• Title/Summary/Keyword: MDH isozyme

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척추동물의 Isozyme에 관한 비교연구: II. 개구리목 뇌조직의 Lactate 및 Malate Dehydrogenase Isozyme

  • 박상윤;조동현;고정식
    • The Korean Journal of Zoology
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    • v.15 no.3
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    • pp.105-110
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    • 1972
  • A cellulosse acetate electrophoretic survey of Korean Anura has revealed the presence of diverse lactate and malate dehydrogenase (LDH and MDH) isozymes. The pattern of LDH and MDH isozymes in the tissues of the central nervous system of the six species of Anura examined are species specific and differ from those of mammals and birds. Both Rana nigromaculata nigromaculata and Rana nigromaculata coreana have two molecular forms of LDH and MDH, respectively, with almost the same pattern. Whole brain homogenate of Rana temporaria shows also a maximum of only two LDH isozymes. Both Bufo bufo asiaticus and Bombina orientalis have five molecular forms of LDH with an entirely different spacing on the zymograms, whereas Rana rugosa has three. Two molecular forms of MDH are present in all animals examined and one band is shown in olfactory lobe and mixture of cerebellum and medulla oblongata of Rana nigromaculata nigromaculata.

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Changes of the Malate Dehydrogenase Isozymes in Oyster (Crassostrea gigas) Exposed to Different Temperature, pH and Salinity (온도, pH 및 염도가 굴(Crassostrea gigas)의 MDH isozyme에 미치는 영향)

  • 김지식;김종환
    • Korean Journal of Environmental Biology
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    • v.17 no.2
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    • pp.209-215
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    • 1999
  • Changes of malate dehydrogenase isozyme in oyster exposed to different temperature, pH and salinity were investigated by polyacrylamide gel electrophoresis. MDH isozyme in control group was separated into two bands on the positive side. In case of temperature and pH stress, MDH isozyme was separated into only one band after 12 hours exposure but two bands after 24, 48 hours exposure on the positive side. In case of salinity stress, after 12 hours exposure, MDH isozyme bands were separated into two bands in 5 ppt, 30 ppt and three bands in 10 ppt, 40 ppt concentration on the positive side. After 24 hours and 48 hours exposure case in salinity stress, MDH isozyme bands was separated into two bands on the positive side in all concentration. Activities of isozyme bands show their characteristics according to the condition of experiment. In conclusion, changes of MDH isozyme was a biochemical defense mechanism in oyster and result from effect of environmental stress to oyster.

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척추동물의 Isozyme에 관한 비교연구: III. 한국산 뱀목의 Lactate 및 Malate Dehydrogenase Isozyme

  • 김순옥;조동현;박상윤
    • The Korean Journal of Zoology
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    • v.16 no.1
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    • pp.55-65
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    • 1973
  • MDH and LDH isozymes of various tissues of serveral snakes which belong to Colubridae, Crotalidae and Viperidae were studied. MDH isozymes were appeared as two bands in all species. Four LDH isozymes were found inall species of Colubridae, Crotalidae and Viperidae. LDH isozymes of muscle and liver had fundamentally the same pattern. On the other hand, there were two LDH isozymes in the heart of all species and the pattern of heart LDH isozyme was reverse of the pattern in muscle. The pattern of LDH isozymes of stomach tissue can be thought as the same pattern with that of heart in all species, but in the stomach tissue of Crotalidae and Viperidae there were four LDH isozymes.

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척추동물의 Isozyme에 관한 비교연구: IV. 한국산 개구리목의 Lactate 및 Malate Dehydrogenase Isozyme

  • 고정식;조동현;박상윤
    • The Korean Journal of Zoology
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    • v.16 no.3
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    • pp.193-201
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    • 1973
  • A cellulose acetate electrophoretic survey of Korean Anura has revealed the presence of diverse lactate and malate dehydrogenase isozymes. The LDH and MDH isozymes in the tissues of the brain, heart, stomach, skeletal muscle and liver of the six species of Anura examined show the species specific patterns which differ from those of mammals and birds. Two isozymic forms of LDH and MDH exist in both Rana nigromaculata and Rana nigromaculata coreana, respectively, with almost the same pattern. LDH of Bombina orientalis has five isozymic forms, and that of Rana temporaria ornativentris contains four isozymes. Bufo sp. has 3 to 5, and Rana rugosa has 3 to 4 isozymic bands according to the tissues. MDH's of all animals have two isozymic forms with different spacing on the zymograms.

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Studies on the Change of Isozyme Patterns of Lactate and Malate Dehydrogenases During Embryonic Development of Some Amphibians (兩棲類 胚發生에 EK른 Lactate Dehydrogenase 및 Malate Dehydrogenase의 Isozyme 변화에 관하여)

  • Park, Young-Chul;Ha, Doo-Bong
    • The Korean Journal of Zoology
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    • v.23 no.4
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    • pp.263-272
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    • 1980
  • Polyacrylamide gel electrophoresis was used to investigate the patterns of LDH and MDH isozymes in the embryo and adult of amphibia; Rana nigromaculata, Rana plancyi chosenica and Hynobius leechii. Rana nigromaculata is considered to be heterozygous for the gene specifying the "B" subunit of LDH, and Hynobius leechii to be heterozygous for the gene specifying the "A" subunit of LDH. The LDH isozyme paatern of embryos of the above three species is characterized by a gradual increase in the activity of LDH-5 (muscular form)during development. Two or three molecular forms of MDH is present steadily from early embryos and in adult. Of the MDH isozymes, the more cathodic one (MDH-m) appears weakly in early developing stages, but increases slowly in the activity as the embryo develops.the embryo develops.

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Inheritance and Linkage of Some Polymorphic Isozymes in Ginkgo biloba L. (은행나무의 몇가지 다형적(多形的) 동위효소(同位酵素)의 유전양식(遺傳樣式) 및 연관(連關))

  • Kwon, Hae-Yeun;Kim, Zin-Suh
    • Journal of Korean Society of Forest Science
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    • v.89 no.4
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    • pp.527-535
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    • 2000
  • Isozyme variants of 15 enzyme systems were analyzed in megagametophytes of Ginkgo biloba L. Five enzyme systems (ADH, G6PD, IDH, MPI, and UGPP) appeared to be monomorphic. Only 11 isozyme zones observed in 10 enzyme systems were polymorphic : ACON-A, FST-B, GDH-A, GOT-B, MDH-B, MDH-C, MNR-A, PGI-B, PGM-A, 6PGD-B and SKDH-B. The segregation ratio and heterogeneity at most polymorphic zones suggested that each isozyme zone was controlled by a single locus with codominant alleles, but significant deviation from 1 : 1 segregation was observed at MDH-B in pooled data. Three pairs of isozyme loci (ACON-A : MDH-B, GOT-B : PGI-B, and MNR-A : SKDH-B) were found to be weakly linked. Recombination frequencies between them ranged from 0.38 to 0.40 (p<0.05).

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Isozyme Patterns at Five Loci Salmonids and Their Hybrid(I) (연어류에서의 종간교배 및 Isozyme Pattern에 관한 연구)

  • 홍경표;명정구;김병기;손진기
    • Journal of Embryo Transfer
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    • v.7 no.1
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    • pp.31-39
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    • 1992
  • 연어류의 종간교배 실험과 LDH, MDH, IDH, $\alpha$GODH, ME 등 다섯가지 isozyme에 대한 genetic marker로서의 활용 가능성을 알아보고자 1차적으로 연어, 산천어 및 무지개송어를 이용하여 종간교배를 실시하였고 이들 세종의 isozyme pattern을 비교하였다. 교배실험은 종간교배, 및 allotriploid 구간 등 12구간으로 나누어 실시하였으며 연어 암컷과 산천어 수컷의 교배결과가 초기성장 단계적에서 가장 우수하게 나타났고이들의 allotriploid도 부활율이 28.1%로 가장 우수하였다. Genetic identification을 위한 isozyme loci 분석결과 연어와 산천어는 대부분의 loci에서 거의 찾아볼 수 없었고 무지개송어는 MDH-B와 IDH에서 다형현상을 확인할 수 있었다. 특히 MDH-B loci는 b 유전자의 출현 빈도에서 세 종간의 식별이 가능하였으며, IDH pattern을 산천어와 무지개송어의 비교에 유효한 것으로 나타났다. 이들 두 loci는 hybrid의 genotype 분석시 유용한 marker로 활용할 수 있는 가능성을 보였고, 앞으로의 어류 육종에 좋은 기초 자료로 이용될 것으로 사료된다.

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Studies on the Isozyme Patterns Followed by Redifferentiation Stages of the Pepper(Capsicum annuum L.) - Changes of isozyme pattern on somatic embryogenesis in cutured pepper cells - (고추(Capsicum annuum L.)의 재분화 단계에 따른 Isozyme 양상에 관한 연구 - 고추 배양세포의 체세포배 형성에 따른 Isozyme 양상의 변화-)

  • 정현숙;공현진박진수강영희
    • KSBB Journal
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    • v.7 no.3
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    • pp.216-221
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    • 1992
  • The physiological changes of somatic embryogenesis in cultured pepper cells (Capsicum annuum L. cv Shinhong) were investigated. The somatic embryogenesis was induced by cultivating the callus in hormone-free MS medium. The peroxidase patterns in the somatic embryogenic cells and the culture medium was revealed three and two of cathodic and anodic bands by isoelectric focusing respectively. Activity of peroxidase released into culture medium was 4 times higher than that of 12th day cultured cells. At the heart stage, the isozyme patterns of the MDH and esterase were found to be changed, which were showed by starch gel electrophoresis. It means these isozymes can be used as markers for studying somatic embryogenesis and differentiation.

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Genetic Variation of the Pleurotus ostreatus Complex Based on Isozyme Analysis (동위효소 분석에 의한 Pleurotus ostreatus Complex의 유전적 변이)

  • Lee, Hee-Kyung;Yoo, Young-Bok;Min, Kyung-Hee
    • The Korean Journal of Mycology
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    • v.27 no.5 s.92
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    • pp.328-336
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    • 1999
  • Isozyme comparisons of mycelial extracts from Pleurotus ostreatus were undertaken using isoelectric focusing. Enzyme isozyme patterns were Used to describe the extent of geographical diversity and degree of intraspecific variation in these extracts. A total of 77 bands were resolved from six different enzymes. Cluster analyses were performed using the zymograms for esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), malate dehydrogenase(MDH), peroxidase (POX), and phosphoglucomutase (pGM). EST gave multiple banding patterns, while less variability was observed for GPI, MDH, and PGM. Cluster analyses demonstrated that strains of P. ostreatus from geographically different origins are genetically divergent, supporting the idea that there is little or no gene flow between these geographically distant population groups.

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