• Title/Summary/Keyword: MACS

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Performance of R1234yf and R1234yf/R134a Mixture under Mobile Air-conditioner Operating Conditions (R1234yf와 R1234yf/R134a의 자동차 에어컨 작동 조건에서의 성능 평가)

  • Park, Ki-Jung;Lee, Yo-Han;Choe, Dae-Seong;Jung, Dong-Soo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.22 no.12
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    • pp.837-844
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    • 2010
  • In this study, performance of R1234yf and R1234yf/R134a mixture is measured on a heat pump bench tester in an attempt to substitute R134a used widely in mobile air conditioners (MACs). The bench tester is equipped with a open type compressor providing a nominal capacity of 3.5 kW. All tests are conducted under the summer cooling and winter heating conditions of 7/4 $5^{\circ}C$ and $-7/41^{\circ}C$ in the evaporator and condenser, respectively. For R1234yf/R134a mixture, measurements are made at 5%, 10%, and 15% of R134a by mass. Test results show that the coefficient of performance (COP) and capacity of R1234yf are up to 2.7% and 4.0% lower than those of R134a, respectively. For R1234yf/R134a mixture, the COP and capacity are up to 3.9% lower and 3.6% higher than those of R134a. For R1234yf and R1234yf/R134a mixture, the compressor discharge temperature is $4.1{\sim}6.7^{\circ}C$ lower than that of R134a while the amount of charge is reduced up to 11% as compared to R134a. 90%R1234yf/10%R134a is a better refrigerant than pure R1234yf in that it is less flammable and more compatible with existing R134a system. Based upon the results, it is concluded that R1234yf and R1234yf/R134a mixture are long term environmentally friendly solutions to mobile air-conditioners due to their excellent environmental properties with acceptable performance.

Study on the simplifying antibody cocktail technique for isolation of human mesenchymal stromal cells (hMSCs) (사람 Mesenchymal stromal cell(hMSC) 분리를 위한 간소화된 방법에 대한 연구)

  • Park, Jung-Hyun;Kim, Kyoung-Hwa;Lee, Yong-Moo;Ku, Young;Rhyu, In-Chul;Han, Soo-Boo;Chung, Chong-Pyoung
    • Journal of Periodontal and Implant Science
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    • v.34 no.1
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    • pp.93-100
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    • 2004
  • 많은 연구들에서 hMSC를 얻기 위해 centrifugation, fluoroscence activated cell sorter(FACS), magnetic activated cell sorter(MACS)가 이용되어져 왔다. 그러나 centrifugation만을 이용한 경우 순도가 떨어지며 FACS나 MACS의 경우에는 비용, 시간이 많이 드는 단점이 있다. 따라서 이 연구에서는 antibody cocktail을 이용하여 hMSC를 좀더 쉽게 얻어내는 방법에 대해 알아보았다. 사람의 골반에서 12G의 바늘을 이용하여 골수를 흡입한 후 heparin이 들어있는 시험관에 넣고 처리과정을 시행하기 전에 냉장고에 보관하며 가능한 한 빨리 처리 과정을 실시한다. 얻은 골수에 적당량의 RosetteSep( Stemcell Technologies)을 첨가한 후 실온에서 20분간 반응시킨다. 그 후 적당량의 Ficoll-paque위에 골수와 RosetteSep의 혼합물을 섞이지 않게 올리고 원심분리를 이용하여 원하는 세포층을 얻어낸다. 이 세포층을 따로 분리한 뒤 배양한다. 배양 시 세포가 80%이상 차기 전에 계속 passage를 시행하며 배양한다. 이는 세포가 밀도가 높아져 원치 않는 세포로 분화되는 것을 막기 위함이다. 배양된 세포가 다양한 분화능력을 가지고 있는지 알아보기 위해 세 가지로 분화를 유도하였다. 적절한 배지와 적절한 환경에서 배양함으로써 얻어진 세포를 osteoblast, chondroblast, adipocyte로 분화를 유도하였다. 분화된 세포가 원하는 형질의 세포로 분화되었는지를 확인하기 위하여 osteoblast의 경우 alizarin red staining, alkaline phosphatase activity, chondroblast의 경우 toluidine blue staining, adipocyte의 경우 Oil-Red-O staining으로 염색하여 분화를 확인하였다. 분리해낸 세포는 각각 세 가지 세포로 분화가 되었으며 이는 RosetteSep이 hMSC를 성공적으로 분리해냈다는 것을 보여준다. 그러나 모든 세포가 분화를 보이지는 않았으며 따라서 hMSC의 순도를 높이기 위한 연구가 더 필요하다. RosetteSep을 이용하면 다른 방법들 보다 쉽게 hMSC를 얻을 수 있으나 기존의 방법과 순도의 측면에서 더 비교할 필요가 있다.

Measurements of Mid-arm Circumference(MAC) and Mid-arm Circumference/Head Circumference (MAC/HC) Ratio as Indices of Nutritional Status in Newborn Infants (신생아 신체 발육지표로서의 중앙 상완위 및 중앙 상완위와 두위비의 측정)

  • Lee, Jae-Jun;Lee, Kyung-A;Lee, Young-Hwan;Shin, Son-Moon
    • Journal of Yeungnam Medical Science
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    • v.11 no.1
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    • pp.160-166
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    • 1994
  • Mid-arm circumference and mid-arm circumference/head circumference ratio(MAC/HC) were measured in 207 AGA(appropriate for gestational age) infants delivered at 26 to 42 weeks of gestation from January 1990 to December 1993 in Yeungnam University Hospital, Taegu, Korea. There were linear relationships between MACs and MAC/HC ratios and gestational age(MAC : y=0.3181x - 2.2069, r=0.81, p<0.001 ; MAC/HC ratio : y=0.0049x+0.1128, r=0.62, ; < 0.001). Using standard curves of MAC and MAC/HC ratio according to the gestational age, measurement of MAC or MAC/HC ratio can be a noninvasive, simple method to evaluate the intrauterine growth of newborn infants and the nutritional status of growing premature infants.

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Routing and Medium Access Control Interactions for Internet of Things (사물인터넷을 위한 라우팅과 매체접근제어의 상호작용)

  • Park, Pangun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.10
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    • pp.2465-2472
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    • 2015
  • Wireless Medium Access Control (MAC) and routing technologies are the basic building blocks making it possible the Internet of Things (IoT). These technologies have been the focus of substantial research in the last decade. Nevertheless, as new networking standards for IoT are being proposed and different solutions are patched together, evaluating the performance of the network becomes unclear. In this paper, a new overview of MAC and routing protocols for IoT and new interactions are given. The IEEE 802.15.4 MAC and Routing Protocol for Low-power Lossy Network (RPL) routing protocols are taken as reference to exemplify and illustrate the discussion. Experimental results show that contention-based access MACs may hurt the routing, unless these two are carefully designed together.

EEPB-MAC: Energy Efficient & Priority-Based MAC Protocol for Energy Harvesting Wireless Sensor Networks (에너지 수확 무선 센서 네트워크에서 에너지 효율 및 우선순위 기반의 MAC 프로토콜)

  • Kim, Seong-Cheol;Jeon, Jun-Heon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.3
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    • pp.755-761
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    • 2013
  • Medium access control (MAC) protocols for wireless sensor networks make sensor nodes on state for energy-efficiency only when necessary. In this paper we present an energy efficient priority-based MAC protocol for energy-harvesting Wireless Sensor Networks (WSNs). For support priority-based packet transmission the proposed EEPB-MAC protocol uses the modified IEEE 802.15.4 beacon frames including priority bit, sender node address, and NAV value fields. A receiver node periodically wakes up, receives sender beacon frames, selects data sending sender, and broadcasts a beacon frame containing the selected sender's address. A receiver node selects sender node according to sender's data priority. A receiver nodes also adjust wake up period based on their energy states. Hence, the energy consumption of receiver node can be minimized. Through simulations and analytical analysis, we evaluate the performance of our proposed the EEPB-MAC protocol and compare it against the previous MACs. Results have shown that our protocol outperforms other MAC in terms of energy consumption, higher priority packet delivery delay.

A Novel Implementation of Fault-Tolerant Ethernet NIC (Network Interface Card) Using Single MAC (단일 MAC을 이용한 자동 고장 극복 Ethernet NIC (Network Interface Card) 장치 구현)

  • Kim, Se-Mog;Pham, Hoang-Anh;Lee, Dong-Ho;Rhee, Jong Myung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37C no.11
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    • pp.1162-1169
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    • 2012
  • One of the important operational requirements for mission critical Ethernet networked system is having the fault tolerant capability. Such capability can be obtained by equipping multiport Network Interface Card (NIC) in each node in the system. Conventional NIC uses two or more Media Access Controls (MACs) and a co-processor for the MAC switching whenever an active port fails. Since firmware is needed for the co-processor, longer fail-over switching and degraded throughput can be generally expected. Furthermore the system upgrading requiring the firmware revision in each tactical node demands high cost. In this paper we propose a novel single MAC based NIC that does not use a co-processor, but just use general discrete building blocks such as MAC chip and switching chip, which results in better performances than conventional method. Experimental results validate our scheme.

Security Proof for a Leakage-Resilient Authenticated Key Establishment Protocol

  • Shin, Seong-Han;Kazukuni Kobara;Hideki Imai
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.14 no.4
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    • pp.75-90
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    • 2004
  • At Asiacrypt 2003, Shin et al., have proposed a new class for Authenticated Key Establishment (AKE) protocol named Leakage-Resilient AKE ${(LR-AKE)}^{[1]}$. The authenticity of LR-AKE is based on a user's password and his/her stored secrets in both client side and server side. In their LR-AKE protocol, no TRM(Tamper Resistant Modules) is required and leakage of the stored secrets from $.$my side does not reveal my critical information on the password. This property is useful when the following situation is considered :(1) Stored secrets may leak out ;(2) A user communicates with a lot of servers ;(3) A user remembers only one password. The other AKE protocols, such as SSL/TLS and SSH (based or PKI), Password-Authenticated Key Exchange (PAKE) and Threshold-PAKE (T-PAKE), do not satisfy that property under the above-mentioned situation since their stored secrets (or, verification data on password) in either the client or the servers contain enough information to succeed in retrieving the relatively short password with off-line exhaustive search. As of now, the LR-AKE protocol is the currently horn solution. In this paper, we prove its security of the LR-AKE protocol in the standard model. Our security analysis shows that the LR-AKE Protocol is provably secure under the assumptions that DDH (Decisional Diffie-Hellman) problem is hard and MACs are selectively unforgeable against partially chosen message attacks (which is a weaker notion than being existentially unforgeable against chosen message attacks).

ChIP-seq Analysis of Histone H3K27ac and H3K27me3 Showing Different Distribution Patterns in Chromatin

  • Kang, Jin;Kim, AeRi
    • Biomedical Science Letters
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    • v.28 no.2
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    • pp.109-119
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    • 2022
  • Histone proteins can be modified by the addition of acetyl group or methyl group to specific amino acids. The modifications have different distribution patterns in chromatin. Recently, histone modifications are studied based on ChIP-seq data, which requires reasonable analysis of sequencing data depending on their distribution patterns. Here we have analyzed histone H3K27ac and H3K27me3 ChIP-seq data and it showed that the H3K27ac is enriched at narrow regions while H3K27me3 distributes broadly. To properly analyze the ChIP-seq data, we called peaks for H3K27ac and H3K27me3 using MACS2 (narrow option and broad option) and SICER methods, and compared propriety of the peaks using signal-to-background ratio. As results, H3K27ac-enriched regions were well identified by both methods while H3K27me3 peaks were properly identified by SICER, which indicates that peak calling method is more critical for histone modifications distributed broadly. When ChIP-seq data were compared in different sequencing depth (15, 30, 60, 120 M), high sequencing depth caused high false-positive rate in H3K27ac peak calling, but it reflected more properly the broad distribution pattern of H3K27me3. These results suggest that sequencing depth affects peak calling from ChIP-seq data and high sequencing depth is required for H3K27me3. Taken together, peak calling tool and sequencing depth should be chosen depending on the distribution pattern of histone modification in ChIP-seq analysis.

Influence of Snow Accumulation and Snowmelt Using NWS-PC Model in Rainfall-runoff Simulation (NWS-PC 모형을 이용한 강우-유출 모의에서 적설 및 융설 영향)

  • Kang, Shin Uk;Rieu, Seung Yup
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.1B
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    • pp.1-9
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    • 2008
  • The impact of snow accumulation and snowmelt in rainfall-runoff modelling was analyzed for the Soyanggang dam basin by comparing the measured and simulated discharges simulated by the NWS-PC model. Sugawara's conceptual model was used to simulate the snow accumulation and snowmelt phenomena and NWS-PC model was employed to simulate rainfall-runoff. Parameters in model calibration were estimated by the Multi-step Automated Calibration Scheme and optimized using SCE-UA algorithm in each step. The results of the model calibration and verification show that the model considering snowmelt process is better than the one without consideration of snowmelt under the performance criteria such as RMSE, PBIAS, NSE, and PME. The measured discharge time series has over 60 days of persistence. Correlograms for each simulation showed that the simulated discharge with snowmelt model reproduce the persistence closely to the measured discharge's while the one without snow accumulation and snowmelt model reproduce only 20 days of persistence. The study result indicates that the inclusion of snow accumulation and snowmelt model is important for the accurate simulation of rainfall-runoff phenomena in the Soyanggang dam basin.

The Effect of Simple Freezing Method on Viability of Frozen-thawed Primordial Germ Cells on the Chicken (간이 동결 방법이 닭 원시 생식 세포의 생존율에 미치는 영향)

  • Kim, Hyun;Cho, Young Moo;Han, Jae Yong;Choi, Sung Bok;Cho, Chang-Yeon;Suh, Sangwon;Ko, Yeoung-Gyu;Seong, Hwan-Hoo;Kim, Sung Woo
    • Korean Journal of Poultry Science
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    • v.41 no.4
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    • pp.261-270
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    • 2014
  • This study was conducted to establish the method for preserving chicken primordial germ cells (PGCs) that enables long-term storage in liquid nitrogen ($LN_2$) for developmental engineering or preservation of species. The purpose of this study is to clarify the effects of simple freeze-thaw treatment on viability of PGCs in chickens and to the optimal protocol for PGCs freezing. PGCs obtained from the germinal gonade of an early embryos of 5.5~6 day (stage 28) of Isa Brown, Korean Ogye (KO), White Leghorn and Commercial breeds, using the MACS method were suspended in a freezing medium containing a freezing and protecting agents (e.g. dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PG)). The gonadal cells, including PGCs, were then frozen in 1 of the following cryoprotectant treatments : 2.5%, 5%, 10%, 15%, and 0% cryoprotectant (DMSO, EG, PG) as a control. Effects of exposure to simple freezing, with different concentrations of the cryoprotectant solution, were examined. After simple freezing, the viability of PGCs after freeze-thawing was significantly higher for Commercial breeds ($88.7{\pm}2.4%$) than KO ($85.1{\pm}0.4%$), Isa Brown ($84.6{\pm}0.2%$) and White Leghorn ($85.9{\pm}0.1%$) (p<0.05) using 10% EG cryoprotectant. Therefore, these systems may contribute in the improvement of cryopreservation for a scarce species in birds preservation. This study established a method for preserving chicken PGCs that enables systematic storage and labeling of cryopreserved PGCs in liquid ($LN_2$) at a germplasm repository and ease of entry into a database.