• 대한의생명과학회지
• /
• 제19권2호
• /
• pp.153-157
• /
• 2013

Although culture is the gold standard method to identify mycobacteria, its use in tuberculous lymphadenitis (TBL) is limited due to formalin fixation of the submitted specimens. We evaluated the performance of quantitative real-time PCR (q-PCR) for Mycobacterium Tuberculosis (MTB) in granulomatous lymphadenitis using formalin-fixed paraffin-embedded (FFPE) tissues. From 2000 to 2010, a total number of 117 cases of lymph node samples with granulomatous inflammation which were surgically removed and fixed in formalin were studied. Hematoxylin & Eosin (H&E) and Ziehl-Neelsen-stained (ZN) slides were reviewed. qPCR using Real TB-Taq$^{(R)}$ was performed for all cases to identify Mycobacterium tuberculosis. Thirteen non-tuberculous lymphadenopathy cases were used as negative control. Cervical lymph nodes were more frequently affected (60%, 70/117) than other sites. ZN stain for acid fast bacilli was positive in 19 (16.24%) cases. qPCR for tuberculosis was positive in 92 (78.63%) cases. Caseous necrosis was found in 103 (88.03%) cases. While the ZN stain and qPCR were both negative in all control cases, the qPCR showed a significantly higher positive rate (78.63% vs. 16.24%) compared to ZN stain in histologically diagnosed TBL. Quantitative real-time PCR proves to be more sensitive than ZN stain for diagnosis of tuberculous lymphadenitis.

• 한국ITS학회 논문지
• /
• 제3권2호
• /
• pp.75-83
• /
• 2004

2004. 7. 1. 서울시는 대중교통 체계 개편의 하나로 강남대로, 성산-수색로, 도봉-미아로에 중앙버스전용차로제를 확대 시행하고 있다. 1996년부터 천호대로에서 실시한 중앙버스전용차로의 속도가 평균 35km/h를 나타내고 있는 것을 보면 버스의 속도 향상은 분명한 것으로 판단된다. 중앙버스전용차로를 시행하면 교차로상의 좌회전이 문제가 된다. 버스가 기존 1차로를 진행하기에 좌회전 차량들은 2차로에서 좌회전 할 수밖에 없다 원래 중앙버스전용차로를 실시하면 교차로의 좌회전은 허용치 않아야 한다. 하지만 좌회전을 허용치 않을 경우 지역 주민들의 반발과 다른 우회로 확보의 어려움 등의 문제가 제기된다. 이에 본 연구에서는 중앙버스전용차로를 시행하면서 기존의 교차로 좌회전은 허용한다는 전제하에 교차로 좌회전을 보다 안전하게 할 수 있는 방안으로 교차로 정지선을 분리하고, 그 적정 거리 산출 방안을 제시하고자 한다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권5호
• /
• pp.1925-1928
• /
• 2015

Background: To investigate whether CT findings can predict the invasiveness of persistent cancerous pure ground glass opacity (pGGO) by correlating the CT imaging features of persistent pGGO with pathological changes. Materials and Methods: Ninety five patients with persistent pGGOs were included. Three radiologists evaluated the morphologic features of these pGGOs at high resolution CT (HRCT). Binary logistic regression was used to assess the association between CT findings and histopathological classification (pre-invasive and invasive groups). Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic performance of diameters. Results: A total of 105 pGGOs were identified. Between pre-invasive (atypical adenomatous hyperplasia, AAH, and adenocarcinoma in situ, AIS) and invasive group (minimally invasive adenocarcinoma, MIA and invasive lung adenocarcinomas, ILA), there were significant differences in diameter, spiculation and vessel dilatation (p<0.05). No difference was found in air-bronchogram, bubble-lucency, lobulated-margin, pleural indentation or vascular convergence (p>0.05). The optimal threshold value of the diameters to predict the invasiveness of pGGO was 12.50mm. Conclusions: HRCT features can predict the invasiveness of persistent pGGO. The pGGO with a diameter more than 12.50mm, presences of spiculation and vessel dilatation are important factors to differentiate invasive adenocarcinoma from pre-invasive cancerous lesions.

• 대한치과교정학회지
• /
• 제39권4호
• /
• pp.257-272
• /
• 2009

본 증례보고는 I급 견치 및 구치 관계를 보이나, 비강기도 협소에 따른 하안면 고경의 과도한 증가와 양 악궁의 폭경 감소 뿐만 아니라, 하악골의 후방위치 및 현저한 입술의 돌출로 인해 돌출된 안모를 지닌 성인 환자의 치료를 소개하고자 한다. 협착된 양 악궁은 수술을 동반한 급속 구개확장과 슈발츠 장치로써 확장되었으며, 마이크로임플란트를 이용한 directional force technology를 적용하여 상하악 구치부에서의 고정원 보강과 상악 전치부의 토크 조절 및 시계 반대 방향으로의 양호한 mandibular response를 얻음으로써 양호한 안모의 균형을 얻을 수가 있었다. 총 치료기간은 29개월 소요되었고, 장치 제거 후 13개월 유지 후에도 양호한 결과가 유지되었다.

• 보건행정학회지
• /
• 제25권4호
• /
• pp.277-284
• /
• 2015

Background: This study investigates the relationship of socioeconomic status with adverse birth outcomes (low birth weight, preterm birth) and the relationship of socioeconomic status with infant mortality, using the birth cohort in Korea, 1995-2010. Methods: 8,648,035 births from National Statistics Offics, 1995-2010 were studied with respect to social variation in adverse birth outcomes and infant mortality in Korea. The effect of social inequality was examined against adverse birth outcomes and infant mortality using multivariate logistic regression after controlling for other covariates. Results: Social inequality were observed in adverse birth outcomes: low birth weight (LBW, 1,500-2,499 g), very LBW (1,000-1,499 g), and extremely LBW (500-999 g) as well as moderately preterm birth (PTB, 33-36 weeks), very PTB (28-32 weeks), extremely PTB (22-27 weeks), and infant mortality. The effect of social inequality was higher among moderately LBW (1,500-2,499 g) and PTB (33-36 weeks) than very or extremely LBW and PTB. Conclusion: The social inequality in adverse birth outcomes (low birth weight and preterm) and infant mortality existed and increased in Korea from 1995 to 2010. The effect of maternal education on adverse birth outcomes as well as infant mortality was apparent in the study results. Especially, social inequailiy in infant mortality was greater among the sub-normal births (low birth weight [1,500-2,499 g] or preterm birth [33-36 weeks]), which suggests, social interventions should aim at more among the subnormal births. This study suggest that tackling inequality in births as well as infant mortality should be focused on the social inequality itself.

• The Plant Pathology Journal
• /
• 제15권1호
• /
• pp.21-27
• /
• 1999

Nonpathogenic mutants of Xanthomonas campestris pv. glycines were generated with Omegon-Kim to isolate genes essential for pathogenicity and inducing hypersensitive response (HR). Three nonpathogenic multants and two mutants showing slow symptom development were isolated among 1,000 colonies tested. From two nonpathogenic mutants, 8-13 and 26-13, genes homologous to hrcC and hrpF of X. campestris pv. vesicatoria were identified. The nonpathogenic mutant 8-13 had a mutation in a gene homologous to hrpF of X. campestris pv. vesicatoria and failed to cause HR on pepper plants but still induced HR on tomato leaves. The nonpathogenic mutant 26-13 had an insertional mutation in a gene homologous to hrcC of X. campestris pv. vesicatoria and lost the ability to induce HR on pepper leaves but still caused HR on tomato plants. Unlike other phytopathogenic bacteria, the parent strain and these two mutants of X. campestris pv. glycines did not cause HR on tobacco plants. a cosmid clone, pBL1, that complemented the phenotypes of 8-13 was isolated. From the analysis of restriction enzyme mapping and deletion analyses of pBL1, a 9.0-kb Eco RI fragment restored the phenotypes of 8-13. pBL1 failed to complement the phenotypes of 26-13, indicating that the hrcC gene resides outside of the insert DNA of pBL1. One nonpathogenic mutant, 13-33, had a mutation in a gene homologous to a miaA gene encoding tRNA delta (2)-isopentenylpyrophosphate transferase of Escherichia coli. This indicated that tRNA modifications in X. campestris pv. glycines may be required for expression of genes necessary for pathogenicity. The mutant 13-33 multiplied as well as the parent strain did in the culture medium and in planta, indicating that loss of pathogenicity is not due to the inability of multiplication in vivo.

• 대한물리의학회지
• /
• 제8권4호
• /
• pp.539-547
• /
• 2013

PURPOSE: This study examined the effects of exercise of diverse intensities on the expression of TNF-${\alpha}$(tumor necrosis factor-${\alpha}$) in the spinal cord in osteoarthritic rats. METHODS: Over a period of four weeks, the authors applied treadmill exercise programs of diverse intensities to Sprague-Dawley rats, to which intra-articular injection of monosodium iodoacetate(MIA, $3mg/50{\mu}l$, diluted in saline) was applied to the right knee joint to induce osteoarthritis. The four-week exercise program was not carried out with the control group(CG, n=10). Exercise programs of applicable intensities were applied to the low-intensity exercise group(LEG, n=10), moderate-intensity exercise group (MEG, n=10), and high-intensity exercise group(HEG, n=10) over the four weeks. Observations were made of expression of TNF-${\alpha}$ in the spinal cord in osteoarthritic rats using western blot analysis. RESULT: there were significant differences(p<.05) in the comparison of expression of TNF-${\alpha}$ between the four groups involved. The expression of TNF-${\alpha}$ in the spinal cord, the LEG and HEG had more elevated expression significantly than the CG(p<.05). But the MEG had reduced expression significantly than the CG(p<.05). CONCLUSION: These study results suggest that moderate-intensity exercise is effective in inhibition TNF-${\alpha}$ in the spinal cord. They also indicate that in prescribing exercise to treat osteoarthritic patients, exercise of moderate intensity is most suitable to patients' chronic pain, rather than low or high intensity.

• 대한교통학회지
• /
• 제28권6호
• /
• pp.85-97
• /
• 2010

본 연구는 정류소에서 여러노선의 버스가 동시에 몰려들어오는 버스몰림운행의 실태를 파악하고 이로 인해서 야기되는 문제점을 개선하기 위하여 버스몰림 운행지속시간 모형을 추정하고 버스몰림운행에 영향을 미치는 요인을 분석하였다. 버스몰림지속시간의 관측치를 대상으로 8개의 지속시간에 대한 분포를 검토하여 가장 적합도가 높게 나타난 weibull 분포를 가정한 모형을 개발하였다. 18개 영향변수를 검토하였으며 정차면수, 정차면수당 노선수, 노선당 최대 승하차인원을 설명변수로 하는 버스몰림운행 지속시간 모형을 개발하였다. 중앙버스전용차로(도봉미아로)와 가로변 전용 및 일반차로(통일의주로)에 대한 모형을 각각 도출하여 비교하였다. 그 결과, 도봉미아로의 몰림지속시간은 통일의주로에 비해 교통량에 대한 영향을 많이 받으며, 통일의주로는 도봉미아로에 비해 정차면수와 정차면수당 노선수, 노선당 최대 승하차인원에 많은 영향을 받는 것으로 나타났다. 개발된 모형을 토대로 버스몰림운행 현상의 완화방안을 제시하였다.

• 한국콘텐츠학회논문지
• /
• 제14권2호
• /
• pp.343-354
• /
• 2014

본 연구는 중년기 성인을 대상으로 부부갈등이 부부 적응에 미치는 영향을 파악하고, 부부갈등과 부부적응의 관계에서 긍정감정 및 성역할태도의 매개효과를 검증하기 위해 구조방정식 모델을 적용하여 분석하였다. 연구 참여자는 총 270명의 중년기 기혼 남녀이며 구조화된 설문지를 이용하여 자료를 수집하였으며 2012년 4월 12일에서 6월 24일까지 자료를 수집하였다. 연구결과, 부부갈등은 부부 적응에 유의한 직접적인 영향력을 가졌고 긍정감정은 부부갈등과 부부 적응 간의 관계에서 유의한 부분매개효과를 보였으나 성역할태도는 부부갈등과 부부 적응간의 관계에서 매개관계가 성립되지 않았다. 수정모형의 적합도 지수들은 양호하게 나타났으며( ${\chi}^2$=38.90 표준${\chi}^2$=1.77(CMIN/df), GFI=.97, AGFI=.94, NFI=.98, SRMR=.02, CFI=.99, RMSEA=.05) 부부 적응에 대한 모형의 설명력은 88.9%였다. 이러한 결과는 부부갈등과 긍정감정을 변화시킴으로서 중년기의 부부 적응을 증진시킬 수 있음을 의미한다. 본 연구결과는 효율적인 부부교육 프로그램 개발 및 부부관계증진 전략 개발에 기여할 것이다.

• 무역상무연구
• /
• 제43권
• /
• pp.239-273
• /
• 2009

Marine insurance contracts, which intended to provide indemnity against marine risks upon the payment of a premium, originated in Northern Italy in the late 12th and early 13th centuries. The law and practice of Italian merchants were later introduced into England through Lombard merchants. It is, therefore, quite exact that English and Continental marine insurance law have common root. Nevertheless, some significant divergences between English and Continental marine insurance systems occurred since the late 17th century, mainly due to different approaches adopted by English courts. The rule of warranty in English marine insurance was established in the second part of the 18th century by Lord Mansfield, who laid the foundations of the modern English law of marine insurance and developed different approaches, especially in the field of warranty in marine insurance law. Since the age of Lord Mansfield, English marine insurance law has developed a unique rule on warranty. Bearing in mind the realities of the 18th century, it could easily be understood why Lord Mansfield afforded such a strict legal character to marine warranties. At that time, the 'promise' given by the assured, played an important role for the insurer to assess the scope of the risk. Legal environments, however, have changed dramatically since the times of Lord Mansfield. Of course, it is still important that the assured keep his promises to the insurer under the insurance contract, which is based upon utmost good faith. Nevertheless, the remedy of automatic discharge from liability, regardless of existence of a casual link between the breach and loss seems harsh in the realities of the 21st century. After examining the warranty regime adopted by the German and Norwegian hull clauses, it is fair to say that they provide a more equitable approaches for the assured than does English law. Therefore, this article suggests that English warranty regime needs overall reform and it is time to reform.