• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1170-1178
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• 2008

The cyclopentenone prostaglandins (cyPGs) prostaglandin $A_1$ ($PGA_1$) and 15-deoxy-${\Delta}^{12,14}$-prostaglandin $J_2$ (15d-$PGJ_2$) have been reported to exhibit antiinflammatory activity in activated monocytes/macrophages. However, the effects of these two cyPGs on the expression of cytokine genes may differ. In this study, we investigated the mechanism of action of $PGA_1$ in lipopolysaccharide (LPS)-induced expression of inter leu kin (IL)-10 mRNA in mouse peritoneal macrophages. 15d-$PGJ_2$ inhibited expression of LPS-induced IL-10, whereas $PGA_1$ increased LPS-induced IL-10 expression. This synergistic effect of $PGA_1$ on LPS-induced IL-10 expression reached a maximum as early as 2 h after simultaneous $PGA_1$ and LPS treatment ($PGA_1$/LPS), and did not require new protein synthesis. The synergistic effect of $PGA_1$ was inhibited by GW9662, a specific peroxisome proliferator-activated receptor ${\gamma}(PPAR{\gamma})$ antagonist, and Bay-11-7082, a NF-${\kappa}B$ inhibitor. The extracellular signal-regulated kinases (ERK) inhibitor PD98059 increased the expression of $PGA_1$/LPS-induced IL-10 mRNA, rather than inhibiting the IL-10 expression. Moreover, $PGA_1$ inhibited LPS-induced ERK phosphorylation. The synergistic effect of $PGA_1$ on LPS-induced IL-10 mRNA and protein production was inhibited by p38 inhibitor PD169316, and $PGA_1$ increased LPS-induced p38 phosphorylation. In the case of stress-activated protein kinase/c-Jun $NH_2$-terminal kinase (SAPK/JNK), the SAPK/JNK inhibitor SP600125 did not inhibit IL-10 mRNA synthesis but inhibited the production of IL-10 protein remarkably. These results suggest that the synergistic effect of $PGA_1$ on LPS-induced IL-10 expression is NF-${\kappa}B$-dependent and mediated by mitogen-activated protein (MAP) kinases, p38, and SAPK/JNK signaling pathways, and also associated with the $PPAR{\gamma}$ pathway. Our data may provide more insight into the diverse mechanisms of $PGA_1$ effects on the expression of cytokine genes.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.111-116
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• 2013

Background: Cigarette smoking is the major risk factor for development of lung cancer. Identification of effects of tobacco on airway gene expression may provide insight into the causes. This research aimed to compare gene expression of large airway epithelium cells in normal smokers (n=13) and non-smokers (n=9) in order to find genes which discriminate the two groups and assess cigarette smoking effects on large airway epithelium cells.Materials and Methods: Genes discriminating smokers from non-smokers were identified by applying a neural network clustering method, growing self-organizing maps (GSOM), to microarray data according to class discrimination scores. An index was computed based on differentiation between each mean of gene expression in the two groups. This clustering approach provided the possibility of comparing thousands of genes simultaneously. Results: The applied approach compared the mean of 7,129 genes in smokers and non-smokers simultaneously and classified the genes of large airway epithelium cells which had differently expressed in smokers comparing with non-smokers. Seven genes were identified which had the highest different expression in smokers compared with the non-smokers group: NQO1, H19, ALDH3A1, AKR1C1, ABHD2, GPX2 and ADH7. Most (NQO1, ALDH3A1, AKR1C1, H19 and GPX2) are known to be clinically notable in lung cancer studies. Furthermore, statistical discriminate analysis showed that these genes could classify samples in smokers and non-smokers correctly with 100% accuracy. With the performed GSOM map, other nodes with high average discriminate scores included genes with alterations strongly related to the lung cancer such as AKR1C3, CYP1B1, UCHL1 and AKR1B10. Conclusions: This clustering by comparing expression of thousands of genes at the same time revealed alteration in normal smokers. Most of the identified genes were strongly relevant to lung cancer in the existing literature. The genes may be utilized to identify smokers with increased risk for lung cancer. A large sample study is now recommended to determine relations between the genes ABHD2 and ADH7 and smoking.

• Asian-Australasian Journal of Animal Sciences
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• 제18권1호
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• pp.17-21
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• 2005

The effect of melatonin on in vitro embryo development and the expression of antioxidant enzyme gene in preimplantation porcine embryos was determined by modified semi-quantitative single cell RT-PCR. Porcine embryos derived from in vitro maturation /in vitro fertilization were cultured in 5% $CO_2$ and 20% $O_2$ at $37^{\circ}C$ in NCSU23 medium. Melatonin was added to medium at concentration of 1nM, 5 nM, and 10 nM. When treated with 1nM (39.0%) of melatonin, the developmental rate of embryos beyond the morula stage were higher than that of control group (31.0%) (p<0.05). Number of inner cell mass and tropectoderm cell in control (23.0${\pm}$0.5 and 17.3${\pm}$0.8), 1 nM (23.6${\pm}$0.6 and 19.0${\pm}$0.5), and 5 nM (23.3${\pm}$1.1 and 16.3${\pm}$0.8) treated with melatonin were higher than in 10 nM (20.0${\pm}$0.5 and 13.3${\pm}$0.8) treated with melatonin (p<0.05). To develop an mRNA phenotypic map for the expression of catalase, bax and caspase-3, single cell RT-PCR analysis were carried out in porcine IVM/IVF embryo. Catalase was detected in 0, 1 and 5 nM supplemented with melatonin, but bax and caspase-3 were detected in 10 nM treated with melatonin.

• 한국농림기상학회지
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• 제24권2호
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• pp.63-77
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• 2022

농업기상재해 조기경보시스템에서 모의되는 농장 규모 풍속 예측자료의 추정오차를 개선하기 위해, 농촌진흥청 농업기상관측망의 2020년 1~12월 풍속 관측자료와 해당 지점에 대한 조기경보시스템 모의 풍속을 이용하여, 87지점 일 8시간대(00, 03, 06 … 21시) 각각 풍속 추정오차를 종속변수로, 추정풍속을 독립변수로 하는 일차 회귀식(Y=aX+b)을 도출하였다. 상관계수가 0.5를 초과하였을 때는 회귀식을 풍속 보정식으로 활용하고, 상관계수가 0.5 이하일 때는 회귀식 대신 해당 지점 및 시간대의 ME를 보정값으로 대체하였다. 풍속 모형을 전국적으로 적용할 수 있도록 87지점×8개 시간의 회귀계수 a와 b, 상관계수 R과 ME 값으로 거리역산가중법으로 공간내삽하여 250m 격자해상도의 분포도를 제작하였다. 모형의 검증을 위하여 회귀계수 a와 b, 상관계수 R과 ME 공간내삽 분포도로 부터 농산촌 지역 13개 기상관측지점의 격자값을 추출하고, 13곳의 2019년 1~12월의 조기경보시스템 모의 풍속(00, 03, 06 … 21시)를 보정한 다음, 기존 추정 풍속과 함께 추정오차를 비교하였다. 검증 지점 풍속의 평균 ME는 0.68m/s에서 보정 후 0.45m/s로 감소하였으며, 평균 RMSE는 1.30m/s에서 1.05m/s로 감소하였다. 조기경보시스템의 풍속은 전 시간대에서 모두 과대 추정되고 있는데, 보정 기법을 적용한 후에는 15시 경을 제외하고 모두 과대추정 경향이 감소하여 ME가 약 33%, RMSE는 19.2% 더 개선되었다. 농업기상재해 조기경보시스템에서 농작물의 풍해 위험 판단은 일 8회의 풍속 평균값으로부터 도출된 일 최대순간풍속을 기반으로 하는데, 풍속의 과대모의 현상을 개선하여 강풍 위험 경보의 오보를 감소시킬 것으로 기대된다.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.23-27
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• 2010

Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.

• 대한소아치과학회지
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• 제29권2호
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• pp.243-254
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• 2002

소아치과 임상에 흔히 사용되는 3종의 금속재에 의한 인체 섬유모세포(HGF-1)를 이용하여 세포독성에 대한 세포적 및 분자적 접근을 시도하였다. 세포의 성장과 증식 및 세포사, 그리고 이들과 관련된 세포내 신호전달물질 발현의 변화를 보고자 하였고, 세포사는 screening한 후 세포사의 유형(necrosis/apoptosis)을 규명하고자 하였다. HGF-1은 DMEM으로 계대배양한 후 유지하였으며, 사용한 금속재는 stainless steel crown (R), 교정용 band(B), 교정용 wire(W)의 세 종류였다. HGF-1세포의 증식에 대하여 기본적으로 cell count를 하였고, 생존세포 count를 위하여 대조군에 대한 실험군의 상대증식도를 계산하였다. 세포수는 배양일이 지남에 따라 증가하여 7일째에 최고를 나타내었고, 11일째에는 감소하였으며, 대조군과 실험군 사이의 통계적 유의한 차이는 관찰되지 않았다(p>0.05). 대조군에 대한 실험군의 상대증식도 또한 유의한 차이는 없었다(p>0.05). 세포사에 대한 형태적 소견은 괴사(necrosis)를 나타내었다. PCNA lableing index에 대한 대조군과 실험군간에 유의한 차이는 없었다(p>0.05). 신호전달 관련 MAP kinase인 ERK1 및 ERK2, p38, JNK는 배양일의 경과에 따른 발현의 변화는 관찰되었으나, 대조군과 각 실험군 간에 차이는 관찰되지 않았다. 결론적으로 본 연구대상인 stainless steel crown, 교정용 band 와 wire, 세 종류의 소아 치과용 금속재는 단기간의 인체섬유모세포를 이용한 세포친화성 검정에 있어서 세포적, 분자적 위해 양상을 나타내지 않았다.

• 자원환경지질
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• 제14권2호
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• pp.77-91
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• 1981

An airborne magnetometer survey was carried out over an offshore area of about $200,000km^2$ from the southeastern, southern and western part of Korea. Detailed magnetic studies on the geological structure of the southern part of above area ($100,000km^2$) was accomplished. Residual aeromagnetic map was made in order to delineate magnetic provinces, magnetic lineaments and sedimentary basins by application of least square method using computer system. To determine the depth of the sedimentary basins pseudo-gravimetric method was applied. 1. The area studied is divided into four magnetic provinces for the purpose of interpretation on the basis of the magnetic maps. 2. Near shore area and its attached islands of southern part (fiirst and second magnetic province) can be regarded as being the extension from the land geology due to presentation of strong magnetic anomalies and shallow magnetic basements. 3. Magnetic lineament 1-1 is strong magnetic anomalous region which is presumably relevant to volcanic activities in Cretaceous. The depth of magnetic basement of the lineament was determined to 1,500 m. Negative magnetic anomalous zones B1-1 and B1-2 which represent Tertiary basins showed depth of magnetic basement 3 km and 4 km each. The latter can be interpreted as extension of the Taiwan basin which is consisted of Tertiary sediments. 4. Magnetic lineament 2-1 coincide with Rainan-Fukien massif running NE-SW direction. A lineament located in central part of magnetic lineament 2-1 is well connected with extension of Sobacksan anticlinal axis on land. Volcanic rocks in Gyongsang system concentrated along this lineament. 5. The characteristics of magnetic pattern in the southern Yellow sea basin of western part of Jeju island show weaker magnetic anomalies and deeper magnetic basements than first and second magnetic provinces indicating geological structure of this basin seems to be quite different from that of Jeju strait. 6. In southern part of Jeju island, smoother magnetic pattern develope southward. Maximum depth of magnetic basement in sedimentary basins BIV-1 and BIV-2 were determined down to 6,000 m increasing its thickness toward Taiwan up to 11,000 m in the shelf area off Taichung, Taiwan. Judging from the fact that hydrocarbon was founded in the Tertiary sediments of western coastal area of Taiwan, it can be expected that hydrocarbon will be existed in these sedimentary basins of southern part of Jeju island.

• 미생물학회지
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• 제23권2호
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• pp.138-146
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• 1985

포도당 이성화효소를 coding는 Bacillus licheniformis ATCC31667의 유전자를 Escherichia coli LE 392-6에 클로닝하였다. Bacillus lieheniformis 염색체 DNA를 분리하고 제한효소인 Pst I.HindIII, Sal 1, EcoR 1, BamH1으로 절단한 후 운반제 plasmid인 pBR332에 연결하고 포도당 이성화효소 negative인 E. coli LE 3926-6에 형질전환하였다. 이중 E채꺄 제한효소를 사용한 것만이 glucose isomerase positive로 전환되어 xylose를 유일 탄소원으로 하여 성장하였다. 이 제조합 plasmid를 제한효소로 처리하여 본 결과 4.1Kb의 Bacillus licheniformisdb전자가 옮겨 졌음을 확인했고 여기에 제한효소 HindII와 Puv II의 절단위치가 확인되어 제한요소 지도를 작정하였다. 이 재조합 plasmid pBGI6는 연속계대 10일 후에도 매우안정하게 유지되었다. 한편 포도당 이정화 효소의 안정을 측정하여 본 바 야생숙주에 비해 약 20배의 증가를 나타냈다.

• BMB Reports
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• 제45권5호
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• pp.287-292
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• 2012

FGF-2 is involved in cell survival, proliferation, apoptosis, and angiogenesis in a wide variety of cells. FRGRs, PI3K and MAP kinases are well known mediators of FGF signaling. Despite its known roles during many developmental processes, including osteogenesis, there are few known targets of FGF-2. In the present study, we identified Bcl2-A1 and Bcl-xL as two prominent targets involved in promoting cell survival. Pretreatment of ATDC5 cells with FGF-2 increased cell survival, while siRNAs specific for Bcl2-A1 and Bcl-xL compromised the anti-apoptotic effect of FGF-2, sensitized the cells to apoptosis triggered by TNF-${\alpha}$. Chemical inhibition of FGFR, NFkB, and PI3K activity by PD173074, pyrrolidine dithiocarbamate, and LY294002 respectively abrogated the FGF-2-mediated induction of Bcl2-A1 and Bcl-xL expression. Taken together, our data demonstrate that a subset of Bcl2 family proteins are the targets of FGF-2 signaling that promotes the survival of ATDC5 cells.

• 한국가금학회지
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• 제44권3호
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• pp.199-209
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• 2017

Mitogen-activated protein kinase (MAPK) signaling pathways play a key role in innate immunity, inflammation, cell proliferation, cell differentiation, and cell death. The main objective of this study was to investigate the expression level of candidate MAPK pathway genes in the intestinal mucosal layer of two genetically disparate chicken lines (Marek's disease-resistant line 6.3 and Marek's disease-susceptible line 7.2) induced with necrotic enteritis (NE). Using high-throughput RNA sequencing, we investigated 178 MAPK signaling pathway related genes that were significantly and differentially expressed between the intestinal mucosal layers of the NE-afflicted and control chickens. In total, 15 MAPK pathway genes were further measured by quantitative real-time PCR(qRT-PCR) and the results were consistent with the RNA-sequencing data. All 178 identified genes were annotated through Gene Ontology and mapped onto the KEGG chicken MAPK signaling pathway. Several key genes of the MAPK pathway, ERK1/2, JNK1-3, p38 MAPK, MAP2K1-4, $NF-{\kappa}B1/2$, c-Fos, AP-1, Jun-D, and Jun, were differentially expressed in the two chicken lines. Therefore, we believe that RNA sequencing and qRT-PCR analysis provide resourceful information for future studies on MAPK signaling of genetically disparate chicken lines in response to pathogens.