• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.155-161
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• 2009

Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.

• 자원환경지질
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• 제39권3호
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• pp.255-268
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• 2006

58개 기상관측소에서 최근 22년간(1981-2002) 측정된 기상 자료를 이용하여 국내의 기온(SAT) 및 지면온도(GST)의 시공간적 변동 경향을 분석하였다. 먼저 관측 자료로부터 각 관측소의 평균기온(MSAT)과 평균지면온도(MGST)를 계산하였으며, 다중선형회귀분석을 통해 MSAT와 MGST를 예측할 수 있는 회귀식을 산정하였다. 회귀모형의 회귀변수는 관측소의 위도 및 고도이다. 회귀모형의 추정치와 실제 관측값의 결정계수($R^2$)는 각각 0,92와 0.94로 나타나 모형의 예측 정확성이 매우 높은 것으로 분석되었다. MGST는 지열펌프 시스템 설계의 주요 입력 변수이므로 최근 지열에너지자원 활용 분야에서 매우 중요하게 다루어지는 변수이다. 따라서 제시된 회귀모형은 신뢰할만한 관측 자료가 없는 지역에서 MGST를 추정하는데 매우 유용하게 이용될 수 있을 것으로 예상된다. SAT 자료에 대한 선헝회귀분석을 통해 지구온난화 및 도시화에 기인한 기온 상승의 장기 추세 변동성을 탐색하였다. 1개 관측소를 제외한 57개 관측소에서 $0.005{\sim}0.088^{\circ}C/yr$ 범위의 기온증가율을 가지는 추세 변동이 확인되었다. 또한 GST에 영향을 미치는 기상요소로서 일사량, 지구복사, 강수량 및 적설량 자료를 분석하였다. GST는 주로 SAT 및 일사량에 의하여 결정되지만 강수 및 증발에 의한 토양의 열용량 변화, 적설에 의한 대기와 지표면 차단, 지구복사에 영향을 줄 수 있는 대기의 조건 변화 등이 복합적인 변동 요인으로 작용하는 것으로 나타났다.

• 한국멀티미디어학회논문지
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• 제11권6호
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• pp.764-776
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• 2008

Image identification is the process of checking whether the query image is the transformed version of the specific original image or not. In this paper, image identification method based on feature composition is proposed. Used features include color distance, texture information and average pixel intensity. We extract color characteristics using color distance and texture information by Modified Generalized Symmetry Transform as well as average intensity of each pixel as features. Individual feature is quantized adaptively to be used as bins of histogram. The histogram is normalized according to data type and it is used as the signature in comparing the query image with database images. In matching part, Manhattan distance is used for measuring distance between two signatures. To evaluate the performance of the proposed method, independent test and accuracy test are achieved. In independent test, 60,433 images are used to evaluate the ability of discrimination between different images. And 4,002 original images and its 29 transformed versions are used in accuracy test, which evaluate the ability that the proposed algorithm can find the original image correctly when some transforms was applied in original image. Experiment results show that the proposed identification method has good performance in accuracy test. And the proposed method is very useful in real environment because of its high accuracy and fast matching capacity.

• Nutritional Sciences
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• 제7권1호
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• pp.8-16
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• 2004

The purpose of this study was to investigate the gene profiles that were up- or down-regulated in the livers of high-fat diet-induced obese mice and $db_-/db_-$ mice with deficient leptin receptor. C57/BL6 normal mice and $db_-/db_-$ mice, respectively, were divided into two groups and fed a standard or high-fat diet for four weeks. Liver weight was unchanged in the normal mice but the high-fat diet led to a 10% weight increase in the $db_-/db_-$mice. Adipose tissue mass increased by about 88% in the normal mice that were fed a high-fat diet and by about 17% in the $db_-/db_-$mice on the high-fat diet. In terms of serum lipids, total cholesterol significantly increased in mice on the high-fat diet. Microarray analysis was carried out using total RNA isolated from the livers of standard or high-fat diet-fed mice of the normal and $db_-/db_-$ strains. The change of gene expression was confirmed by RT-PCR. About 1.6% and 6.8% of total genes, respectively, showed different expression patterns in the normal mice fed the high-fat diet and $db_-/db_-$ mice. As a result of microarray, many genes involved in metabolism and signal pathways were shown to have different expression patterns. Expression of Mgst3 gene increased in the livers of normal and $db_-/db_-$ mice that were fed a high-fat diet. Wnt7b and Ptk9l were down-regulated in the livers of the normal mice and $db_-/db_-$ mice that were fed a high-fat diet. In conclusion, a high-fat diet induced obesity and affected gene expression involved in metabolism and signal pathway.