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Influence of Caffeine on Dopamine D$_1$and D$_2$Receptor mRNAs Expression in Rat Brain (도파민 D$_1$과 D$_2$수용체 mRNAs의 발현에 미치는 카페인의 영향)

  • 김근양;신지혜;김명옥
    • Journal of Life Science
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    • v.11 no.1
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    • pp.1-7
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    • 2001
  • The caffeine intake cause a local or wide ranges of convulsion and it is associated with release of dopamine (DA) receptors into the brain striatum. However, the effect of caffeine addiction on expression of DA receptors gene in the rat caudate-putamen (CPu), nucleus accumbens (NAc), and olfactory tubercle (OTu) has not been elucidated. In this study, we examined the influence of caffeine addiction on DA D $_1$and D$_2$receptor mRNAs after the treatment of caffeine for four weeks. Using the specific antisense ribo-probes for DA D$_1$and D$_2$receptor cDNAs, in situ hybridization was performed on the CPu, NAc, and OTu of the adult male Sprague Dawely rats. In caffeine-treated group, DA D$_1$and D$_2$receptor mRNAs were highly increased in CPu, NAc, and OTu. The expression density of DA D$_1$receptor mRNAs were 2.52${\pm}$1.40 (CPu), 2.78${\pm}$1.69 (NAc), and 3.91${\pm}$1.28 (OTu) in control group and 7.76${\pm}$2.09 (CPu), 4.2 ${\pm}$1.85 (NAc), and 8.21${\pm}$1.72 (OTu) in caffeine-treated group. The expression density of DA D$_2$receptor mRNA was 2.32${\pm}$1.52 (CPu), 2.63${\pm}$2.11 (NAc), and 3.61${\pm}$1.43 (OTu) in control group, and 6.41${\pm}$1.82 (CPu), 6.89${\pm}$1.32 (NAc), and 6.82${\pm}$1.18 (OTu) in caffeine-treated group. DA D$_1$receptor mRNA was higher expressed than DA D$_2$ receptor mRNA in CPu and NAc. These results suggest that caffeine reacts as a upregulator of the expression of DA D$_1$and D$_2$receptor mRNA among the neurotransmitters.

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Effects of Soil EC on Seed Germination, Seeding Growth, Initiation of Female Flower and Fruit Growth of Cucumber (Cucumis sativus L.) Plants (토양 EC가 오이의 종자발아, 유묘의 생장, 자화착생 및 과실비대에 미치는 영향)

  • Chung, Hee-Don;Jang, In-Suk;Choi, Young-Jun
    • Horticultural Science & Technology
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    • v.19 no.4
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    • pp.495-500
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    • 2001
  • Seed germination, seedling growth, plant establishment after transplanting, set of female flowers, and subsequent fruit growth were examined in cucumbers grown in soils having varying electrical conductivities (EC) of $1.0-5.0dS{\cdot}m^{-1}$ adjusted by adding different amount of a commercial compound fertilizer (N : $P_2O_5$ : $K_2O$ = 21 : 17 : 17). Slower seed germination was recorded in soils having EC value of $3.0dS{\cdot}m^{-1}$ or higher and little or no germination took place in soils with EC $4.0dS{\cdot}m^{-1}$ until 8 days after sowing. Suppression of seedling growth was apparent in soils having EC level of $2.5dS{\cdot}m^{-1}$. All seedlings died eventually at EC level of $3.5dS{\cdot}m^{-1}$. Leaf color became progressively dark green with the increase in EC from 1.0 to $3.0dS{\cdot}m^{-1}$. The first female flower was formed at higher node and the number of days required for the first flowering of female flower increased with the increase in EC values up to $3.0dS{\cdot}m^{-1}$. Occasionally, leaf yellowing and self-topping symptoms appeared in plants grown in soils having EC level of $3.0dS{\cdot}m^{-1}$.

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APPROXIMATION OF THE QUEUE LENGTH DISTRIBUTION OF GENERAL QUEUES

  • Lee, Kyu-Seok;Park, Hong-Shik
    • ETRI Journal
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    • v.15 no.3
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    • pp.35-45
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    • 1994
  • In this paper we develop an approximation formalism on the queue length distribution for general queueing models. Our formalism is based on two steps of approximation; the first step is to find a lower bound on the exact formula, and subsequently the Chernoff upper bound technique is applied to this lower bound. We demonstrate that for the M/M/1 model our formula is equivalent to the exact solution. For the D/M/1 queue, we find an extremely tight lower bound below the exact formula. On the other hand, our approach shows a tight upper bound on the exact distribution for both the ND/D/1 and M/D/1 queues. We also consider the $M+{\Sigma}N_jD/D/1$ queue and compare our formula with other formalisms for the $M+{\Sigma}N_jD/D/1$ and M+D/D/1 queues.

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Physiological Characteristics of Resistant Starch (HI-MAIZE DIET) Fortified with Other Dietary Fiber Components (식이섬유의 기능이 강화된 저항전분 (HI-MAIZE DIET)의 생리적 특성)

  • Choi, Yang-Mun;Oh, Sung-Hoon;Yu, Kwang-Won;Shin, Kwang-Soon;Ra, Kyung-Soo;Park, Chul-Soo;Kim, Kyung-Mi;Suh, Hyung-Joo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.3
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    • pp.351-355
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    • 2005
  • This study was performed to investigate the influences of resistant starch (HM: HI-MAIZE) and HM-D (HI-MAIZE DIET) fortified with D-factor (consisted of Psyliium husk, polydextrose and hydrocitric acid) on the glucose and bile acid absorption and production of short chain fatty acids (SCFA). HM-D absorbed more glucose and bile acid than did HM. The glucose transport of HM and HM-D against dialysis membrane showed 77% and 68% for 4h, respectively. After 24h, bile acid transport of HM and HM -D showed 65% and 62.3%, respectively. The HM and HM-D produced 217.8 mM and 264.0mM of SCFA, respectively. The production of butyric acid in HM-D (32.7mM) showed higher than that of HM (26.9mM). The addition of D-factor to HM increased the physiological function of dietary fiber through the glucose and bile acid absorption and production of SCFA.

Seasonal Change of Rice-mediated Methane Emission from a Rice Paddy under Different Water Management and Organic Amendments (물 관리와 유기물 시용이 다른 논에서 벼 식물체를 통한 메탄 배출의 계절변화)

  • Shin, Yong-Kwang;Lee, Yang-Soo;Ahn, Jong-Woong;Koh, Mun-Hwan;Eom, Ki-Cheol
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.1
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    • pp.41-49
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    • 2003
  • Methane emission was measured in a rice paddy under different water management and organic amendments. Methane emission from planted chambers and unplanted chambers was monitored to evaluate the rice-mediated methane emission. In flooding methane emission from planted chambers with NPK, NPK(+P), was $0.174g\;CH_4\;m^{-2}\;d^{-1}$ while that from unplanted chambers with NPK, NPK(-P), was $0.046g\;CH_4\;m^{-2}\;d^{-1}$ Methane emission from planted chambers with rice straw compost amendment, RSC(+P), was $0.214g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chambers with rice straw compost amendment, RSC(-P), was $0.076g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from planted chambers with rice straw amendment in Fehruary, RS2(+P), was $0.328g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chambers with rice straw amendment in February, RS2(-P), was $0.1g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from planted chambers with rice straw amendment in May, RS5(+P), was $0.414g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chamhers with rice straw amendment in May, RS5(-P), was $0.187g\;CH_4\;m^{-2}\;d^{-1}$. In intermittent irrigation methane emission from NPK(+P) was $0.115g\;CH_4\;m^{-2}\;d^{-1}$, while that from NPK(-P) was $0.041g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RSC(+P) was $0.137g\;CH_4\;m^{-2}\;d^{-1}$, while that from RSC(-P) was $0.06g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RS2(+P) was $0.204g\;CH_4\;m^{-2}\;d^{-1}$, while that from RS2(-P) was $0.09g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RS5(+P) was $0.273g\;CH_4\;m^{-2}\;d^{-1}$, while that from RS5(-P) was $0.13g\;CH_4\;m^{-2}\;d^{-1}$. Methane transport via rice plant under flooding for NPK plot, RSC plot, RS2 plot and RS5 plot was 73.6%, 64.5%, 69.5% and 54.8%, respectively, and mean was 65.6%. Methane transport via rice plants under intermittent irrigation for NPK plot, RSC plot, RS2 plot and RS5 plot was 64.3%, 59.2%, 55.9% and 52.4%, respectively, and mean was 58.0%.

Production of D-Lactic Acid from DL-Lactonitrile by Pseudomonas sp. (Pseudomonas sp.에 의한 DL-Iactonitrile로부터 D-lactic acid의 생산)

  • 김현수;황인균;정남현;방원기
    • Microbiology and Biotechnology Letters
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    • v.30 no.4
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    • pp.373-379
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    • 2002
  • By using DL-acetonitrile as enzyme inducer, 90 bacteria were isolated from a field soil. Among the isolated strains, the strain WJ-003 showed the highest activity for production of D-lactic acid from DL-lactonitrile, and was partially identified as Pseudomonas sp. The production condition of D-lactic acid from DL-lactonitrile using resting cells as an enzyme source was optimized as follows: the reaction mixture contained 10 mM of DL-lactonitrile, 20 g of wet cells in 11 of 20 mM potassium phosphate buffer (pH 7.0) and the reaction was carried out at $30^{\circ}C$. After 18 h of reaction, 0.843 g/l of D-lactic acid was produced which corresponded to a conversion ratio of 93.7% and an optical purity of 99.8%. Additionally, when 10 mM of DL-lactonitrile was added once more to the reaction mixture at 14 h, 1.64 g/1 of D-lactic acid was produced after 28 h. In this experiment, the conversion ratio was 91.1% and optical purity 99.8%.

A Study on V.M.D(Visual Merchandising Design) Environment of Mobile Telecommunication Company Store (이동통신 회사 매장의 V.M.D(Visual Merchandising Design) 환경에 관한 연구)

  • Kim, Jong Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.4
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    • pp.1589-1594
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    • 2013
  • V.M.D(Visual Merchandising Design) covers every space, environment, and visually stimulating factor. This includes not only the architectural appearance but interior, graphic sign boards, window display, layout, store appliances, lighting, and even the looks and service attitude that sales employees have and show to customers. V.M.D aims to display merchandise in multiple ways; to heighten brand images; to steadily lure many customers to the store. One of the characteristics that V.M.D has is, that this abandons the previous design concept which solely puts stress on luxurious interior-focused display; that this displays merchandise in a rational and systemic way and induces customers to buy products. In this regard, the objective of this study is to study brand perception and effect of V.M.D displayed in the stores, on customers, and thereby to present the concepts of V.M.D, suitable for display in the stores, which can be used continuously in the future.

Effect of the Vessel Vibration Noise to the Underwater Ambient Noise (선박진동소음이 해중환경소음에 미치는 영향에 관한 연구)

  • 박중희
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.23 no.4
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    • pp.163-168
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    • 1987
  • The underwater observation of the ambient noise and the noise generated by the engine revolution in a ship was carried out in July to August, 1984, 1985 and 1987, near around some ports and in the Eastern Sea of Korea. Vertical distribution of the sound pressure of both noises were observed and the spectrum characteristics were analysed and compared. The results obtained are summarized as follows: 1. Sound pressure level of the ambient noise at 5m deep layer in calm sea condition (wind speed 0-2m/s) near around the ports were observed as 108dB at the eastern part of Pusan port, 106dB at the southern part of Pusan port and 101dB at Kuryongpo port. It shows that the level near around the large port which contains much noisy resources is higher than the small port. The level at 5m deep layer in the open sea, in the mid-region between Korean Peninsula and Ulnung Island was observed as 100dB. It mean that the level in the open sea is lower than that around the ports. The level at 20m and 70m deep layer were 1-2dB lower than that at 5m deep layer, and that at deeper layer than 100m was almost constantly 100dB around. 2. Sound pressure level of the ambient noise at 5m deep layer in windy open sea condition (wind speed 10-15m/s) was 108dB, and was gradually decreased in accordance with the increase of depth with representing 100dB at 70m deep layer and that at deeper layer was almost constantly 100dB. The level of the noise generated by engine revolution was 146, 125, 112, 110, 104dB at 5, 50, 100, 150 and 200m deep layer respectively. It means that the level decrease with the depth. 3. Spectrum level of the ambient noise at 5m deep layer with the frequency band of 10 Hz, 100 Hz, 1 KHz, 10 KHz, in the windy sea condition were 86, 75, 61, 32dB respectively and the level of the noise generated by engine revolution was 105, 95, 86, 55dB respectively. It means that the latter are about 20dB higher than the former. The level of the former at 200m deep layer was 80, 68, 47, 26dB and the latter 82, 70, 59, 31dB. It means that the latter are about 4dB higher than the former.

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DERIVATION MODULES OF GROUP RINGS AND INTEGERS OF CYCLOTOMIC FIELDS

  • Chung, I.Y.
    • Bulletin of the Korean Mathematical Society
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    • v.20 no.1
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    • pp.31-36
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    • 1983
  • Let R be a commutative ring with 1, and A a unitary commutative R-algebra. By a derivation module of A, we mean a pair (M, d), where M is an A-module and d: A.rarw.M and R-derivation, i.e., d is an R-linear mapping such that d(ab)=a)db)+b(da). A derivation module homomorphism f:(M,d).rarw.(N, .delta.) is an A-homomorphism f:M.rarw.N such that f.d=.delta.. A derivation module of A, (U, d), there exists a unique derivation module homomorphism f:(U, d).rarw.(M,.delta.). In fact, a universal derivation module of A exists in the category of derivation modules of A, and is unique up to unique derivation module isomorphisms [2, pp. 101]. When (U,d) is a universal derivation module of R-algebra A, the A-module U is denoted by U(A/R). For out convenience, U(A/R) will also be called a universal derivation module of A, and d the R-derivation corresponding to U(A/R).

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The Biochemical Characterization of D-Hydroxyisovalerate Dehydrogenase, a Key Enzyme in the Biosynthesis of Enniatins

  • Lee, Chan; Zocher, Rainer
    • BMB Reports
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    • v.29 no.6
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    • pp.493-499
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    • 1996
  • The biochemical properties of purified D-hydruxyisovalerate dehydrogenase from Fusarium sambucinum was elucidated. D-Hydroxyisovalerate dehydrogenase produced solely D-hydroxyisovalerate from 2-ketoisovalerate. The isoelectric point of the purified enzyme was 7.0. The enzyme was highly specific with 2-ketoisovalerate ($K_{m}=0.188$ mM, $V_{max}=8.814$ mmol/min mg) and 2-keto-3-methyl-n-valerate ($K_{m}=0.4$ mM, $V_{max}=1.851$ mmol/min mg) for the reductive reaction. This was also seen by comparing D-hydroxyisovalerate ($K_{m}=1.667$ mM, $V_{max}=0.407$ mmol/min mg) and D-hydroxy-3-methyl-n-valerate ($K_{m}=6.7$ mM, $V_{max}=0.648$ mmol/min mg) for the oxidative reaction. Thiol blocking reagents, such as iodoacetamide, N-ethylmaleimide and p-chloromecuribenzoate inhibited about 80% of enzyme activity at 0.02 mM, 50 mM and 50 mM, respectively. The enzyme activity was also inhibited by the addition of 0.1 mM of various metal ions, such as $Fe^{2+}$ (67%), $Cu^{2+}$ (88%), $Zn^{2+}$ t (76%) and $Mg^{2+}$ (9%). The enzyme was stable over three months in 50 mM potassium phosphate buffer (pH 5~7) at $-80^{\circ}C$. However the purified enzyme lost 30% of its activity in the same buffer after 24 h at $4^{\circ}C$. The studies about thermal inactivation of D-hydroxyisovalerate dehydrogenase exhibit 209.2 kJ/M of activation enthalpy and 0.35 kJ/mol K of activation entropy.

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