• Nuclear Medicine and Molecular Imaging
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• v.41 no.3
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• pp.226-233
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• 2007

Purpose: Dual reporter gene imaging has several advantages for more sophisticated molecular imaging studies such as gene therapy monitoring. Herein, we have constructed hepatoma cell line expressing dual reporter genes of sodium iodide symporter (NIS) and enhanced green fluorescence protein (EGFP), and the functionalities of the genes were evaluated in vivo by nuclear and optical imaging. Materials and Methods: A pRetro-PN vector was constructed after separating NIS gene from pcDNA-NIS. RSV-EGFP-WPRE fragment separated from pLNRGW was cloned into pRetro-PN vector. The final vector expressing dual reporter genes was named pRetro-PNRGW. A human hepatoma (HepG2) cells were transfected by the retrovirus containing NIS and EGFP gene (HepG2-NE). Expression of NIS gene was confirmed by RT-PCR, radioiodine uptake and efflux studies. Expression of EGFP was confirmed by RT-PCR and fluorescence microscope. The HepG2 and HepG2-NE cells were implanted in shoulder and hindlimb of nude mice, then fluorescence image, gamma camera image and I-124 microPET image were undertaken. Results: The HepG2-NE cell was successfully constructed. RT-PCR showed NIS and EGFP mRNA expression. About 50% of cells showed fluorescence. The iodine uptake of NIS-expressed cells was about 9 times higher than control. In efflux study, $T_{1/2}$ of HepG2-NE cells was 9 min. HepG2-NE xenograft showed high signal-to-background fluorescent spots and higher iodine-uptake compared to those of HepG2 xenograft. Conclusion: A hepatoma cell line expressing NIS and EGFP dual reporter genes was successfully constructed and could be used as a potential either by therapeutic gene or imaging reporter gene.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.49 no.3
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• pp.30-39
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• 2012

In this paper, a safe driving assistance system is proposed by detecting the status of driver's doze based on face and eye detection. By the level of the fatigue, safe driving system alarms or set the seatbelt on vibration. To reduce the effect of backward light and too strong solar light which cause a decrease of face and eye detection rate and false fatigue detection, post processing techniques like image equalization are used. Haar transform and PCA are used for face detection. By using the statistic of the face and eye structural ratio of normal Koreans, we can reduce the eye candidate area in the face, which results in reduction of the computational load. We also propose a new eye status detection algorithm based on Hough transform and eye width-height ratio, which are used to detect eye's blinking status which decides doze level by measuring the blinking period. The system alarms and operates seatbelt on vibration through controller area network(CAN) when the driver's doze level is detected. In this paper, four algorithms are implemented and proposed algorithm is made based on the probability model and we achieves 84.88% of correct detection rate through indoor and in-car environment experiments. And also we achieves 69.81% of detection rate which is better result than that of other algorithms using IR camera.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.11 no.5
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• pp.136-146
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• 2012

As information technology used for simulation and virtual reality developed, there is a growing interest in animation technologies which will effectively deliver simulation results to users. Various efforts have been made to improve animation performance, like playback quality and speed, input-output speed and storage space reduction. However, earlier studies generally focused on image compression frame by frame. To significantly improve storage space and playback speed, animation data should be vectorized. Also, spatial and temporal duplication have to be removed. In this study, animation data structure was improved fundamentally through establishment of hierarchy and vectorization. Also Spatial and temporal duplication of animation data was removed through vectorization based on train route. As a result, storage space was reduced, input-output speed and playback speed were considerably improved. According to the test, additional Patternization which followed vectorization brought reduction of over 80% in storage space and input-output speed was quadrupled. Patternization technology can be used as a proper storage method of animation data, and can provide user-specific animation by small data transmission.

• The Journal of the Acoustical Society of Korea
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• v.39 no.1
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• pp.16-23
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• 2020

In High-Intensity Focused Ultrasound (HIFU) treatment, effective localization of HIFU focus is important for developing a safe treatment plan. While Magnetic Resonance Imaging guided HIFU (MRIgHIFU) can visualize the ultrasound path during the treatment for localizing HIFU focus, it is challenging in ultrasound imaging guided HIFU (USIgHIFU). In the present study, a real-time ultrasound beam visualization technique capable of localizing HIFU focus is presented for USIgHIFU. In the proposed method, a short pulse, with the same center frequency of an imaging ultrasound transducer below the regulated acoustic intensity (i.e., Ispta < 720 mW/㎠), was transmitted through a HIFU transducer whereupon backscattered signals were received by the imaging transducer. To visualize the HIFU beam path, the backscattered signals underwent dynamic receive focusing and subsequent echo processing. From in vitro experiments with bovine serum albumin gel phantoms, the HIFU beam path was clearly depicted with low acoustic intensity (i.e., Ispta of 94.8 mW/㎠) and the HIFU focus was successfully localized before any damages were produced. This result indicates that the proposed ultrasound beam path visualization method can be used for localizing the HIFU focus in real time while minimizing unwanted tissue damage in USIgHIFU treatment.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.147-147
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• 2017

우리나라에서는 수입 개방화 추세에 따라 공정한 유통질서 확립하고 국내 생산자와 소비자를 보호하기 위하여 원산지 표시제가 시행되고 있다. 그러나 수입 농산물과 국산 농산물의 큰 가격차이로 인하여 원산지를 허위 표시하는 경우가 증가하고 있다. 특히 쌀 관세화 전환 의무에 따른 수입산 쌀 증가하고 있으며 단립종과 중립종 수입산 쌀은 국내산 쌀과 외관이 유사하여 육안식별이 어려워 국내산 쌀로 둔갑할 우려가 있다. 이에 신속하고 비파괴적으로 쌀의 원산지를 판별할 수 있는 기술 개발이 요구되고 있다. 따라서 본 연구에서는 국내산 쌀과 중국산 쌀의 원산지를 신속하게 판별가능한 영상 처리기술을 개발하였다. 쌀은 국내에서 생산된 중립종 50점과 중국에서 생산된 단립종 및 중립종 51점이 수집되어 사용되었다. 쌀의 분광 영상은 초분광 가시광 및 근적외선 영상 시스템을 이용하여 측정하였다. 이 시스템은 할로겐-텅스텐 라인광, 시료 이송부, 초분광 영상 획득부로 구성되어 있다. 텅스텐-할로겐 라인광은 $15^{\circ}$ 각도로 대칭으로 시료에 조사되고 400 ~ 1000 nm 파장 영역의 반사광 영상 스펙트럼이 측정되었다. 초분광 영상 데이터는 광에 노출되지 않은 암실에서의 파장별 영상과 반사율이 99% 이상인 기준판의 파장별 영상을 이용하여 교정되었다. 부분최소제곱회귀법을 이용하여 쌀 원산지 판별모델 식을 개발하였고, 이 판별 모델식을 교정된 초분광 영상에 적용하여 영상처리 판별 모델을 개발하였다. 그 결과, 원산지 판별정확도가 97.4% 이상으로 나타났으며, 국내산과 중국산 쌀의 원산지 판별이 가능하였다.

• Geophysics and Geophysical Exploration
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• v.2 no.1
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• pp.8-16
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• 1999

In terms of high resolution, seismic reflection survey is by far the most significant geophysical method applied to define subsurface structure. In shallow seismic reflection survey, it is, however, difficult to obtain high resolution image due to both the wave attenuation in the unconsolidated layer and the existence of source-generated surface waves Therefore, when collecting data, it is imperative to select proper equipments and choose optimum field data acquisition parameters for acquiring high S/N data. In this survey, a small size hammer was used as a low energy source and 40-Hz vertical geophones were used as receivers. Trigger signal was obtained from the hammer starter attached in the aluminum plate and thus it was possible to control the source onset time for the vertical stack. During the field work, a modified standard CMP technique was introduced to achieve the many-fold CMP data effectively. Data processing was conducted by the 'Seismic Unix' which is mounted on PC with a Linux operating system. The main distinctions were the emphasis and detail placed on near-surface velocity analysis and the extra care exercised in muting.

• Research in Community and Public Health Nursing
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• v.13 no.1
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• pp.161-170
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• 2002

Purpose: To evaluate community health nursing practice by nursing students for basic data to improve nursing practice education, and to determine the clinical practice experiences of nursing students at public health centers. Method: According to methodological triangulation, all data was collected from March 2001 to June 2001 through prepared questionnaires with 62 Participants, and in depth interviews with 2 Participants, and content analysis with 8 Participants. Results: 1. Clinical practice content was needed to make up professionally. 2. Clinical practice instructor was satisfied with teaching by community health nursing professor, but not by public health center nurse. 3. Clinical environment was perceived as good. 4. Clinical practice time was perceived suitable, but practice evaluation was given a low grade. 5. Experience of nursing students was grouped into 9 categories and 20 themes. 9 Categories are as follows: change of public health center image, difficulty in the personal relationship, positive personal relationships, doubt about the attitude, doubt about the task, tension, worth, ambivalance of the conference, feeling about the end stage of the clinical practice. Twenty themes are stereotypic of the public health care center: recognition, difficulty between tasks and interrelationships with the clients, decrease of self esteem due to deficiency of trust with the students, exchange of feelings in the relationships, the situation of clients was not considered, feelings were disregarded, not systematic, regarding students as supplementory manpower, independent work but not enough contents, anxiety & fear, self-trust, be skilled in the practice, pragmatic knowledge formation, understanding of the old person, self-study of oneself. live up to the expectation, consider as the job, dissolve the stress and impose burden, feeling the lack regarding active approach in the practice. Conclusion: The students have perceived the need of community health nursing through the clinical practice. Clinical practice of community health nursing requires content supplement, correction of instruction by public center nurses, environmental alteration, evaluation criteria set up. Experience of clinical practice in the public health center was as follows: Getting rid of the stereotype about public health center, nursing students comprehended variety and were worth while to do clinical practice in spite of tension& anxiety and fear. And they thought to be a community nurse someday.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.10 no.4
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• pp.77-85
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• 1990

The application of remote sensing in small scale mapping has recently been widened to various fields such as information analysis of landuse, environmental conservation and natural resources. SPOT imagery, in particular, offers data which can be processed for 3-dimensional point determination. This is made possible by its high resolution, appropriate swatch width/altitude ratio and stereo imaging capabilities. This study aims to develop a suitable polymonial and an algorithm in the determination of exterior orientation which is essential in the 3-dimensional point determination of SPOT imgery. An algorithm is presented in this study to determine the exterior orientation of a preprocessed level lB film of the satellite image. It was found that a polynominal of 15 parameters is the best fit polynominal for exterior orientation determination, where 1st order line function is used for positon ($X_o$, $Y_o$, $Z_o$) and 2nd order line function is used for orientation (${\kappa}_o$, ${\phi}_o$, ${\omega}_o$).

• Economic and Environmental Geology
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• v.28 no.3
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• pp.199-211
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• 1995

The deep sea camera system could render it possible to obtain the detailed information of the nodule distribution, but difficult to estimate nodule abundance quantitatively. In order to estimate nodule abundance quantitatively from deep seabed photographs, the nodule abundance equation was derived from the box core data obtained in KODOS area(long.: $154^{\circ}{\sim}151^{\circ}W$, lat.: $9^{\circ}{\sim}12^{\circ}N$) during two survey cruises carried out in 1989 and 1990. The regression equation derived by considering extent of burial of nodule to Handa's equation compensates for the abundance error attributable to partial burial of some nodules by sediments. An average long axis and average extent of burial of nodules in photographed area are determined according to the surface textures of nodules, and nodule coverage is calculated by the image analysis method. Average nodule abundance estimated from seabed photographs by using the equation is approximately 92% of the actual average abundance in KODOS area. The measured sampling points by box core or free fall grab are in general very sparse and hence nodule abundance distribution should be interpolated and extrapolated from measured data to uncharacterized areas. The another goal of this study is to depict continuous distribution of nodule abundance in KODOS area by using PC-version of geostatistical model in which several stages are systematically proceeded. Geostatistics was used to analyse spatial structure and distribution of regionalized variable(nodule abundance) within sets of real data. In order to investigate the spatial structure of nodule abundance in KODOS area, experimental variograms were calculated and fitted to a spherical models in isotropy and anisotropy, respectively. The spherical structure models were used to map out distribution of the nodule abundance for isotropic and anisotropic models by using the kriging method. The result from anisotropic model is much more reliable than one of isotropic model. Distribution map of nodule abundance produced by PC-version of geostatistical model indicates that approximately 40% of KODOS area is considered to be promising area(nodule abundance > $5kg/m^2$) for mining in case of anisotropy.

• Journal of Korean Society for Geospatial Information Science
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• v.7 no.1 s.13
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• pp.53-63
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• 1999

For the close range digital photogrammetry, multi-step procedures should be embodied in an integrated system. However, it is hard to construct an Integrated system through conventional procedural processing. Using Object Oriented Programming(OOP), photogrammetric processings can be classified with corresponding subjects and it is easy to construct an integrated system lot digital photogrammetry as well as to add the newly developed classes. In this study, the equation of 3-dimensional mathematic model is developed to make an immediate calibration of the CCD camera, the focus distance of which varies according to the distance of the object. Classes for the input and output of images are also generated to carry out the close range digital photogrammetric procedures by OOP. Image matching, coordinate transformation, dirct linear transformation and bundle adjustment are performed by producing classes corresponding to each part of data processing. The bundle adjustment, which adds the principle coordinate and focal length term to the non-photogrammetric CCD camera, is found to increase usability of the CCD camera and the accuracy of object positioning. In conclusion, classes and their hierarchies in the digital photogrammetry are designed to manage multi-step procedures using OOP and close range digital photogrammetric process is implemented using CCD camera in an integrated System.