• Title/Summary/Keyword: M30

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The Study of Pain and Pain Management of Cancer Patients (악성종양 환자의 통증 및 통증관리에 관한 연구)

  • Yoon Gwi-Ok;Park Hung-Sook
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.3 no.2
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    • pp.299-316
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    • 1996
  • This study is the descriptive survey to provide basic data for nursing intervention to pain management of cancer patients by finding more effective way to manage pain with recognize pain level and pain characteristics. To achieve the purpose of this study, the subjects of this study are 110 male or female gastro intestinal tract patients who are older than twenty, are hospitalized in Pusan University Hospital from 1995. 5. 28 to 1995. 9. 25 and have had medical treatment. The modified pain assessment of cancer patients of Cornne, H. Rosermary, M. was used as the tool of study with 16 questionaries. The pain score consists of sensory intensity score and distress score. The data was analyzed by the SPSS statistical program number, percentage, mean, standard deviation, t-test, One Way Anova and Duncan's Multiple Range Test were utilized for analysis. The results were summarized as follows : 1. In population-sociological characteristics : in the age-range of subject, the sixties are most as 32.7% and the subjects after the forties are 89.5%, in sex of subjects, male patients are 66.4% and female 33.6%, in the number of family, the subjects who has 4 or above families are 70% and the subjects who live with their spouse, sons and daughters are 54.5% 2. In the disease characteristics : stomach cancer patients were most as 39.1%. And the most of patient who had never been operated before. In time of pain, the most of subjects were intermittent. In the type of pain, the most of subjects were 'dully pain' as 31.8%. Metastatic subjects were 30.0%. In the origin of pain, nervous pressure was 50.8%. The number of complication was 46 and most of complication are obstruction as 6%. 3. In the pain level, 91subjects complained pain. And mean pain score was $287.1{\pm}116.1$ The mean pain score of female subjects was higher than that of male subjects. 4. In the pain characteristics, the pain began usually at meal time as 40.7%. The duration of pain was mostly from 1 month to 3 months as 57.1%. The appetite was mainly concerned with the pain as 31.8%. The etiology of pain was usually tumor as 69.3%. The meaning of pain was incurable disease as 14.5%, anxiety, death and suffering. 5. The 56(61%) of 91subjects were treated with Analgesic pain management. The kinds of Analgesic is usually valentac as 46.4%. The medication was usually intramuscle as 66.1% at whenever necessary, Response of Analgesic after Medication was usually 'moderate release'. The side effects of medication were nausea as 26.8%. The average amount of morphine dosage hospitalized to cancer patients with pain was 80mg in a day and metastatic cancer patients with pain was 101.9mg in a day. 6. In the relation between the disease characteristics and pain level, there is a significant statistical difference : whether subjects had been operated or not : (t=2.88, p=0.005), time of pain is(t=3.34, p=0.005), stage of metastatic(F=9.323, P=0.0002), and type of pain(F=4.013, p=0.0008). In the pain level of diagnosis, Colon cancer was $353.3{\pm}81.7$(F=2.34, p=0.049), the origin of pain, nerve pressure $316.3{\pm}98.5$(F=2.44, P=0.045), In the complication, ascites and obstruction $324.9{\pm}96.8$(T=2.60, P=0.04).

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Weight Control and Cardiovascular Risk in Middle-Aged Women (중년여성의 체중관리 실태와 성인병 위험도)

  • Kim, Jeong-Ah;Chaung, Seung-Kyo
    • The Korean Journal of Rehabilitation Nursing
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    • v.7 no.1
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    • pp.33-47
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    • 2004
  • The purpose of this study was to investigate weight control practices and cardiovascular risk in middle-aged women. 304 middle-aged women were selected as subjects from thirties to fifthies living at J city in Chung-Buk Do. Data were collected using a questionnaire, anthropometric measurements, BP & total cholesterol level in serum from April 1, to June 30, 2003. The results of this study were as follows: The middle-aged woman's age is average $43.95{\pm}7.09yr$ and mean BMI(body mass index) was $23.54{\pm}3.09\;kg/m^2$. Underweight, normal weight, overweight, obese women were 3.0%, 39.5%, 27.9% and 29.6%respectively. Subjects perceived own as 'Slim' 5.9%, 'ordinary' 45.7%, 'Fat' 48.4%. Their weight perception coincide their own actual body weight but as many as 41.8% of overweight and 9.0% of obese perceived themselves as being 'ordinary'. Middle age women with past weight control experience were 55.6%, and only 35.5% was doing weight control at present. Of these subjects, 71.4% reported wanting to lose weight and the primary reason of weight control was to improve their appearance(39.53%). The most frequently reported weight control behavior was 'exercise' followed 'dieting', but 39.6% reported using 'diet food', 12.4% 'behavior modification', 12.4% 'fasting', and 'diet-drug'(3.6%) or 'smoking'(3.6%). Effective weight control methods were thought regular exercise(97.1%) & dieting (79.3%). And behavior modification(71.4%) or diet camp(60%) were effective, too. An average waist circumference was $79.80{\pm}9.47cm$, waist/hip ratio was $86.63{\pm}6.78$, waist/height ratio was $50.43{\pm}6.10$. In the index of abdominal obesity, 79.7 % of middle-aged women was waist/height ratio over 0.46, 65.3% was waist/hip ratio over 0.85, 28.4% was waist circumference over 85cm. There were significant differences in the degree of abdominal obesity according to age and BMI. In the index of cardiovascualr risk, 10.9% of middle aged women was systolic hypertension over 140mmHg, 18.7% was diastolic hypertension over 90mmHg and 10.6% was hypercholesterolemia over 200mg/dl. There was significant difference in systolic hypertension ratio according to age. There was significant difference in diastolic hypertension ratio according to age and obesity. There were significant differences in hypercholesterolemia accorting to obesity. The abdominal obesity indices and the levels of T-cholesterol in the serum, systolic and diastolic BP increased significantly according to age. T-cholesterol in serum was predicted 2.6% by waist/height ratio. And systolic BP was predicted 15.2% by waist/height, add BMI to 16.8%. Subject's diastolic BP was predicted 12.1% by BMI. Therefore waist/height ratio and BMI were significant factors for the predictors of cardiovascular risk. There was significant correlation between index of obesity and cardiovascular risk. T-cholesterol in serum had correlation with waist/eight ratio(r=0.174) and waist circumference(r=0.48). Systolic BP had correlation with waist/height ratio(r=0.387), and BMI(=0.371). diastolic BP correlation had correlation with BMI(r=0.343) and waist/height ratio(r=0.327). In conclusion, The prevalence of obesity was 29.6% in 304 cases, and increased as age after menopause increased. Middle-aged women's weight perception and actual BMI coincide but some of them did not. Trial to reduce weight was attempted. But most of them did not actually. Undesirable weight control method such as using drugs, fasting, smoking was used by some women. It is important to educate about health weight control methods and raise their awareness of exact body figures. High frequency of abdominal obesity in middle-aged women had correlation with hypertension and hypercholesterolemia. Abdominal index such as waist/height ratio, waist circumference, waist/hip ratio was used. Women's hypertension, hypercholesterolemia significantly related to body mass index and abdominal obesity.

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Assessment of Abnormality in Skeletal Muscle Metabolism in Patients with Chronic Lung Desease by $^{31}P$ Magnetic Resonance Spectroscopy ($^{31}P$ 자기 공명분석법을 이용한 만성 폐질환 환자에서의 골격근대사 이상에 관한 연구)

  • Cho, Won-Kyoung;Kim, Dong-Soon;Lim, Tae-Hwan;Lim, Chae-Man;Lee, Sang-De;Koh, Youn-Suck;Kim, Woo-Sung;Kim, Won-Dong
    • Tuberculosis and Respiratory Diseases
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    • v.44 no.3
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    • pp.583-591
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    • 1997
  • The functional derangement of skeletal muscles which may be attributed to chronic hypoxia has been accepted as a possible mechanism of exercise impairment in patients with chronic obstructive pulmonary disease (COPD). The metabolic changes in skeletal muscle in patients with COPD are characterized by impaired oxidative phosphorylation, early activation of anaerobic glycolysis and excessive lactate and hydrogen ion production with exercise. But the cause of exercise limitation in patients with chronic lung disease without hypoxia has not been known. In order to evaluate the change in the skeletal muscle metabolism as a possible cause of the exercise limitation in chronic lung disease patients without hypoxia, we compared the muscular metabolic data of seven male patients which had been derived from noninvasive $^{31}P$ magnetic resonance spectroscopy(MRS) with those of five age-matched normal male control persons. $^{31}P$ MRS was studied during the sustained isometric contraction of the dominant forearm flexor muscles up to the exhaustion state and the recovery period. Maximal voluntary contraction(MVC) force of the muscle was measured before the isometric exercise, and the 30% of MVC force was constantly loaded to each patient during the isometric exercise. There were no differences of intracellular pH (pHi) and inorganic phosphate/phosphocreatine(Pi/PCr) at baseline, exhaustion state and recovery period between two groups. But pHi during the exercise was lower in patients group than the control group (p < 0.05). Pi/PCr during the exercise did not show significant difference between two groups. These results suggest that the exercise limitation in chronic lung disease patients without hypoxia also could be attributed to the abnormalities in the skeletal muscle metabolism.

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Effect of nitrogen application and clipping height on the vegetative growth of Korean lawn grass (Zoysia japonica Steud.) and Manilagrass (Zoysia mat rella (L.) MERR.) during September/October (질소시용 및 예초고가 한국 잔디(Zoysia japonica Steud.) 및 금잔디(Zoysia matrella MERR.)의 생육후기 영양생장에 미치는 영향)

  • 심재성;윤익석
    • Asian Journal of Turfgrass Science
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    • v.1 no.1
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    • pp.7-17
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    • 1987
  • The experiment with two levels of nitrogen (0. and 300kg / ha / year) and two levels of clipping height (1.5cm and 4cm) was conducted on the field during the period 3 June to 23 October 1985. Clonal lines of korean lawngrass ( Zoysia japonica Steud.) and manilagrass ( Zoysia matrella ( L.) Merr.)of Daejon origin were established in June, as individual clone in rows 30cm apart with a 40cm spacing between clones, actually 4 clones each plot. The results obtained were as follows : 1. When no nitrogen was applied to korean lawngrass, leaf blade which appeared during the August / early September period remained green for a period of about 10 weeks and even leaves emerged in late September lived for 42 days. However, leaf longevity did not exceed 8 weeks as nitrogen was applied. In contrast the leaf longevity of manilagrass which emerged during the mid - August / early September period was 11 weeks and, under the nitrogen applied, 9 weeks, indicating that the life - saen of individual leaf of manilagrass may be longer than that of korean lawngrass. Meanwhile, clipping height had no effect on the leaf longevity in both grasses. 2. During the July / August period, tiller number, green leaf number and DM weight of korean lawngrass were increased significantly with fertilizer nitrogen, but were not with two levels of clipping height. This trend was reversed after late September : no effect of nitrogen was appeared. Instead, lax clipping increased tiller number, green leaf number and DM weight. Green leaves stimulated by lax clipping resulted in the occurrance of more dead leaves in late October. 3. The increase of tiller number, green leaf number, and DM weight of korean lawngrass due to nitrogen application appeared to be of significance in early September. Unlike korean lawngrass, however, this significant increase was maintained to late October when new green leaves still emerge. Clipping height had little effect on the growth of manilagrass by early September, but since then, lax clipping stimulated leaf appearance, possibly resulting in a remained green color of manilagrass turf. 4. Among the stolons outgrown until early September, the primary stolon was not influenced by nitrogen and clipping treatments to produce only 2 - 3 stolons. However, 1st branch stolon as affected by nitrogen increased significantly, so most of stolons which occurred consisted of 1st branch stolon. 5. Until early September, stolon length obtained at nil nitrogen level was chiefly caused by lengthening the primary stolons. By applying nitrogen the primary stolons of korean lawngrass was longer than 1st branch stolons when severe clipping was involved and in turn, shorter than 1st branch stolons when lax clipping was concerned. In manilagrass, 1st branch stolons were much longer than the primary stolons when turf was clipped severely but in conditions of lax clipping, there was little difference in length between primary and 1st branch stolons. 6. Stolon nodes of both korean lawngrass and manilagrass were positively influenced by nitrogen, but no particular increases by imposing clipping height treatment was marked in manilagrass. Although the stolon of korean lawngrass was grown until late october, the growth stimulated by nitrogen was not so remarkable as to exceed that a by nil N. 7. The thickness of korean lawngrass and manilagrass was greatest in late September, but that of manilagrass did not differ significantly from that in late October. 8. The response of stolon length of korean lawngrass to lax clippings was not so great in late October as to that to severe clippings. On the other hand, the positive effect of lax clippings to stolon length in m anilagrass was confirmed even in late October.

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Effects of Heat and pH Treatments on Antioxidant Properties of Ishige okamurai Extract (패 추출물의 항산화능에 미치는 열과 pH의 영향)

  • Kim, Mi-Jung;Choi, Jung-Soo;Song, Eu-Jin;Lee, So-Young;Kim, Koth-Bong-Woo-Ri;Lee, So-Jeong;Kim, Seo-Jin;Yoon, So-Young;Jeon, You-Jin;Ahn, Dong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.50-56
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    • 2009
  • This study was carried out to determine the optimum extraction conditions for Ishige okamurai by comparing the yields, total phenolic compound content (TPC), and antioxidant properties of its 95%, 70%, 50% fermented ethyl alcohol and water extracts. Additionally, the effects of heat and pH treatments on the antioxidant properties of the extracts were evaluated by their TPC and 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging capabilities. The yields of the extracts were greatest in the order of water > 50% > 70% > 95% fermented ethyl alcohol, and the TPC of the 70% (26.18%) and 50% fermented ethyl alcohol (27.56%) extracts were higher than those of the others. However, in terms of DPPH radical scavenging and ferrous-reducing power, the 70% fermented ethyl alcohol extract of Ishige okamurai showed the highest antioxidant effects. Additionally, in the results for the heat and pH treatments, the antioxidant properties of the 70% fermented ethyl alcohol extract were not influenced by the treatment conditions except at pH 10.

A Prediction Model for Forecast of the Onset Date of Changmas (장마 시작일 예측 모델)

  • Lee, Hyoun-Young;Lee, Seung-Ho
    • Journal of the Korean Geographical Society
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    • v.28 no.2
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    • pp.112-122
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    • 1993
  • Since more than 50${\%}$ of annual precipitation in Korea falls during Changma, the rainy season of early summer, and Late Changma, the rainy season of late summer, forcasting the onset days Changmas, and the amount related rainfalls would be necessary not only for agriculture but also for flood-control. In this study the authors attempted to build a prediction model for the forecast of the onset date of Changmas. The onset data of each Changma was derived out of daily rainfall data of 47 stations for 30 years(1961~1990) and weather maps over East Asia. Each station represent any of the 47 districts of local forecast under the Korea Meteorological Administration. The average onset dates of Changma during the period was from 21 through 26 June. The dates show a tendency to be delayed in El Ni${\~{n}}o years while they come earlier than the average in La Nina years. In 1982, the year of El Ni${\~{n}}o, the date was 9 Julu, two weeks late compared with the average. The relation of sea surface temperature(SST) over Pacific and Northern hemispheric 500mb height to the Changma onset dates was analyzed for the prediction model by polynomial regression. The onset date of Changma over Korea was correlated with SST in May(SST${_(5)}{^\circ}$C) of the district (8${^\circ}$~12${^\circ}S, 136${^\circ}~148${^\circ}W)of equatirial middle Pacific and the 500mb height in March (MB${_(3)}$"\;"m)over the district of the notrhern Hudson Bay. The relation between this two elements can be expressed by the regression: Onset=5.888SST${_5}"\;"+"\;"0.047MB${_(3)}$"\;"-251.241. This equation explains 77${\%}$ of variances at the 0.01${\%}$ singificance level. The onset dates of Late Changma come in accordance with the degeneration of the Subtro-pical High over northern Pacific. They were 18 August in average for the period showing positive correlation(r=0.71) with SST in May(SST)${_(i5)}{^\circ}$C) over district of IndiaN Ocean near west coast of Australia (24${^\circ}$~32${^\circ}$S, 104${^\circ}$~112${^\circ}$E), but negativ e with SST in May(SST${_(p5)}{^\circ}$ over district (12${^\circ}$~20${^\circ}$S,"\;"136${^\circ}$~148${^\circ}$W)of equatorial mid Pacific (r=-0.70) and with the 500mb height over district of northwestern Siberia (r=-0.62). The prediction model for Late Changma can be expressed by the regression: Onset=706.314-0.080 MB-3.972SST${_(p5)}+3.896 SST${_(i5)}, which explains 64${\%}$ of variances at the 0.01${\%}$ singificance level.

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The Diagnostic Utility of PET-CT for the Preoperative Evaluation of Lymph Node Metastasis in Gastric Cancer Patients (위암 환자의 수술 전 림프절 전이 평가를 위한 PET-CT의 진단적 유용성)

  • Park, Sung-Hyuk;Cho, Min-Su;Ryu, Hoon;Bae, Keum-Seok;Kim, Ik-Yong;Kim, Dae-Sung
    • Journal of Gastric Cancer
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    • v.8 no.4
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    • pp.250-255
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    • 2008
  • Purpose: The purpose of this study was to assess the diagnostic value of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) for detecting the lymph node (LN) metastasis of gastric cancer. Materials and Methods: 119 patients (M : F=89 : 30; mean age: 64) with gastric cancer were referred for preoperative FDG-PET/CT scanning and spiral enhanced abdominal pelvic CT from June 2006 to July 2008, and these were the subjects of our study. All the patients underwent curative radical gastrectomy and lymph node dissection. A final diagnosis was made for all the patients by the histology of the surgical specimens. Results: Both PET/CT and enhanced CT showed similar sensitivity for detecting regional lymph node metastasis (32.6% vs 39.5%, respectively). PET/CT was more accurate than enhanced CT for detecting regional lymph node metastasis (67.2% vs 63.0%, respectively), and PET/CT showed better specificity (86.8% vs 76.3, respectively) and a better positive predictive value (PPV) (58.3% vs 48.6%, respectively). PET/CT showed better specificity (98.0% vs 88.2%, respectively) and accuracy (79.4% vs 73.9%, respectively) than enhanced CT for detecting early gastric cancer. PET/CT showed better specificity (64.0% vs 52.0%, respectively), a better PPV (60.9% vs 57.1%), a better negative predictive value (NPV) (48.5% vs 46.4%, respectively) and better accuracy (53.6% vs 51.8%, respectively) than enhanced CT for detecting advanced gastric cancer. Conclusion: FDG-PET/CT is more usefulness than enhanced CT for making the preoperative diagnosis of regional LN metastases from gastric cancers.

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PREPARATION OF AMORPHOUS CARBON NITRIDE FILMS AND DLC FILMS BY SHIELDED ARC ION PLATING AND THEIR TRIBOLOGICAL PROPERTIES

  • Takai, Osamu
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2000.11a
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    • pp.3-4
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    • 2000
  • Many researchers are interested in the synthesis and characterization of carbon nitride and diamond-like carbon (DLq because they show excellent mechanical properties such as low friction and high wear resistance and excellent electrical properties such as controllable electical resistivity and good field electron emission. We have deposited amorphous carbon nitride (a-C:N) thin films and DLC thin films by shielded arc ion plating (SAIP) and evaluated the structural and tribological properties. The application of appropriate negative bias on substrates is effective to increase the film hardness and wear resistance. This paper reports on the deposition and tribological OLC films in relation to the substrate bias voltage (Vs). films are compared with those of the OLC films. A high purity sintered graphite target was mounted on a cathode as a carbon source. Nitrogen or argon was introduced into a deposition chamber through each mass flow controller. After the initiation of an arc plasma at 60 A and 1 Pa, the target surface was heated and evaporated by the plasma. Carbon atoms and clusters evaporated from the target were ionized partially and reacted with activated nitrogen species, and a carbon nitride film was deposited onto a Si (100) substrate when we used nitrogen as a reactant gas. The surface of the growing film also reacted with activated nitrogen species. Carbon macropartic1es (0.1 -100 maicro-m) evaporated from the target at the same time were not ionized and did not react fully with nitrogen species. These macroparticles interfered with the formation of the carbon nitride film. Therefore we set a shielding plate made of stainless steel between the target and the substrate to trap the macropartic1es. This shielding method is very effective to prepare smooth a-CN films. We, therefore, call this method "shielded arc ion plating (SAIP)". For the deposition of DLC films we used argon instead of nitrogen. Films of about 150 nm in thickness were deposited onto Si substrates. Their structures, chemical compositions and chemical bonding states were analyzed by using X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy and infrared spectroscopy. Hardness of the films was measured with a nanointender interfaced with an atomic force microscope (AFM). A Berkovich-type diamond tip whose radius was less than 100 nm was used for the measurement. A force-displacement curve of each film was measured at a peak load force of 250 maicro-N. Load, hold and unload times for each indentation were 2.5, 0 and 2.5 s, respectively. Hardness of each film was determined from five force-displacement curves. Wear resistance of the films was analyzed as follows. First, each film surface was scanned with the diamond tip at a constant load force of 20 maicro-N. The tip scanning was repeated 30 times in a 1 urn-square region with 512 lines at a scanning rate of 2 um/ s. After this tip-scanning, the film surface was observed in the AFM mode at a constant force of 5 maicro-N with the same Berkovich-type tip. The hardness of a-CN films was less dependent on Vs. The hardness of the film deposited at Vs=O V in a nitrogen plasma was about 10 GPa and almost similar to that of Si. It slightly increased to 12 - 15 GPa when a bias voltage of -100 - -500 V was applied to the substrate with showing its maximum at Vs=-300 V. The film deposited at Vs=O V was least wear resistant which was consistent with its lowest hardness. The biased films became more wear resistant. Particularly the film deposited at Vs=-300 V showed remarkable wear resistance. Its wear depth was too shallow to be measured with AFM. On the other hand, the DLC film, deposited at Vs=-l00 V in an argon plasma, whose hardness was 35 GPa was obviously worn under the same wear test conditions. The a-C:N films show higher wear resistance than DLC films and are useful for wear resistant coatings on various mechanical and electronic parts.nic parts.

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The Effect of PNF Technique application Using Thera-Band on the Balance and Gait of Females over 65 years old (세라밴드를 이용한 PNF 기법 적용이 65세 이상 여성노인의 균형과 보행에 미치는 영향)

  • Kang, Dal-Won;Kang, Mi-Kyoung;Kang, Eun-Sil;Go, Yu-Ri;Kim, Da-Woon;Kim, Dae-Yong;Kim, Jung-Eun;Kim, Won-Hwang;Kim, Ja-Yeon;Kim, Hwan;Jung, Dae-In;Kim, Myung-Hoon;Kim, Sang-Yup;Lee, Dong-Jin;Kim, Chan-Kyu;Kim, Hyun-Jin
    • Journal of Korean Physical Therapy Science
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    • v.18 no.1
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    • pp.1-10
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    • 2011
  • Purpose: Study on the effect of the use of Proprioceptive Neuromuscular Facilitation(PNF) method by use of the direction and charge regulation which is the advantage of the Thera-band therapy on the walks and balances in old people and comparison with the result after applying the general PNF technique. Method: The study has been performed on 30 females over 65 years old. The study has been done by dividing the object group in 3 patterns, which are number 1, the comparison group of 10, two ones that are applied the PNF technique using Thera-band and third, the ones that are applied only the PNF technique. For the PNF and the Thera-band using PNF, we have divided the group into Combination of Isotonic technique and the Rhythmical stabilization technique according to the patient's acquaintance pattern and applied them to the patient's body. Evaluation was the balancing ability which was calculated by using the BIODEX Balance system / FRT and for the evaluation of walking ability, we have used the speed of walking for 10M / TUG. Result: In the comparison group of 10, the balancing ability and the walking ability did not change much before and after the experiment, which made it possible to compare the group with the other two easily(p>0.05). For the other two groups, we have recognized the enhancement both in the balancing ability and the walking ability, but they did not know much difference between themselves(p<0.05). Conclusion: Though there were not a big difference in the sense of improvement between the Thera-band using PNF and the PNF technique only, we could infer that these two therapy has enhanced much in the walking and balancing ability for people over 65 and through these result we can foresee that not only using the method shown in this study but also by using many advantages of Thera-band, we could diminish the tiredness of healer, enhance the efficiency of exercise in them and also by forming self training program for older people we could help them build the prevention program from falls.

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Inheritance of Kunitz Trypsin Inhibitor and P34 Protein in Soybean Seed (콩 종자에서 쿠니츠트립신인히비터와 P34 단백질의 유전)

  • Han, Eun-Hui;Sung, Mi-Kyung;Baek, Woon-Jang;Shim, Sang-In;Kim, Min-Chul;Chung, Jong-Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.1
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    • pp.78-82
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    • 2012
  • Soybean [$Glycine$ $max$ (L.) Merr.] protein is a high quality source for food and feed. But, antinutritional factors in the raw mature soybean are exist. Kunitz trypsin inhibitor (KTI) protein is a main antinutritional factor in soybean seed. Also, P34 protein, referred as $Gly$ $m$ Bd 30K, has been identified as a predominant immunodominant allergen. Genetic relationship between KTI protein and P34 protein could be useful in soybean breeding program for the genetic elimination or reduction of these factors. The objective of this study was to determine the independent inheritance or linkage between KTI protein and P34 protein in soybean seed. A total of 479 $F_2$ seeds were obtained from the cross of 07B1 and PI567476 parents. KTI protein and relative amount of P34 protein were analysed from $F_2$ seeds harvested from the F1 plants by using SDS-PAGE and Western blot analysis. The segregation ratios of 3 : 1 for KTI protein (353 KTI protein present : 126 KTI protein absent) and relative amount of P34 protein (363 normal amount of P34 protein : 116 low amount of P34 protein). The segregation ratio of 3 : 1 suggested that KTI protein and relative amount of P34 protein in mature soybean seed were controlled by a single major gene. The segregation ratios of 9 : 3 : 3 : 1 (266 KTI protein present, normal amount of P34 protein: 88 KTI protein present, low amount of P34 protein: 102 KTI protein absent, normal amount of P34 protein: 23 KTI protein absent, low amount of P34 protein) and Chi-square value (${\chi}^2$=3.31, P=0.346) were observed in $F_2$ seeds. This data showed that KTI protein was inherited independently with relative amount of P34 protein in soybean. These results will be helpful in breeding program for selecting the line with lacking KTI protein and reduced amount of P34 protein in soybean.