• Title/Summary/Keyword: ME test

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Evaluation of Amino Acid and Energy Utilization in Feedstuff for Swine and Poultry Diets

  • Kong, C.;Adeola, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.7
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    • pp.917-925
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    • 2014
  • An accurate feed formulation is essential for optimizing feed efficiency and minimizing feed cost for swine and poultry production. Because energy and amino acid (AA) account for the major cost of swine and poultry diets, a precise determination of the availability of energy and AA in feedstuffs is essential for accurate diet formulations. Therefore, the methodology for determining the availability of energy and AA should be carefully selected. The total collection and index methods are 2 major procedures for estimating the availability of energy and AA in feedstuffs for swine and poultry diets. The total collection method is based on the laborious production of quantitative records of feed intake and output, whereas the index method can avoid the laborious work, but greatly relies on accurate chemical analysis of index compound. The direct method, in which the test feedstuff in a diet is the sole source of the component of interest, is widely used to determine the digestibility of nutritional components in feedstuffs. In some cases, however, it may be necessary to formulate a basal diet and a test diet in which a portion of the basal diet is replaced by the feed ingredient to be tested because of poor palatability and low level of the interested component in the test ingredients. For the digestibility of AA, due to the confounding effect on AA composition of protein in feces by microorganisms in the hind gut, ileal digestibility rather than fecal digestibility has been preferred as the reliable method for estimating AA digestibility. Depending on the contribution of ileal endogenous AA losses in the ileal digestibility calculation, ileal digestibility estimates can be expressed as apparent, standardized, and true ileal digestibility, and are usually determined using the ileal cannulation method for pigs and the slaughter method for poultry. Among these digestibility estimates, the standardized ileal AA digestibility that corrects apparent ileal digestibility for basal endogenous AA losses, provides appropriate information for the formulation of swine and poultry diets. The total quantity of energy in feedstuffs can be partitioned into different components including gross energy (GE), digestible energy (DE), metabolizable energy (ME), and net energy based on the consideration of sequential energy losses during digestion and metabolism from GE in feeds. For swine, the total collection method is suggested for determining DE and ME in feedstuffs whereas for poultry the classical ME assay and the precision-fed method are applicable. Further investigation for the utilization of ME may be conducted by measuring either heat production or energy retention using indirect calorimetry or comparative slaughter method, respectively. This review provides information on the methodology used to determine accurate estimates of AA and energy availability for formulating swine and poultry diets.

Enhanced Carbon Dioxide Adsorption on Post-Synthetically Modified Metal-Organic Frameworks

  • Ko, Na-Keun;Kim, Ja-Heon
    • Bulletin of the Korean Chemical Society
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    • v.32 no.8
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    • pp.2705-2710
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    • 2011
  • Four MOFs functionalized with 1-Me, 1-Pr, 1-Ph, and 1-$PhCF_3$ were prepared through post-synthetic modifications of a metal-organic framework (MOF), UMCM-1-$NH_2$ (1) with acetic, butyric, benzoic, and 4-(trifluoromethyl)benzoic anhydrides, respectively. Methane adsorption measurements between 253 and 298 K at pressures up to 1 bar indicated that both 1-Ph and 1-$PhCF_3$ adsorbed more $CH_4$ than the parent MOF, 1. All the functionalized MOFs adsorbed more $CO_2$ than 1 under conditions similar to the $CH_4$ test. The introduction of functional groups promoted adsorption of both $CH_4$ and $CO_2$ despite significantly reducing Brunauer-Emmet-Teller (BET) surface area: 4170 (1), 3550 (1-Me), 2900 (1-Pr), 3680 (1-Ph), and 3520 $m^2/g$ (1-$PhCF_3$). Electron-withdrawing aromatic groups (1-Ph, 1-$PhCF_3$) more effectively enhanced $CO_2$ adsorption than electron-donating alkyl groups (1-Me, 1-Pr). In particular, 1-Ph adsorbed 23% more $CO_2$ at 298 K and 50% more at 253 K than 1.

Acute Oral Toxicity Study on BDR-29 in Rats (랫트에서 한약재 복합물 BDR-29의 단회 경구투여 독성에 관한 연구)

  • Kim, Eun-Soon;Chang, Bo-Yoon;Lee, An-Sook;Kang, Dae-Gill;Lee, Ho-Sub;Kim, Byung-Doo;Kim, Sung-Yeon
    • Korean Journal of Pharmacognosy
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    • v.38 no.1
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    • pp.71-75
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    • 2007
  • Acute toxicity of BDR-29, a combined preparation of Cassia Semen, Prunellae Spica, Tribuli Fructus, and Uncariae Rhamulus et Uncus was examined using male and female Sprague-Dawley rats. Rats were treated with the BDR-29 intra-gastrically at 0 mg/kg, 5 mg/kg, 50 mg/kg, 500 mg/kg or 2,000 mg/kg and observed for two weeks. At the doses used no mortality or abnormal clinical signs in animals were shown during at the observation period. Also there was no difference in net body weight gain, gross pathological findings, and urine analysis at the terminal sacrifice among the groups rats treated with different doses of the test substance. The results suggested that acute oral toxicity of BDR-29 in rats is very low at the conditions employed in this study.

Study on Performance and Meat Characteristics in Korean Native Commercial Chicken II. Study on Meat Characteristics in Korean Native Commercial Chicken by Feeding System (한국재래닭의 육용실용계의 발육 및 육질특성 구명 연구 2. 사료 급여체계에 따른 한국재래닭 육용실용계 계육의 특성)

  • 강보석;이상진;김상호;서옥석;나재천;장병귀;박범영;이종문;오봉국
    • Korean Journal of Poultry Science
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    • v.25 no.3
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    • pp.137-145
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    • 1998
  • This study was conducted to compare the meat characteristics Korean Native Commercial Chicken(KNCC) by feeding system Feeding system of T$_1$ and T$_2$were same types from hatch to 8 weeks, starter diets( 0~4 weeks, mash, ME 3,l00kcal, CP 22.94%), grower diets(4~8 weeks, crumble, ME 3,l00kcal, CP 19.31%). Nutrient contents of finisher diets of T$_1$(pellet, M.E 3, 200kcal, CP 20.44%) was higher than T$_2$(mash, ME 3,l00kcal, CP 14.88%). Breast and legs meat of KNCC were used to analyze chemical composition, physicochemical characteristics, textural traits and sensory evaluation test. Live weight(L.W) /abdominal fat accumulation(A.F.A) was significantly different(P<0.05), but live weight(L.W) /dressed weight(D.W), percentage of cutted meat, chemical composition(moisture, CF, EE, CA), meat color(CIE), physicochemical properties, sensory evaluation score were not significantly by feeding systerns, at 12 ar'd 16 weeks of age.

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Evaluation of Mulberry Germplasm (Morns spp.) for Leaf Yield and Quality through Bioassay

  • Tikader, A.;Kamble, C. K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.14 no.2
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    • pp.87-92
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    • 2007
  • Twenty - four elite mulberry germplasm each of indigenous and exotics were studied for their leaf yield and compared with commercial check ($V_1$ and Kosen). Accession MI-0416 and ME-0169 out yielded the check accession in leaf yield/plant. The other few mulberry germplasm were also performed at par with the checks. For quality test and bioassay were conducted with the leaves of selected mulberry germplasm. Among the selected twelve mulberry accessions used for bioassay, MI-0376 and ME-033 performed better than check ($V_1$, Kosen). Other mulberry accessions i.e., MI-0310 and MI-0437 are on par with the check as far as the bioassay is concerned. MI-0376 and ME-0033 out yielded in rearing parameters and qualified for 11 and 10 rearing and related traits. Other mulberry accessions i.e., MI-0310 and MI-0437 were also qualified for eight rearing traits along with check ($V_1$). The mulberry accessions tested after selection from the preliminary characterization seems to be better and equally good in rearing and leaf yield compared to check ($V_1$, Kosen), which provides scope for selection and further evaluation. The selected mulberry accession may be included in crop improvement programme.

Development of a Framework to Estimate the EEOI of a Ship Considering the Hydrodynamic Characteristics and Engine Mode (선박의 유체동역학 특성 및 엔진 모드를 고려한 에너지효율운항지수 추정 프레임워크 개발)

  • You, Youngjun;Park, Hongrae
    • Journal of the Society of Naval Architects of Korea
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    • v.55 no.6
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    • pp.457-465
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    • 2018
  • Since IMO has discussed the effectuation of the EEDI, EEOI and SEEMP, each country, shipping company, shipbuilding company and research institute have been requested to prepare the design, construction and operation of the efficient ship. From the shipbuilding company's point of view, it was necessary to develop a method based on the maneuvering equations of motion in a bid to estimate the EEOI considering the design, model test results and the calculation results of the ship. In this paper, the estimation method of RPM, power and fuel consumption proposed in the previous research was developed to construct a framework that helps in the estimation of the EEOI. It was possible to estimate the EEOI from the estimated ship speed (distance), LNG cargo mass, fuel consumptions and emission factors according to the type of fuel. The rapid increase of the evaluated EEOI was observed when the LNGC with ME-GI engine executing the course changed with a large difference. This prompted the comparison of the type of fuel on the estimated EEOI by considering HFO, LNG fuel and MGO properties.

Maximum A Posteriori Estimation-based Adaptive Search Range Decision for Accelerating HEVC Motion Estimation on GPU

  • Oh, Seoung-Jun;Lee, Dongkyu
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.13 no.9
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    • pp.4587-4605
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    • 2019
  • High Efficiency Video Coding (HEVC) suffers from high computational complexity due to its quad-tree structure in motion estimation (ME). This paper exposes an adaptive search range decision algorithm for accelerating HEVC integer-pel ME on GPU which estimates the optimal search range (SR) using a MAP (Maximum A Posteriori) estimator. There are three main contributions; First, we define the motion feature as the standard deviation of motion vector difference values in a CTU. Second, a MAP estimator is proposed, which theoretically estimates the motion feature of the current CTU using the motion feature of a temporally adjacent CTU and its SR without any data dependency. Thus, the SR for the current CTU is parallelly determined. Finally, the values of the prior distribution and the likelihood for each discretized motion feature are computed in advance and stored at a look-up table to further save the computational complexity. Experimental results show in conventional HEVC test sequences that the proposed algorithm can achieves high average time reductions without any subjective quality loss as well as with little BD-bitrate increase.

Glycosylation of Semi-Synthetic Isoflavene Phenoxodiol with a Recombinant Glycosyltransferase from Micromonospora echinospora ATCC 27932

  • Seo, Minsuk;Seol, Yurin;Park, Je Won
    • Journal of Microbiology and Biotechnology
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    • v.32 no.5
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    • pp.657-662
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    • 2022
  • Glycosyltransferase (GT)-specific degenerate PCR screening followed by in silico sequence analyses of the target clone was used to isolate a member of family1 GT-encoding genes from the established fosmid libraries of soil actinomycetes Micromonospora echinospora ATCC 27932. A recombinant MeUGT1 was heterologously expressed as a His-tagged protein in E. coli, and its enzymatic reaction with semi-synthetic phenoxodiol isoflavene (as a glycosyl acceptor) and uridine diphosphate-glucose (as a glycosyl donor) created two different glycol-attached products, thus revealing that MeUGT1 functions as an isoflavonoid glycosyltransferase with regional flexibility. Chromatographic separation of product glycosides followed by the instrumental analyses, clearly confirmed these previously unprecedented glycosides as phenoxodiol-4'-α-O-glucoside and phenoxodiol-7-α-O-glucoside, respectively. The antioxidant activities of the above glycosides are almost the same as that of parental phenoxodiol, whereas their anti-proliferative activities are all superior to that of cisplatin (the most common platinum chemotherapy drug) against two human carcinoma cells, ovarian SKOV-3 and prostate DU-145. In addition, they are more water-soluble than their parental aglycone, as well as remaining intractable to the simulated in vitro digestion test, hence demonstrating the pharmacological potential for the enhanced bio-accessibility of phenoxodiol glycosides. This is the first report on the microbial enzymatic biosynthesis of phenoxodiol glucosides.

Ameliorating Effect of the Root Extract from Platycodon grandiflorum on the Ethanol-induced Cognitive Impairment in Mice (에탄올 투여로 유도한 생쥐의 인지기능 손상에 대한 길경 추출물의 개선효능)

  • Kim, Young-Sup;Kim, Jung-Sook;Choi, Yeon-Hee;Seo, Jee-Hee;Lee, Jung-Won;Kim, Seong-Kie;Kang, Jong-Seong;Lee, Hyun-Sun;Cho, Young-Soo;Roh, Seong-Hwan;Jeong, Young-Chul;Shim, Ki-Hwan;Ryu, Shi-Yong
    • Korean Journal of Pharmacognosy
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    • v.35 no.3 s.138
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    • pp.239-243
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    • 2004
  • The ameliorating effect of the MeOH extract of the root of Platycodon grandiflorum on the ethanol-induced cognitive dysfunction in mice was investigated. The total MeOH extract was devided into two fractions, saponin rich fraction and non-saponin fraction. The mice with repeated administration of the total MeOH extract and those with saponin rich fraction both showed a markedly prolongεd latency time upon the step-through test (passive avoidance performance) performed after acute ethanol intoxication, while those with non-saponin rich fraction did not. The results suggest that the extracts from the root of Platycodon grandiflorum exert a beneficial effect on ethanol-induced memory impairment in mice and also suggest that these effects of the extracts might be ascribed to the saponin constituents.

Active Compounds from The Aerial Parts of Polygonum multiflorum Thunberg (하수오 지상부의 활성 성분)

  • Kim, Jin-Pyo;Kim, Do-Hoon;Kim, Gi-Eun;Kim, Jin-Hyo;Chung, Sung-Hee;Kim, Sung-Gun;Whang, Wan-Kyunn
    • YAKHAK HOEJI
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    • v.50 no.6
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    • pp.351-357
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    • 2006
  • Polygoni Radix (Polygonaceae) has been used as renal disoder hematopoietic, menstrual irregularity and antiaging in Korean folk medicine. Specially aerial parts has been used for insomnia and sedative agent. In order to investigate the efficacy of antioxidant activity on these aerial parts, the bio-activity guided fraction and isolation of physiologically active substance were performed. H$_2$O, 30%, 60%, 100% MeOH were examined antioxidant activity by DPPH method. It was revealed that 30%, 60% MeOH fractions have significantly antioxidant activity. From 60% MeOH fraction, six known flavonoid glycosides (quercetin, quercetin 3-O-${\beta}$-D-galactopyranosyl-4'-O-glucopyrano side-3'-methyl ether, quercetin 3-O-${\alpha}$-L-rhannopyranoside, quercetin 3-O- ${\alpha}$-L-rhamnopyranoside-4'-methylethor, quorcetin 3-O-${\beta}$-D-galactopyranoside, quercetin 3-O-${\beta}$-D-galactopyranoside) and 3,4-Oihydrocinnarnic acid were isolated. To investigate the antioxidant activities of each com-pounds, we measured radical scavening activity with DPPH method and anti-lipid peroxidative efficacy on low density lipo-protein (LDL) with TBARS assay and anti-acetylcholinestrase activity with Ellman's Test. Four compounds (ll, IV Vl, Vll) of quercetin glycosides showed significant activity.