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Matter dynamics with respect to influences of C3 and C4 plants in four subtropical grasslands of Nilgiri Biosphere Reserve, the Western Ghats, India

  • Paulsamy, Subramanium;Kil, Bong-Soup;Sivashanmugam, Munusamy
    • Journal of Ecology and Environment
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    • 제35권4호
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    • pp.259-267
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    • 2012
  • Biomass changes, net primary production and transfer dynamics of dry matter in four subtropical humid montane grasslands at Ebbenadu, Korakundah, Thiashola and Wenlockdown in Nilgiri Biosphere Reserve (NBR), the Western Ghats, India, were investigated. Despite the early report of a higher density and dominance of $C_4$ species over $C_3$ species in these grasslands, no functional study of matter production had previously been carried out. Therefore, in order to determine the roles played by these two groups of species, the present study was attempted. The study revealed that the dry matter and net primary production contributed by $C_4$ species were much greater than those of its $C_3$ counterpart in all the grasslands. The turnover rate of aboveground dry matter for both $C_3$ and $C_4$ species was generally rapid, whereas it was slow for belowground parts, and litter components of $C_3$ and $C_4$ species together. In all grasslands, generally about 60% and 10% of the input were channeled to aboveground and belowground parts, respectively, by the $C_4$ species, whereas the $C_3$ species transferred only about 22% and 8% of dry matter to aboveground and belowground parts, respectively. The total disappearance of dry matter was 2.73, 2.10, 3.19, and $1.96\;g\;m^{-2}\;day^{-1}$ in Ebbenadu, Korakundah, Thiashola and Wenlockdown grasslands, which was 48.83%, 44.30%, 54.81%, and 41.09% of the total input, respectively, in these grasslands. This resulted in a considerable surplus dry matter production in all the grasslands studied. This balance sheet of dry matter in community function indicates that all the four studied grasslands were supporting the existing wild animals adequately in terms of pasture supply. The study further revealed that the stronger establishment of $C_4$ species rather than their $C_3$ counterparts, in terms of higher density and dominance, has driven them to play major roles in matter dynamics and system transfer functions in all the four grasslands studied. This fact evidenced that pasture from $C_4$ species for wild animals was determined to be higher in comparison to that of $C_3$ species.

돼지에 있어서 양적 형질 유전자좌(QTL) 발현 특성 분석을 위한 통계적 검정 모형 설정 (Designing of the Statistical Models for Imprinting Patterns of Quantitative Traits Loci (QTL) in Swine)

  • 윤두학;공홍식;조용민;이지웅;최익서;이학교;전광주;오성종;정일정
    • 한국수정란이식학회지
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    • 제19권3호
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    • pp.291-299
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    • 2004
  • 요크셔종과 버크셔종 교배 실험 집단을 활용하여 양적형질 유전자좌 (QTL)의 발현 특성 관련 유전 양식을 조사하였다. 총 512두의 F$_2$ 자손이 F$_1$간의 65교배 조합으로부터 생산되었으며 표현형 조사 기록은 일당증제량(ADG), 평균 등지방 두께(ABF), 10번째 등뼈 부위 등지방 두께(TRF) 및 등심단면적(LEA), 최후 척추부위 등지방 두께 (LRF)였다. 125종의 유전자 표지 (microsatellite)에 대한 3세대 개체별 유전자형이 분석되었으며 이들 정보를 통하여 최소자승 회귀 모형을 이용한 interval mapping 방법을 적용하였다. QTL의 유전양식 여부 검정에 대한 절차를 도식화하기 위해 귀무가설인 통상의 벤델리안 모형에 근거를 두고 수행하였다. 경험적 다중 검정 통계량에 대한 임계치는 단일 개개의 염색체 수준과 게놈 전반에 걸친 실험수준으로 유도하였으며, permutation에 의해 유도된 임계치의 유효성을 검증하기 위해 본 연구에 활용된 실험축 집단 구조와 유사한 simulation 집단 구조에 의해 산출된 결과들과 비교하여 유효성이 인정되었다. 본 연구에 활용된 실험축 집단구조와 Genome 전반에 걸친 QTL imprinting 여부를 조사한 결과 13종의 QTL 에 대한 imprinting이 확인되었으며 이들 중 9종의 QTL 유전 양식은 부계로부터 전달된 자손에게만 발현되는 것으로 추론되었다.

cDNA Cloning and Polymorphism of the Porcine Carbonic Anhydrase III (CA3) Gene

  • Wu, J.;Deng, Changyan;Xiong, Y.Z.;Zhou, D.H.;Lei, M.G.;Zuo, B.;Li, F.E.;Wang, J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권3호
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    • pp.324-328
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    • 2006
  • Carbonic anhydrase III (CA3) is a member of a multigene family that encode carbonic anhydrase isozymes. In this study, a complete coding sequence of the pig CA3 gene which encodes a 260 amino-acid protein was determined. The amino acid comparison showed high sequence similarities with previously identified human (86.5%) CA3 gene and mouse (91.5%) Car3 gene. The partial genomic DNA sequences were also investigated. The length of intron 1 was 727 bp. Comparative sequencing of three pig breeds revealed that there was a T${\rightarrow}$C substitution at position 363 within intron 1. The substitution was situated within a NcoI recognition site and was developed as a PCR-restriction fragment length polymorphism (RFLP) marker for further use in population variation investigations and association analysis. Two alleles (A and B) were identified, and 617 bp fragments were observed for the AA genotype and 236 bp and 381 bp fragments for the BB genotype. The polymorphism of CA3 was detected in 8 pig breeds. Allele B was predominant in the Western pig breeds. In addition, association studies of the CA3 polymorphism with carcass traits in 140 $Yorkshire{\times}Meishan$ $F_2$ offspring showed that the NcoI PCR- RFLP genotype may be associated with variation in several carcass traits of interest for pig breeding. Allele B was associated with increases in lean meat percentage, loin eye height and loin eye area. Statistically significant association with backfat thickness was also found; pigs with the AB genotype had much less backfat thickness than AA or BB genotypes.

Construction Claims Prediction and Decision Awareness Framework using Artificial Neural Networks and Backward Optimization

  • Hosny, Ossama A.;Elbarkouky, Mohamed M.G.;Elhakeem, Ahmed
    • Journal of Construction Engineering and Project Management
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    • 제5권1호
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    • pp.11-19
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    • 2015
  • This paper presents optimized artificial neural networks (ANNs) claims prediction and decision awareness framework that guides owner organizations in their pre-bid construction project decisions to minimize claims. The framework is composed of two genetic optimization ANNs models: a Claims Impact Prediction Model (CIPM), and a Decision Awareness Model (DAM). The CIPM is composed of three separate ANNs that predict the cost and time impacts of the possible claims that may arise in a project. The models also predict the expected types of relationship between the owner and the contractor based on their behavioral and technical decisions during the bidding phase of the project. The framework is implemented using actual data from international projects in the Middle East and Egypt (projects owned by either public or private local organizations who hired international prime contractors to deliver the projects). Literature review, interviews with pertinent experts in the Middle East, and lessons learned from several international construction projects in Egypt determined the input decision variables of the CIPM. The ANNs training, which has been implemented in a spreadsheet environment, was optimized using genetic algorithm (GA). Different weights were assigned as variables to the different layers of each ANN and the total square error was used as the objective function to be minimized. Data was collected from thirty-two international construction projects in order to train and test the ANNs of the CIPM, which predicted cost overruns, schedule delays, and relationships between contracting parties. A genetic optimization backward analysis technique was then applied to develop the Decision Awareness Model (DAM). The DAM combined the three artificial neural networks of the CIPM to assist project owners in setting optimum values for their behavioral and technical decision variables. It implements an intelligent user-friendly input interface which helps project owners in visualizing the impact of their decisions on the project's total cost, original duration, and expected owner-contractor relationship. The framework presents a unique and transparent hybrid genetic algorithm-ANNs training and testing method. It has been implemented in a spreadsheet environment using MS Excel$^{(R)}$ and EVOLVERTM V.5.5. It provides projects' owners of a decision-support tool that raises their awareness regarding their pre-bid decisions for a construction project.

자연과 수업에 증거집(포트폴리오) 평가의 적용이 초등학교 학생들의 과학 지식, 탐구능력 및 태도에 미치는 영향 (The Effects of Portfolio Assessment on Elementary School Students' Science Knowledge, Inquiry Ability and Science Attitudes)

  • 김혜정;김찬종
    • 한국과학교육학회지
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    • 제19권1호
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    • pp.19-28
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    • 1999
  • 이 연구의 주요 목적은 증거집(포트폴리오) 평가를 적용할 때 초등학교 학생들의 과학 지식, 탐구능력, 태도에 미치는 영향을 알아보고 학생들의 증거집 평가에 대한 인식을 조사하는 것이다. 비교집단은 충북 청원군 미원면의 M초등학교 4학년 45명이며, 실험집단은 대전광역시 G초등학교 4학년 36명이다. 사전 검사로 과학지식 검사1, 과학탐구능력검사, 과학 태도 검사를 두 집단에 실시하였다. 실험집단은 증거집 평가를 적용한 수업을 비교집단은 전통적인 수업을 약 6주 동안 실시하였다. 두 집단 모두 사후 검사로 과학지식 검사2, 과학 탐구능력검사, 과학 태도 검사를 실시하였다. 증거집 평가에 대한 인식 검사지를 실험집단에 실시하였다. 결과는 SPSS를 이용하여 통계적으로 분석하였다. 비교집단이 실험집단보다 과학 지식 검사에서 높은 성취를 보였다. 과학 탐구능력과 과학 태도에서는 의미있는 차이가 없었다. 하위 영역 분석 결과 과학적 태도에서 실험집단이 비교집단보다 통계적으로 유의미하게 높은 성취를 보였다. 학생들의 증거집 평가에 대한 인식은 매우 긍정적으로 나타났다. 학생들은 증거집 평가에 대한 흥미, 학습 참여도, 자기 주도적 학습, 고차적 사고, 앞으로 참여 의사 등과 같은 대부분의 하위 영역에서 긍정적인 반응을 보였다.

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수종토양중(數種土壤中)에서 3, 4-DCA 및 TCAB의 변화(變化)에 관(關)한 연구(硏究)제1보(第一報)-$^{14}C$-환표식(換標識) 및 비표식(非標識) TCAB의 신합성방법(新合成方法)- (A Study on the Evolution of 3, 4-DCA and TCAB in Some Selected Soils [Part I]-A New Method of Synthesizing $^{14}C-ring-labeled$ and Non labeled TCAB-)

  • 이재구;제씨포로니엘;지카트로씨
    • Applied Biological Chemistry
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    • 제20권1호
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    • pp.109-116
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    • 1977
  • Phenyl carbamates, phenyl ureas 및 acylanilides등의 제초제(除草劑)는 토양중(土壤中)에서 미생물(微生物)의 작용(作用)에 의(依)하여 발암성(發癌性) 물질(物質)로 알려진 Azo 화합물(化合物)을 형성(形成)하여 많은 주목(注目)을 끌고있다. 따라서 많은 연구자(硏究者)들은 이의 연구(硏究)에 있어 azo화합물(化合物)의 일종(一種)인 TCAB를 3,-dichloro nitrobenzene으로부터 합성(合性)하였으나 탈염소화(脫鹽素化) 작용(作用) 등(等)의 결점(缺點) 있고 또한 본(本) 연구(硏究)에서는 환표식(環標識) $^{14}C-3,4-DCA$로부터 환표식(環標識) $^{14}C-TCAB$를 합성(合成)할 필요(必要)가 있었으므로 본방법(本方法)을 고찰(考案)하였다. 즉(卽) 3,4-DCA를 Pyridine에 녹인후 공기(空氣) 존재하(存在下)에서 $60^{\circ}C$로 5-12 시간(時間) 동안 CuCl과 반응(反應)시켜 80.2% 수율(收率)로 표식(標識) 및 비표식(標識) TCAB를 합성(合成) 하였으며 그의 정제과정(精製過程)을 상세(詳細)히 기술(記述)하였다. 또한 TCAB 이성체(異性體)의 성질(性質)을 Autoradiography, TLC, GLC, IR 및 M.S로 검토(檢討) 확인(確認)하였다.

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GIS를 이용한 도심지 Nonpoint Source 오염 물질의 평가연구 (Urban Nonpoint Source Pollution Assessment Using A Geographical Information System)

  • 김계현
    • Spatial Information Research
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    • 제1권1호
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    • pp.39-53
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    • 1993
  • 지리 정보시스템(Geographical Information System)을 이용하여 도심지 Non Pount Source오염 물질의 양이 오염원 종류별로 확인되고 적절하한 오염감소를 위한 대책이 마련되었다. 경험에 의한 공해물질 예측모델을 운용하기 위한 모든 입력 자료들이 도심지의 거리 구획별(Street block)별로 제공되어 각 거리 구획별 오염량이 계산되었다. 계산된 오염량은 각 우수 배출구별로 합산되어 오염량이 많은 지역이 판명되었다. 또한 오염량을 줄이기 위하여 인공호수를 만들기 위한 적지분석이 수행되었으며, 그에 따른 비용분석이 이루어졌다. 본 연구는 지형정보시스템의 도심지 공해연구에의 기여도를 입증시켜 주었다.

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공항 지상 근무자의 심폐소생술 수행능력 (Performance Ability after CPR Education of the ground workers in an airport)

  • 신지훈
    • 한국응급구조학회지
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    • 제13권3호
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    • pp.29-40
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    • 2009
  • Objective : This study is an experimental study which is designed to examine the differences between knowledge and self-confidence before and after theory education(CPR PPT material) based on guidelines of CPR and emergency cardiac treatment of American Heart Association(AHA, 2005) and video self-instruction program for the general public by Korean Association of Cardiopulmonary Resuscitation(KACPR), trace CPR performance ability after CPR and AED education and investigate the accuracy of artificial respiration and chest compression, and know the difference in CPR performance abilities including AED. Methods : Subjects of this study include ground crews and staffs at M airport in G province equipped with emergency equipments for CPR according to Art. 47, Sec. 2 of Emergency Medical Law, airport police, rent-a-cops, security guard, quarantine officer, custom officer, and communication, electricity, civil engineering, facility management staff, airport fire fighting staff, air mechanic, traffic controller, and airport management team among airport facility management staffs. They were given explanation of necessity of research and 147 of 220 subjects who gave consent to this research but 73 who were absent from survey were excluded were used as subjects of this study. of 147 subjects, there were 102 men and 45 women. Results : 1) Knowledge score of CPR was $6.18{\pm}0.87$ before instruction and it was increased to $15.12{\pm}1.78$ after instruction, and there was statistically significant difference. 2) Self-confidence score in CPR was $3.16{\pm}0.96$ before instruction and it was increased to $7.05{\pm}0.75$ after instruction, and there was statistically significant difference. 3) Total average score in CPR performance ability after instruction was 7.46 out of 9, performance ability was highest in confirmation of response as 144(97.95%), follwed by request of help as 140(95.25%) and confirmation of respiration as 135(91.83%), and lowest in performing artificial respiration twice(gross elevation of chest) as 97(65.98%). Accuracy of artificial respiration(%) was $28.60{\pm}16.88$ and that of chest compression(%) was $73.10{\pm}22.16$. 4) Performance ability of AED after instruction showed proper performance in power on by 141(95.91%) and attaching pad by 135(91.83%), hand-off for analyzing rhythm showed 'accuracy' in 115(78.23%) and 'non-performance' in 32(21.77%), delivery of shock and hand-off confirmation showed 'accuracy' in 109(74.14%) and 'inaccuracy' in 38(25.86%), and beginning chest compression immediately after AED was done by 105(71.42%).

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Dietary phosphorus deficiency impaired growth, intestinal digestion and absorption function of meat ducks

  • Xu, Huimin;Dai, Shujun;Zhang, Keying;Ding, Xuemei;Bai, Shiping;Wang, Jianping;Peng, Huanwei;Zeng, Qiufeng
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권12호
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    • pp.1897-1906
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    • 2019
  • Objective: An experiment was conducted to investigate the effects of dietary non-phytate phosphorus (nPP) deficiency on intestinal pH value, digestive enzyme activity, morphology, nutrient utilization, and gene expression of NaPi-IIb in meat ducks from 1 to 21 d of age. Methods: A total of 525 one-d-old Cherry Valley ducklings were fed diets (with 7 pens of 15 ducklings, or 105 total ducklings, on each diet) with five levels of nPP (0.22%, 0.34%, 0.40%, 0.46%, or 0.58%) for 21 d in a completely randomized design. Five experimental diets contained a constant calcium (Ca) content of approximately 0.9%. Body weight (BW), body weight gain (BWG), feed intake (FI), and feed to gain ratio (F:G) were measured at 14 and 21 d of age. Ducks were sampled for duodenum and jejunum digestion and absorption function on 14 and 21 d. Nutrient utilization was assessed using 25- to 27-d-old ducks. Results: The results showed ducks fed 0.22% nPP had lower (p<0.05) growth performance and nutrient utilization and higher (p<0.05) serum Ca content and alkaline phosphatase (ALP) activity. When dietary nPP levels were increased, BW (d 14 and 21), BWG and FI (all intervals), and the serum phosphorus (P) content linearly and quadratically increased (p<0.05); and the jejunal pH value (d 14), duodenal muscle layer thickness (d 14), excreta dry matter, crude protein, energy, Ca and total P utilization linearly increased (p<0.05); however, the serum ALP activity, jejunal $Na^+-K^+$-ATPase activity, and duodenal NaPi-IIb mRNA level (d 21) linearly decreased (p<0.05). Conclusion: The results indicated that ducks aged from 1 to 21 d fed diets with 0.22% nPP had poor growth performance related to poor intestinal digestion and absorption ability; but when fed diets with 0.40%, 0.46%, and 0.58% nPP, ducks presented a better growth performance, intestinal digestion and absorption function.

Skin Protection Seminars to Prevent Occupational Skin Diseases: Results of a Prospective Longitudinal Study in Apprentices of High-risk Professions

  • Wilke, Annika;Brans, Richard;Nordheider, Kathrin;Braumann, Antje;Hubner, Anja;Sonsmann, Flora K.;John, Swen M.;Wulfhorst, Britta
    • Safety and Health at Work
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    • 제9권4호
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    • pp.398-407
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    • 2018
  • Background: Occupational skin diseases (OSDs) are frequent in professions with exposure to skin hazards. Thus, a health educational intervention for apprentices of high-risk professions was conducted. It was the aim of this study to gain insight into possible effects of this intervention. Methods: A one-time skin protection seminar was conducted in 140 apprentices of health-related and non-health-related professions [trained cohort (TC)]. In addition, 134 apprentices of the same occupations were monitored [untrained cohort (UTC)]. The OSD-specific knowledge and the skin condition of the hands were assessed at baseline (T0), after the seminar (T1), and after 6 (T2) and 12 months (T3). Results: The OSD-specific knowledge increased in all cohorts from T0 to T3, but we found a significantly higher knowledge in the TC at T2 (p < 0.001, t = 3.6, df = 196, 95% confidence interval = 0.9, 3.3) and T3 (p < 0.001, t = 3.8, df = 196, 95% confidence interval = 1.0, 3.2) compared to the UTC. Our results indicated a better skin condition of the hands in the TC of the health-related professions but not in the non-health-related professions. Conclusion: The study indicates that an educational intervention may positively influence the disease-specific knowledge and the prevalence of OSD in apprentices. However, definite conclusions cannot be drawn because of the heterogeneous study cohorts and the study design. Future research should aim at tailoring primary prevention to specific target groups, e.g., in view of the duration and frequency of skin protection education, different professions, and gender-specific prevention approaches.