• Journal of Wetlands Research
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• v.21 no.1
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• pp.34-42
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• 2019

Baseflow which is one of the unmeasurable components of streamflow and slowly flows through underground is important for water resource management. Despite various separation methods from researches preceded, it is difficult to find a significant separation method for baseflow separation. This study applied the MRC method and developed the improved approach to separate baseflow from total streamflow hydrograph. Previous researchers utilized the whole streamflow data of study period at once to derive synthetic MRCs causing unreliable results. This study has been proceeded with total nine areas with gauging stations. Each three areas are selected from 3 domestic major watersheds. Tool for drawing MRC had been used to draw MRCs of each area. First, synthetic MRC for whole period and two other MRCs were drawn following two different criteria. Two criteria were set by different conditions, one is flow condition and the other is seasonality. The whole streamflow was classified according to seasonality and flow conditions, and MRCs had been drawn with a specialized program. The MRCs for flow conditions had low R2 and similar trend to recession segments. On the other hand, the seasonal MRCs were eligible for the baseflow separation that properly reflects the seasonal variability of baseflow. Comparing two methods of assuming MRC for baseflow separation, seasonal MRC was more effective for relieving overestimating tendency of synthetic MRC. Flow condition MRCs had a large distribution of the flow and this means accurate MRC could not be found. Baseflow separation using seasonal MRC is showing more reliability than the other one, however if certain technique added up to the flow condition MRC method to stabilize distribution of the streamflow, the flow conditions method could secure reliability as much as seasonal MRC method.

• Journal of Chest Surgery
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• v.29 no.11
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• pp.1173-1181
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• 1996

The causes of degenerative changes in allograft cardiac valves are not well known to this day. Today's preserved allografts possess highly viable endothelial cells and degeneration of allografts can be facilitated by immune reaction which may be mediated by these viable cells. To test the antigenicity of endothelial cells, pieces from aortic wall were obtained from fresh and cryo-preserved rat allograft. Timings of sampling were prior to sterilization, after sterilization, after 1, 2, 7, 14 days of fresh preservation and cryopreservation. Endothelial cells were tested by immunohistochemical methods using monoclonal antibodies to MHC class I(MRC OX-18), class II(MRC OX-6) and ICAM-1 antigens. After transplantation of each group of aortic allograft at the subcutaneous layers of rats, population of CD4$^{+}$ T cell and CD8$^{+}$ T cell were analyzed with monoclonal antibodies after 1, 2, 3, 4, 6 and 8 weeks. MHC class I expression was 23.95% before preservation and increased to 35.53~48.08% after preservation(p=0.0183). MHC Class II expression was 9.72% before preservation and 10.13~13.39% after preservation(P=0.1599). ICAM-1 expression was 15.02% before preservation and increased to 19.85~35.33% after preservation(P=0.001). The proportion of CD4$^{+}$ T-cell was 42.13% before transplantation. And this was 49.23~36.8% after transplantation in No treat group (p=0.955), decreased to 29.56~32.80% in other group(p=0.0001~0.008). In all the groups, the proportion of CD8$^{+}$ T-cell increased from 25.57% before transplantation to 42.32~58.92% after transplantation(p=0.000l~0.0002). The CD4$^{+}$/CD8$^{+}$ ratio decreased from 1.22~2.28 at first week to 0.47~0.95 at eighth week(p=0.0001). The results revealed that the expression of MHC class I and ICAM-1 in aortic allograft endothelium were increased but that of MHC class II were not changed, despite the different method of preservation. During 8 weeks after transplantation of aortic allograft, the subpopulations of CD4$^{+}$ T cell were not changed or only slightly decreased but those of CD8$^{+}$ T cell were progressively increased.ely increased.