• 대한임상검사과학회지
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• 제37권1호
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• pp.41-46
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• 2005

It is known that mast cells (MCs) are increased in H. pylori-infected gastritis and its increase is mediated by stem cell factor (c-kit ligand). To determine the mechanism of mast cell recruitment and activation by stem cell factor, weinvestigated the expression of stem cell factor receptor (c-kit) in H. pylori-positive and -negative gastric mucosa. Biopsy specimens from 16 H. pylori-negative and 20 positive subjects were examined. H. pylori infection in gastric mucosa was examined by the Warthin-Starry method. MC and c-kit were identified by immunohistochemisty, using a monoclonal antihuman MC tryptase antibody and a polyclonal anti-human c-kit antibody. Densities of MC and c-kit positive cell were measured by a computerized image analysis system. MCs were detected in the lamina propria of both H. pylori-positive and -negative gastric mucosa. Densities of MC and c-kit positive cell were significantly greater in H. pylori-positive than -negative subjects. c-kit was located on the surface of MCs. These results indicate that stem cell factors may be one of the factors involved in mast cell increase and that they activate mast cells by binding with c-kit.

• 생명과학회지
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• 제23권1호
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• pp.102-109
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• 2013

전통적인 방법으로 제조된 청국장으로부터 우수한 발효 균주들을 분리하여 재래식으로 청국장을 제조하고, 제조된 청국장의 생리활성 물질인 GABA를 유지 및 보강하면서 품질을 향상시키기 위하여 청국장 발효능이 뛰어난 종균을 찾아 분리하였다. 분리된 균주들 가운데 GABA의 함량이 높은 청국장을 생산하는 MC 31을 실험균주로 선택하였고 API Kit와 16S rDNA sequence를 통하여 Bacillus subtilis MC 31로 명명하였다. B. subtilis MC 31의 최적배지와 온도, 시간을 찾아본 결과 LB 배지에서 $37^{\circ}C$, 24시간이 가장 높은 생육을 나타내었다. GABA 생산에 적합한 발효 온도와 시간을 조절하여 최적 조건을 찾아본 결과 B. subtilis MC 31는 $40^{\circ}C$에서 72시간에 가장 많은 GABA를 생산하였다.

• Journal of Animal Science and Technology
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• 제46권5호
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• pp.735-742
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• 2004

본 연구는 돼지의 품종특이 DNA marker를 이용하여 Large White, Landrace, Duroc의 순종 판별을 가능하게 하기 위해서 실시하였다 순종 판별을 위해 현재 알려져 있는 KIT과 돼지내의 모색과 밀접한 연관성이 있는 MCIR 그리고 mitrochondrial DNA상에서 종 특이적인 현상을 보이는 D-loop 지역의 11-bp 중복과 ND2 유전자의 개시 codon 변이를 이용하였다 품종간의 판별을 위해 KIT 유전자 exon17의 splicing 지역 변이를 활용하여 백색종과 유색종을 분류 하였다. MCIR 유전자의 (N121D)변이를 이용하여 유색종들로부터 Duroc 종이 분류되었다. 그러나 Duroc 이외의 유색종들 간에는 특이한 변이가 발견되지 않아 이 이상의 분류는 불가능 하였다 D-loop 지역의 11-bp 중복현상과 ND2 개시 codon의 변이에 의해 백색종인 Landrace(11-bp비중복과 ATT)종과 Large White(llbp 중복과 ATA) 종을 분류할 수 있었다. 결론적으로 본 연구에서 설정된 판별방법을 통하여 Landrace, Large White와 Duroc 종의 순종 판별이 완벽히 가능함을 입증하였다.

• 한국동물위생학회지
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• 제30권4호
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• pp.557-562
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• 2007

This study was carried out to discriminate Hanwoo from the milking and hybrid cattle by detection of MC1R gene related to bovine hair color. One hundred sixty six samples were collected from the abattoir (n = 106) and local market (n = 60). The beef from abattoir were originated from Hanwoo (n=27), Holstein (n=29), Hybrid (n=45) and imported cattle (n=5), respectively. The beef from market consisted of Hanwoo (n=36), Holstein (n=7) and imported ones (n=17). Commercialized screening kit (Kogenebiotec, Korea) was used for MC1R gene analysis. As a result, Hanwoo was discriminated from Holstein. However, 9 of 45 hybrid and 11 of 22 imported beef samples were indistinguishable from Hanwoo. It could be explained by second generation of crossing of Hanwoo with Holstein or the cattle with silver or yellow hair. This results suggest that additional tests as well as MC1R gene detection be needed to confirm Hanwoo beef among cattle beef.

• Journal of Periodontal and Implant Science
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• 제37권1호
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• pp.115-124
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• 2007

Silica is known as a promising osteoconductive material, and polycaprolactone is a bioactive and degradable material. The purpose of this study was to monitor the differentiation of MC3T3-E1 cells cultured on the layer-built silica/poly caprolactone non-woven fabric produced by electrospinning. Non-woven fabric (silica, polycaprolactone, PSP, SPS) was made by electrospinning and they were inserted in the 48 well cell culture plate. MC3T3-E1 cells were prepared by subculture. Cells were seeded to each well $1{\times}10^5$ concentration per well. Dulbecco's modified eagle medium with 10% FBS and 1% antibiotic-antimycotic solution was used. Confocal laser scanning microscope was taken 4 hours after incubation (95% air. 5% $CO_2$, $37^{\circ}C$). Cell proliferation was monitored by spectrophotometer on 1, 7, 14 days, and the morphology of the growing cells was observed by field emission scanning electron microscope. To monitor the differentiation of osteoblasts on the materials, MC3T3-E1 cells were incubated in 48 well culture plate after seeding with the density of $1{\times}10^5$ concentration. Then ELISA kit & EIA kit were used on to assess osteocalcin and osteopontin expression respectively. The other conditions were the same as above. MC3T3-E1 cells were proliferated well on all of the materials. There were no statistical differences among them. The osteopontin expression of silica, PSP, SPS was significantly higher than other groups on day 3 (p/0,05), but after that time, there were no statistically signigicant differences. The osteocalcin expression was significantly higher in silica and PSP than other groups on day 14. These findings show that PSP was as good as silica on the effect of osteoblast differentiation. The PSP non-woven fabric may have the possibility as bone graft materials.

• 생명과학회지
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• 제19권6호
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• pp.793-798
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• 2009

본 연구는 제주마에서 빈번하게 관찰되는 전신성 백모색 발생의 유전 양상과 유전적 변이와의 상관을 구명하기 위해 수행하였다. 백모색은 표현형과 MC1R 유전자형 분석 자료의 조합을 근거로 결정한 가라, 유마, 적다 등 모든 기본 모색에서 관찰되었다. 제주마에서는 타 품종들에서 KIT 유전자의 이형 접합성에 의해 발생하는 선천성 백색에 대한 잠재적 돌연변이 들은 발견되지 않았다. STX17 유전자의 intron 6 에서 4.6-kb 중복을 보유한 개체들에서 특이적으로 탈색된 백모색이 관찰되었다. 관찰기록과 STX17 유전자형에 따라 제주마에서 관찰되는 탈색된 백화현상은 총마(점진적 백화증, Gray) 로 확인되었다. 가계도 분석에서 총마 형질은 상동염색체성 우성유전형 질로 나타났으며 상동염색체성 열성형질인 albinism과도 구분되었다. 제주마에서 총마 모색이 자마 시기에는 명확하게 발현되는 않으며, 종종 다른 표현형들과 혼동을 일으키기도 하기 때문에, 총마와 이와 유사한 표현형으로 출생 시부터 혼합 모색을 나타내는 조모색, 상처 치료 후 백화, 백반 유사피부 백색증 등에 대한 추가 연구가 요구된다고 하겠다. 그럼에도 불구하고 총마와 유전적 배경의 관계를 구명한 본 연구결과는 제주마에서 분자육종을 위한 유용한 정보를 제공할 것으로 사료된다.

• Journal of Korean Neurosurgical Society
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• 제49권1호
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• pp.13-19
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• 2011

Objective: The purpose of this study is to investigate the combined effects of ginkgo biloba extract, ginkgolide A and B and aspirin on SK-N-MC, human neuroblastoma cell viability and mRNA expression of growth associated protein43 (GAP43), Microtubule-associated protein 2 (MAP2), B-cell lymphoma2 (Bcl2) and protein53 (p53) gene in hypoxia and reperfusion condition. Methods: SK-N-MC cells were cultured with Dulbecco's Modified Eagle's Medium (DMEM) media in $37^{\circ}C$, 5% $CO_2$ incubator. The cells were cultured for 8 hours in non-glucose media and hypoxic condition and for 12 hours in normal media and $O_2$ concentration. Cell survival rate was measured with Cell Counting Kit-8 (CCK-8) reagent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate mRNA levels of GAP43, MAP2, Bcl2, and p53 genes. Results: The ginkgolide A and B increased viable cell number decreased in hypoxic and reperfused condition. The co-treatment of ginkgolide B with aspirin also increased the number of viable cells, however, there was no additive effect. Although there was no increase of mRNA expression of GAP43, MAP2, and Bcl2 in SK-N-MC cells with individual treatment of ginkgolide A, B or aspirin in hypoxic and reperfused condition, the co-treatment of ginkgolide A or B with aspirin significantly increased GAP43 and Bcl2 mRNA levels. In MAP2, only the co-treatment of ginkgolide A and aspirin showed increasing effect. The mRNA expression of p53 had no change in all treating conditions. Conclusion: This study suggests that the combined treatments of Ginkgo biloba extracts and aspirin increase the regeneration of neuroblastoma cells injured by hypoxia and reperfusion.

• 드라이브 ㆍ 컨트롤
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• 제21권1호
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• pp.31-37
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• 2024

In this study, to prevent accidents in underground facilities during excavation, we developed a Lv.3 automated control system that can be configured as an electronic control system without changing the existing hydraulic system in a general excavator and utilized digital map information of underground facilities. We aimed to develop a strategy to prevent accidents caused by operator error. To implement this, a real-time excavator bucket end position recognition and control system was developed through angle measurement of the boom, arm, and bucket using an electronic joystick, RTK-GPS, and angle sensors. In addition, excavators are large, machine-based equipment, and it is difficult to control overshoot due to inertia with feedback control using position recognition information of the bucket tip. Therefore, feed-forward control is used to calculate the moving speed of the bucket tip in real-time to determine the target position. We developed a technology that can converge and verified the performance of the developed system through actual vehicle installation and field tests.

• Restorative Dentistry and Endodontics
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• 제44권2호
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• pp.17.1-17.10
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• 2019

Objectives: Root resorption is an unexpected complication after replantation procedures. Combining anti-osteoclastic medicaments with retrograde root filling materials may avert this resorptive activity. The purpose of this study was to assess effects of a cathepsin K inhibitor with calcium silicate-based cements on osteoclastic activity. Methods: MC3T3-E1 cells were cultured for biocompatibility analyses. RAW 264.7 cells were cultured in the presence of the receptor activator of nuclear factor-kappa B and lipopolysaccharide, followed by treatment with Biodentine (BIOD) or ProRoot MTA with or without medicaments (Odanacatib [ODN], a cathepsin inhibitor and alendronate, a bisphosphonate). After drug treatment, the cell counting kit-8 assay and Alizarin red staining were performed to evaluate biocompatibility in MC3T3-E1 cells. Reverse-transcription polymerase chain reaction, tartrate-resistant acid phosphatase (TRAP) staining and enzyme-linked immunosorbent assays were performed in RAW 264.7 cells to determine the expression levels of inflammatory cytokines, interleukin $(IL)-1{\beta}$, IL-6, tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and prostaglandin E2 (PGE2). Data were analyzed by one-way analysis of variance and Tukey's post hoc test (p < 0.05). Results: Biocompatibility results showed that there were no significant differences among any of the groups. RAW 264.7 cells treated with BIOD and ODN showed the lowest levels of $TNF-{\alpha}$ and PGE2. Treatments with BIOD + ODN were more potent suppressors of inflammatory cytokine expression (p < 0.05). Conclusion: The cathepsin K inhibitor with calcium silicate-based cement inhibits osteoclastic activity. This may have clinical application in preventing inflammatory root resorption in replanted teeth.

• 한국환경생태학회지
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• 제25권5호
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• pp.658-665
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• 2011

제주도에서는 절멸된 것으로 간주되었던 멧돼지들이 최근 한라산 인근지역에서 발견되었다. 본 연구는 분자유전학적 실험기법을 바탕으로 한라산 멧돼지들이 가축돼지들과 이종교배된 것들인지를 조사하였다. 또한 동일 종내에서의 유전적 유연관계와 분자 성판별을 시험하였다. 가축돼지 품종들(Landrace, Large White, Berkshire, Hampshire, Duroc)과의 교배여부는 핵 DNA와 미토콘드리아 DNA에서 4 종류의 분자 표지인자(MC1R, KIT, 조절영역, ND2)를 적용하여 시험하였다. 야생멧돼지 집단의 모든 개체들이 동일한 mtDNA 조절영역 서열을 나타내었고, 그 서열들은 중국 동북부 재래돼지들과 동일하였으나 기존에 보고된 한반도 멧돼지의 서열들과는 다른 것으로 확인되었다. 이상의 연구결과는 한라산 멧돼지집단이 중국 재래돼지 품종들과 근연이면서, 기존에 연구되지 않았던 유전적 계통에서 유래한 것으로 사료된다. 분자 성판별 결과 수컷에 비해 암컷이 2 배 이상으로 확인되어, 한라산 야생멧돼지 집단이 팽창하고 있으며, 조절하지 않으면 집단 규모는 극적으로 증가할 것이다.