• Korean Journal of Animal Reproduction
• /
• v.18 no.4
• /
• pp.265-274
• /
• 1995

가토 20마리의 혈액중 혈소판의 수를 수정 전후로 조사한 결과, 수정전과 비교하여 수정후 1일부터 유의하게 감소하였으며 (26.84%, P<0.01), 이러한 감소는 수정후 5일까지 계속되었다. 수정후 5일동안 혈소판의 감소율과 혈소판의 최저치를 수정란의 수와 비교한 바, 이들간의 유의한 상관관계(r=0.5032)가 있었다. 가토수정란 (2세포기) 각각 30∼40개를 5마리의 비장적출수란가토의 난관에 이식한 다음, 혈소판수의 변화를 조사한 결과, 이식전에 비해 이식후 90분 (P<0.025)과 135∼270분 (P<0.025)에 유의한 감소를 확인하였따. 특히 수정란 이식후 180분경에 가장 크게 감소하였다 (P<0.001). 60∼70개의 2세포기 가토수정란을 24시간 배양한 다음, 배양액을 3마리의 비장적출가토에 주사한 후 혈소판의 변화를 조사하였을 때, 주사후 120분경부터 혈소판의 수가 유의하게 감소하기 시작하였다. 이들 배양액을 동결, 해동한 다음 주사한 실험에서도 유사한 결가를 얻었다. 또한 이 배양액을 비장적출생쥐에 주사한 경우에도 가토에서와 마찬가지의 혈소판 촉진현상이 나타났다. 혈소판 촉진인자(PAF)의 길항제인 kadsurenone이 첨가된 배양액에서 24시간동안 가토수정란을 배양한 다음, 이 배양액을 4마리의 비장적출생쥐에 주사한 결과, 혈소판수의 변화가 일어나지 않았다. 또한 kadsurenone이 첨가된 배양액에서는 2세포기 가토수정란의 상실기와 배반포기까지 발달율은 각각 4와 7%로 대조구의 7과 49%보다 유의하게 낮았다. 따라서 본 연구에서 가토의 초기배는 가토나 생쥐의 혈소판수를 감소시키고, 특히 길항제 처리는 이러한 혈소판 감소현상을 억제시킬 뿐만 아니라 가토 초기배발달을 억제한다는 것을 확인하였다. 결론적으로 가토초기배에서는 PAF 또는 수정란 유래의 PAF가 분비된다는 것을 알 수 있었으며, 이러한 인자는 동결처리에서도 그 기능은 전혀 변하지 않는다고 본다. 이후에 있어서 mouse LIF의 첨가는 돼지의 수정란을 배반포 이후의 단계에까지 발달시킬 수 있었다. 있어서 더 적합한 것으로 판단되었다. 5. 개발된 모형은 논 관개의 물리적 측면과 관리목표 모두를 고려한 것으로 계산된 효율은 벼, 생육 각 단계에서의 효율 비교에 양호한 방법임을 알 수 있다.은 Sharpsburg 점질양토에 대한 S.C.S 한계허용치 10ton/ha/year 이내로 나타났다. 비처리구에서의 토양유실량은 평균 2.56ton/ha/year로 높게 나타난 반면 3개의 서로 다른 추리구인 비수구, 초생수로구 및 Bromegrass구에서는 각각 0.152, 0.192 및 0.290ton/ha/year로 낮은 결과를 가져왔다. 6. 평균 침전량에 대한 L.S.D. 검정 걸과 전시험구중 비처리구가 고도의 유의차를 나타낸 반면 비수구, 초생수로구 및 Bromegrass 목초구 간에는 아무런 유의차가 인정되지 않았다. 7. 농지보전 처리구인 배수구와 초생수로구는 비처리구에 비해 낮은 침두 유출량과 낮은 토양유실량을 나타내었다.구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가 있음을 시사한다.se that were all low in two aspects, named "the Nonsignificant group". And the issues were high risk perception in general

• Journal of Radiation Protection and Research
• /
• v.47 no.4
• /
• pp.204-213
• /
• 2022

Background: The accident at the Fukushima Daiichi nuclear power plant increased the level of anxiety related to the radioactive contamination of various foods sourced in Japan. Particularly, after the accident, the detection of artificial radionuclides in locally produced foods raised food safety concerns. In this study, the radioactivity concentrations and annual ingestions of ⁴⁰K and ¹³⁷Cs in food products commonly and frequently consumed by the general public were investigated, and the annual effective dose of each was evaluated. Materials and Methods: The 2016-2018 data from the Radiation Safety Management Report released by the Korea Nuclear Safety Technology Center was referenced for the evaluation of the amounts of ⁴⁰K and ¹³⁷Cs contained in food. Using the food-ingestion survey mentioned above as a reference, we selected 62 foods to include in our radioactivity concentration and dose assessment. We also developed a questionnaire and evaluated the responses from the subjects who answered the questionnaire. Results and Discussion: The radioactivity concentration of ¹³⁷Cs was found to be close to or below the level of minimum detectable activity. Additionally, the annual ingestion of 62 foods was 294.77 kg/yr, the effective doses from ⁴⁰K and ¹³⁷Cs were 136.4 and 0.163 μSv/yr, respectively. Conclusion: Thus, the findings confirmed that the effective dose from ⁴⁰K and ¹³⁷Cs in food tends to be lower than the effective dose limit of 1 mSv/yr suggested by the International Commission on Radiological Protection (ICRP) Publication 60. The questionnaire developed in this study is expected to be useful for estimating the annual effective dose status of Korean adults who consume foods containing ⁴⁰K and ¹³⁷Cs.

• Analytical Science and Technology
• /
• v.21 no.6
• /
• pp.510-517
• /
• 2008

This study has been described the metabolism and excretion in a healthy male urine collected for 26hrs after oral administration of diclofenac. To detect conjugated metabolites of diclofenac, urine sample was acid-hydrolyzed under the conditions of 6M-HCl at over $110^{\circ}C$ for 1hr. During the acidic hydrolysis process, diclofenac and its metabolites were converted into their corresponding lactam-ring through dehydration reaction. As results of chemical conversion by means of hydrolysis, the structures of diclofenac and its metabolites were also changed acidic to basic forms. However, lactam-ring was degraded by hydroxyl ion at basic condition. Thus, the extraction rate of dehydrated diclofenac and its metabolites was not favored at basic condition. For the determination of trace amounts of diclofenac and its metabolites in urine, trimethylsilylation (TMS) with MSTFA was applied and followed by analysis with gas chromatograph-mass spectrometer. In this study, four metabolites that are formed by the hydroxylation of parent drug were mainly detected. Each metabolite was tentatively identified by both interpretation of mass spectra and comparison with previously reported results. In addition, time profile of urinary excretion rate for parent drugs and metabolites was studied. Finally, the metabolic pathway of diclofenac was suggested on the basis of the elucidation of its metabolites and excretion profiles.

• Journal of the Korean Society of Food Culture
• /
• v.22 no.3
• /
• pp.342-352
• /
• 2007

We investigated the intake trends of street foods for middle school, high school and colleges students through the survey study. In addition, the factors affecting the students' consumption of street foods and their perception toward hygiene status of street vendor were studied. The levels of total aerobic bacteria, coliform groups and enterobacteriaceae in Kimbab, eomuk, and eomuk-kukmul were counted using 3M petrifilms. More than ninety seven % of the respondents had experienced street foods. All the respondents ate street foods 1 to 5 times a month. Forty percent of the respondent stated that 'good taste' and 'time-saving convenience' were their reason for eating the street foods. Their favorite foods were ddokbokgi and various fried foods. All the students responded that frying oil, drinking water, and soy sauce as well as the street vendors' attire and their cooking tools in street vendor were unsanitary. Forty percent of the respondents ranked frying oil as the most unsanitary substance. Eighty percent of the respondents responded that an enforcement of hygiene should be imposed on the street foods vendor. There were significant differences (p<0.001) among the student's age in the opinion related to the reasons of eating street foods, hygiene enforcement, existence of street foods and interest of hygiene status of street foods. High correlation (p<0.05) between the frequency of street foods and snack intakes was shown, regardless of student's age. The result shows that those who eat snacks more than once a day tend to eat street foods more frequently. Aerobic plate counts in Kimbab in 5 different vendors exceeded 10$^5$/g and contamination levels of enterobacteriaceae or coliform in Kimbab exceeded more than 10$^3$/g, which did not satisfy the microbiological standards. In conclusion, although the respondents thought that the sanitation of street foods was poor, most of them want the street food vendor to be maintained with better hygiene condition. This indicated that the regulation for safe street foods should be enforced and educational information about the preparation and serving for safe street foods should be provided to street food vendor.

• Korean Journal of Pharmacognosy
• /
• v.54 no.2
• /
• pp.72-79
• /
• 2023

MRSA is Staphylococcus aureus resistant to β-lactam antibiotics, and is a worldwide infectious disease. Even with the discovery of new antibiotics, resistance develops rapidly, so new alternatives are needed. Jakyakgamcho-tang (JGT) is a combination of Jakyak and Gamcho, and has been mainly used as an antispasmodic and analgesic in oriental medicine. This study was conducted to find out whether there is an effect on MRSA in relation to the anti-inflammatory effect of JGT and the antibacterial effect of Jakyak and Gamcho found in previous studies. In this study, in order to investigate the antibacterial activity of JGT and the combined effect of existing antibiotics, after extracting JGT with 70% EtoH, the disc diffusion method, minimum inhibitory concentration (MIC), drug combination effect (FICI), and time-kill analysis (Time-kill assay), metabolic inhibition, Western blot and qRT-PCR analysis were used to confirm the antibacterial activity mechanism of MRSA of JGT. As a result of the experiment, all of MRSA showed antibacterial activity in JGT's disc diffusion method, and the MIC was 250-1000 ㎍/mL. When existing antibiotics and JGT were combined with drugs, most had synergy or partial synergy. In addition, it was confirmed that the degree of bacterial growth was suppressed over time when simultaneous administration for 24 hours. JGT showed a synergistic effect when administered together with the ATPase-inhibitor DCCD, suggesting that it affected the inhibition of ATPase. As a result of observing the expression of PBP2a, and hla protein in the JGT-treated group and the untreated control group through wstern blot, it was confirmed that the protein expression of the JGT-treated group was significantly suppressed, and the expression levels of mecA, mecR1 and hla genes were also suppressed during JGT treatment. was observed by qRT-PCR. Combining the results of the experiment, it can be seen that JGT has antibacterial activity in MRSA, and when combined with existing antibiotics, the effect was increased compared to treatment with the drug alone. This suggests that JGT can be an alternative to treatment for antibiotic resistance of MRSA.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2022.05a
• /
• pp.67-67
• /
• 2022

국가 물관리 측면에서 증발산량과 토양수분량은 자연계 손실로서 국내 수자원 총량의 약43%(563억 m³/년)를 차지하며, 수자원의 계획과 개발, 물순환 과정 규명 및 다양한 수재해 분석 등을 위한 수문 요소이다. 정부는 2005년 「수문조사 선진화 5개년 계획」과 2008년 「제1차 수문조사기본계획(2010~2019년)」을 통해 2019년까지 증발산량과 토양수분량 관측소 확대(각각 25개 지점) 기반을 마련하였고 「수자원의 조사·계획 및 관리에 관한 법률」에 따라 매년 공인 수문 자료로 증발산량과 토양수분량을 측정하고 있다. 증발산량과 토양수분량은 댐 유역의 정밀한 물순환 해석에도 매우 중요한 정보로서 현재 K-water에서의 관측은 일부 시험유역(용담댐 유역)의 flux tower에 의한 에디공분산법(Eddy Covariance Method) 및 토양수분 센서(TDR, Time Domain Reflectometery)에 의한 지점 자료의 생산만 각각 이루어지고 있다. 본 연구에서는 K-water 댐 유역의 증발산량 및 토양수분량 관측 현황과 그간 관측된 자료의 특성을 각종 경향성 분석 등과 함께 소개하고자 한다, 증발산량의 경우는 2개소의 flux tower를운영(덕유산 지점 2011년 이후, 용담 지점 2017년 이후)하고 있으며, 토양수분량은 총 7개소(계북, 천천, 상전, 안천, 부귀, 주천 지점 2013년 이후, 장계 지점 2017년 이후)에 TDR센서를 설치, 계측 운영 중이다. 이렇게 관측된 자료는 매년 홍수통제소 주관 관련 전문가 공인심사를 통해 일자료 기준으로 한국수문조사연보에 수록되고 있으며, K-water에서도 연보를 통해 공개된 자료를 기준으로 공공데이터포털(data.go.kr) 등과 연계하여 온라인 자료 서비스 중이다. 한편, 최근 2020년 「제2차 수문조사 기본계획(2020~2029년)」에서는 수자원 위성 개발연구와 연계하여 위성을 활용한 증발산량과 토양수분량 산정 연구의 필요성이 강조되고 있다. 하지만 본 연구에서 살펴본 지점 자료만으로는 댐 유역을 포함한 광역단위의 시계열 공간정보를 생산하기 한계가 있으며, 댐 유역과 국내 전 지역의 공간 시계열 증발산량 및 토양수분량 자료 산정과 활용 방안에 대해 정립하고, 나아가 위성영상을 활용한 댐 유역 증발산량·토양수분량 관측 가이드라인 마련 등을 위해서는 국가적으로 많은 재원의 투입과 노력이 필요한 상황이다.

• Journal of the Korean Burn Society
• /
• v.21 no.1
• /
• pp.17-21
• /
• 2018

Purpose: This study aimed to evaluate the thermal protective function of firefighter clothes and gloves through real scale fire simulations. Methods: Firstly, the fire simulation by real scale flame was performed for firefighter clothes. A manikin equipped with firefighter clothes was directly exposed to flames which energy average is 84 Kw/m². for 22 seconds. Heat flux gauges attached on the body measured surface temperature elevation. Secondly, we also performed the other fire simulation by hot plate exposure to firefighter gloves. Firefighter gloves with heat flux gauges exposed hot plate which temperature is 300℃ in both dry and moist conditions. Primary outcome was surface temperature change of manikin body (first simulation) and hand (second simulation) over times. Results: In the first flame simulation, the surface temperature of face and shoulders elevated more rapidly comparing with the other body surface area when initial period of flame shutter open. After 18sec of shutter open, the surface temperature of upper trunk elevated rapildy. After shutter closure, high surface temperature kept continuously on right side of face and left shoulder. In the second hot plate simulation, fingers and palms showed higher surface temperature than the other areas of hands in the both dry and wet conditions. Conclusion: This study suggests that the real scale flame enables firefighter clothes to lose their heat protective function suddenly after 18 seconds. Additionally, the protective function of firefighter gloves were relatively weaker in the palmar side of fingers than the other parts of hand. There should be additional study for evaluate thermal protection performance of firefighter clothes. And, further effort for reinforce palmar side of fingers of firefighter gloves should be done.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2022.05a
• /
• pp.234-234
• /
• 2022

도로에서의 우수를 원활하게 처리하기 위해서 빗물받이 및 연결관 등의 노면 배수시설이 설치되고 있으며, 노면 배수는 측구부를 통해 흘러 빗물받이 유입부로 차집되고 연결관을 통해 하수관거로 배수된다. 그러나 최근 국내 기상패턴의 변화로 국지성 집중호우와 같이 시간당 강우량 증가로 도로부와 저지대에서 배수시설의 배수불량에 따른 도심지 내수침수 피해가 발생하고 있다. 이에 정부에서는 다양한 우수관거 개선사업, 빗물펌프장, 지하저류조와 같은 방재시설을 설치하고 있으나 우수유출저감시설은 대규모 예산이 소요되고 실제 침수지역에 피해 저감효과에 대한 효용성 문제에 대한 제기뿐만 아니라 과밀화된 도심지에서는 지하공간 활용에 한계가 있는 실정이므로 도심지의 다양한 공간을 활용한 도시 배수 및 저류시설에 대한 연구가 필요하다. 따라서 본 연구에서는 유휴 공간인 도로 측구부 공간을 활용하여 도로 노면수를 저류 및 지체할 수 있는 노면수 측구 저류시설의 개념을 제시하고 측구저류조의 활용성을 판단하기 위하여 빗물받이 유입구, 빗물받이, 측구 저류조 및 빗물받이와 측구저류조 연결부에서의 노면수 유입, 유출 및 저류 등의 다양한 흐름 변화를 확인하기 위하여 Fluent 모형의 적용성을 분석하였다. 수치모의 전체 형상은 50x50cm 크기의 빗물받이를 기준으로 양쪽에 2m 길이의 측구 저류조를 원형관으로 연결하여 1/5 축소모형으로 구성하고 격자는 빗물받이 유입부, 빗물받이 및 측구 저류조 내부의 복잡한 3차원 흐름을 모의하기 위해 사면체와 육면체로 조밀하게 생성하였다. 다상유동해석을 위해 VOF(Volume of Fluid)방법을 적용하였고, 수치해석 방법으로는 비정상류, 난류 모형으로는 SST k-ω모형을 적용하였다. 수치모의 조건으로는 설계빈도별(5~30년) 우수유출량을 산정하여 유입 유량별 기존 빗물받이 유입부에서의 유입흐름, 빗물받이 내부에서의 와 발생흐름, 측구 저류조 및 연결관에서의 흐름을 구현하여 분석하였다. 수치모의 결과 빗물받이 유입부에서 연결관을 통한 측구 저류조로 유입되는 유입흐름과 빗물받이 하단부의 배수관을 통해 유출되는 흐름을 정상적으로 구현하였으며, 빗물받이 유입부 및 측구 저류조 연결관에서의 유속변화도 확인할 수 있었다. 또한 빗물받이와 측구 저류조에서 다양한 흐름을 구현하기 위한 Flunet 모형의 적용성을 검토하였으며, 향후 수리실험을 통하여 실제 흐름과의 매개변수 최적화 및 다양한 도로 조건의 변화를 고려한 수치모의 분석을 통하여 지속적인 모형의 검증이 가능할 것으로 판단된다.

• Exercise Science
• /
• v.21 no.3
• /
• pp.289-298
• /
• 2012

In the present study, we investigated the effect of treadmill exercise on apoptotic neuronal cell death in the retinas of streptozotocin-induced diabetic rats. Twenty-eight male Sprague-Dawley rats were used for this study. The animals were divided into four groups(n = 7 in each group):(1) control group, (2) exercise group, (3) diabetes-induced group, (4) diabetes-induced and exercise group. Diabetes mellitus(DM) was induced by intraperitoneal injection of streptozotocin. The rats in the exercise groups were forced to run on the treadmill for 30 minutes once a day, five times per a week, during 12 weeks. In this study, a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay and western blot for the expressions of caspase-3, cytochrome c, Bax, and Bcl-2 in the retinas were conducted for the detection of apoptotic retinal cell death. The present results showed that the number of TUNEL-positive cells was increased in the retinas of the diabetic rats, whereas treadmill exercise suppressed this number. The expressions of pro-apoptotic factors caspase-3, cytochrome c, and Bax were enhanced and the expressions of anti-apoptotic factor Bcl-2 was decreased in the retinas of the diabetic rats. In contrast, treadmill exercise suppressed the expressions of caspase-3, cytochrome c, and Bax and increased the expression of Bcl-2. The present study demonstrated that treadmill exercise suppressed diabetes-induced apoptotic neuronal cell death in the retinas. Based on the present results, treadmill exercise may be effective therapeutic strategy for the alleviating complications of diabetes patients.

• Journal of Korea Entertainment Industry Association
• /
• v.13 no.8
• /
• pp.435-446
• /
• 2019

The purpose of this study was to investigate the effect of strength exercise program(60 minutes/day, 3 times/week) on health-related fitness and the factor of metabolic syndrome in the older at senior care facilities during 12 weeks. Twenty-seven volunteers were recruited for the study and divided into two groups: 15 persons(82.6yrs) undertook a continuous 12 weeks strength exercise program(EG), and 12 persons(78.9yrs) served as a control group(CG). The data process of this study calculated as mean(M) and standard Deviation(SD) of all measured value, used repeated measure ANOVA, and paired t-test. The significant level of hypothesis verification is set-up as α=.05. After 12 weeks in EG, health-related fitness increased by an average of 15.2% for right grip strength(p<.05), 26.9% for left grip strength(p<.05), 32.7% for chair stand(p<.05), 92.5% for one leg stand(p<.05), 29.6% for sit and reach(p<.05), and 34.5% for TUG(p<.05); CG showed no difference between pre and post. In the factor of metabolic syndrome, Systolic BP(3.1mmHg) and fasting blood glucose(7.4mg/dl) decreased in the EG group(p<.05). In summary, the strength exercise program leads to a genuine increase in health-related fitness and decrease in the factor of metabolic syndrome in the older adults. The well planned strength exercise seems to be an important intervention to improve function fitness in this population.