• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.515-524
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• 2020

Objective: Human mesenchymal stromal cells (MSCs) exhibit variable differentiation potential and can be divided accordingly into distinct subpopulations whose ratios vary with donor age. However, it is unknown whether the same is true in pigs. This study investigated MSC subpopulations in miniature pig and compared their characteristics in young (2 to 3 months) and adult (27 to 35 months) pigs. Methods: Osteogenic, chondrogenic, and adipogenic capacity of isolated MSCs was evaluated by von Kossa, Alcian blue, and oil red O staining, respectively. Cell surface antigen expression was determined by flow cytometry. Proliferative capacity was assessed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Expression of marker genes was detected by quantitative real-time polymerase chain reaction. Results: Porcine MSCs comprised cells with trilineage and bilineage differentiation potential (tMSCs and bMSCs, respectively) and non-differentiating stromal cells (NDSCs). The tMSC and bMSC fractions were smaller in adult than in young pigs (63.0% vs 71.2% and 11.6% vs 24.0%, respectively, p<0.05); NDSCs showed the opposite trend (25.4% vs 4.8%; p<0.05). Subpopulations showed no differences in morphology, cell surface antigen expression, or proliferative capacity, but octamer-binding transcription factor 4 (OCT4) expression was higher in tMSCs than in bMSCs and NDSCs (p<0.05), whereas sex determining region Y-box 2 (SOX2) expression was higher in tMSCs and bMSCs than in NDSCs (p<0.05). Aging had no effect on these trends. Conclusion: Porcine MSCs comprise distinct subpopulations that differ in their differentiation potential and OCT4 and SOX2 expression. Aging does not affect the characteristics of each subpopulation but alters their ratios.

• The Journal of Korean Association of Computer Education
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• v.13 no.6
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• pp.9-21
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• 2010

Concurrent processes come into conflict with each other when they are competing for the use of the same resources. In the case of competing processes three control problems must be faced: mutual exclusion, deadlock, and starvation. The concurrency is a subject rather difficult to understand. In addition, because concurrent programs included in most of OS texts are described by pseudocode and are not being able to execute directly, almost learners are difficult to understand behaviour of concurrent programs. The purpose of this study is to propose instruction method for mutual exclusion using SDL and MSC base on graphic. In order to verify the effectiveness of the proposed materials, we compare with materials based on pseudocode. The results indicated that the proposed materials is more effective than materials based on pseudocode in teaching-learning for mutual exclusion mechanisms.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.355-359
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• 2006

This study was carried out to investigate the factors affecting seed germination of Korean native orchids, such as Habenaria radiata, Bletilla striate and Cypripedium macranthum. In general, seeds of H. radiata was effectively germinated on solid medium and the highest germination (29.6%) was achieved on Hyponex solid medium. But shoot growth was better in MS liquid medium containing $100mL\;L^{-1}$ coconut water (MSC medium). Seed germination of B. striata was effectively induced on MS solid medium. However, no seed germination was observed in all liguid medium tested. Seed germination experiments of C. macranthum were performed using 6 kinds of media and seeds harvested 90 days after cross- and self-pollination. Cross-pollinated seeds were germinated on all the media with the best germination ratio of 4.45% on MSC medium. But no germination was observed from self-pollinated seeds.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.4
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• pp.588-595
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• 2013

Mesenchymal stem cells (MSCs) are often known to have a therapeutic potential in the cell-mediated repair for fatal or incurable diseases. In this study, canine umbilical cord MSCs (cUC-MSCs) were isolated from umbilical cord matrix (n = 3) and subjected to proliferative culture for 5 consecutive passages. The cells at each passage were characterized for multipotent MSC properties such as proliferation kinetics, expression patterns of MSC surface markers and self-renewal associated markers, and chondrogenic differentiation. In results, the proliferation of the cells as determined by the cumulative population doubling level was observed at its peak on passage 3 and stopped after passage 5, whereas cell doubling time dramatically increased after passage 4. Expression of MSC surface markers (CD44, CD54, CD61, CD80, CD90 and Flk-1), molecule (HMGA2) and pluripotent markers (sox2, nanog) associated with self-renewal was negatively correlated with the number of passages. However, MSC surface marker (CD105) and pluripotent marker (Oct3/4) decreased with increasing the number of subpassage. cUC-MSCs at passage 1 to 5 underwent chondrogenesis under specific culture conditions, but percentage of chondrogenic differentiation decreased with increasing the number of subpassage. Collectively, the present study suggested that sequential subpassage could affect multipotent properties of cUC-MSCs and needs to be addressed before clinical applications.

• Journal of KIISE:Computing Practices and Letters
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• v.6 no.2
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• pp.187-195
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• 2000

Embedded software generates its outputs using current states of the system as well as external inputs. When a module in embedded software is tested, we need an automated testing tool, which generates possible sequences to reach the module as well as input data of the module, to reduce the testing time and to improve the quality of software. In this paper, we use decision table to specify the functionality of the module and data-annotated MSC (Message Sequence Charts) to describe scenarios, and implement a tool, which we call MSCTest, to automate the testing process. MSCTest consists of MSC graphic editor, test sequence and data generator, and test driver generator. MSCTest is effectively applied to test EsWin which is a kind of window library used in embedded systems.

• Journal of Embryo Transfer
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• v.27 no.3
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• pp.175-181
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• 2012

The synovial tissues are a valuable MSCs source for cartilage tissue engineering because these cells are easily obtainable by the intra-articular biopsy during diagnosis. In this study, we isolated and characterized the canine MSCs derived from synovial fluid of female and male donors. Synovial fluid was flushed with saline solution from pre and post-puberty male (cM1-sMSC and cM2-sMSC) and female (cF1-sMSC and cF2-sMSC) dogs, and cells were isolated and cultured in advanced-DMEM (A-DMEM) supplemented with 10% FBS in a humidified 5% $CO_2$ atmosphere at $38.5^{\circ}C$. The cells were evaluated for the expression of the early transcriptional factors, such as Oct3/4, Nanog and Sox2 by RT-PCR. The cells were induced under conditions conductive for adipogenic, osteogenic, and chondrogenic lineages, then evaluated by specific staining (Oil red O, von Kossa, and Alcian Blue staining, respectively) and analyzed for lineage specific markers by RT-PCR. All cell types were positive for alkaline phosphatase (AP) activity and early transcriptional factors (Oct3/4 and Sox2) were also positively detected. However, Nanog were not positively detected in all cells. Further, these MSCs were observed to differentiate into mesenchymal lineages, such as adipocytes (Oil red O staining), osteocytes (von Kossa staining), and chondrocytes (Alcian Blue staining) by cell specific staining. Lineage-specific genes (osteocyte; osteonectin and Runx2, adipocytes; PRAR-${\gamma}2$, FABP and LEP, and chondrocytes; collagen type-2 and Sox9) were also detected in all cells. In this study, we successfully established synovial fluid derived mesenchymal stem cells from female and male dogs, and determined their basic biological properties and differentiation ability. These results suggested that synovial fluid is a valuable stem cell source for cartilage regeneration therapy, and it is easily accessible from osteoarthritic knee.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.6
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• pp.107-115
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• 2002

A parametric study has been conducted to evaluate the effects of finite element modeling methods on the internal loads, sizing and the weight of the multi-spar aircraft wing structures. The wing is idealized into total 18finite element models and subjected to 4typical external load conditions. An automatic sizing algorithm based on MSC/NASTRAN and MSC/PATRAN is developed. The results show that the critical part affection the internal loads and weight of the structure is wing skin. Effect of modeling of the spar and rib on the structural behavior is not manifest. On the contrast to the general expectation, the models using the bending-resistant elements show the heavier weight than ones by the elements without bending stiffness. From this results, designers of multi-spar wing are recommended to construct the finite element model considering the bending stiffness, or to check the characteristics of the structure before modeling.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.35 no.3
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• pp.196-203
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• 2007

The postbuckling behavior and failure of composite cylinders subjected to external hydrostatic pressure are investigated by a finite element method and test. A nonlinear finite element program, ACOS, is used for the postbuckling progressive failure analysis of composite cylinders. A total of 5 carbon/epoxy composite cylinders were fabricated and tested to verify the finite element results. For comparison, analyses by MSC/NASTRAN and MSC/MARC are additionally conducted. Among the softwares, the finite element program, ACOS, predicts the buckling loads the best with about 11 to 26% deviation from experimental results except for one specimen. While the finite element analysis shows global buckling modes with 4 waves in hoop direction, in the experiments the local buckling appears first and results in the final failure without global buckling.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.36S no.10
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• pp.40-50
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• 1999

The novel network architecture is recently required to accommodate a variety and intelligence of communication services. It is also required to provide customized network functions and to efficiently satisfy a various user's requirements. Accordingly, open network architecture based on ATM transport network features has been mainly studied in communication network. This paper evaluates the VSN(Virtual Switch Network) characteristics in the call processing of IMT-2000 switching system, which is composed of VSN instead of ATM switch network. VSN means switch network which is composed of ATM transport network. As a result, this paper proposes new VSN based ATM-MSC architecture with integrated call and connection control systems. and evaluates call processing delay characteristics using call process procedures. Internal call processing delay is increased approximately 3.5msec than the conventional ATM switching system. The experimental values applied in the analysis condition are the load 0.8, and the 100km distance between CCCPs(Call and Connection Control Processors) and VSNs. It is confirmed that the VSN has the potentiality in the practical implementation.

• International Journal of Oral Biology
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• v.38 no.3
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• pp.111-119
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• 2013

Objective. To investigate the effects of the hypoxia inducible factor-1 (HIF-1) activation-mimicking agent cobalt chloride ($CoCl_2$) on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and elucidate the underlying molecular mechanisms. Study design. The dose and exposure periods for $CoCl_2$ in hMSCs were optimized by cell viability assays. After confirmation of $CoCl_2$-induced HIF-$1{\alpha}$ and vascular endothelial growth factor expression in these cells by RT-PCR, the effects of temporary preconditioning with $CoCl_2$ on hMSC osteogenic differentiation were evaluated by RT-PCR analysis of osteogenic gene expression, an alkaline phosphatase (ALP) activity assay and by alizarin red S staining. Results. Variable $CoCl_2$ dosages (up to $500{\mu}M$) and exposure times (up to 7 days) on hMSC had little effect on hMSC survival. After $CoCl_2$ treatment of hMSCs at $100{\mu}M$ for 24 or 48 hours, followed by culture in osteogenic differentiating media, several osteogenic markers such as Runx-2, osteocalcin and osteopontin, bone sialoprotein mRNA expression level were found to be up-regulated. Moreover, ALP activity was increased in these treated cells in which an accelerated osteogenic capacity was also verified by alizarin red S staining. Conclusions. The osteogenic differentiation potential of hMSCs could be preserved and even enhanced by $CoCl_2$ treatment.