• 식물병연구
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• 제19권4호
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• pp.294-299
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• 2013

새로운 채소류를 뿌리혹선충이 감염된 시설재배지에 윤작작물로 추천하기 위하여 7과 13속, 25종, 총 39품종을 고구마뿌리혹선충과 땅콩뿌리혹선충을 대상으로 저항성을 검정하였다. 2종류의 뿌리혹선충에 모두 감수성인 것은 비름과(Amaranthaceae)의 아마란서스와 근대, 낙규과(Basellaceae)의 바우세, 피나무과(Tiliaceae)의 모로헤이야, 메꽃과(Convolvulaceae)의 공심채, 십자화의 청경채, 홍채태(Brassica campestris var. chinensis), 다채(B. campestris var. narinosa), '다청채'(B. campestris var. chinensis x narinosa), 갓, 곱슬겨자(B. juncea), 교나(B. juncea var. laciniate), 채심(B. rapa subsp. arachinenesis), 가이란(B. oleracea var. alboglabra), 로케트샐러드(Eruca sativa), 국화과(Asteraceae)의 쑥갓(Chrysanthemum coronarium), 엔다이브(Cichorium endivia), 아티쵸크(Cynara cardunculus var. scolymus), 상추(Lactuca sativa)였다. M. incognita에는 저항성이나 M. arenaria에는 감수성인 것은 십자화과의 케일(B. oleracea) cv. 맛짱, 콜라비(B. oleracea var. gongyloides) cv. 적콜라비, 치커리(C. intybus) cv. 적치커리였다. 두 종류의 뿌리혹선충에 모두 저항성인 것은 치커리(C. intybus) cv. 슈가로프, 구루모, 통콘네로네(radichio treviso), 십자화과의 케일(B. oleracea) cv. 만추콜라드, 슈퍼맛짱 브로콜리(B. oleracea italica), 잎브로콜리(B. oleracea var. botrytis italiana), 꿀풀과(Lamiaceae)의 차즈기, 들깨였다. 2종류의 뿌리혹선충에 모두 저항성인 채소류는 뿌리혹선충이 감염된 시설재배지에서 부가가치가 높은 저항성 윤작작물로 사용이 가능할 것이다.

• 생명과학회지
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• 제17권6호통권86호
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• pp.772-777
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• 2007

식물의 루이신 생합성에 관여하는 3-isopropylmalate isomerase (IPMI) (EC 4.2.1.33) 효소의 소단편을 암호화는 Leucine D유전자를 벼로부터 분리하고 OsLeuD유전자로 명명하였다. OsLeuD유전자는 257개의 아미노산을 암호화하고 있으며 cyanobacteria의 IPMI 단백질과는 약58% 그리고 green sulfur bacteria들의 IPMI 단백질과는 약48%의 상동성을 갖고 있었다. 벼의 OsLeuD 유전자는 japonica벼 (Oryza sativa L.)의 2번 염색체의 26.45 Mb의 위치로서 109.3 cM 거리에 좌위하고 있었다. OsLeuD유전자는 잎과 성숙하는 종자에 많이 발현이 되었으므로 대사가 급증하는 발생단계 에 발현이 조절되는 것으로 여겨진다. OsLeuD유전자와 단백질은 균류와 yeast 보다 광합성 박테리아의 유전자와 높은 동질성을 보이는 것으로 보아 OsLeuD유전자는 식물의 엽록체 유전자 genome 에서 기원하여 핵 genome으로 이동 진화된 유전자로 추측된다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권10호
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• pp.1425-1434
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• 2011

This experiment was designed to evaluate the effects of the processing method of common vetch seed (CVS) (Vicia sativa) on laying performance, egg quality, metabolic parameters and liver histopatology during the peak production period in hens. Lohman layers, 46 wk of age in 6 replicate cages each containing 4 hens, were allocated randomly to one of four dietary treatments. Diets were control (C) diet containing no common vetch and experimental diets containing 25% raw common vetch (RCV), 25% soaked in water for 72 h with exchange of water every 24 h (SCV) and 25% soaked&boiled at $100^{\circ}C$ for 30 minute common vetch (SBCV). Inclusion of RCV into the diet deteriorated all laying performance variables. SCV did not alleviate the adverse effect of raw common vetch on feed intake, egg weight, feed conversion, final weight and weight change. SCV partially alleviated egg production (p<0.001). SBCV diminished the adverse effect on feed intake, egg weight, feed conversion, final weight and weight change compared to raw vicia sativa (p<0.001). No significant difference was detected between SBCV and the control group in terms of egg production, feed conversion, final weight and weight change. Regardless of the processing method, all the common vetch groups had lower shell strength compared to the control group. Haugh units did differ between all groups (p<0.001). Inclusion of RCV and SCV into the basal diet decreased triglyceride, cholesterol, total protein and serum glucose concentrations (p<0.001). Hovewer, inclusion of SBCV into the basal diet increased these parameters. Liver samples were stained with Hematoxylin and eosin (HE) and evaluated by light microscopy. A biopsy of native liver tissue was used as a control. No histopathologic finding was present in the control group. Raw V. sativa compared with the control caused lipid accumulations in hepatocytes, severe congestion of hepatic blood vessels, inflammation, increased numbers of Kupffer cells and sinusoidal dilatations. Whereas, the livers from groups given treated V. sativa showed only different degrees of sinusoidal dilatations. Findings from the present study point out the risk of increased hepatic damage due to use of raw Vicia sativa. Increasing treatment of V. sativa lead to a decrease of liver damages. Inclusion of raw and soaked vetch seeds in rations affected adversely all parameters examined in laying hens. But alleviation was observed when soaked and boiled vetch seeds (SBCV) were fed. The results of these experiments indicated that soaked&boiled Vicia sativa seeds may safely be used at a 25% level in rations of laying hens.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.38-38
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• 2017

Water stress obstructs rice growth mainly by oxidative damage in biological cells to cause a reduction of leaf photosynthesis and evapotranspiration processes. In this study, exogenous application of vanillic acid (VA) and p-hydroxybenzoic acid (PHBA) to improve drought tolerance of two Oryza sativa cultivars, Q2 and Q8 was tested. The drought evaluation based on leaf phenotypes to show that both Q2 and Q8 resulted in remarkable water-stress tolerance induced by leaf spraying pretreatment of mixed solution of $50{\mu}M\;VA+50{\mu}M\;PHBA$. The mixtures of $25{\mu}M\;VA+25{\mu}M\;PHBA$ and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treated on Q2 and Q8 in water deficit condition also indicated that total phenols, total flavonoids, and DPPH radical scavenging activity were significantly greater their controls. In general, the accumulation of individual phenolic acids was increased in exogenous phenolic treatments, as compared with controls. Particularly, Q2 obtained a considerable amount of endogenous PHBA after application of $50{\mu}M\;VA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ (0.18 mg/g DW, 0.71 mg/g DW, and 1.41 mg/g DW, respectively); and a negligible content of VA (0.003 mg/g DW) appeared uniquely in the treatment of $50{\mu}M\;VA$. Similarly, Q8 also absorbed a significant quantity of PHBA in $50{\mu}M\;PHBA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treatments (0.15 mg/g DW, 0.15 mg/g DW, and 0.22 mg/g DW, respectively). In addition, the spraying $50{\mu}M\;PHBA$ and $25{\mu}M\;VA+25{\mu}M\;PHBA$ on Q8 leaves induced similar amount of drought tolerance of Q2 and Q8 were improved, paralleled with the increased amounts of endogenous phenolics revealed that VA and PHBA played an important role to enhance drought tolerance in rice.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.33-33
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• 2017

Water stress obstructs rice growth mainly by oxidative damage in biological cells to cause a reduction of leaf photosynthesis and evapotranspiration processes. In this study, exogenous application of vanillic acid (VA) and p-hydroxybenzoic acid (PHBA) to improve drought tolerance of two Oryza sativa cultivars, Q2 and Q8 was tested. The drought evaluation based on leaf phenotypes to show that both Q2 and Q8 resulted in remarkable water-stress tolerance induced by leaf spraying pretreatment of mixed solution of $50{\mu}M\;VA+50{\mu}M\;PHBA$. The mixtures of $25{\mu}M\;VA+25{\mu}M\;PHBA$ and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treated on Q2 and Q8 in water deficit condition also indicated that total phenols, total flavonoids, and DPPH radical scavenging activity were significantly greater their controls. In general, the accumulation of individual phenolic acids was increased in exogenous phenolic treatments, as compared with controls. Particularly, Q2 obtained a considerable amount of endogenous PHBA after application of $50{\mu}M\;VA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ (0.18 mg/g DW, 0.71 mg/g DW, and 1.41 mg/g DW, respectively); and a negligible content of VA (0.003 mg/g DW) appeared uniquely in the treatment of $50{\mu}M\;VA$. Similarly, Q8 also absorbed a significant quantity of PHBA in $50{\mu}M\;PHBA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treatments (0.15 mg/g DW, 0.15 mg/g DW, and 0.22 mg/g DW, respectively). In addition, the spraying $50{\mu}M\;PHBA$ and $25{\mu}M\;VA+25{\mu}M\;PHBA$ on Q8 leaves induced similar amount of VA (0.04 mg/g DW). Meanwhile, there were no trace of VA and PHBA found in controls. The levels of drought tolerance of Q2 and Q8 were improved, paralleled with the increased amounts of endogenous phenolics revealed that VA and PHBA played an important role to enhance drought tolerance in rice.

• 한국작물학회지
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• 제51권4호
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• pp.362-368
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• 2006

A pot experiment was conducted to investigate the effects of soil burial depth on seedling emergences of rice (Oryza sativa) and Echinochloa spp. and to model such effects for mathematical prediction of seedling emergences. When the Gompertz curve was fitted at each soil depth, the parameter C decreased in a logistic form with increasing soil depth, while the parameter M increased in an exponential form and the parameter B appeared to be constant. The Gompertz curve was combined by incorporating the logistic model for the parameter C, the exponential model for the parameter M, and the constant for the parameter B. This combined model well described seedling emergence of rice and Echinochloa species as influenced by soil burial depth and predicted seedling emergence at a given time after sowing and a soil burial depth. Thus, the combined model can be used to simulate seedling emergence of crop sown in different soil depths and weeds present in various soil depths.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.202.1-202
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• 2003

No Abstract, See Full Text

• 한국잡초학회지
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• 제9권3호
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• pp.245-249
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• 1989

식물생장(植物生長) 억제제(抑制劑)인 butachlor의 작용기구(作用機構)를 구명(究明)하기 위하여 본(本) 제초제(除草劑)가 생장기본요소(生長基本要素)인 세포분열(細胞分裂) 및 단백질(蛋白質) 합성(合成) 억제(抑制)에 미치는 영향(影響)은 다음과 같다. 1. Butachlor의 세포분열(細胞分裂) 억제(抑制) 효과(效果)는 18시간(時間) 처리후(處理後) $10^{-6}M$은 9.3%, $10^{-5}M$은 28.2%의 억제(抑制)를 보였으며, 특히 $10^{-3}M$ 농도(濃度)에서는 81.6%의 현저한 억제(抑制)를 보임으로서 본(本) 제초제(除草劑)는 농도(濃度)가 높아질수록 세포분열(細胞分裂) 억제효과(抑制效果)도 증가(增加)하였다. 2. 단백질(蛋白質) 억제(抑制) 효과(效果)는 18시간(時間) 처리후(處理後) $10^{-4}M$ 농도(濃度)에서 22.9%, $10^{-3}M$은 34.1%의 억제효과(抑制效果)를 보였다. 또한 24시간(時間) 처리후(處理時) $10^{-4}M$은 34.3%로 로서 18시간(時間) 처리후(處理後)의 $10^{-3}M$과 거의 같은 억제효과(抑制效果)를 보이고 있다. 이와같이 butachlor 는 농도(濃度)와 처리시간(處理時間)이 길어짐에 따라서 단백질(蛋白質) 농도(濃度)의 증가(增加)를 보였다. 3. Butachlor 처리(處理)는 전체적인 단백질(蛋白質) 합성(合成)을 억제(抑制)하였고 특히 분자량 16, 18, 30, 43 그리고 43.5KD의 단백질(蛋白質)을 억제(抑制)하는 것으로 나타났으며 귀리(Avena sativa L.)의 root tip은 100,000 이하의 polypeptide subunit의 단백질로 구성(構成)되었다.

• BMB Reports
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• 제41권11호
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• pp.771-777
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• 2008

Calmodulin (CaM) proteins, members of the EF-hand family of $Ca^{2+}$-binding proteins, represent important relays in plant calcium signals. Here, OsCam1-1 was isolated by PCR amplification from the rice genome. The gene contains an ORF of 450 base pairs with a single intron at the same position found in other plant Cam genes. A promoter region with a TATA box at position-26 was predicted and fused to a gus reporter gene, and this construct was used to produce transgenic rice by Agrobacterium-mediated transformation. GUS activity was observed in all organs examined and throughout tissues in cross-sections, but activity was strongest in the vascular bundles of leaves and the vascular cylinders of roots. To examine the properties of OsCaM1-1, the encoding cDNA was expressed in Escherichia coli. The electrophoretic mobility shift when incubated with $Ca^{2+}$ indicates that recombinant OsCaM1-1 is a functional $Ca^{2+}$-binding protein. In addition, OsCaM1-1 bound the CaMKII target peptide confirming its likely functionality as a calmodulin.

• 한국초지조사료학회지
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• 제40권3호
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• pp.190-195
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• 2020

Cadmium (Cd) toxicity is a serious limitation for agricultural production. In this study, we explored tolerance mechanism associated with Cd toxicity tolerance in alfalfa plants. We used three distinct alfalfa cultivars M. sativa cv. Vernal, M. sativa cv. Zhung Mu, and M. sativa cv. Xing Jiang Daye in this study. Cd showed declined chlorophyll score in Xing Jiang Daye compared with Zhung Mu and Vernal. No significant change observed among the cultivars for root and shoot length. Atomic absorption spectroscopy analysis demonstrated a significant accumulation of Cd, Fe, S and PC in distinct alfalfa cultivars. However, Zhung Mu and Xing Jiang Daye declined Cd accumulation in root, where Fe, S and PC incremented only in Zhung Mu. It suggests that excess Cd in Zhung Mu possibly inhibited in root by the increased accumulation of Fe, S and PC. This was further confirmed by the response of Fe (MsIRT1) and S transporters (MsSULTR1;2 and MsSULTR1;3), and MsPCS1 genes associated with Fe, S and PC availability and translocation in roots and shoots. It suggests that specially the transcript signal inducing the responses to adjust Cd especially in Zhung Mu. This finding provides the essential background for further molecular breeding program for forage crops.