.05).

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.20 no.3
• /
• pp.191-201
• /
• 1987

Seasoned sardine meat was prepared to extend the use of sardine for human consumption, and processing conditions and storage stability of frozen seasoned sardine meat were studied during storage at $-20^{\circ}C$. The fish was beheaded, gutted and cleaned in a washing tank. The washed fish was then put through a belt-drum type meat separator which separates the flesh iron the bone and skin. Mechanically deboned fish meat was mixed with $20.6\%$ emulsion curd, $0.5\%$ table salt, $2.0\%$ sugar, $0.4\%$ sodium bicarbonate, $0.2\%$ polyphosphate, $0.1\%$ monosodium glutamate, $0.3\%$ onion powder, $0.1\%$ garlic powder, $0.1\%$ ginger powder, $3.0\%$ soybean protein and $0.1\%$. In sodium erythorbate. This seasoned sardine meat was frozen with contact freezer, packed in a carton box and then stored at $-20^{\circ}C$. The pH, volatile basic nitrogen, viable cell counts, peroxide value, carbonyl value, thiobarbituric acid value, taste compounds, fatty acid composition, salt extractable nitrogen, drip, texture, and color values of the products were determined during frozen storage. The results showed that lipid content in products could be controlled by using emulsion curd, and flavor and texture could be improved by adding spices and soybean protein, and lipid oxidation could be retarded by $0.1\%$ sodium erythorbate. Judging from the results of chemical experiments and sensory evaluation, the products can be preserved in a good quality for 120 days during frozen storage.