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Development of Miniaturized Automatic Chromatography System for Column Performance Validation : MiniValChrom

  • Park, Jae-Ha;Lee, Eun-Gyu
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.147-150
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    • 2000
  • Bioprocess chromatography is probably the most widely used unit operation in biopharmaceutics manufacturing. To obtain a good quality product reproducibly, the chromatography process validation should be performed carefully. We developed a miniaturized automatic chromatography system, MiniValChrom, for column performance validation.

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THE REGULATION OF CYP1A1 GENE EXPRESSION BY ESTRADIOL AND ITS METABOLITES

  • Joung, Ki-Eun;Sheen, Yhun-Yhong
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.11b
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    • pp.149-150
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    • 2002
  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most potent halogenated aromatic hydrocarbon congener that induces expression of several genes including CYP1A. Exposure to TCDD results in many toxic actions such as carcinogenesis, hepatotoxicity, immune suppression, reproductive toxicity and developmental toxicity.(omitted)

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Study on Optimization of Detection System of Prompt Gamma Distribution for Proton Dose Verification (양성자 선량 분포 검증을 위한 즉발감마선 분포측정 장치 최적화 연구)

  • Lee, Han Rim;Min, Chul Hee;Park, Jong Hoon;Kim, Seong Hoon;Kim, Chan Hyeong
    • Progress in Medical Physics
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    • v.23 no.3
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    • pp.162-168
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    • 2012
  • In proton therapy, in vivo dose verification is one of the most important parts to fully utilize characteristics of proton dose distribution concentrating high dose with steep gradient and guarantee the patient safety. Currently, in order to image the proton dose distribution, a prompt gamma distribution detection system, which consists of an array of multiple CsI(Tl) scintillation detectors in the vertical direction, a collimator, and a multi-channel DAQ system is under development. In the present study, the optimal design of prompt gamma distribution detection system was studied by Monte Carlo simulations using the MCNPX code. For effective measurement of high-energy prompt gammas with enough imaging resolution, the dimensions of the CsI(Tl) scintillator was determined to be $6{\times}6{\times}50mm^3$. In order to maximize the detection efficiency for prompt gammas while minimizing the contribution of background gammas generated by neutron captures, the hole size and the length of the collimator were optimized as $6{\times}6mm^2$ and 150 mm, respectively. Finally, the performance of the detection system optimized in the present study was predicted by Monte Carlo simulations for a 150 MeV proton beam. Our result shows that the detection system in the optimal dimensions can effectively measure the 2D prompt gamma distribution and determine the beam range within 1 mm errors for 150 MeV proton beam.

Protein Evaluation of Dry Roasted Whole Faba Bean (Vicia faba) and Lupin Seeds (Lupinus albus) by the New Dutch Protein Evaluation System: the DVE/OEB System

  • Yu, P.;Egan, A.R.;Leury, B.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.6
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    • pp.871-880
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    • 1999
  • The effects of dry roasting (110, 130, $150^{\circ}C$ for 15, 30, 45 min) on potential ruminant protein nutritional values in terms of: a), rumen bypass protein (BCP); b), rumen bypass starch (BST); c), fermented organic matter (FOM); d), true absorbed bypass protein (ABCP); e) microbial protein synthesized in the rumen based on available energy (E_MP); f), microbial protein synthesized in the rumen based on available nitrogen (N_MP); g), true protein supplied to the small intestine (TPSI); h), true absorbed rumen synthesized microbial protein (AMP); i), endogenous protein losses (ENDP); j), true digested protein in the small intestine (DVE); k), degraded protein balance (OEB) of whole lupin seeds (WLS) and faba beans (WFB) were evaluated by the new Dutch DV/OEB protein evaluation system. Dry roasting significantly increased BCP, BST, TPSI, ABCP, DVE (p<0.001) and decreased FOM, E_MP, AMP, N_MP and OEB (p<0.001) with increasing temperatures and times except that when temperature was at $110^{\circ}C$. The values of BCP, BST, TPSI, ABCP and DVE at $150^{\circ}C/45min$ for WLS and WFB were increased 2.2, 3.7; -, 2.0; 1.7, 1.7; 2.3, 3.7 and 1.7, 1.7 times and the values of FOM, E_MP, AMP, N_MP and OEB at $150^{\circ}C/45min$ for WLS and WFB were decreased by 15.3, 25.8; 18.1, 25.8; 18.7, 25.8; 54.6, 41.6 and 82.3% 54.7%, respectively, over the raw WLS and WFB. The results indicated that though dry roasting reduced microbial protein synthesis due to reducing FOM, TPSI didn't decrease but highly increased due to increasing BCP more than enough for compensation of the microbial protein decreasing. Therefore the net absorbable DVE in the small intestine was highly increased. The OEB values were significantly reduced for both WLS and WFB but not to the level of negative. It indicated that microbial protein synthesis might not be impaired due to the sufficient N supplied in the rumen, but the high positive OEB values in the most treatments except of $150^{\circ}C$ for 30 and 45 min of WLS (The OEB values: 54.8 and 26.0 g/kg DM) indicated that there were the large amounts of N loss in the rumen. It was concluded that dry roasting at high temperature was effective in shifting protein degradation from rumen to intestines and it increased the DVE values without reaching the negative OEB values. No optimal treatment was found in WLS due to the too high OEB values in all treatments. But dry roasting at $150^{\circ}C$ for 30 and 45 min might be optimal treatments for WLS due to the very lower OEB values.

Effect of Biodegradable Film Mulching on Soil Environment and Onion Growth and Yield (생분해성 멀칭필름이 토양환경과 양파 생육 및 수량에 미치는 영향)

  • Ji-Sik Jung;Do-Won Park;Hyun-Sug Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.207-215
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    • 2023
  • This study was compared the soil environment and growth and yield of onion (Allium cepa L.) treated with non-mulching (NM) and mulching polyethylene film (PEF) and two biodegradable films (BFI and BFII) commonly used in farmhouses. Visual observation confirmed the degradation of BFI and BFII films after 150 days after tansplanting (DAT). BFII increased light penetration into the films and reduced the weight maintenace after 180 DAT, with a high decompostion at 30 days after soil tilling. Soil moisture contents much fluctuated between -14 kP and - 0 kPa in NM plots, increasing the minimum soil temperature of BFI plots. Mulching treatments decreased soil organic matter contents but did not subtantially increase soil mineral nutrients, soil bulk density, and number of bacteria compared to those of NM plots. Onion root growth was increased by PEF and BFI treatments at an early growth stage, 60 DAT, with the most remarkable stem extension observed for PEF and BFI treatments after 150 DAT. PEF and BFI treatments increased the bulb's diameter, length, weight, and lodging at 180 DAT. BFI treatments exhibited a high portion of the "very large" category producing with 55.3 tons ha-1 based on the classification into bulb size, followed by PE (49.3 tons), NM (9.4 tons), and BFII treatments (2.7 tons) at 230 DAT.

Analysis of Awareness of Teachers for Core Competencies and Scientific Core Competencies (핵심역량과 과학과 교과역량에 대한 초등 교사의 인식 분석)

  • Ha, Ji-hoon;Shin, Youngjoon
    • Journal of Korean Elementary Science Education
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    • v.35 no.4
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    • pp.426-441
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    • 2016
  • The purpose of this study was getting the information for successful application to the national curriculum and students' core competencies enhancement, through investigation about competencies discussed in 2015 revised national curriculum development process and analysis about perception of 150 elementary school teachers in study. The results were as follows : Communication skill is considered to be the most important. Thinking ability what has been important traditionally is the middle of the rankings. Elementary school teachers think that a competency is specific to a subject. From this point of view, Creative/Scientific Problem-Solving Ability is the most important in science. They think that the enhancing of the ability of inquiry performance is highlighted in current science class. On elementary school teachers' awareness, inquiry model is the most effective in enhancing of scientific thinking and the ability of inquiry performance. And STS instruction model is in the other. PBL learning model and experimental inquiry model is the most effective in enhancing a competency has the highest feasibility like scientific thinking or the ability of inquiry performance.

A Study on the Wearing Pattern and Design Preference of Handbag for Career Women (직업여성의 핸드백 착용실태와 디자인 선호도 분석)

  • Koo, In-Sook
    • Journal of Fashion Business
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    • v.12 no.4
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    • pp.1-14
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    • 2008
  • The domestic handbag market has potential of growth - the key to protect this growing market from foreign makers is good design and better quality. 68 people(29.6%)out of 230 survey participants report 'to own 5 handbags', and the most popular(frequently reported) maker is 'MCM', owned by 49 participants(21.3%). 150 participants(65.2%) out of 230 total participants purchase at least one handbag a year, and 136 participants(59.7%) out of 230 spend between 110,000 and 200,000 Korean wons. 52 participants(22.6%) out of 230 own high-end handbags worth 500,000 Korean won or more. Among the high-end handbags, Louis Vuitton is the most popular, owned by 19 participants. The overall criteria in handbag purchase are 'color', 'price', 'pattern and logo', 'reputation', 'material', 'storage room', and 'size', in the descending order of importance. The most frequently desired benefit in handbag purchase is 'to stay through trends' i.e. not to go out of fashion too easily. The favored designs are made with rectangle shape with large width, soft material, attached handles, leather material, modern style. The most frequently cited reason to purchase a high-end brand item is 'because of high quality'.

A Study on the Relationship between the Sex-role Attitude and Marital Satisfaction -With Special Reference to Urban Wives Seoul. (성역할 태도와 결혼 만족도간의 관계 -도시 부부를 중심으로-)

  • 박태온
    • Journal of Families and Better Life
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    • v.1 no.2
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    • pp.139-150
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    • 1983
  • The purpose of this study was to investigate the relationship between wive's sex-role attitude and their marital satisfaction for understanding a part of marital relations. For this purpose, a questionnaire was constructed and administrated to 626 married women. the sex role attitude scale was a modified version of the sex-role attitude scale constructed by Osmond and Martin, and the marital satisfaction scale was developed basing upon the scales used by Terman and Hayes & Stinnett. The result of this study are summarized as follows; The majority of wives have relatively modem attitude toward their sex role in the extra familial roles. Wives have relatively modem attitude toward their sex role when the couples are younger, more educated, on a higher income level and working or a job (especially or a professional job). Wife's education level, husband's education level and family income make significant difference in their marital satisfaction specifically, a wife would be relatively more satisfied when she has more education and the family income level is higher. It is founded that wives sex role attitude has significant relationship with their marital satisfaction. A wife tends to be more satisfied when she has a traditional sex role attitude . A professional wife who has a traditional attitude shows most satisfaction and one who has a modern attitude and not having a job tends to be most dissatisfied with her marriage. It is also founded that a wife who has traditional attitude and received college education are above shows most satisfaction while the wife who has modern attitude and received high school education or below shows most dissatisfaction with their marriage lives.

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A Study on School Uniform and Ornaments Behaviors of High School Girls (여고생의 교복 및 장식 행동에 관한 연구 -경상남도 마산.창원 지역을 중심으로-)

  • 정혜경;김세희
    • The Research Journal of the Costume Culture
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    • v.12 no.1
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    • pp.136-150
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    • 2004
  • The purpose of this study was to examine how high school girl students used their school uniforms and what types of ornaments they preferred, in an effort to identify the image of school uniform. The result of this study were as follow: First, 52.6 percent of the students investigated altered their uniforms, and as fur the major alteration style, revival one to take them in to be well-fitting was dominant. But concerning skirts, hip-hop style was also found. The students viewed ornaments more favorably, as the larger group of the students perceived that it was necessary to restrict the school-uniform alteration yet not to control ornaments. Concerning how much they wore school uniforms, most of them continued to wear them outside school in many cases. Specifically, those who engaged in pastime or went shopping wearing school uniforms enjoyed altering them, and they also enjoyed using ornaments that weren't allowed by the school dress code. Second, as to school-uniform image, elegant image was most preferred, followed by practical, innocent and fashion-conscious ones. They were most preferred with its elegant image. Third, as to ornaments image, they were most preferred with its positive image.

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Product Quality and Shelf-life Effect of Low-fat Functional Sausages Manufactured with Sodium Lactate and Chitosans During Storage at 15℃ (젖산나트륨과 키토산을 첨가한 저지방 기능성 소시지의 15℃에서 저장 중 품질 및 저장성 효과)

  • Chin, Koo-Bok;Kook, Sung-H.;Choi, Soon-H.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.655-666
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    • 2005
  • This study was performed to measure physicochemical and textural characteristics, and shelf-life effect of low-fat functional sausages(LFFSs) manufactured with sodium lactate(SL, 3.3%), lac pigment and various molecular weights(MWs) of chitosan (Low=1.5 kDa, Med=30-50 kDa and High=200 kDa) during storage at 15$^{\circ}C$ for 18 days. LFFSs had 73.7-76.0% moisture, lower than 3% fat and 14-15% protein, respectively. pH values were 6.05-6.44 and the control(150 ppm, $NaNO_2$) was the lowest among LFFSs (p<0.05). Increasing storage time decreased pH values, but no differences in pH values were observed up to 6 days of storage (p>0.05). LFFSs containing SL and low MW of chitosan improved water holding capacity (WHC) and different from those with SL and medium-MW chitosan. WHC was decreased with increased storage time and differences of WHC were observed from 18 days of storage. The addition of chitosan reduced both lightness and redness values, as compared to 150 ppm sodium nitrite(SN), and increased storage time decreased yellowness(p<0.05), especially at 12 days of storage. LFFSs with SL and medium-MW chitosan increased most textural properties compared to the control(p<0.05). The addition of SN of 150 ppm in LFFSs retarded microbial growth for E. coli 0157:H7, while those with SL tended to have an antimicrobial effect for Listeria monocytogenes(LM). The growth rate of LM was delayed by addition of various MW of chitosans in LFFSs, especially high MW chitosan, as compared to LFFSs containing SL alone. These results indicated that the functional, textural and antimicrobial effects of LFFSs were improved by addition SL and various MW of chitosan combinations. In addition, 0.05% lac pigment improved the cure color of LFFSs similar to those of 150 ppm SN.