• Molecules and Cells
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• v.43 no.8
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• pp.705-717
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• 2020

While the growth factors like insulin initiate a signaling cascade to induce conformational changes in the mechanistic target of rapamycin complex 1 (mTORC1), amino acids cause the complex to localize to the site of activation, the lysosome. The precise mechanism of how mTORC1 moves in and out of the lysosome is yet to be elucidated in detail. Here we report that microtubules and the motor protein KIF11 are required for the proper dissociation of mTORC1 from the lysosome upon amino acid scarcity. When microtubules are disrupted or KIF11 is knocked down, we observe that mTORC1 localizes to the lysosome even in the amino acid-starved situation where it should be dispersed in the cytosol, causing an elevated mTORC1 activity. Moreover, in the mechanistic perspective, we discover that mTORC1 interacts with KIF11 on the motor domain of KIF11, enabling the complex to move out of the lysosome along microtubules. Our results suggest not only a novel way of the regulation regarding amino acid availability for mTORC1, but also a new role of KIF11 and microtubules in mTOR signaling.

• Journal of the Chungcheong Mathematical Society
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• v.24 no.1
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• pp.11-17
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• 2011

We introduce the notion of weak M-precontinuity which is a generalization of M-precontinuity defined between spaces with minimal structures. We also investigate some properties and characterizations for the continuity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.3
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• pp.153-160
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• 2002

To investigate the effects of the exogenous N $O_3$$^{[-10]}$ supply level on the nitrate assimilation and growth during the vegetative growth stage, and on the accumulation of organic reserves during the successive regrowth period, dry matter (DM), the amount of nitrogenous compounds, total N and starch in alfalfa plants grown hydroponically with 0.2, 1.0 and 3.0 mM KN $O_3$ was estimated, respectively, during vegetative growth period and two cycle of regrowth. When compared with DMs and N contents in various N compounds in the organs grown with 1.0 mM N $O_3$$^{[-10]}$ , N starvation symptoms were found in 0.2 mM and a depressive effect was observed in 3.0 mM after 10 weeks of vegetative growth. Total starch content in root system gown with 0.2, 1.0 and 3.0 mM N $O_3$$^{[-10]}$ during the first regrowth was 50.96, 15.47 and 6.37 mg plant$^{-1}$, respectively. Starch was contained mainly in taproots. The starch content was not significantly changed by 24 days of the second regrowth with 1.0 mM N $H_4$N0$_3$. Total nitrogen content in root system grown with 0.2, 1.0 and 3.0 mM N $O_3$$^{[-10]}$ during the first regrowth was 6.66, 8.43 and 11.09 mg plant$^{-1}$ , respectively. Nitrogen was contained mainly in lateral roots; 80% (in 0.2 mM), 74% (1.0 mM) and 76% (3.0 mM) of total nitrogen in root system. Total N content in root system at the end of the second regrowth also closely affected by the N $O_3$$^{[-10]}$ supply level during the first regrowth. These results suggest that the level of N $O_3$$^{[-10]}$ may strongly influence the accumulation of organic reserves in root system, and that the initial level of organic reserves for the successive regrowth was one of the determinants for shoot regrowth.

• Proceedings of the Korean Statistical Society Conference
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• 2002.11a
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• pp.273-277
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• 2002

In this paper, with martingale argument we derive the explicit formula for the Laplace transform of the busy period of M/M/1 queue with bounded workload which is also called finite dam. Much simpler derivation than appeared in former literature provided.

• Journal of the Speleological Society of Korea
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• v.19 no.20
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• pp.107-112
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• 1989

본지역의 전북 북동군 산간지역에 해당되는 군으로 주변이 덕유산(1,594m)을 중심으로 적상산(1,034m), 두문산(1,052m), 봉선산(884m), 국산봉(756m), 등 1,000내외의 산지로 둘러싸여 있고, 그 사이를 남대천이 흘러가면서 개석하여 심산유곡을 만든 곳이다.(중략)

• The Korean Journal of Food And Nutrition
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• v.12 no.1
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• pp.43-49
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• 1999

The effects of purine catabolites in growth media on the Serratia marcescens purine nucleoside phos-phorylase activity were examined. The enzyme activity was decreased above 60% by guanosine(5 to 15mM). The enzyme activity was not affected at low concentration of inosine (0.1∼1mM). The en-zyme activity was decreased approximately by 40∼50% in the presence of high concentrations of aden-osine hypoxanthine and xanthine (5∼15mM) but was not affected at low concentration of adenosine hypoxanthine and xanthine (0.1∼0.5mM). However the enzyme activity was increase by 20% with low concentrations of uric acid(0.5mN). but was decreased by 80% with high concentrations of same purine catabolite (15mM). Also the enxzyme activity was increased by 20% with low concentrations of glyoxylate (0.5mM) final degradative product of uric acid but was decreased by 30∼50% with high con-centrations of glyoxylate (3∼15mM). The enzyme activity was decreased approximately by 20% by the simultaneous addition of inosine hypoxanthine and uricacid at 5mM each whereas it was increased by 22 and 33% by the combination of inosine and uric acid three purine catabolites at 0.5mM respectively These data suggest that S. marcescens purine nucleoside phosphorylase is positively regulated by a glyox-ylate concentration and then may play a regulatory role in a purine catabolism.

• Journal of applied mathematics & informatics
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• v.23 no.1_2
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• pp.471-477
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• 2007

In this paper we establish some recurrence relations satisfied by the quotient moments of the upper record values from the Weibull distribution. Suppose $X{\in}WEI({\lambda})\;then\;E(\frac {X^\tau_U(m)} {X^{s+1}_{U(n)}})=\frac{1}{(s-\lambda+1)}E(\frac {X^\tau_U(m)}{X^{s-\lambda+1}_{U(n-1)}})-\frac{1}{(s-\lambda+1)}+E(\frac{X^\tau_U(m)}{X^{s-\lambda+1}_{U(n)}})\;and\;E(\frac {X^{\tau+1}_{U(m)}}{X^s_{U(n)}})=\frac{1}{(r+\lambda+1)}E(\frac{X^{\tau+\lambda+1}_{U(m)}}{X^s_{U(n-1)}})-\frac{1}{(\tau+\lambda+1)}E(\frac{X^{\tau+\lambda+1}_{U(m-1)}}{X^s_{U(n-1)}})$.

• Toxicological Research
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• v.14 no.4
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• pp.587-594
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• 1998

In order to understand the mechanism if the regulation of CYP 1A1 gene expression and ethoxyresorufin deethylase (EROD) activity in ex vivo system, we have studied the action of TCDD and 3MC in the isolated perfused female rat liver. CYP1A1 mRNA level and EROD activity were measured in rat liver that was isolated and perfused with various chemicals such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3MC), 17$\beta$-estradiol (E$_2$), morin. TCDD or 3MC alone perfusion into female rat liver resulted in increase of CYP 1A1 mRNA level and the magnitude of stimulation was six times higher with TCDD treatment than 3MC treatment. However E$_2$ perfusion into female rat liver showed inhibition of CYP 1A1 mRNA level. When 10$^{-8}$ M E$_2$ was administered concomitantly with either 10$^{-9}$ M TCDD or 10$^{-9}$ M 3MC, stimulated CYP 1A1 mRNA by either TCDD or 3MC was inhibited. Morin was examined for its effects on CYP 1A1 mRNA level and result was similar to that was observed with estrogen. EROD activity was also stimulated with either TCDD or 3MC perfusion, and the magnitude of EROD stiumlation was smaller than that of CYP 1A1 mRNA stimulation in response to TCDD or 3MC perfusion. Unlike CYP1A1 mRNA level, stimulation of EROD activity was greater with 3MC than TCDD. Concomitant perfusion either E$_2$ or morin with TCDD or 3MC inhibited 3MC perfusion or TCDD perfusion stimulated EROD activity. These data suggested that TCDD and 3MC might act diffrently in terms of regulation of CYP 1A1 gene expression in rat liver.

• Journal of Life Science
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• v.28 no.2
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• pp.148-152
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• 2018

The patchiness of local environments within a habitat is assumed to be a primary factor affecting the spatial patterns of plants, and a randomization procedure is developed for testing the null hypothesis that only spatial association with patches determined the spatial patterns of plants. Chloranthus japonicus (Chloranthaceae) is an herbaceous perennial and a member of the genus Chloranthus in the family Chloranthaceae. The spatial pattern of C. japonicus was analyzed according to several patchiness indices, population uniformity or aggregation under different sizes of plots by dispersion indices, and spatial autocorrelation. Population densities (D) varied from 0.356 to 2.270, with a mean of 1.527. The values of dispersion indices ( at Mt. Ahop were lower than 1 at six plots ($2m{\times}2m$, $2m{\times}4m$, $4m{\times}4m$, $4m{\times}8m$, $8m{\times}8m$, and $8m{\times}16m$), but the two large plots ($16m{\times}16m$ and $16m{\times}32m$) were higher than 1. Thus, the aggregation indices ( were negative at Mt. Ahop, which indicates a uniform distribution. The two large plots ($16m{\times}16m$ and $16m{\times}32m$) had positive CIs. However, the values were not large (0.009 for the $16m{\times}16m$ plot and 0.038 for the $16m{\times}32m$ plot). The mean crowding ($M^{\ast}$) and patchiness index (PAI) showed positive values for all plots.

• Microbiology and Biotechnology Letters
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• v.36 no.1
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• pp.28-33
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• 2008

The expression vector (pWHM3-TR1R2) for sprT gene encoding Streptomyces griseus trypsin (SGT) followed by two regulatory genes, sgtR1 and sgtR2, was introduced into Streptomyces lividans TK24 and Streptomyces griseus IFO 13350. Various media with different compositions were used to maximize the productivity of SGT in the recombinant trains. he SGT productivity was best when the transformant of S. lividans TK24 was cultivated in R2YE medium (0.74 unit/mL) at 5 days of cultivation. C5/L (0.66 unit/mL) medium also gave a good productivity, but Livid (0.08 unit/mL) and NDSK (0.06 unit/mL) yielded poor productivities. S. griseus IFO 13350/pWHM3-TR1R2 produced SGT by 1.518 unit/mL (C5/L), 1.284unit/mL (R2YE),0.932 unit/mL (NDSK), and 0.295 unit/mL (Livid) at 7 days of cultivation, which was much higher than those from S. lividans TK24/TR1R2. The SGT protein was purified from the culture broth of S. griseus IFO 13350/pWHM3-TR1R2 in C5/L to homogeneity via ammonium sulfate fractionation, and CM-sepharose and SP-sepharose column chromatographies. The specific activity of purified SGT was 69,252 unit/mg, and the final purification fold and recovery yield were 6.5 and 1.4%, respectively.