• Journal of Microbiology and Biotechnology
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• 제30권2호
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• pp.287-295
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• 2020

The differences between luminal microbiota (LM) and mucosal microbiota (MAM) were little known, especially in duodenum. In this study, LM and MAM in colon and duodenum of mice were investigated through 16S rRNA high-throughput sequencing. The lowest bacterial diversity and evenness were observed in duodenal LM (D_LM), followed by duodenal MAM (D_MAM). Meanwhile, the bacterial diversity and evenness were obviously increased in D_MAM than these in D_LM, while no significant difference was observed between colonic MAM (C_MAM) and colonic LM (C_LM). PCoA analysis also showed that bacterial communities of LM and MAM in duodenum were completely separated, while these in colon overlapped partly. The ratio of Firmicutes to Bacteroidetes (F/B) in D_MAM was significantly higher than that in D_LM. Lactobacillus was largely enriched and was the characteristic bacteria in D_LM. The characteristic bacteria in D_MAM were Turicibacter, Parasutterella, Marvinbryantia and Bifidobacterium, while in C_LM they were Ruminiclostridium_6, Ruminiclostridium_9, Ruminococcaceae_UCG_007 and Lachnospiraceae_UCG_010, and in C_MAM they were Lachnospiraceae_NK4A136, Mucispirillum, Alistipes, Ruminiclostridium and Odoribacter. The networks showed that more interactions existed in colonic microbiota (24 nodes and 74 edges) than in duodenal microbiota (17 nodes and 29 edges). The 16S rDNA function prediction results indicated that bigger differences of function exist between LM and MAM in duodenum than these in colon. In conclusion, microbiota from intestinal luminal content and mucosa were different both in colon and in duodenum, and bacteria in colon interacted with each other much more closely than those in duodenum.

• 방송공학회논문지
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• 제24권5호
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• pp.813-826
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• 2019

본 논문은 UHD 콘텐츠를 고품질 방송소재로 활용하기 위한 MAM(Media Asset Management) 구현에 관한 연구이다. 본 논문의 구현방법은 워크플로우, 메타데이터, 시스템 인터페이스 관련 작업을 콘텐츠관리기능과 송출워크플로우 기능으로 MAM 역할을 분리하여 코어 MAM과 MAM-Ex 구조로 이원화하였다. 본 논문에서 제안하는 방법을 통해 송출소재 관련 메타데이터 수정은 페이지 메뉴 방식을 적용했고, 소재 구조체 API는 템플릿 방식을 적용함으로써 콘텐츠 관리방식을 개선시켰다. 또한 UHD 소재 스토리지 및 컴포넌트 서버의 구성은 채널 간 구별없는 풀(pool) 방식으로 통합하여 방송소재 보호와 콘텐츠 이동을 최소화하여 UHD 송출자산의 보안성을 강화하였다.

• Journal of Mechanical Science and Technology
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• 제15권2호
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• pp.199-209
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• 2001

Modal analysis method (MAM) is introduced for the fully coupled structural dynamic problems. In this paper, the beam with active constrained layered damping (ACLD) treatment is considered as a representative problem. The ACLD beam consists of a viscoelastic layer that is sandwiched between the base beam structure and an active piezoelectric layer. The exact damped natural modes are spectrally formulated from a set of fully coupled dynamic equations of motion. The orthogonality property of the exact damped natural modes is then derived in a closed form to complete the modal analysis method. The accuracy of the present MAM is evaluated through some illustrative examples: the dynamic characteristics obtained by the present MAM are compared with the results by spectral element method (SEM) and finite element method (FEM). It is numerically proved that MAM solutions become identical to the accurate SEM solutions as the number of exact natural used in MAM is increased.

• Molecules and Cells
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• 제41권12호
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• pp.1000-1007
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• 2018

Mitochondria and endoplasmic reticulum (ER) are essential organelles in eukaryotic cells, which play key roles in various biological pathways. Mitochondria are responsible for ATP production, maintenance of $Ca^{2+}$ homeostasis and regulation of apoptosis, while ER is involved in protein folding, lipid metabolism as well as $Ca^{2+}$ homeostasis. These organelles have their own functions, but they also communicate via mitochondrial-associated ER membrane (MAM) to provide another level of regulations in energy production, lipid process, $Ca^{2+}$ buffering, and apoptosis. Hence, defects in MAM alter cell survival and death. Here, we review components forming the molecular junctions of MAM and how MAM regulates cellular functions. Furthermore, we discuss the effects of impaired ER-mitochondrial communication in various neurodegenerative diseases.

• 대한토목학회논문집
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• 제14권1호
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• pp.13-20
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• 1994

동적(動的) 해석(解析)에 널리 사용되는 Mode Superposition Method(MSM)에 있어서 몇개의 모우드를 포함하여 해석(解析) 하느냐 하는 문제는 공학적(工學的) 판단에 의지할 수 밖에 없는 문제이다. 합리적(合理的)이고 알맞은 모우드수를 결정(決定)하기란 어려운 과제이고, 또 대단히 중요(重要)한 문제이다. 이러한 문제점을 보완할 수 있는 한 방법으로 Mode Acceleration Method(MAM)가 발표(發表)되었으나 주파수(周波數) 영역(領域)에서는 그 수식(數式)이 너무 복잡(複雜)하고, 까다로와서 또 다른 단점(短點)이 대두되었다. 본(本) 연구(硏究)에서는 이 단점을 극복할 수 있는 실용적(實用的)으로 간편화된 Advanced Mode Acceleration Method(AMAM)을 유도하였다. 예제(例題) 해석(解析)을 통하여 AMAM이 MSM 및 MAM보다 우수한 해법(解法)이 됨을 입증할 수 있었다.

• Macromolecular Research
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• 제14권6호
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• pp.646-653
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• 2006

Highly hydrophilic, uniform, superparamagnetic and nontoxic maltotrionic acid (MA)-coated magnetite nano-particles (MAM) were prepared and characterized by TEM, DLS, XRD and VSM. MA was used to improve the biocompatibility, monodispersity and non-specific intracellular uptake of nanoparticles. Folic acid (FA) was subsequently conjugated to the MAM to preferentially target KB cells (cancer cells) that have folate receptors expressed on their surfaces and to facilitate nanoparticles in their transit across the cell membrane. Finally, fluorescence isothiocyanate (FITC) was added to the nanoparticles to visualize the nanoparticle internalization into KB cells. After the cells were cultured in a media containing the MAM and MAM-folate conjugate (FAMAM), the results of fluorescence and confocal microscopy showed that both types of nanoparticles were internalized into the cells. Nevertheless, the amount of FAMAM uptake was higher than that of MAM. This result indicated that nanoparticles modified with MA and FA could be used to facilitate the nanoparticle uptake to specific KB cells (cancer cells) for molecular imaging.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2000년도 봄 학술발표논문집 Vol.27 No.1 (B)
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• pp.580-582
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• 2000

본 논문에서는 MAM을 이용한 특징 벡터의 보정을 기반으로 하는 헤드 제스처 인식에 관해 기술한다. 제안된 시스템은 얼굴 움직임 검출 모듈과 눈 영역 추적 모듈, 미 측정된 벡터 보정 모듈, 측정된 제스처에 대한 인식모듈로 구성된다. 신경망과 모자이크 이미지를 이용하여 얼굴 영역을 검출하고, 이 영역에서 눈 영역을 검출한다. 만약 눈의 쌍이 검출되지 않는다면 시스템은 특징 벡터 보정(MAM)을 수행하여 손실된 정보를 예측한다. 검출된 눈 영역은 정규화된 벡터로 변경된다. 이 벡터의 분산을 이용하여 긍정, 부정, 중립의 제스처를 판단한다. 제스처의 인식은 직접 관측, 이중 HMM, 삼중 HMM을 사용한 다중 인식기를 이용한다.

• International Journal of Oral Biology
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• 제31권2호
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• pp.33-43
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• 2006

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs)on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injected control animals. Field potential recordings were used to monitor amplitude and numbers of population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5 mg/kg; phenytoin 40, 60 mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320 mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

• The Korean Journal of Physiology and Pharmacology
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• 제10권5호
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• pp.235-242
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• 2006

Cortical malformation-associated epileptic seizures are resistant to conventional anticonvulsant drugs. Relatively little research has been conducted on the effects of antiepileptic drugs (AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate (MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of ethosuximide (ETX) in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injected control animals. Field potential recordings were used to monitor the amplitude and number of population spikes, and paired pulse inhibition in response to stimulation of the commissural pathway. Pharmaco-resistance was tested by measuring seizure latencies after pilocarpine administration (320 mg/kg, Lp.) with and without pre-treatment with ETX. Pre-treatment with 300 mg of ETX significantly prolonged the latency to the status epilepticus (SE) in both control and MAM-treated groups. Pre-treatment with ETX 100mg and ETX 200 mg had little effect in MAMexposed rats. However, ETX 200 mg prolonged the latency to the SE in control groups. Spontaneous field potential and secondary after-discharges were higher for MAM-treated rat in comparison with control rats injects with ETX. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard ETX assessed in vivo. These data suggest that ETX do not prolong seizure latencies in MAM-rats exposed to pilocarpine.

• 미생물학회지
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• 제54권4호
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• pp.420-427
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• 2018

효모균 타이로실-tRNA 합성효소(Sc YRS)와 엠버 멈춤코돈을 인식하는 대장균 시작tRNA 변이체(fMam $tRNA_{CUA}$)쌍은 대장균에서 단백질 생합성시 원하는 특정 위치에 비천연아미노산을 도입하는데 활용된다. Sc YRS/fMam $tRNA_{CUA}$쌍의 엠버써프레션 활성을 높이기 위해 fMam $tRNA_{CUA}$의 첫번째 안티코돈 염기를 인식하는 Sc YRS의 320번, 321번 아미노산 잔기를 암호화하는 염기서열을 무작위로 돌연변이시킨 라이브러리를 제작하였다. 엠버써프레션에 의한 클로람페니콜 저항성을 이용해 라이브러리를 탐색하여 활성이 향상된 2개의 돌연변이주를 선별하였다. 이들의 클로람페니콜 저항성 성장의 $IC_{50}$값은 야생형 YRS보다 1.7~2.3배 높았으며, in vivo 엠버써프레션 활성을 비교한 결과 3~6.5배의 활성 증가가 나타났다. 높은 활성을 보인 mYRS-3 (P320A/D321A) 단백질의 fMam $tRNA_{CUA}$에 대한 in vitro aminoacylation kinetics 분석은 야생형보다 약 7배 높은 효소활성을 보였으며, 이는 주로 기질인 fMam $tRNA_{CUA}$에 대한 결합 친화도가 증가하여 나타났다. 이런 접근법을 이용하여 다양한 종류의 비천연 아미노산 도입에 활용되는 aminoacyl-tRNA 합성효소의 엠버써프레션 활성을 높임으로써 엠버 멈춤코돈을 이용한 비천연 아미노산 도입 효율성을 높일 수 있을 것이다.