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Ethanol Fermentation of Fusant between Heterologous Transformant of Saccharomyces cerevisiae and Candida tropicalis in Pilot Scale (Pilot Scale에서의 Fusant의 Ethanol 발효)

  • Seu, Jung-Hwn;Kim, Young-Ho;Lee, Soon-Mo;Bang, Byung-Ho
    • Microbiology and Biotechnology Letters
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    • v.17 no.1
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    • pp.14-18
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    • 1989
  • As the final experiment to assess the possibility of industrial application of FSC14-75, ethanol productivity from liquefied sweet potato starch was examined in a pilot scale of 300 liters. FSC14-75 produced 6.6%(v/v) of ethanol from 13.3% of liquefied sweet potato starch in 8 days, and the residual sugar was 3.15%. The corresponding efficiency was 70% of the theoretical maximum. Since we could isolate unicellular cell and flocculent cell from the fermentation broth, we designated them FSC14-75(S) and FSC14-75(F), respectively. We investigated ethanol productivity of FSC14-75(F) compared with that of FSC14-75(S) from liquefied potato starch in a mini·tar tormentor scale of 2.5 liters. FSC14-75(F) was found more favorable than the counterpart in terms of ethanol productivity, and produced 8.1%(v/v) of ethanol from 15% of liquefied potato starch with an efficiency of 75%. In a pilot scale fermentation with 15% of liquefied sweet potato starch, ethanol productivity of FSC14-75(F) reached maximum level of 7.7%(v/v) after 8 days, and the residual sugar was 1.9%. However, the ethanol productivity was not enhanced by a supplementary addition of Thermamyl to the fermentation broth after sterilization.

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Nickel Catalysts Supported on Ash-Free Coal for Steam Reforming of Toluene (무회분탄에 분산된 니켈 촉매의 톨루엔 수증기 개질)

  • PRISCILLA, LIA;KIM, SOOHYUN;YOO, JIHO;CHOI, HOKYUNG;RHIM, YOUNGJOON;LIM, JEONGHWAN;KIM, SANGDO;CHUN, DONGHYUK;LEE, SIHYUN
    • Transactions of the Korean hydrogen and new energy society
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    • v.29 no.6
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    • pp.559-569
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    • 2018
  • Catalytic supports made of carbon have many advantages, such as high coking resistance, tailorable pore and surface structures, and ease of recycling of waste catalysts. Moreover, they do not require pre-reduction. In this study, ash-free coal (AFC) was obtained by the thermal extraction of carbonaceous components from raw coal and its performance as a carbon catalytic support was compared with that of well-known activated carbon (AC). Nickel was dispersed on the carbon supports and the resulting catalysts were applied to the steam reforming of toluene (SRT), a model compound of biomass tar. Interestingly, nickel catalysts dispersed on AFC, which has a very small surface area (${\sim}0.13m^2/g$), showed higher activity than those dispersed on AC, which has a large surface area ($1,173A/cm^2$). X-ray diffraction (XRD) analysis showed that the particle size of nickel deposited on AFC was smaller than that deposited on AC, with the average values on AFC ${\approx}11nm$ and on AC ${\approx}23nm$. This proved that heteroatomic functional groups in AFC, such as carboxyls, can provide ion-exchange or adsorption sites for the nano-scale dispersion of nickel. In addition, the pore structure, surface morphology, chemical composition, and chemical state of the prepared catalysts were analyzed using Brunauer-Emmett-Taylor (BET) analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, and temperature-programmed reduction (TPR).

Manufacture of Activated Carbon Using Livestock Manure and it's Odor Absorptiveness (축분을 이용한 활성탄소 제조와 이의 악취 흡착성 분석)

  • Choi, H.C.;Song, J.I.;Kwon, D.J.;Kwag, J.H.;Yan, C.B.;Yoo, Y.H.;Park, Young-Tae;Park, K.S.;Park, D.K.;Kim, Y.K.
    • Journal of Animal Environmental Science
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    • v.13 no.3
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    • pp.211-218
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    • 2007
  • This study was carried out to develop the technique for manufacturing activated carbon from livestock manure and to analyse it's odor absorptiveness. Each of layer manure(LM), litter from broiler house(BL) and litter from dairy barn(DL), compost from layer manure(LC) and pig manure(PC), and coconut shell(CS) was used as a raw material. Activated carbon by grinding the raw material, adding the coal tar as a binder, palletizing, drying, heating with $N_2$ gas at $400^{\circ}C$ for 1 hour, activating by reaction with steam at a temperature of $750^{\circ}C$ for 1 hour. Moisture contents of raw material was 44.9% in layer compost, 71.9% in layer manure, 24.4% in broiler litter, 47% in pig manure compost and 33.9% in dairy litter. Volatile matter in layer compost, layer manure, broiler litter, pig manure compost and dairy litter was 18.8%, 31.0%, 49.8%, 22.3% and 11.6%, respectively. Surface area(BET) of activated carbon from layer compost, layer manure, broiler litter, pig manure compost, dairy litter and coconut shell was 259.8, 209.8, 63.5, 442.3, 812.9 and $1,040\;m^2/g$, respectively. Activated carbon made by livestock manure or litter were examined with scanning electron microscope, and micropore was a type of sponge like particles honeycombed with chambers. Pore size of activated carbon was ranged from 0.39 to $5.02\;{\AA}$, but coconut shell was $0.30\;{\AA}$. Iodine absorptiveness of activated carbon from livestock manure was $530{\sim}580mg/g$. But activated carbon made by coconut shell was 1000 mg/g. Each activated carbon could absorb odor compound very well. Absorptiveness of activated carbon from layer manure for hydrogen sulfide and trimethyl amino was 74.5% and 73.9% at the accumulated flux of 60,000 ml, but, in the case of ammonia was only 15.2% at the accumulated flux of 10,000 ml

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Effect of Plant Growth Regulators in Minimizing Low Temperature Stress in Rice(Oryza sativa L.) (식물생장조절제(植物生長調節劑) 처리(處理)가 벼(Oryza sativa L.) 냉해경감(冷害輕減)에 미치는 영향(影響))

  • Yoon, Jong Yeon;Lee, Sang Chul;Choi, Jang Su
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.43-54
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    • 1993
  • This experiment was conducted at Andong county cropping season to investigate rice response and consequent changes in the physiological activities and agronomic characters of rice as affected by growth regulator treatments, and to determine how low temperature controls some plant growth mechanisms. Plant growth regulators, particularly ABA, applied to 15 DAT regardless of concentration, gave higher tar plant height and tiller number after 10days of low temperature treatment than did the untreated plant. However, the differences in plant height and tiller number among treatments diminished to plant growth. Chlorophyll content seemed to be enhanced by growth regulators under low temperature condition. The 10-day treatment of low temperature decreased chlorophyll content by more than 17% compared with the untreated control. The oxidizing activity of roots decreased sharply to 52.2a after 10 days in low temperature condition. On the other hand, ABA at concentration of $10^{-4}M$, highly attains oxiding activity of roots. Generally, plant growth regulators applied at 15 DAT under low temperature reduced grain yield more than did the untreated control which can be attributed to decreased yield components. When growth regulators were applied to booting stage, the tiller number did not show significant difference, whereas the significant difference in culm length at harvest was observed in all treatment as a result of different concentration of growth regulators. The growth regulators generally exhibited a significant effect on yield component except on panicle length. Grain yield of low temperature treated plot at booting stage was significantly influenced by application of growth regulators. This was due to decreased number of spikelet and low filled spikelet percentage. However, TIBA treatment at booting stage showed no significant differences in grain yield.

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