• Journal of the Korean Statistical Society
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• 제27권3호
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• pp.331-348
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• 1998

In this paper, we have proposed some robust Bayes estimators using ML-II priors as well as certain empirical Bayes estimators in estimating the finite population mean in the presence of auxiliary information. These estimators are compared with the classical ratio estimator and a subjective Bayes estimator utilizing the auxiliary information in terms of "posterior robustness" and "procedure robustness" Also, we have addressed the issue of choice of sampling design from a robust Bayesian viewpoint.

• 대한화학회지
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• 제30권5호
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• pp.423-432
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• 1986

폐수 중의 6가크롬을 음이온 교환체인 aliquat-336(tri-caprylmethyl ammonium chloride)으로 이온쌍을 형성시켜 유기용매인 p-xylene으로 추출하여 원자흡수분광도법으로 정량하는 방법에 대하여 조사 검토하였다. Toluene을 용매로 사용하여 유기물을 제거한 폐수 100ml를 취하여 염산으로 pH 0.5 되게 산성화시킨 다음 aliquat-336을 0.01M되게 녹인 p-xylene 20ml로 6가크롬을 추출하였다. 표준물첨가법에 의해 air-acetylene 불꽃에서 용매중에 추출된 6가크롬을 정량하였다. Al(III), Fe(III), Cr(III)이온들은 6가크롬의 추출 및 흡광도 측정에 방해 하지 않았으며, Fe(III)이온이 6가크롬을 3가크롬으로 환원시키기 때문이다. 또한 유기물이 존재하면 6가크롬의 흡광도를 감소시키므로 이런 방해유기물을 toluene으로 추출 제거하였다. 최적 조건으로 분석한 결과는 96%이상의 회수율을 보여 주었고 이는 미량 분석에서 정확한 방법이라 할 수 있고, 상대평균편차가 3.95%로서 재현성도 비교적 좋았다.

• 대한수의학회지
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• 제30권4호
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• pp.515-523
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• 1990

Plasma progesterone concentrations in 44 suckling Korean native cows were determined to monitor ovarian activity postpartum by radioimmunoassay. Blood samples were collected in 3 day intervals from 15 to 80 days postpartum. The ovaries and uterus were examined in 6 day intervals by rectal palpation. Results are summaried as follows: 1. The cows were qualified into four categories; Type I(normal): cyclic changes in plasma progesterone concentrations appear within 45 days postpartum(35 cows, 79.5%), Type II(cycle delayed): delayed resumption of ovarian activity by 55 days postpartum(5 cows, 11. 4%), Type III(cycle ceased with low progesterone): plasma progesterone concentrations remained low(${\leq}1ng/ml$) until 80 days postpartum(3 cows, 6.8%), Type IV(cycle ceased with high progesterone): plasma progesterone concentrations after 30 days remained high(${\geq}4.0ng/ml$) until 80 days postpartum(1 cow, 2.3%). 2. Out of the cows classified Type II and III, 7 cows had inactive ovaries and a cow had follicular cyst by rectal palpation. The cow of Type IV was diagnosed as bearing persistent corpus luteum by rectal palpation. 3. About 82% of the cows showed significant rises in plasma progesterone concentrations prior to 50 days postpartum and the cows of Type II and III resumed cycles in 31. 6(range 17~55) days after calving. However, 43.2% resumed cycles in 50 days postpartum by estrus signs. These results indicated that plasma progesterone concentrations assessed by radioimmunoassay can be utilized for monitoring postpartum ovarian activity and would be helpful for the early detection of ovarian dysfunction in the Korean native cow.

• 한국수정란이식학회지
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• 제23권4호
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• pp.263-267
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• 2008

The purpose of this study was to determine toxic effect of sucrose and trehalose prior to cryopreservation on nuclear maturation and embryonic development in immature bovine oocytes. All cryoprotectant was prepared in tissue culture medium 199-HEPES (TCM 199-HEPES) with 10% fetal bovine serum (FBS). Immature oocytes were exposed to 1.2M ethylene glycol (EG) and 0.1M sucrose or 1.2M EG and 0.1M trehalose for 3 min and then were exposed to 3.2 M EG and 0.25 M sucrose or 3.2 M EG and 0.25 M trehalose for 1 min. Oocytes treated with cryoprotectants were exposed to 0.25 M sucrose or 0.25 M trehalose for 5 min and then 0.1 M sucrose or 0.1 M trehalose for 5 min. Depending on type of sugar added to cryopreservation solution, oocytes were allocated to sucrose group and trehalose group, respectively. Oocytes exposed to TCM 199-HEPES with 10% FBS were considered as control. Oocytes were cultured in TCM 199 supplemented with 10% FBS, 5 ng/ml epidermal growth factor, 0.01 IU/ml luteinizing hormone, and $1\;{\mu}g/ml$ estradiol for 24 h in $39^{\circ}C$, 5% $CO_2$. Nuclear maturation was assessed by staining oocytes with 1% aceto-orcein. Oocytes were fertilized in vitro and were cultured in TCM 199 supplemented with 10% FBS, 5 mM sodium pyruvate, and antibiotics in $39^{\circ}C$, 5% $CO_2$. The rates of cleavage and blastocyst, and cell number in blastocyst were assessed. Metaphase II rates were not different among experimental groups regardless of type of sugar. The cleavage rate of trehalose group (73.3%) was significantly higher (p<0.05) than those of sucrose group (62.8%) and control group (60.8%). The blastocyst rate was significantly higher in trehalose group (p<0.05). Mean cell number in blastocyst were not different among experimental groups, although cell number of blastocyst in trehalose group was significantly higher on day 7 (p<0.05). In conclusion, sucrose and trehalose were not toxic to immature bovine oocytes prior to cryopreservation. In particular, trehalose was more effective on embryonic development.

• 동의생리병리학회지
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• 제22권3호
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• pp.542-547
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• 2008

In order to examine anti-inflammatory effect of Cinnamomi Ramulus (CR) alcohol extract on rheumatoid arthritis, the present study investigated the viability and TNF-${\alpha}$ production in Raw264.7 cells treated with CR and collagen induced arthritis in DBA/1J mice which were orally administered with CR prior to immunization. The results are as follows: CR extract at $20{\mu}g/ml$ inhibited the viability of Raw264.7 by 50%. CR at 15 and $20{\mu}g/ml$ showed a significant decrease in TNF-${\alpha}$ levels from Raw264.7 cells treated with LPS. CR administration decreased arthritic index in DBA/1J mice immunized with bovine collagen type II but it did not reach statistical significance. CR administration significantly decreased spleen weights obtained from mice in 6 weeks after immunization. CR administration significantly decreased serum anti-type II collagen antibody levels compared with control group. CR administration decreased serum IL-6 levels compared with control group but it did not reach statistical significance.

• Restorative Dentistry and Endodontics
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• 제10권1호
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• pp.85-92
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• 1984

The aim of this study was to examine the influence of the speed of grinding and coolants on mercury vaporization during amalgam removal. Forty amalgam filled stone dies were stored at $37^{\circ}C$ and 100% relative humidity for 7 days prior to the beginning of the mercury vapor experiment and were divided into 4 different groups; In Group I; Used by high speed without coolant & evacuator during amalgam removal. In Group II; Used by high speed with coolant & evacuator during amalgam removal. In Group III; Used by low speed without coolant & evacuator during amalgam removal. In Group IV; Used by low speed with coolant & evacuator during amalgam removal. The amalgam specimens were removed in a 30-second time period and mercury vapor was collected with membrane filter at 27mm from the site of removal and 45 degree above there. Samples in Group II, IV were removed with coolant spray at a flow rate of 30 ml/min with high-velocity evacuator. Mercury vapor collected membrane filter was analysed by Atomic Absorption Spectrophotometer using cold vapor method. The results were as follows; 1. The mercury vapor levels were obtained all of the Groups. 2. The mercury vapor levels of the Group II, IV (with coolant & evacuator) were less than that of the Group I, III (without coolant & evacuator). 3. The highest mercury vapor level recorded during amalgam removal procedure was Group I (used by high speed without coolant & evacuator) and its record was $0.78{\pm}0.09\;mg/m^3$, which exceed the T.L.V. by 15 times. 4. The mercury vapor level of the Group IV (used by low speed with coolant & evacuator) was more than that of the Group II (used by high speed with coolant & evacuator), but its difference was not significant, statistically. (p > 0.05)

• Journal of Chest Surgery
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• 제29권8호
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• pp.867-874
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• 1996

이 연구는 대동맥판역류증이 있으면서 좌심실수축말기용적(left ventricular end-systolic volullle)이 커져있는 환자에서 수술전 좌심실 최고수축기압수축말기용적비(left ventricular peak systolic presslle/end-systolic volume ratio)를 판막치환후의 증상적 호전 판단의 자료로서의 적용 여부를 검토하기위한 것이 다 대상은 중정도 이상의 대돈맥판역류증이 있고다른 심혈관계의 이상이 없으면서 좌심실수축말기용적이 60mm2 넘는 21명(남 15, 여 6, 15까에서 60세가지 연령 분포)이었다. 이들을 대상으로 심도자시 통 상적으로 측정하는 여러 변수와 판막치환후 증상적 호전과의 관계에 대해서 분석하였다. 수술 6개월후 증세의 호전이 13명(62%)의 환자에서 있었고, 8명(38%)에서는 증세의 변화가 없었다. 복합변수분석을 통해서 좌심실최고수축기압$\boxUl$수축말기 용적 비는 통계적으로 유의하게 수술 a개월후의 기능적분류상태(p=0.005)와 수술전에 비해 수술 6개월후 기능적분류상태의 변화 정도(p=0.032)를 판단 하는 지표임을 나타냈었다. 판막치환 6개월후 비가 1.71 mmHg/ml/m2 이상되는 모든 환자는 기능상태 I 혹은 II를 유지한 반면 비가 1.71 mmHg ml/m2 미만인 환자에서는 40%가 기능적분류상태 III이 있다. 좌심실 수축말기 용적이 60 ml/m2 이상 커져 있는 대동맥 판역류증 환자에서 좌심실최고수축기 압1수축 말기용적비는 판막치환후의 증상적 호전을 예측하는 지표로 사용될 수 있다.

• 한국발생생물학회지:발생과생식
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• 제4권1호
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• pp.125-131
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• 2000

Differential display-PCR 방법을 이용하여, hCG 처리에 의한 참돔, Pagrus major의 난성숙 능력의 획득 경과시간에 따라 새롭게 발현하는 cDNA를 해석하였다. Differential display-PCR과 5'RACE 방법을 이용하여, 2,662 염기와 434개의 아미노산을 코드하고 있는 cDNA의 전염기배열을 결정하였다. DNA의 데 이터베이스인 GenBank 및 EMBL을 이용하여 상동성을 검색한 결과, 본 cDNA와 높은 상동성을 나타낸 유전자는 검색되지 않았다. 따라서 본 cDNA는 참돔의 난성숙 능력 유도와 함께 그 발현량이 증가하는 난성숙 관련 유전자로 판단되었다. 또한 본 cDNA에서는 protein kinase C 인산화 및 casein kinaseII 인산화 consensus 배열의 존재가 확인되었다. 본 연구에서 cloning된 난성숙 관련 유전자는 난여포에 hCG 처리 9～24시간 후에 그 발현량이 증가하였으며, GH-II (300 ng/ml)로 배양한 난여포에서 특이적으로 증가하였다. 또한 in vivo 실험에서 난성숙 관련 유전자는 난성숙 능력 획득 이전의 난소에서는 거의 발현하지 않았으나, 난성숙 능력을 획득한 난소에서 강하게 발현된 점으로 보아, hCG에 의한 난성숙 능력 유도에 성숙기간 중 새롭게 합성되는 난성숙 관련 유전자가 관여할 가능성이 높다.

• 약학회지
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• 제60권3호
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• pp.112-117
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• 2016

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of caffeine in forensic aqueous sample. The centrifuged sample ($100{\mu}l$) was diluted 50-fold with distilled water. The diluted sample ($400{\mu}l$) was then diluted further with $200{\mu}l$ of 0.1% formic acid solution and $400{\mu}l$ of acetonitrile containing 500 ng of caffeine-(3-methyl-$^{13}C_3$) prior to LC-MS/MS analysis. The mobile phase was composed of 0.1% formic acid in distilled water (A) and acetonitrile (B). Chromatographic separation was performed by using a Zorbax SB-C18 ($100mm{\times}2.1mm$ i.d., $3.5{\mu}m$) column and caffeine was eluted within 1.1 min. Linear least-squares regression with a 1/x weighting factor was used to generate a calibration curve with the coefficients of determination ($r^2=0.9983$). The lower limit of quantification was $25ng/ml$ for the analyte. The process efficiency was 98.6~100.1%. Intra- and inter-day precisions were not more than 2.1% and 1.7%, while intra- and inter-day accuracies were ranged from -6.8 to 4.5%, respectively. The suitability of the method was examined by analyzing unknown forensic aqueous samples.

• Animal cells and systems
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• 제4권3호
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• pp.279-285
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• 2000

Vitamin E-succinate (VES) treatment of U937 human monoblasts induced cells to undergo apoptosis. After 96 h of VES treatment at 10 $\mu$/ml, more than 80% of cells appeared apoptotic. Evidence for apoptosis by VES was based on propidium iodide staining for detection of chromatin condensational fragmentation and electrophoretic DNA ladder formation. Western blot analyses showed a transient increase in Fas and p21 protein levels up to 48 h alter the VES treatment. Protein expression and activity of CDK1 and lamin B degradation were remarkably induced by VES, following the cleavage of caspase-3 after 48 h. The VES-induced apoptosis was found to involve activation of PKC as shown by increases in membrane translocation of PKC$\beat$II and PKC activity. Pretreatment of GF109203X (PKC inhibitor) prior to VES treatment almost completely inhibited the induction of apoptosis as assessed by blockage of VES-induced caspase-3 activity and DNA fragmentation. However, GF109203X h8d no effect on the VES-induced nitric oxide synthesis, which was required for monocvtic differentiation in our previous report (J Cell Sci 111, 435, 1998). Taken together, our data suggest that induction of apoptosis by VES in U937 cells occurs through activation of PKC-$\beat$II resulting in the activation of caspase-3 cascade and is independent of nitric oxide.