• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.955-963
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• 2023

Chlorella is a eukaryotic organism that can be used as an industrial host to produce recombinant proteins. In this study, a salt-inducible promoter (SIP) was isolated from the freshwater species Chlorella vulgaris PKVL7422 from the screening of genes that were upregulated after salt treatment. Several cis-acting elements, including stress response elements, were identified in the isolated SIP. Moreover, the Gaussia luciferase gene was cloned after the SIP and transformed into C. vulgaris to test the inducibility of this promoter. Reexamination of transcriptome of C. vulgaris revealed that genes involved in the synthesis of methyl jasmonic acid (MeJA), gibberellin (GA), and abscisic acid (ABA) were upregulated when C. vulgaris was treated with salt. Furthermore, the expression level of recombinant luciferase increased when the transformed C. vulgaris was treated with salt and MeJA, GA, and ABA. This study represents the first report of the C. vulgaris SIP and highlights how transformed microalgae could be used for robust expression of recombinant proteins.

• Journal of Yeungnam Medical Science
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• 제40권4호
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• pp.321-327
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• 2023

Recently, the International Working Group on the Diabetic Foot and the Infectious Diseases Society of America divided diabetic foot disease into diabetic foot infection (DFI) and diabetic foot osteomyelitis (DFO). DFI is usually diagnosed clinically, while numerous methods exist to diagnose DFO. In this narrative review, the authors aim to summarize the updated data on the diagnosis of DFO. An extensive literature search using "diabetic foot [MeSH]" and "osteomyelitis [MeSH]" or "diagnosis" was performed using PubMed and Google Scholar in July 2023. The possibility of DFO is based on inflammatory clinical signs, including the probe-to-bone (PTB) test. Elevated inflammatory biochemical markers, especially erythrocyte sedimentation rate, are beneficial. Distinguishing abnormal findings of plain radiographs is also a first-line approach. Moreover, sophisticated modalities, including magnetic resonance imaging and nuclear medicine imaging, are helpful if doubt remains after a first-line diagnosis. Transcutaneous bone biopsy, which does not pass through the wound, is necessary to avoid contaminating the sample. This review focuses on the current diagnostic techniques for DFOs with an emphasis on the updates. To obtain the correct therapeutic results, selecting a proper option is necessary. Based on these numerous diagnosis modalities and indications, the proper choice of diagnostic tool can have favorable treatment outcomes.

• Reproductive and Developmental Biology
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• 제28권3호
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• pp.167-172
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• 2004

Flow cytometry를 이용하여 개 정자의 생존율 평가를 수행하고자 2-4세의 수캐 5두가 이용되었고, 분석을 위해 PI염색을 실시하였다. Flow cytometry를 이용한 개 신선 정액의 생존율 평가는 생존 정자와 죽은 정자의 비율을 1:0, 1:1, 1:3으로 조성하여 이를 flow cytometry로 평가하고 광학현미경검사, CFDA/PI 염색검사, HOS test에 의한 생존율과 비교하여 flow cytometry와의 상관관계를 알아보았다. 또한 개 정액을 동결하여 응해 후의 개 정자의 생존율 평가에도 동일한 방법으로 상관관계를 조사하였다. 신선 정액에서 생존 정자와 죽은 정자의 비율이 1:0, 1:1, 1:3 모든 경우에서 flow cytometry를 이용한 생존율은 HOS test에 의한 생존율과 높은 상관관계를 나타내었다 (p<0.01). 신선 정액에서 생존 정자와 죽은 정자의 비율이 1:0과 1:3일 때 광학현미경적 검사에 의한 생존율은 flow cytometry 분석에 의한 생존율과 유의 적인 상관관계를 나타내었으나 (p<0.05), 1:1 비율의 경우 상관관계를 보이지 않았다. 신선 정액에 생존 정자와 죽은 정자의 비율이 1:0과 1:1일 때 CFDA/PI 염색 검사에 의한 생존율은 flow cytometry분석에 의한 생존율과 높은 상관관계를 보였으며(p<0.01), 1:3 비율에서는 유의적인 상관관계를 보였다 (p<0.05). 동결 및 응해 후의 개 정자의 생존율 평가에서 HOS test 결과는 flow cytometry분석에 의한 생존율과 높은 상관관계를 보였으며 (p<0.01), 광학현미경적 검사를 통한 생존율은 유의적인 상관관계를 보였으나 (p<0.05), CFDA/PI 염색 검사결과는 상관관계를 보이지 않았다. 이상의 결과 flow cytometry는 신선정액 및 동결 융해 후 개 정자에 대한 생존율 검사에 정확한 평가 방법 인 것으로 판단되었다.

• 한국임상수의학회지
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• 제32권5호
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• pp.473-475
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• 2015

시골에 사는 12년령의 중성화 안한 수컷 말라뮤트가 회음부위의 염증과 통증을 주호소로 내원하였다. 회음부 신체검사에서 구더기와 구멍이 있는 피부 병변이 존재하였다. 혈액검사에서는 미약한 빈혈과 혈소판 증가증 이외에 특이소견은 보이지 않았으며 진단은 임상증상 및 구더기의 존재에 의해 쉽게 되었다. 즉시 삭모 및 소독을 실시하였으며 삭모 중 진드기가 발견되어 SNAP 4DX (IDEXX Laboratories, Westbrook, ME, USA)를 이용해 진드기 및 관련 병원체의 감염유무를 평가하였고 검사결과는 모두 음성이었다. 치료는 이버멕틴(300 mcg/kg)을 피하 주사로 한번 주사하였으며 2차 감염을 예방하기 위해 항생제를 처방하였다. 이 후 구더기증과 상처부위는 2주 이내에 치료되었다.

• 한국동물위생학회지
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• 제32권4호
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• pp.335-341
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• 2009

Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.

• 대한수의학회지
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• 제43권1호
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• pp.67-75
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• 2003

A total of 260 dogs were randomly selected from two different treed kennels that brucellosis has occurred (group 1, 126 dogs), and random selected breed kennel (group 2, 134 dogs), and monitored for Brucella canis (B. canis) by 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and bacterial culture method. For the differentiation, PCR-RFLP using omp-31, wbkA and per genes used for 52 of B canis strains (strain I) isolated in this study and 3 of B. canis strains (strain II) isolated in 1994 in Korea. 2ME-RSAT revealed that 63/126 dogs (50.0%) and 12/134 dogs (9.0%) were positive in group I and group II, respectively. Bacterial culture revealed that 47/126 dogs (37.3%) and 5/134 dogs (3.7%) were positive in group I and group II, respectively. As the results of PCR-RFLP, $\underline{omp}-31$ was amplified from all Brucella spp, except B. abortus. All B. canis isolates showed unique PCR-RFLP pattern following digestion with Bmel8I. However, all Brucella spp. showed the same PCR-RFLP pattern following digestion with SalI. PCR-RFLP analysis of wbkA revealed that all Brucella spp. showed the same pattern following digestion with HindIII. PCR-RFLP analysis of per revealed that B. abortus 544 and B. melitensis 63/9 showed the same pattern, but different from B. suis and B. canis following digestion with HindIII.

• Journal of Radiation Protection and Research
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• 제22권3호
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• pp.219-226
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• 1997

환경시료 및 Swipe시료에서 방출되는 감마선을 측정 분석하기 위하여 극저준위 백그라운드 감마선 측정시스템을 설치하였다. 본 측정시스템은 백그라운드를 저감하기 위해 극저방사능 물질로 구성된 수동적 차폐방법, 우주선 영향을 차단하기 위한 역동시 측정법, 그리고 공기중 방사성 핵종인 라돈가스를 시스템 내부에서 제거하는 방법 등을 적용하고 있다. 적용된 백그라운드 저감법에 대한 성능시험 결과, 실험실 조건의 백그라운드 준위가 관심 대상 에너지 영역($50\;keV{\sim}2\;MeV$)에서 $10^{-2}$ order 정도로 저감되었다. 그리고 미신고 핵활동을 탐지하기 위한 관심 대상 핵종의 감마선 에너지 영역에서 평가된 시스템의 최소검출하한(MDA)값은 환경시료 분석에 적합한 것으로 평가되었다. 그러나 저에너지 영역에서는 여전히 우주선에 의한 중성자 영향이 백그라운드 요인으로 작용하고 있는 것으로 분석되었다.

• Environmental Engineering Research
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• 제11권3호
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• pp.156-163
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• 2006

In this study, substrate removal characteristics were analyzed to reduce the cost of external carbon dosage at Sudokwon Landfill Site Management Corporation in Korea by utilizing oxygen uptake rate (OUR) and nitrate uptake rate (NUR) tests. To estimate and evaluate the substrate removal characteristics obtained by the batch tests, the lab-scale MLE process was operated. By-products of J Co. (sugar manufactory) and S Co. (fine chemical industry) were selected as the concerned carbon sources through a comparison of carbon and nitrogen contents. MeOH was tested as a control experiment. Until the steady state, the fraction of $RBDCOD_{OUR}$ concentration to COD concentration of J Co., S Co. by-products and MeOH increased and reached levels of 98%, 82%, and 100%, respectively. During the 20th operating day, the fraction of $RBDCOD_{NUR}$ concentration to COD concentration was 95%, 81%, and 83%, respectively. These fractions of $RBDCOD_{NUR}$ concentration to $RBDCOD_{OUR}$ concentration increased according to acclimation periods and reached levels of 99%, 97%, and 81%, respectively, on the 20th day. The results obtained from the lab-scale MLE process operation using the concerned carbon sources as external carbon were similar to that observed by OUR and NUR tests.

• 대한물리의학회지
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• 제17권1호
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• pp.49-61
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• 2022

PURPOSE: This study compared the effects of proprioceptive sensation, subacromial space, and dynamic function according to proprioceptive neuromuscular facilitation (PNF), static stretching (SS), and complex rotational stretching (CRS). METHODS: Thirty students without any musculoskeletal disease who volunteered to participate were included in this study. The following metrics were measured to evaluate the function and stability under the normal conditions, with the PNF, SS, and CRS: special test and flexion, extension, abduction, adduction, internal rotation, external rotation (shoulder range of motion) and reaching distance on the medial (ME), superolateral (SL), inferolateral (IL), and subacromial space and proprioceptive sensation were evaluated. All measures were analyzed using one-way ANOVA and repeated measures of ANOVA. RESULTS: A clear difference in adduction in the range of motion was observed in all groups (p < .05). Significant differences could not be identified in all values in the error test, except for Ab (p < .05). Significant differences in reach were noted in all directions of the SS and PNF in the Me, SL, and IL (p < .05). After the intervention, significant differences in the average values could be identified in all groups except for the SS group after rest (p < .05). After the intervention, there was a significant difference between the CRS and SS and PNF groups (p < .05). CONCLUSION: The application of CRS is as helpful as the existing SS and PNF for improving the joint range of motion improvement, shoulder balance, and subacromial space.

• 전기학회논문지
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• 제61권10호
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• pp.1448-1453
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• 2012

In this study, we carried out transient radiation test for identify failure situation by a transient radiation effect on operational amplifier devices. This experiments were carried out using a 60 MeV electron beam pulse of the LINAC(Linear Accelerator) facility in the Pohang Accelerator Laboratory. In this test, we has found that a serious failure as a burn-out effect due to overcurrent on the partial electronic devices.