The objects of this study were to develop a new strain which has resistance to fowl typhoid, high performance in laying, and producing brown shell eggs favored by domestic consumers. Several White Leghorn (WL) breeds known as possessing genetic resistance to fowl typhoid and several brown shell egg breeds such as susceptible to the disease were used to produce the controlled strains with cross mating between the strains within the breeds and the experimental strains with crossbreeding between the breeds. The crossbred strains were Chungnong(CN) 21, 22, 23, 24, 25 and 26. The controlled strains were ISA brown CC, Hyline brown CC and Lohman brown CC. The survival rates were 99.95% for chicks of age 0∼l7 weeks and 91% for adult chickens of age 18∼72 weeks. There was no difference in survival rate between the crossbred and the controlled strains. The means of age of lst egg laying were 147 to 148 days and no difference was observed between the crossbred and the controlled. The egg Production rates of a9e 18∼72 weeks were 83.76% far the crossbred strains and 77.82% for the controlled strains, which is significantly higher in the crossbred than controlled strains by 6%. The numbers of the hen housed egg Production of age 18∼72 weeks were 292.33 eggs for the crossbred strains and 271.31 eggs fur the controlled strains. The difference of 21 eggs more produced by the crossbred than by the controlled was statistically significant. The mean egg weights of age 18∼72 weeks were 64.32g for the crossbred strains and 60.73g for the controlled strains, and the difference of 3.59g was statistically significant. The feed conversion rates during the age of 18 to 72 weeks were 2.297 for the crossbred strains and 2.454 for the controlled strains. The crossbreds were reduced feed consumption by 157g for 1kg of egg Production which was statistically significant. Haugh unit(H$.$u) at the age of 72 weeks were 82.20 for the crossbred strains and 77.82 for the controlled strains. The crossbred strains were superior quality by 4.38 H$.$u. There were no significant differences in the yolk color and the eggshell thickness between the crossbred strains and the controlled strains. The eggshell color of the crossbred strains was light brown which is the medium color of the white eggshell strains and the brown eggshell strains. The body weights at the ages of 8, 12, 18, 42 and 71 weeks were not different between the crossbred strains and the controlled strains.
'Daebak', a new cultivar of Grifola frondosa, was bred by mating two monokaryotic strains isolated from 'F14309' and 'GMGF44062' at the Mushroom Research Institute, Gyonggi-Do ARES in 2017. The optimum temperature for mycelial growth of 'Daebak' was $25^{\circ}C$ on PDA medium. In bottle cultivation, the culture period of 'Daebak' was 57 days, which was 2 days shorter than that of 'Cham' (control). The pinheading rate of 'Daebak' was 98.4%, which was 24.8% higher than that of 'Cham' and its CV (Coefficient of variation) was 0.6%, 5.3% lower than that of 'Cham'. Regarding the growth characteristics of 'Daebak', the diameter and thickness of the pileus were 27.7 mm and 1.73 mm, respectively, and the diameter and height of the fruiting bodies cluster were 132 mm and 87.2 mm, respectively. The pileus was thinner but the fruiting bodies cluster was larger than that of 'Cham'. Fruiting bodies weighed 139 g per 1,100 ml bottle of 'Daebak', which was 28% higher than that for 'Cham', with a CV of 2.5%, which was 6.2% lower than that of 'Cham'. The yield per 10,000 bottles (used for cultivation) of 'Daebak' was 1,376 kg, 70% higher than that of 'Cham', with a CV of 3.0% that was 11.5% lower than that of 'Cham'. With respect to physical characteristics, the strength and brittleness of the fruiting body of 'Daebak' was less than that of 'Cham'. When considering the period available for sale, the shelf life of 'Daebak' was 42 days, which was 6 days longer than that of 'Cham'.
Journal of the Korean Society of Food Science and Nutrition
/
v.44
no.10
/
pp.1484-1491
/
2015
The new cultivar Lentinula edodes, which is named 'Lentinula edodes GNA01', was bred by mating strains isolated from 'L26' and 'Kyoungwon9015' obtained from Sammyungjin Research Institute, Fujian, China. L. edodes GNA01 does not have stipes like L. edodes, although it generally has a similar spherical shape. Moisture and crude protein contents of L. edodes GNA01 were lower than those of L. edodes. Meanwhile, L. edodes GNA01 contained higher levels of crude ash, crude lipid, crude fiber, and carbohydrates than L. edodes. The ${\beta}$-carotene content ($19.05{\mu}g/100g$) of L. edodes GNA01 was about three times higher than that of L. edodes. In addition, vitamin D content ($118.53{\mu}g/100g$) of L. edodes GNA01 was more than twice that of L. edodes. L. edodes GNA01 was a good source of mineral elements, with K and Mg contents of 2,277.50 mg/100 g and 203.15 mg/100 g, respectively. The major fatty acids of L. edodes GNA01 were C16:0 and C18:2, and L. edodes GNA01 had the highest linoleic acid (C18:2) content of 1,087.66 mg/100 g. Total phenol content of L. edodes GNA01 was 12.52 mg GAE/g, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and ferric reducing antioxidant power (FRAP) values of L. edodes GNA01 were lower than those of L. edodes at all concentrations. However, DPPH radical scavenging activities and FRAP values of L. edodes GNA01 were above 80% and 0.9 at a concentration of 10 mg/mL, respectively.
Kim, J.H.;Park, B.Y.;Yoo, Y.M.;Cho, S.H.;Kim, Y.K.;Lee, J.M.;Yun, H.J.;Kim, K.N.
Journal of Animal Science and Technology
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v.44
no.6
/
pp.793-800
/
2002
The characteristics of carcass and meat yields of fattening pigs by production steps were investigated with Landrace (LL, n=41), Yorkshire (YY, n=33), and Duroc (DD, n=30), $F_1$ (LY, n=25), the Crossbred of LYD (n=48). Duroc had more weight loss in carcasses weight than that of the other breed(p<0.05). Yorkshire and $F_1$ had higher production weight in retail cut than that of the other pure or crossbred. Carcass from Landrace and $F_1$ were significantly longer in length than the other breed(p<0.05). $F_1$ produced wider carcass than the other breed. Carcass thickness at aitch bone was higher for $F_1$ and Crossbred than the other breed(p<0.05). Landrace, Yorkshire and $F_1$ produced more loin and tenderloin in weight than the other breed (p<0.05). Yorkshire and $F_1$ produced more picnic shoulder when compared to the other breed. The hind legs produced from Yorkshire and $F_1$ were higher in weight and the fore legs produced from the Crossbred were higher in weight. Duroc produced the lowest weight of belly among the breeds. The acceptance level of loin were extremely low for all breed. Landrace had the highest acceptance level for tenderloin. Yorkshire had the highest acceptance level for picnic shoulder and ham when evaluated by export standard of Japan. In conclusion, The introduction of pure breed and establishment of mating steps are necessary to produce highly accepted pork with high acceptance in carcass and meat yields.
This study was conducted to obtain more detailed information on the characteristics of body fat distribution, genetic variation and correlation of fat amount at different location in broiler type chicken. A total of 429 offsprings, produced by mating 13 sires with 52 dams of WPR, were used for this study. All experimental analyses were made on dressed carcasses of 8 weeks of age. The average live body weight was 1,856g in male and 1,483g in female, the proportions of each part to live body weight were higher in male group except skin and abdominal fat pad. The total body fat was more in male (40.76g), but fat rate of total body, skin, and abdomen to live body weight were significantly higher in female group. The fat percentages in each body location were significantly higher in the fatty group and in each sex. The rates of abdominal fat to total body fat were increased by fattening, while subcutaneous fat percentages were lowered. There were high correlations between body weight and fat deposit in each location. The genetic correlation coefficients of body weight to fat percentages of skinned carcass, skin, abdomen, and total body fat to live body weight were negative in both sexes, but those to total body fat, eviscerated carcass fat, and skin fat were positive. In conclusion, the fat weight and fat percentage in individual chickens became higher by fattening however, genetic correlation between live body weight and percentages total body fat to live body weight were so low(negative) that decreasing body fat could be possible without decreasing body weight.
Choi, Eun Sik;Bang, Min Hee;Kim, Ki Gon;Kwon, Jae Hyun;Chung, Ok Young;Sohn, Sea Hwan
Korean Journal of Poultry Science
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v.44
no.2
/
pp.123-134
/
2017
This study was conducted to establish new synthetic lines of Korean Native Chicken. We performed $5{\times}5$ diallel crossings with GPS lines of Korean Native Chicken for the selection of parent stock. The production traits including viability, body weight, age at first egg laying, egg weight, hen-day egg production, and hen-housed egg production were measured and analyzed for 25 crosses with 1,157 hens. The heterosis effects of these traits were also estimated. The results showed that the average survival rate during laying periods was 67.7% in the pure lines and 77.1% in the crosses. The 25 cross combinations were shown to be distinctly divided into three groups according to body weight; nine crosses in the high-weight group, 12 crosses in the medium-weight group and four crosses in the light-weight group. The average body weight at 12 weeks of age was $1,873.8{\pm}43.0g$ in the high group, $1,595.4{\pm}56.6g$ in the medium group and $1,152.7{\pm}24.7g$ in the light group, and $1,560.2{\pm}339.3g$ in the pure lines and $1,640.9{\pm}213.7g$ in the crosses. In terms of egg production performance, the age at first egg laying was $139.7{\pm}4.9$ days in the pure lines and $135.8{\pm}5.1$ days in the crosses. The average egg weight was $52.3{\pm}1.6g$ in the pure line and $53.0{\pm}1.6g$ in the crosses. The average hen-housed egg production from 20 to 40 weeks was $58.3{\pm}12.9$ eggs in the pure line and $69.2{\pm}10.4$ eggs in the crosses. The average heterosis effect was 16.9% for survival rate, 5.4% for body weight at 12 weeks, -2.7% for age at first egg laying, 1.3% for egg weight, and 14.3% for hen-day egg production. Generally, the heterosis effect for viability and egg production traits was higher than that for growth trait. On the basis of these results, it is expected that the selection of the HF combination, which had excellent growth performance, is the most desirable as the paternal strain, and the selection of GW and FW combinations, which had excellent laying performance, are preferable as the maternal strain.
Occasionally, We make mistake of discrimination of sex since a pair of small spots around tile abdomen of 9th abdominal of males and posterior spots of females of the silkworms larvae are found at almost same position. As already have reported, more larvae which have the small spots were found in Chinese race than the Japanese. This research has been conducted in order to grasp the rate of appearance of the small spotted larvae hereditarily when hybridize with different race (F$_1$) 1. According to the first report, average 17,8% of the small spotted larvae are found in the Japanese race and 57.0% in the Chinese, but my re-invested data which was carried out recently shows that average 13.6% in the former case and 37.8% in the latter. Those new data are supposed to be come to a conclusion that the figures are flexible but in any case high frequency of appearance of the larvae were found in the Chinese race than the Japanese (Reference Table 1 ) 2. Miscalculated ratio of the larvae from males to females are 1.2-2.6%, and from females to males are found 0.5%-0.9% in the Japanese race, In case of the Chinese race, 7.1-9.4% in tile former and 2.9-3.4% in the latter. Those data also shows that more of the larvae found in Chinese race. 3. The larvae of small spotted and non-spotted are also seperated when hlybridize with different mating types and races. 4. More small spotted larvae are seen when hybridize with less appeared of the small spots of females and males which have the small spots than more appeared of the small spots of females with none spotted of the males (Reference Table 2. C.G.)B.F.). 5. More larvae of the small spots and found in case of hybridize in a group of having small spots than none of the spots (H>E). 6. With the exception of the case (A.B), in the same type of cross breeding (C.D, E.F, G,H), more larvae of the smal1 spots are also found in case of hybridize with males of having the spots than none of the spots.
This study was conducted to investigate the meat quality characteristics of pork loin in 3 crossbred groups (30 pigs/group). The crossbreeds were LY female${\times}$D male, Y female${\times}$B male and L female${\times}$B male (L: Landrace, Y: Yorkshire, B: Berkshire, D: Duroc). Pork loins were collected from animals ($110{\times}120kg$ body weight) slaughtered in a commercial slaughter house and stored at $-3^{\circ}C$. Samples were analyzed for general composition, physico-chemical properties, meat and fat color, texture characteristics, sensory evaluation and fatty acid composition at 24 hrs postmortem, and TBARS (Thiobarbituric Acid Reactive Substances) values were determined at various storage times. The LYD pigs showed a higher back fat thickness than the YB and LB pigs. There were no significant differences in intramuscular fat, intermuscular fat, subcutaneous fat and springiness among the 3 crossbreeds. In addition, there were no significant differences in general composition, pH, drip loss, cooking loss and cooked sensory evaluation among the 3 crossbreeds (p>0.05). The shear force value of LB pork was significantly lower than other crossbreeds (p<0.05). Total heme pigment was significantly higher in YB pork compared to LYD and LB pork (p<0.05). The TBARS value of YB pork was significantly lower than that of LYD pork, except after the I day of storage (p>0.05). TBARS values increased significantly during storage for all three crossbreeds. The lightness (CIE L) of YB pork was significantly lower, while the redness (CIE a) was significantly higher in YB pork relative to the other crossbreeds. With regard to textural properties, the hardness and adhesiveness values were significantly lower in YB pork compared to LB pork. In fresh meat, the color of YB pork was significantly higher than the other breeds (p<0.05). The content of myristic acid (C14:0) was significantly lower, while the content of arachidonic acid (C20:4) was significantly higher in LB pork (p<0.05). These results show that the YB breed has the highest quality meat of the three crossbreeds.
Recently, the rapid increase and global spread of extended-spectrum $\beta-lactamase$ producing clinical isolates has become a serious problem. The incidence of extended-spectrum $\beta-lactamase$ producing clinical isolates of Escherichia coli in Korea and susceptibility to antimicrobial agents were investigated. Total 233 isolates of E. coli were obtained from urine from hospitalized patients in Guro hospital, Korea University in 2001. One hundred and eighty four isolates $(78.9\%)$ were resistant to ampicillin, 80 isolates $(34.3\%)$ were resistant to cephalothin, 93 isolates $(39.9\%)$ were resistant to gentamicin, and 64 isolates $(27.5\%)$ were resistant to norfloxacin. Among 233 isolates, 17 isolates $(7.3\%)$ were positive as determined by the double disk synergy test. When minimal inhibitory concentrations were assayed with additional 6 antimicrobial agents, 13 isolates $(76.5\%)$ were multi-drug resistant to at least four different class antimicrobial agents. Extended-spectrum $\beta-lactamase$ were characterized with isoelectric focusing gel electrophoresis and DNA sequencing. They were TEM-1 in 5 isolates, TEM-15 in 1 isolate, TEM-20 in 1 isolate, TEM-52 in 4 isolates, TEM-1 and AmpC in 2 isolates, TEM-1 and OXA-30 in 1 isolate, TEM-1 and OXA-33 in 1 isolate, TEM-1, CTX-M-3, and AmpC in 1 isolate, but SHV was not detected. Antimicrobial resistance genes were transferred to animal isolate of E. coli (CCARM No. 1203) by the filter mating method. Extended spectrum $\beta-lactamase$ producers studied in the current study have low correlation to each other as determined by random amplified polymorphic DNA and pulsed field gel electrophoresis. This is a contradictory result from the general hypothesis that extended-spectrum $\beta-lactamase$ producers in one hospital is a result from a clonal spread.
This study is to investigate the amount of biased estimates for heritability and genetic correlation according to data structure on marbling scores in Korean cattle. Breeding population with 5 generations were simulated by way of selection for carcass weight, Longissimus muscle area and latent values of marbling scores and random mating. Latent variables of marbling scores were categorized into five by the thresholds of 0, I, 2, and 3 SD(DSI) or seven by the thresholds of -2, -1, 0,1I, 2, and 3 SD(DS2). Variance components and genetic pararneters(Heritabilities and Genetic correlations) were estimated by restricted maximum likelihood on multivariate linear mixed animal models and by Gibbs sampling algorithms on multivariate threshold mixed animal models in DS1 and DS2. Simulation was performed for 10 replicates and averages and empirical standard deviation were calculated. Using REML, heritabilitis of marbling score were under-estimated as 0.315 and 0.462 on DS1 and DS2, respectively, with comparison of the pararneter(0.500). Otherwise, using Gibbs sampling in the multivariate threshold animal models, these estimates did not significantly differ to the parameter. Residual correlations of marbling score to other traits were reduced with comparing the parameters when using REML algorithm with assuming linear and normal distribution. This would be due to loss of information and therefore, reduced variation on marbling score. As concluding, genetic variation of marbling would be well defined if liability concepts were adopted on marbling score and implemented threshold mixed model on genetic parameter estimation in Korean cattle.
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