• BMB Reports
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• v.48 no.10
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• pp.583-588
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• 2015

Microtubule actin crosslinking factor 1 (MACF1), a widely expressed cytoskeletal linker, plays important roles in various cells by regulating cytoskeleton dynamics. However, its role in osteoblastic cells is not well understood. Based on our previous findings that the association of MACF1 with F-actin and microtubules in osteoblast-like cells was altered under magnetic force conditions, here, by adopting a stable MACF1-knockdown MC3T3-E1 osteoblastic cell line, we found that MACF1 knockdown induced large cells with a binuclear/multinuclear structure. Further, immunofluorescence staining showed disorganization of F-actin and microtubules in MACF1-knockdown cells. Cell counting revealed significant decrease of cell proliferation and cell cycle analysis showed an S phase cell cycle arrest in MACF1-knockdown cells. Moreover and interestingly, MACF1 knockdown showed a potential effect on cellular MTT reduction activity and mitochondrial content, suggesting an impact on cellular metabolic activity. These results together indicate an important role of MACF1 in regulating osteoblastic cell morphology and function.

• Journal of Life Science
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• v.22 no.12
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• pp.1651-1657
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• 2012

Plectin and microtubule actin cross-linking factor 1 (MACF1) are architectural proteins that contribute to the function of skeletal muscle as generators of mechanical force. However, the influence of insulin- like growth factor-I (IGF-I), a master regulator of skeletal muscle cells, on plectin and MACF1 in skeletal muscle cells has not been demonstrated. The effect of IGF-I on plectin and MACF1 gene expression was investigated by treating differentiated C2C12 murine skeletal muscle cells with 20 ng/ml of IGF-I at different time points. The IGF-I treatment increased plectin protein expression in a dose-dependent manner. The mRNA level of plectin was measured by real-time quantitative PCR to determine if plectin induction was regulated pretranslationally. IGF-I treatment resulted in a very rapid induction of plectin mRNA transcript in C2C12 myotubes. Plectin mRNA increased by 140 and 180% after 24 and 48 hours of IGF-I treatment, respectively, and returned to the control level after 72 hours of IGF-I treatment. MACF1 mRNA increased 86 and 90% after 24 and 48 hours of IGF-I treat-ment, respectively, and returned to the control level after 72 hours of IGF-I treatment. These results suggested that the plectin gene is regulated pretranslationally by IGF-I in skeletal muscle cells. In conclusion, IGF-I induces a rapid transcriptional modification of the plectin and MACF1 genes in C2C12 skeletal muscle cells and has modulating effects on a cytolinker protein as well as on contractile proteins.

• BMB Reports
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• v.49 no.1
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• pp.37-44
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• 2016

Spectraplakins are crucially important communicators, linking cytoskeletal components to each other and cellular junctions. Microtubule actin crosslinking factor 1 (MACF1), also known as actin crosslinking family 7 (ACF7), is a member of the spectraplakin family. It is expressed in numerous tissues and cells as one extensively studied spectraplakin. MACF1 has several isoforms with unique structures and well-known function to be able to crosslink F-actin and microtubules. MACF1 is one versatile spectraplakin with various functions in cell processes, embryo development, tissue-specific functions, and human diseases. The importance of MACF1 has become more apparent in recent years. Here, we summarize the current knowledge on the presence and function of MACF1 and provide perspectives on future research of MACF1 based on our studies and others. [BMB Reports 2016; 49(1): 37-44]

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.5
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• pp.75-84
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• 2004

Loess, PAC, MACF and plants were applied to the control of the phytoplankton bloom in laboratory and in field, In field experiment using oil fence, 5ppm concentration of coagulant(PAC) was observed to be effective in controlling the cyanobacterial bloom, resulting in 90% removal of cyanobacteria and phytoplankton from the water column, hi case of Synedra sp., however, only 50% of biomass decreased with the same PAC concentration. MACF(micro-air bubble coagulation and floating), a kind of physicochemical method, was applied to the column of the Kyongan stream and resulted in over 80% chlorophyll a and 73.5% TP removal, Chlorophyll a and total phosphorus were effectively removed from water body when 2.0 g/L of loess with the particle radius of 125 ${\mu}m$ was inputted. In case of experiments involving plants, big cone pine, gingko, and pine needle were observed to be effective in restraining phytoplankton bloom at 0.5g/200ml level. During a field test done at Kyungan stream, where Microcystis heavily occurred, Pine needle and big cone pine were observed to be effective on suppressing algal growth.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.67-67
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• 2023

수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임) 부위별 에탄올 추출물의 항산화 활성에 관하여 총 폴리페놀 함량 측정, 총 플라보노이드 함량 측정, DPPH radical 소거 활성 실험을 실시하였다. 수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임)의 각 부위별 생리활성 실험에서 총 폴리페놀 함량(mg/g)은 청자몽 중피(252.14mg/g)와 청자몽 외피(224.60mg/g), 청자몽 과피(213.13mg/g), 자몽 중피(197.13mg/g) 순으로 모두 150mg/g 이상의 양호한 폴리페놀 함량을 나타내었다. 수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임)의 각 부위별 총 플라보노이드 함량(mg/g)은 자몽 중피(147.24mg/g)와 청자몽 중피(147.08mg/g), 청자몽 과피(127.74mg/g), 청자몽 외피(122.5mg/g) 순으로 모두 120mg/g 이상의 매우 양호한 플라보노이드 함량을 나타내었다. 수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임)의 각 부위별 항산화 활성(EDA, %)은 레몬 과피(83.21%)를 시작으로 라임 전체(80.23%), 레몬 과육(77.02%), 레몬 전체(69.85%), 만다린 과피(68.86%), 라임 과육(64.61%) 순으로 EDA 50% 이상 값을 나타내어 양호한 항산화 활성을 보였다.