• Journal of Society of Preventive Korean Medicine
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• v.3 no.1
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• pp.125-145
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• 1999

The effects of Wang-ttum, Magnetic Water, Magnetic field and Sibjeondaebotang on hemodynamics in sleep deprivation were studied. The results as follows; 1. In case of Wang-ttum operated group, significant changes were observed at 12 p.m., 2 a.m., 4 a.m. in maximum blood pressure for the first and second overnight stay and at 2 a.m. for the third and, respectively, average blood pressure at 12 p.m., 2 a.m. for the 1st and 2nd overnight stay, minimum blood pressure at 10 p.m.. 12 p.m.. 2 a.m. for the 1st overnight stay and at 10 p.m., 12 p.m. for the 2nd and at 12 p.m. for the 3rd, pulse rate at 12 p.m., 2 a.m., 4 a.m., 6 a.m., for 1st and 2nd and at 2 a.m., 4 a.m. for the 3rd and 4th, TP-KS at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and 2nd and at 2 a.m., 4 a.m., 6 a.m. for the 3rd, PRP at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and 2nd and at 12 p.m., 2 a.m., 4 a.m. for the 3rd and at 2 a.m., 4 a.m. for the 4th, TPR at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. from 1st to 4th overnight stay. 2. In case of taking magnetic water group, significant changes were observed at 2 a.m., 4 a.m. in pulse rate for the 1st overnight stay and, respectively, PRP at 2 a.m. for the 1st, TRP at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and 4th. 3. In case of attaching magnet group, TPR was significantly observed at 10 p.m. for the 1st overnight stay. 4. In case of medicating Sibjeondaebotang group, significant changes were observed at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. in maximum blood pressure for the 1st and 2nd overnight stay and at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 3rd and at 2 a.m., 4 a.m., 6 a.m. for the 4th and, respectively, average blood pressure at 10 p.m., 12 p.m. for the 1st and 2nd and at 10 p.m. for the 3rd and 4th, minimum blood pressure at 10 p.m., 12 p.m. from 1st to 4th, pulse rate at 2 a.m., 4 a.m., 6 a.m. from 1st to 3rd and at 2 a.m., 4 a.m. for the 4th, TP-KS at 10 p.m., 12 p.m., 2 a.m., 4a.m., 6 a.m. for the 1st and at 10 p.m., 2 a.m., 4 a.m., 6 a.m. for the 2nd and at 2 a.m., 4 a.m., 6 a.m. for the 3rd and at 6 a.m. for the 4th, PRP at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 1st and at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 2nd and 3rd and at 12 p.m., 2 a.m., 4 a.m., 6 a.m. for the 4th, TPR at 10 p.m., 12 p.m., 2 a.m., 4 a.m., 6 a.m. from 1st to 4th. As mentioned obove, the effects of Wangttum and Sibjeondaebotang on hemodynamics in sleep deprivation were observed both the impulse of SIM-YANG and mutual function of QI-HYOL. The effects of Magnetic water and Magnetic field were observed the side of mutual function of QI-HYOL.

• Ecology and Resilient Infrastructure
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• v.2 no.2
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• pp.154-160
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• 2015

This paper presents the field investigation of deposition and erosion relief of a riverfront using vegetation. The results obtained were as follows: Phragmites japonica showed 0.2 m of deposition and 0.3 m-0.4 m of erosion relief of river front by the critical velocity of 1.0 m/s-1.2 m/s. P. communis showed 0.1 m-0.4 m of deposition and 0.2 m-0.3 m of erosion relief by the critical velocity of 0.6 m/s-0.7 m/s. Salix gracilistyla showed 0.1 m-0.2 m of deposition and 0.4 m-0.5 m of erosion relief by the critical velocity of 1.2 m/s-1.4 m/s. Miscanthus sacchariflorus showed 0.1 m-0.4 m of deposition and 0.1 m-0.2 m of erosion relief by the critical velocity of 0.6 m/s-0.7 m/s. S. gracilistyla had the greatest role, while M. sacchariflorus had the lowest role for erosion relief. These results showed that aquatic plants had an effective role in sustaining a stable channel.

• The Journal of the Korea Contents Association
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• v.15 no.6
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• pp.297-302
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• 2015

High-energy medical linear accelerator on the dose to the thyroid cancer during radiotherapy were evaluated using optical stimulation luminescence dosimeters(OSLD) using. Scattered's influence in the case of 3D-CRT 25.4 mSv, 28.8 mSv, 31.3 mSv, 26.5 mSv, 27.4 mSv 5 times with an average 27.9 mSv, in the IMRT 46.8 mSv, 43.2 mSv, 42.3 mSv, 41.5 mSv, 44.1 mSv to five times the average of 43.6 was the result of mSv. In the case of light neutron dosimetry results 3D-CRT 3 mSv, 3 mSv, 3.4 mSv, 3.5 mSv, 3.1 mSv to five times the average 3.2 mSv, in the IMRT 5.1 mSv, 4.8 mSv, 4.2 mSv, 4.8 mSv, 4.9 mSv, to five times the average of 4.7 was the result of mSv. Both parties and the light scattered neutrons were significantly appreciated compared to IMRT 3D-CRT. Treatment of cancer using radiation workers, as in this study, and that a significant amount of scattered rays in the adjacent normal tissues during radiation therapy using energy assessment to influence by fully aware of this information is necessary for the exposure reduction efforts the feed.

• Journal of Acupuncture Research
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• v.22 no.3
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• pp.169-183
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• 2005

Objective : The purpose of this study was to investigate the anti-caner effect of cobrotoxin on the prostatic cancer cell line (PC-3).The goal of study is to ascertain whether cobrotoxin inhibits tile cell growth and cell cycle of PC-3, or the expression of relative genes and whether the regression of PC-3 cell growth is due to cell death or the expression of gene related to apoptosis. Methods : After the treatment of Pc-3 cells with cobrotoxin, we performed 형광현미경, MTT assay, Western blotting, Flow cytometry, PAGE electrophoresis and Surface plasmon resonance analysis to identify the cell viability, cell death, apoptosis, the changes of cell cycle and the related protein, Adk, MAP kinase. Results : 1. Compared with normal cell, the inhibition of cell growth reduced in proportion with the dose of cobrotoxin(0-16nM) in PC-3. 2. Cell viabilities of 0.1, 1, 4nM cobrotoxin treatment were decreased and those of 8, 16nM were decreased significantly. 3. S phase of cell cycle was decreased at the group of 1, 2, 4, 8, 16nM cobrotoxin, but M phase was increased at 0.1, 1, 2, 4, 8, 16nM cobrotoxin. 4. Cox-2 expression after cobrotoxin was peaked at 12hours and was decreased significantly after 6, 12, 24 hours. 5. The expression of Cdk4 was decreased dose-dependently at 1, 2, 4, 8nM cobrotoxin and was decreased siginificantly at 4, 8nM Cyclin D1 was decreased at 1, 2, 4, 8nM and Cycline E was not changed. Cycline B was decreased at 1, 2, 4, 8nM dose-dependently and was decreased siginificanlty at 2, 4, 8nM. 6. The expression of Akt was decreased at 1, 2, 4, 8nM dose-dependently and was decreased significantly at 2, 4, 8nM. 7. ERK was increased at 1, 2nM and decreased at 4, 8nM, p-ERK was increased at 1, 2, 4 nM, but decreased at 8nM. JNK and p-JNK were increased at 1, 4, 8 nM. p38 was increased at 2nM p-p38 was increased at lnM but decreased significantly at 2, 4, 8nM. 8. The nucli of normal cells were stained round and homogenous in DAPI staining, but those of PC-3 were stained condense and splitted. Apoptosis was increased dose-dependently at 2, 4, 8, 16nM and increased significantly at 2, 4, 8, 16nM. 9. Bax wasn`t changed at 1, 2, 4, 8nM and Bcl-2 was decreased significantly at 1, 2, 4, 8nM. Caspase 3 and 9 weren`t changed at 1, 2, 4nM but were decreased significantly at 8nM. Conclusions : These results indicate that cobrotoxin inhibits the growth of prostate Cancer cells, has anti-cancer effects by inducing apoptosis.

• Journal of Plant Biology
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• v.14 no.2
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• pp.1-6
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• 1971

When germination beds of carrot seeds were treated with either 0.01M or 0.05M concentrations of Ca(NO3)2, CaSO4, MgSO4, K2SO4 and KH2PO4, an acceleration in the germination rate was observed in the groups treated with 0.01M KH2PO4 and 0.05M MgSO4 and 0.05M Ca(NO2)3. In earlier work by the author with acetone a similar result was observed and reported. The pH range in these experiments was maintained between 5.0 and 6.0. It was found that the groups treated with 0.05M K2SO4, 0.05M Ca(NO3)2, 0.05M Ca(NO3)2, 0.05M MgSO4, 0.01M KH2PO4, 0.01M Ca(NO3)2 germinated earlier than the control group. The acceleration of the germintion rate varied with the inorganic compounds used in the following descending order; 0.01M KH2PO4, 0.05M Ca(NO3)2, 0.05M K2SO4, 0.05M CaSO4 and 0.05M KH2PO4. As a result of these expriments, it occurs to the author that in the germination of carrot seeds some inorganic compounds appear to activate the osmotic function of carrot seeds causing acceleration in the germination rate.

• Nuclear Engineering and Technology
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• v.16 no.2
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• pp.58-63
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• 1984

Paper electrophoretic separation of octahedrally bonded (Ruc $l_{6}$ )$^{3-}$ has been carried out by using the specially designed migration apparatus. The supporting electrolyte solutions are as follows: 0.1M-HCl $O_4$, 0.05 M-HCl+0.09M-KCl, 0.1M-HCl, 5$\times$10$^{-3}$ M-NTA, 0.01M-HCl, 0.01M-HCl $O_4$, 0.01M-citric acid, 0.01M-K $H_2$P $O_4$+0.01M-N $a_2$HP $O_4$, 0.05M-borax, 0.025M-N $a_2$C $O_3$+0.025M-NaHC $O_3$, 0.01M-N $a_3$P $O_4$, 0.01M-NaOH and 0.1 M-NaOH. The (Ruc $l_{6}$ )$^{3-}$ appears in 2 to 4 peaks and is found in several chemical species such as (RuCl ($H_2O$)$_{5}$ )$^{2+}$, cis and trans (RuC $l_2$($H_2O$)$_4$)$^{1+}$, (RuC $l_3$($H_2O$)$_3$)$^{0}$ , (RuC $l_4$($H_2O$)$_2$)$^{1-}$, (RuC $l_{5}$ ($H_2O$))$^{2-}$ and (RuC $l_{6}$ )$^{3-}$. The retention value has been found to be highest in the 0.025M-N $a_2$C $O_3$+0.025M-NaHC $O_3$ electrolyte solution.n.

• Journal of the Korean Chemical Society
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• v.39 no.6
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• pp.459-465
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• 1995

Acetolactate Synthase (ALS) was partially purified from the yeast and its basic biochemical studies were carried out. Yeast was grown in the minimum media containing 0.5% glucose, 51 mM $K_2HPO_4$, 22 mM $KH_2PO_4$, 8 mM $(NH_4)2SO_4,\;0.4\;m M\;MgSO_4$ for 18 hours at 37 $^{\circ}C$. The cell was ruptured in the buffer (20 mM phosphate buffer pH 7.0, 0.1 mM TPP, 0.5 mM DTT, 1 ${\mu}M$ FAD, and 1 mM MgCl_2$) following an overnight suspension. The supernatant fraction was collected from $10,000{\times}g$ and the enzyme was further purified by ammonium sulfate fractionation, DEAE-Sephacel chromatography and leucine-agarose chromatography. The enzyme activity was measured under the various conditions by the function of protein concentration, time, temperature, pH, and substrate. The optimum temperature was found to be 50$^{\circ}C$, optimum pH 8.0∼8.5. The kinetic parameters, $K_m\;and\;V_{max}$ were 8.4 mM and 17.9 nmol/mg/min respectively. Stability of the enzyme was studied with ethylene glycol and glycerol added to the enzyme solution. Both ethylene glycol and glycerol improved the enzyme stability up to 50%. The study of feedback inhibition showed that valine was a strong inhibitor while leucine was a weak inhibitor.

• Journal of Plant Biotechnology
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• v.4 no.2
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• pp.83-89
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• 2002

To establish in vitro mass production system of grape anthocyanin pigments through callus and cell suspension culture, the effects of nitrogen amount and the ratio of $NO_3^-$/$NH_4^+$ in the medium on cell growth and anthocyanin production were investigated. Total nitrogen amount and the ratio of $NO_3^-$/$NH_4^+$ in the medium strongly affected anthocyanin production and cell growth. When $NH_4^+$ was fixed, the cell growth was promoted by 50 mM total nitrogen (20 mM $NO_3^-$ : 30 mM $NH_4^+$ ) than other nitrogen combinations, and was strongly inhibited when $NO_3^-$ was lacking (0 mM $NO_3^-$ : 60 mM $NH_4^+$ ) while anthocyanin production was increased. When $NO_3^-$ was fixed, the cell growth was promoted by 70 mM total nitrogen (40 mM $NO_3^-$ : 30 mM $NH_4^+$) than other nitrogen combinations, and was strongly inhibited when $NO_3^-$ was lacking (0 mM $NO_3^-$ : 60 mM $NH_4^+$ ) while anthocyanin production was increased. Cell growth was gradually increased by all nitrogen combinations, but anthocyanin production reached its peak on day 4 in culture. Anthocyanin content increased with decreasing cell density. Sucrose was rapidly hydrolyzed to fructose and glucose within 4 days. Glucose and fructose concentrations in the medium increased and peaked at the 4th day. The anthocyanin content of $NH_4^+$-free 2% sucrose media was 2 times (200 $\mu\textrm{g}$/g) higher than that of 1% sucrose. When $NO_3^-$ was lacking, the highest anthocyanin production was observed at 4% sucrose after 12 days of culture, and increased along with the sucrose concentration.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.443-450
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• 2017

The objective of this study was to isolate algae growth inhibiting microorganism to biologically control Microcystis aeruginosa, which is a harmful cyanobacterium. Various bacterial strains were isolated in this study, and four bacterial strains of M1~M4 exhibited remarkable growth inhibiting activity against M. aeruginosa. Based on the 16S rRNA analysis, the isolated M1~M4 strains were identified, and isolated four strains were rod-type and gram-negative. In particular, as well as respective single strain, co-culture of the isolated M1~M4 strains showed obvious algicidal activity against M. aeruginosa. When mixed four strains were inoculated, about 50% of the chlorophyll a was reduced after two days, about 70% after four days, and about 80% after seven days. From these results mentioned above, the four bacterial strains may contribute to the control of harmful M. aeruginosa.

• Journal of Korean Society of Forest Science
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• v.80 no.2
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• pp.193-201
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• 1991

The pollen morphology of 7 taxa, 3 genera of Buxaceae growing in Korea was investigated by means of light(LM) and scanning electron microscope (SEM). The results were as follows ; 1. A key to the genera of Buxaceae on the basis of pollen morphology was provided. 2. Pollen Length that Buxus was $21.9{\mu}m-28.3{\mu}m$, Pachysaradra was $34.9{\mu}m-43.0{\mu}m$ and Sarcococa was $27.2{\mu}m-39.6{\mu}m$ and that muri width and length were $0.3{\mu}m-4.6{\mu}m$, $0.9{\mu}m-4.3{\mu}m$, and that Lumen length was $0.4{\mu}m-2.4{\mu}m$. 3. Sculpture patterns were reticulate (Buxus) and chain shape (Sarcococa and Pachysandra). 4. Aperture type was polyforate.