• Progress in Superconductivity and Cryogenics
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• v.25 no.3
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• pp.7-12
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• 2023

Isolation of pollutant-degrading bacteria is important in bioaugmentation, one of the methods for biological degradation of environmental contaminants. We focused on the magnetic activated sludge (MAS) process as a culture method that efficiently concentrates degrading bacteria, and cultured activated sludge with 1,4-dioxane as a model pollutant. After 860 days of operation, MLVSS, which indicates the amount of sludge, increased from 390 mg/L to 10,000 mg/L, and the removal rate of organic matter including 1,4-dioxane, tetrahydrofuran, and glucose in the artificial wastewater reached up to 97%. Based on these results, the MAS process was successfully used to acclimate activated sludge with 1,4-dioxane. Bacterial flora analysis in the MAS showed that bacteria of the genus Pseudonocardia, already reported as 1,4-dioxane degrading bacteria, play an important role in the degradation of this pollutant. The MAS process is a suitable culture method for acclimation of environmental pollutants, and the findings indicate that it can be used as an enrichment unit for pollutant-degrading bacteria.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.3
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• pp.417-427
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• 2000

The substrate metabolism and bacterial population in an anaerobic digestion with the submerged separation membrane were investigated by using a laboratory-scale reactor at the hydraulic retention time(HRT) 1.0 and 0.5 day. The removal efficiencies of carbohydrate at the HRT 1.0 and 0.5 day were 99.8~99.9% and 98.0~99.6%, respectively. After the 58 days, the mixed liquor volatile suspended solids(MLVSS) concentration at the HRT 1.0 and 0.5 day were approximately 6,050 and 7,750 mg/L, respectively. According to the measurement by the most probable number(MPN) method, the numbers of acidogenic bacteria, $H_2$-utilizing and acetate-utilizing methc.nogenic bacteria were found to be $10^9$, $10^7{\sim}10^8$ and $10^6{\sim}10^8MPN/mL$, respectively. The composition of $CH_4$ in the produced gas was 46~50%. It is suggested that sulfate-reducing bacteria $10^7{\sim}10^8MPN/mL$ play an important role in producing $H_2$ and acetate in sulfate-depleted environment.

• Journal of Korean Society on Water Environment
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• v.23 no.5
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• pp.776-780
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• 2007

A biofilm reactor packed with porous media was investigated for nitrogen removal of synthetic wastewater. The effect of different loading rates on the nitrification was sustained to be steady state with stable efficiency of 50~60% in the range of $0.0083{\sim}0.017gNH_4-N/gMLVSS{\cdot}day$ of F/MN ratio and $1{\sim}2kgNH_4-N/m^3{\cdot}day$ of media volumetric loading rate. However, nitrification efficiency was rapidly decreased to 25~30% as F/MN ratio and media volumetric loading rate were increased to the range of $0.025{\sim}0.034gNH_4-N/gMLVSS{\cdot}day$ and $3{\sim}4kgNH_4-N/m^3{\cdot}day$, respectively. Also the consumption rate of alkalinity was higher under 8 hours of HRT than unter 6 hours of HRT. Accordingly the influent loading rate variation by detention time with influent flow influenced more on the nitrification efficiency than the influent loading rate variation by the influent concentration did. The temperature effect on the nitrification showed 25% higher in summer than in winter as the results reported by other researchers who reported that the nitrification efficiency in biofilm showed 20% increase from 55% to 75% when the temperature was raised from $20^{\circ}C$ to $25^{\circ}C$. Denitrification with sulfur-media showed 90% removal efficiency under steady-state with no effect from the increase of influent concentration and empty bed contact time (EBCT) change such as EBCT was decreased from 8.4 hr to 4.3 hr and $NO_3-N$ loading rate was changed within the range of $0.1{\sim}0.4kgNO^3-N/m^3{\cdot}day$. Accordingly Denitrification with sulfur-media is feasible for post denitrification at the concentration less than $80mgNO^3-N/L$.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.2
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• pp.113-119
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• 2011

The study purpose was to examine an effect of zero emission of excess sludge on biological reactor and treated water quality within the biological reactor in the process of biological treatment combined with excess sludge reduction system with ozone. Under an ozone injection rate 0.03 g $O_3/g$ SS, Sludge Disintegration Number (SDN) 3 and less than pH 4 as pre-treatment process, it was possible to maintain a stable biological treatment process without sludge disintegration. In the test of $OUR_{max}$, of sludge, its value was hardly under the condition of ozone injection rate 0.03 g $O_3/g$ SS. There were almost no changes of MLVSS/MLSS within biological reactor followed by a solubilization of excess sludge. Accumulation of microorganism within biological reactor was also not observed. After solubilization of excess sludge, an increase for organic matter and SS concentrations of an effluent was not observed and T-N concentration was reduced by increasing nitrification and denitrification rate within biological reactor. Most of T-P was not removed by zero emission of excess sludge and was leaked by being included in effluents.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.2
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• pp.138-144
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• 2005

This study was carried out to investigate the changes of nitrifying bacterial populations including Nitrosomonas sp. and Nitrobacter sp. in $A^2/O$ pilot plant with the configuration of anaerobic-anoxic-oxic reactors. The suspended nitrifying bacterial populations in mixed liquor and those of attached populations on granular carrier surface made by molded waste tire were analyzed by Fluorescent in situ Hybridization(FISH) method. The nitrification rate of a pilot plant showed the value of $1.97{\sim}2.98\;mg\;N/g$ MLVSS hr. The ratios of suspended ammonia oxidizer including Nitrosomonas sp. (NSO) to total bacteria in each reactor were oxic < anoxic < anaerobic. On the contrary, the ratios of suspended nitrite oxidizer including Nitrobacter sp. (NIT) were anaerobic < anoxic < oxic. The thickness, dry density and mass of the attached biomass on granular carriers were $180{\sim}188\;{\mu}m$, $38.5{\sim}43.9\;mg/cm^3$, $29.4{\sim}32.5\;mg/g$, respectively. Also, the ratios of attached nitrifier to total bacteria on granular carriers were similar regardless of ammonia/nitrite-oxidizer (NSO; 3.2%, NIT; 2.8%) and very low compared to those(NSO; $22.8{\sim}28.4%$, NIT; $17{\sim}26%$) of suspended nitrifier.

• Journal of environmental and Sanitary engineering
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• v.5 no.2
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• pp.45-52
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• 1990

This study is to discuss limiting factors influenced on the removal efficiency of organic materials investigated using the polypropyrene biofilter which appropriate to attach micro-organism in order to apply the contact oxidation proce,:5. The results obtained in the experiment were as follows : 1. In the range o: pH 4.0~ 12.0 was obtained the removal efficiency of COD higher than 85% It was proved that variation of pH(4.0 ~ 12.0) was nothing to do with the removal efficiency of substrate in continuous reactor. 2. Temperature to obtain removal efficiency of COD higher than 85% was $10^{\circ}$ ~$40^{\circ}$. Removal efficiency of COD was no less than those at high temperature if MLVSS concentration was maintained 8,000~ 15,000 m/1. 3. In the continuous reactor, the volumetric loading of COD for removal efficiency higher than 95% had to be 0.5~1.5 kg COD/.d below. And then the HRT was Bhrs. 4. In comparison with the conventional activate sludge process, the contact oxidation process was excellent in removal efficiency, sludge production rate and maintenance.

• Journal of Korean Society of Water and Wastewater
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• v.11 no.1
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• pp.64-73
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• 1997

Most of the municipal wastewater treatment plants operated in Korea are designed for high concentrations municipal sewage. However, activated sludge process employed by municipal wastewater treatment plant is operated at low organic loading. The objective of this study was to determine optimum operating condition of activated sludge process for treatment of low concentration municipal sewage. Three bench scale activated sludge reactors were operated to investigate the effect of HRT and SRT on the COD and TSS removal efficiency. The average concentration of TSS, SCOD, SBOD and TKN in influent were 118mg/l, 61mg/l, 21mg/l, and 12mg/l, respectively. The activated sludge reactors operated with various HRT and SRT showed about 89-93% TSS removal efficiency. HRT and SRT does not affect the TSS removal efficiency of actvatied sludge process significantly. However, HRT affected the SCOD removal efficiency slightly. As the HRT decreases from 13hours to 3hours, the SCOD removal efficiency decreases from 67% to 56%. The average effluent TCOD concentration of the reactor operated with 3hours of HRT was approximatly 40-45mg/l. Kinetic coefficient yield (Yt) and decay coefficients(Kd) were 0.594-0.954 mgMLVSS/mgCOD and $0.0197-0.0317day^{-1}$, respectively. Low concentration municipal sewage can be treated with 3 hours of HRT without effluent quality deterioration and SRT does not affect the substrate removal efficiency at this operation condition.

• Journal of Korean Society for Atmospheric Environment
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• v.14 no.6
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• pp.599-606
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• 1998

Removal of toluene vapor from airstreams was studied in a biological reactor known as a biofilter. The biofilter was packed porous ceramic inoculated with thickened activated sludge (MLVSS 17,683 mg/L). The lab-scale biofilter was operated for 42 days under various experimental conditions including inlet toluene concentrations and flow rates of the contaminated air streams. Removal efficiency reached up 96.6% after 4 days from start up. Nutrient limitation was proposed as a reason for the decrease in biofilter performence. Biofilter performance decreased substantially, coincident with the buildup of back pressure due to accumulation of excess VSS within the medium bed. Practically, the bed needs to be backwashed when the overall pressure drop is greater than 460.6 Pa at SV (Space Velocity) 100 h-1. Periodic backwashing of the biofilter was necessary for removing excess biomass and attaining stable long -term high removal efficiency The removal efficiency of toluene in the biofilter decreased as the gas velocity and toluene concentration in the inlet gas increased. The maximum elimination capacity of ceramic biofilter could reach up to 444.85 g/m3. hr. When the loading of toluene exceed this critical value, substrate inhibition occurred.

• Journal of Korean Society of Water and Wastewater
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• v.32 no.1
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• pp.19-25
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• 2018

The objectives of this study were to evaluate the removal characteristics of total nitrogen, the influence factor of denitrification and the optimum operating condition in the pigment wastewater treatment using PAC-A/O process. The operating conditions of PAC-A/O process were mean BOD volumetric loading $0.86kgBOD/m^3/day$, mean F/M ratio 0.072~0.13 kgBOD/kgMLVSS/day and mean C/N ratio 3.47, respectively. The conditions of anoxic process in the field plant test were mean pH 8.3~8.7 and mean temperature $34.1{\sim}44.0^{\circ}C$. The ORP bending point knee was eventually appeared in the ORP -107 mV and $NO_3{^-}-N$ removal efficiency was increased according to the ORP decrease. In the ORP -107 mV below condition, the removal efficiency of T-N and $NO_3{^-}-N$ was 92.3~95.0% and 98.5~99.7%. Denitrification rate was calculated to be 1.581~1.791 mg $NO_3{^-}-N/gMLSS/hr$. The experimental results showed that the ORP control in the PAC-A/O process could be an effective method for treatment of pigment wastewater.

• Journal of Environmental Health Sciences
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• v.43 no.6
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• pp.525-531
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• 2017

Objectives: The purpose of this experiment is to better understand the nitrogen and phosphorus removal ratio according to operating conditions in an iron electrolysis system consisting of an anoxic basin, aerobic basin, and iron precipitation apparatus. Methods: Iron electrolysis consists of an iron precipitation reactor composed of iron plates in oxic and anoxic basins. We studied the interrelation coefficient between T-N and T-P removal rates and F/M ratio, and the C/N ratio and BOD removal rate. Results: The F/M ratio and the T-N and T-P removal rate per unit area have interrelation coefficients of 0.362 and 0.603, respectively. The removal rate per MLVSS and the T-N and T-P removal rate per unit area have respective interrelation coefficients of 0.49 and 0.59. Conclusions: The removal rate of T-N and T-P increased with the increasing F/M ratio in the influent, and they also linearly increased in proportion to the C/N ratio of influent and BOD removal rate of the reactor.