• Food Science and Biotechnology
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• 제16권1호
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• pp.104-109
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• 2007

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect three varieties of genetically modified (GM) canola. The construct-specific primers were used to distinguish the following three varieties of GM canola; GT73, MS8xRF3, and T45, using multiplex PCR. The FatA (fatty acyl-ACP thioesterase) gene was used as an endogenous canola reference gene in the PCR detection. The primer pair Canendo-FIR containing a 105 bp amplicon was used to amplify the FatA gene and no amplified product was observed in any of the 15 different plants used as templates. The GT73-KHUF1/R1 primer recognized the 3'-flanking region of GT73, resulting in an amplicon of 125 bp. The Barstar-F1/MS8xRF3-R primer recognized the junction region of bars tar and the NOS terminator introduced into MS8xRF3, resulting in a 162 bp amplicon, and the T45-F2/R2 primer recognized the junction region of PAT and the 35S terminator introduced into T45, resulting in an amplicon of 186 bp. This multiplex PCR allowed for the detection of construct-specific targets in a genomic DNA mixture of up to 1% GM canola containing GT73, MS8xRF3, and T45.

• 대한전자공학회논문지SD
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• 제38권8호
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• pp.13-19
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• 2001

분자선 결정 성장법을 이용하여 성장된 서로 다른 장벽층의 두께를 갖는 $Al_{0.3}Ga_{0.7}N$ heterojunction field effect transistors (HFETs) 를 제작하여 그 특성을 비교, 관찰하였다. $Al_{0.3}Ga_{0.7}N$층의 두께에 따른 특성의 비교로부터 최적의 2 차원 전자개스 (2 dimensional electron gas) 를 가질 수 있는 $Al_{0.3}Ga_{0.7}N$/GaN HFET 소자 구조를 얻을 수 있었다. $L_g=0.6$ ${\mu}m$ 와 $W_g=34\;{\mu}m$ $Al_{0.3}Ga_{0.7}N$/GaN (20 nm/2 mm) HFET에서 Imax ($V_{gs}=1\;V$) 와 $G_{m,maX}$는 각각 1.155 A/mm 및 250ms/${\mu}m$ 이었으며 $F_t=13$ GHz 와 $F_{max}=48$ GHz의 우수한 고주파 특성을 얻을 수 있었고 2 inch 기판상에서 제작된 소자들은 5% 이하의 매우 균일한 DC 특성을 나타내었다. 이와 더불어 게이트-드레인 간의 간격에 따른 소자의 특성을 관찰함으로서 소자의 항복전압과 고주파 특성과의 상관관계를 고찰하였다.

• 한국자원식물학회지
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• 제31권3호
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• pp.228-236
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• 2018

In this study, we evaluated the antioxidant activity and protective effects against oxidative DNA damage of the ethyl acetate fraction from the callus of Abeliophyllum distichum Nakai (ECA). Callus of A. distichum was induced on MS medium containing NAA (1 mg/L) and 2,4-D (1 mg/L), and a sufficient amount was obtained for the extraction by subculture. Acteoside was analyzed and quantified (0.39 mg/g callus) from ECA using the high-performance liquid chromatography-photodiode array detector method. ECA showed very high antioxidative activity as revealed by DPPH and ABTS scavenging assays. The $IC_{50}$ values were 12.4 and $6.8{\mu}g/ml$, respectively. ECA showed protective effects against oxidative DNA damage evaluated by using ${\Psi}X-174$ RF I plasmid DNA. It also inhibited DNA damage by suppressing the oxidative stress-induced protein and mRNA levels of ${\gamma}$-H2AX and p53 in NIH/3T3 cells. In conclusion, ECA protects against oxidative DNA damage through its powerful antioxidant activity.