• Molecules and Cells
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• v.21 no.3
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• pp.436-442
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• 2006

Different proliferation of neuroblast 6-4 (NB6-4) in the thorax and abdomen produces segmental specific expression pattern of several neuroblast marker genes. NB6-4 is divided to form four medialmost cell body glia (MM-CBG) per segment in thorax and two MM-CBG per segment in abdomen. As homeotic genes determine the identities of embryonic segments along the A/P axis, we investigated if temporal and specific expression of homeotic genes affects MM-CBG patterns in thorax and abdomen. A Ubx loss-of-function mutation was found to hardly affect MM-CBG formation, whereas abd-A and Abd-B caused the transformation of abdominal MM-CBG to their thoracic counterparts. On the other hand, gain-of-function mutants of Ubx, abd-A and Abd-B genes reduced the number of thoracic MM-CBG, indicating that thoracic MM-CBG resembled abdominal MM-CBG. However, mutations in Polycomb group (PcG) genes, which are negative transregulators of homeotic genes, did not cause the thoracic to abdominal MM-CBG pattern transformation although the number of MM-CBG in a few percent of embryos were partially reduced or abnormally patterned. Our results indicate that temporal and spatial expression of the homeotic genes is important to determine segmental-specificity of NB6-4 daughter cells along the anterior-posterior (A/P) axis.

• The Journal of Korean Orthopaedic Ultrasound Society
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• v.7 no.1
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• pp.1-6
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• 2014

Purpose: In order to know the effectiveness of ultrasonographic examination in diagnosis and follow-up evaluation for the frozen shoulder, we compared those with contralateral side in initial and after steroid injection. Materials and Methods: For the 20 unilateral frozen shoulder from July 2012 to July 2013, ultrasonographic examination was tried using reference line 1 (CBT: distance from coracoid process to LHB tendon) and line 2 (CBG: distance from coracoid process to bicipital groove). We tried 1 time steroid injection and compared the improvement in gross motion and reference line with 2 month, 4 month, and 6 month's serial ultrasonographic examination. Results: The differences on CBT line between lesion side and normal side were -5.6 mm, -5.0 mm, and -4.3 mm in neutral (Neut), external rotation (ER) and internal rotation (IR), respectively. The differences on CBG line were -6.1 mm, -4.7 mm, and -5.0 mm respectively (p<0.05). The changes in the reference line after steroid injection were evaluated at 2 month (CBT: -4.8 mm, -3.5 mm, -2.6 mm / CBG: -4.7 mm, -4.0 mm, -3.6 mm), 4 month (CBT: -4.7 mm, -3.2 mm, -1.7 mm / CBG: -4.3 mm, -3.7 mm, -1.2 mm), and 6 month (CBT: -1.1 mm, -2.9 mm, -0.5 mm / CBG: -1.2 mm, -0.7 mm, -0.9 mm). The gross motion was improved at 4 month after injection, from elevation $108^{\circ}$, ER $32^{\circ}$, IR L5 in initial to $133^{\circ}$, $42^{\circ}$, L3 respectively (p<0.05). Pain improved from 7.5 in initial to 3.0 at 2 month on visual analog scale (VAS). Conclusion: The serial examination after steroid injection revealed that the delayed improvement on reference line (6 month) compared with pain (2 month) or gross motion (4 month). Dynamic ultrasonogram was useful in diagnosis and follow-up evaluation of frozen shoulder.

• Journal of the Korean Wood Science and Technology
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• v.45 no.1
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• pp.85-95
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• 2017

This study aimed at more precisely estimating the age of big old trees using dendrochronological method. Gesan-gun in Chungbuk (CBGS), Gurye-gun in Jeonnam (JNGR) and Uljin-gun in Gyeongbuk (GBUJ) were study areas and Zelkova serrata (ZS) and Pinus densiflora (PD) selected as protected trees therein were used as experimental tree species. The increment cores were extracted from 12, 8, and 6 ZSs and 10, 3, and 9 PDs in CBGS, JNGR, and GBUJ, respectively, using an increment borer (${\phi}5.2mm$). In order to clearly distinguish tree-ring boundary, the surface in the transverse section was cut for ZS using a sliding microtome and sanded for PD using a sand paper. Ring widths were measured in the resolution of 0.01 mm. Based on the measurement values, 203-year long (1813-2015) ZS local master tree-ring chronologies were successfully established and 175-year long (1841-2015) ZS local master tree-ring chronology for JNGR was also successfully established. In the case of PD, 154-, 175-, and 250-year long local master tree-ring chronologies for CBGS, JNGR, and GBUJ were successfully established, respectively. In the comparisons between local master tree-ring chronologies, they showed low t-values and Glks. According to the comparisons of the local master tree-ring chronologies with 50-year (1950~2000) average temperature and precipitation distribution maps, the annual variations of local master tree-ring chronologies seem to be determined by not temperature but precipitation. For such cross-dating therefore more local master tree-ring chronologies have to be established at the least based on the distribution map for precipitation.