• 제목/요약/키워드: MALDI MS

검색결과 333건 처리시간 0.03초

Effect of Uracil Addition of Proteomic Profiles and 1,3-Beta-Glucan Production in Agrobacterium sp.

  • Jin, Li-Hua;Lee, Jung-Heon
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2005년도 생물공학의 동향(XVII)
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    • pp.674-679
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    • 2005
  • With addition of uracil during fermentation, the production rate of $1,3-{\beta}-glucan(curdlan)$ was enhanced. Since uracil was used as precursor of UDP-Glucose, the UDP-glucose level was increased and further glucan synthesis rate was increased.

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Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis

  • Song, Hyun-Ouk
    • Parasites, Hosts and Diseases
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    • 제54권1호
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    • pp.71-74
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    • 2016
  • Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis.