• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1303-1307
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• 2007

A tumor suppressor, merlin is a member of ERM family proteins. It consists of N-terminal FERM domain, ${\alpha}-helical$ region, and C-terminal domain. Alternative splicing of merlin's mRNA generates two isotypes of merlin. Isotype I, which has exon17 at the C-terminus instead of exon16 in isotype II, is known to have tumor suppressor activity. Like other ERM proteins, the C-terminal domain of merlin isotype I interacts to its FERM domain. That of isotype II, however, was reported not to bind FERM domain despite the large common part of C-terminal domain, which possibly binds FERM domain. Here, we show the binding of FERM domain to both C-terminal domains of merlin's two isotypes by isothermal titration calorimetry. These results support that merlin isotype II also can form a closed conformation or a multimer by intramolecular or intermolecular interactions using their FERM domain and C-terminal domain.

• International Journal of Naval Architecture and Ocean Engineering
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• v.9 no.3
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• pp.266-281
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• 2017

Hydroelastic interactions of a deformable floating body with random waves are investigated in time domain. Both hydroelastic motion and structural dynamics are solved by expansion of elastic modes and Fourier transform for the random waves. A direct and efficient structural analysis in time domain is developed. In particular, an efficient way of obtaining distributive loads for the hydrodynamic integral terms including convolution integral by using Fubini theory is explained. After confirming correctness of respective loading components, calculations of full distributions of loads in random waves are expedited by reformulating all the body loading terms into distributed forms. The method is validated by extensive convergence tests and comparisons against the counterparts of the frequency-domain analysis. Characteristics of motion/deformation responses and stress resultants are investigated through a parametric study with varying bending rigidity and types of random waves. Relative contributions of componential loads are identified. The consequence of elastic-mode resonance is underscored.

• Honam Mathematical Journal
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• v.22 no.1
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• pp.9-16
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• 2000

An A-module M is catenary if for each pair of prime submodules K and L of M with $K{\subset}L$ all saturated chains of prime submodules of M from K to L have a common finite length. We show that when A is a Noetherian domain, then every finitely generated A-module is catenary if and only if A is a Dedekind domain or a field. Moreover, a torsion-free divisible A-module M is catenary if and only if the vector space M over Q(A) (the field of fractions of A) is finite dimensional.

• Bulletin of the Korean Chemical Society
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• v.30 no.5
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• pp.1039-1042
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• 2009

RNA polymerase II C-terminal domain phosphatase 1 containing ubiquitin like domain (UBLCP1) has been identified as a regulatory molecule of RNA polymerase II. UBLCP1 consists of ubiquitin like domain (UBL) and phosphatase domain homologous with UDP and CTD phosphatase. UBLCP1 was cloned into the E.coli expression vectors, pET32a and pGEX 4T-1 with TEV protease cleavage site and purified using both affinity and gel-filtration chromatography. Domains of UBLCP1 protein were successfully purified as 7 mg/500 mL (UBLCP1, 36.78 KDa), 32 mg/500 mL (UBL, 9 KDa) and 8 mg/500 mL (phosphatase domain, 25 KDa) yielded in LB medium, respectively. Isotope-labeled samples including triple-labeled ($^2H/^{15}N/^{13}C$) UBLCP1 were also prepared for hetero-nuclear NMR experiments. $^{15}N-^{1}H$ 2D-HSQC spectra of UBLCP1 suggest that both UBL and phosphatase domain are properly folded and structurally independent each other. These data will promise us further structural investigation of UBLCP1 by NMR spectroscopy and/or X-ray crystallography.

• The Korean Journal of Ceramics
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• v.6 no.3
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• pp.291-295
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• 2000

The pyroelectric property of $Pb_{0.9}La$_{0.1}TiO_3$ceramics in a range of 1.3-4.1$\mu\textrm{m}$, fabricated by conventional solid sintering, was investigated as a function of poling field. The pyroelectric of the 4.1$\mu\textrm{m}$ of $Pb_{0.9}La$_{0.1}TiO_3$ceramics is higher than that of the 1.3$\mu\textrm{m}$ and 1.7$\mu\textrm{m}$ of $Pb_{0.9}La$_{0.1}TiO_3$ceramics at a low poling field and the pyroelectric coefficient is 25nC/$\textrm{cm}^2$K at a 4kV/mm poling field in every grain size. In order to explain this phenomenon, the intrinsic and extrinsic effects in view of the definition of the pyroelectric coefficient are introduced. The intrinsic and extrinsic effects on the pyroelectric property were investigated by measuring the tetragonal ratio and the $I_{002}$ with temperature with high temperature X-ray diffractometer. The change of spontaneous polarization and the $90^{\circ}$domain wall motion with temperature in the 1.3$\mu\textrm{m}$ and 4.2$\mu\textrm{m}$ of $Pb_{0.9}La$_{0.1}TiO_3$ceramics have no effects on the pyroelectric coefficient. In our study, it can be seen that the pyroelectric coefficient is related to the quantity of $180^{\circ}$domain switching after poling treatment.

• Journal of the Korea Society of Computer and Information
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• v.21 no.9
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• pp.85-90
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• 2016

In this paper, we study the domain-independent sensor data structuring method and the derivations about ' Filter Criteria' expansion factors of heterogeneous domain systems. For this purpose, we propose a 5W1H based information search and retrieval method without knowledges about the target domains. And we also suggest a resource management model that can support expanding search of information. By the suggested method, the oneM2M standard able to implement the use-cases like 'data exchanging between the heterogeneous domains'.

• Bulletin of the Korean Chemical Society
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• v.35 no.9
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• pp.2753-2757
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• 2014

INtegrase Interactor 1 protein (INI1/hSNF5) or BRG1-associated factor 47 (BAF47) is a SWI/SNF-related matrix associated actin dependent regulator of chromatin subfamily B member. DNA binding domain of INI1/hSNF5 is cloned into E.coli expression vectors, pET32a and purified as a monomer using size exclusion chromatography. NMR data show that $INI1^{DBD}$ has folded state with high population of ${\alpha}$-helices. By fluorescence-quenching experiments, binding affinities between $INI1^{DBD}$ and two double stranded DNA fragments were determined as $29.9{\pm}2.6{\mu}M$ (GAL4_1) and $258.7{\pm}5.8$ (GAL4_2) ${\mu}M$, respectively. Our data revealed that DNA binding domain of INI1/hSNF5 binds to transcriptional DNA sequences, and it could play an important role as a transcriptional regulator.

• Communications of the Korean Mathematical Society
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• v.20 no.2
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• pp.339-350
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• 2005

We obtain the following two inequalities on a strongly pseudoconvex domain $\Omega\;in\;\mathbb{C}^n\;:\;for\;f\;{\in}\;O(\Omega)$ $$\int_{0}^{{\delta}0}t^{a{\mid}a{\mid}+b}M_p^a(t, D^{a}f)dt\lesssim\int_{0}^{{\delta}0}t^{b}M_p^a(t,\;f)dt\;\int_{O}^{{\delta}O}t_{b}M_p^a(t,\;f)dt\lesssim\sum_{j=0}^{m}\int_{O}^{{\delta}O}t^{am+b}M_{p}^{a}\(t,\;\aleph^{i}f\)dt$$. In [9], Shi proved these results for the unit ball in $\mathbb{C}^n$. These are generalizations of some classical results of Hardy and Littlewood.

• Journal of the Optical Society of Korea
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• v.9 no.2
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• pp.54-58
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• 2005

A novel mode analysis method and differential mode delay measurement technique for a multimode optical fiber based on optical frequency domain reflectometry has been proposed for the first time. We have used a conventional OFDR with a tunable external cavity laser and a Michelson interferometer. A few-mode optical multimode fiber was prepared to test our proposed measurement technique. The differential mode delay (DMD) of the sample fiber was measured to be 16.58 ps/m with a resolution of 1.5 ps/m. We have also compared the OFDR measurement results with those obtained using a traditional time-domain measurement method.

• Microbiology and Biotechnology Letters
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• v.28 no.4
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• pp.195-201
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• 2000

Glucoamylase of Saccharomyces diastaticus is produced as a large precursor composed of signal peptide (21 amino acid residues), Thr and Ser-rich region and functional glucoamylase. To evaluate the utility of the glucoamylase signal peptide (GSP) for the secretion of foreign proteins, four types of GSP mutants (ml : Pro-18 longrightarrowLeu-18, m2 : Tyr-13 longrightarrowLeu, m3 : Ser-9longrightarrowLeu-9, m4 : Asn-5 longrightarrowPro-5) were constructed and secretion efficiency of each mutant was compared with that of native GSP by the expression and secretion of Bacillus subtilis CMCase under the control of GAP in N-terminal domain and hydrophobic domain. n mutant 4, a polar amino acid was replaced by a helix - breaking Pro residue. CMCase activity assay and Western blot analysis revealed that CMCase secretion by GSP mutants replaced by Leu were increased compared with native GSP. In the case of m2 and m3, the substitution of Leu for Tyr-13 and Ser-9 in the hydrophobic region resulted in a twofold increase in the extracellular CMCase activity.