• 대한의생명과학회지
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• 제15권2호
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• pp.147-152
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• 2009

Lycopus lucid us Turcz is well known as traditional Chinese medicine, and it has been shown to exhibit antiinflammatory, -allergic and -oxidative effect. However, its anti-cancer properties have not been examined yet. In this study, we investigated the effect of the methanol extract of Lycopus lucid us Turcz on anti-cancer effect in MCF-7 human breast cancer cells. Treatment of Lycopus lucidus Turcz extract induced apoptosis and inhibition of cell proliferation in dose- and time-dependent manner. Apoptosis in the MCF-7 cells was characterized with the changes in nuclear morphology; decrease of Bcl-2 and caspase-7 expression; and increase of cleaved poly ADP-ribose polymerase(PARP). Furthermore, treatment of Lycopus lucidus Turcz extract caused the down-regulation of cell cycle-related protein including, cdk4, cyclin D1 and E2F-1. These results suggest that Lycopus lucidus Turcz might have the therapeutic value against human breast cancer cells.

• 식물분류학회지
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• 제43권2호
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• pp.99-102
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• 2013

큰쉽싸리 (Lycopus lucidus Trucz. ex Benth. var. hirtus Regel) 자연집단의 자생지가 강원도 지역에서 발견되었다. 큰쉽싸리는 잎과 줄기에 많은 털을 갖는 특징으로 쉽싸리(L. lucidus var. lucidus)와 구분되는 것으로 알려져 있으나, 본 본류군의 분류학적 한계는 연구 보고된 바가 없고, 본 분류군의 한반도 분포 역시 명확히 알려진 바가 없다. 따라서, 본 논문에서는 본 분류군의 상세한 형태학적 기재와 지리학적 분포 및 습성을 제시하였다.

• 한국의류학회지
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• 제36권3호
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• pp.324-334
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• 2012

In this research, the dyeing properties of $Lycopus$ $lucidus$ Turcz on cotton and silk fabrics were evaluated to establish a scientific database of natural dyes. The optimum dyeing conditions were identified with K/S values depending on the dyeing concentration, temperature, time, and pH. For the optimum conditions, the color changes for different synthetic mordants were observed as L, $a^*$, $b^*$ and H, V, C. The color fastness after dyeing and mordanting was also examined. Additionally, the antibiosis of $Lycopus$ $lucidus$ Turcz was examined. The results were as follows: For the cotton, the optimized dyeing conditions were 300% (o.w.f.), $50^{\circ}C$, 40 min., and pH 5. For the silk fabric, the conditions were 450% (o.w.f.), $70^{\circ}C$, 60 min., and pH 4. The K/S value of dyed cotton improved about 1.03-2.78 folds after mordanting. The fabric color was yellow in the absence of mordanting, while it was reddish yellow after post-mordanting with FFC. Although the color changed with the mordanting method and mordant, the overall hue was yellow. Thus, $Lycopus$ $lucidus$ Turcz appears to have the potential for use as a natural dyeing material for a yellow hue. The K/S value of the dyed silk fabric improved about 1.05-1.40 folds after mordanting. The fabric showed a reddish yellow color when pre-mordanted with Al and Cu. The washing and dry-cleaning fastness of the cotton and silk fabrics were at the 4-5 level. The light fastness of the cotton improved after mordanting; however, pre-mordanting was more effective than post-mordanting or the absence of mordanting. The dyed cotton, silk fabrics showed excellent antibiosis.

• 대한본초학회지
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• 제31권6호
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• pp.21-28
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• 2016

Objectives : This study was performed to investigate the antioxidant activity of water extracts from Lycopus lucidus Turcz. ex Benth. leaves, stems and roots at the $100{\mu}g/m{\ell}$ concentration. Methods : The different part of Lycopus lucidus Turcz. ex Benth. extract was prepared using water. The antioxidant activities of polyphenol contents, total flavonoid contents, DPPH(1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity, SOD like activity, hydroxyl radical, ABTS(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), $Fe^{2+}$ chelating, and nitrite scavenging activity. Results : The total polyphenol and total flavonoid content of leaves were the highest at $221.85{\mu}g/mg$ and $794.13{\mu}g/mg$, respectively. Electron donating ability was the 79.68% in the water extract from leaves. The ABTS radical scavenging activity of the hot extracts, leaves ${\gg}$ roots > stems was higher in the order. It was shown the highest at 94.53% in the water extract from leaves, which showed a value equal to 94.7% of ascorbic acid. The hydroxyl radical scavenging activity was the highest at 8.07% in the water extract from leaves. SOD like activity and $Fe^{2+}$chelating activity were leaves of 12.3% and 27%, respectively, which were much higher than those of any other parts. The nitrite scavenging ability of extracts was increased at pH 2.5, and those was the highest in leaves of 83.03%. Its were more than twice the 41.61% of BHT. Conclusion : The results suggest that Lycopus lucidus Turcz. ex Benth. can be used as nutraceutical foods and natural antioxidant.

• 한국식품영양학회지
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• 제32권3호
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• pp.208-215
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• 2019

In this paper, we investigate to determine quality characteristics, fatty acid composition and cytotoxic effect of extracts and fractions from whole Lycopus lucidus Turcz. roots. Additionally, we evaluated cytotoxic activity against the growth of human fibrosarcoma cells (HT-1080) and human gastric adenocarcinoma (AGS), human colon cancer cell (HT-29) lines using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Acetone+methylene chloride (A+M) and methanol (MeOH) extracts from L. lucidus Turcz. were obtained through solvent extraction. Then we further fractionated both extracts with n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water. In fatty acid composition, L. lucidus Turcz. contained 33.2% of 18:1n-9 and 1.81% of 18:3n-3, respectively. The incorporation of treatment with A+M and MeOH extracts and n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water fractions dose-dependently increased cytotoxicity against the growth of HT-1080 and AGS, HT-29 cancer cells (p<0.05). The A+M extract had a higher inhibitory effect on the growth of all cancer cells in comparison to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions showed a higher inhibitory effect after proliferating the three cancer cells. These results suggest that the 85% aq. MeOH and n-hexane fractions have a potential to inhibit the growth of human cancer cell lines.

• Journal of Plant Biotechnology
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• 제45권2호
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• pp.131-139
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• 2018

본 연구에서는 쉽싸리의 재분화와 순화에 영향을 주는 탄소급원의 효과에 관한 연구를 수행하였다. 식물체 재분화는 1/2MS 배지에 3 ~ 10%의 sucrose와 glucose를 각각 다른 농도로 처리하여 재분화를 유도하였다. 그 결과 3%와 5% sucrose 처리에서 줄기증식과 발근이 증가되었으며, 고농도 처리에서는 줄기와 뿌리 길이 등의 생육이 억제되었다. 기외 순화 중 3% 혹은 5% sucrose 처리 배지에서 생육한 식물체의 뿌리비대, 뿌리길이, 줄기 길이 및 생존율은 glucose 및 다른 농도처리와 비교하여 가장 높게 나타났다. 따라서 3% 및 5% sucrose 처리는 쉽싸리의 기내 생육과 순화를 위해 가장 적정한 농도로 확인되었다.

• 약학회지
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• 제46권6호
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• pp.405-410
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• 2002

The effect of aqueous extract of Lycopus lucidus Turcz. (Labiatae)(LLAE) on mast cell-mediated immediate-type allergic reactions was investigated. LLAE (0.01 to 1 mg/g) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80. LLAE (0.001 to 1 mg/g) also dose-dependently inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE. When LLAE was pretreated at the same concentration with systemic anaphylaxis, serum histamine levels were reduced in a dose-dependent manner. LLAE (0.001 to 1 mg/mι) dose-dependently inhibited histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80. The level of cAMP in human mast cell line (HMC-1) cells, when LLAE (1 mg/mι) was added, significantly was increased, compared with that of normal control. These results provide evidences that LLAE may be beneficial in the treatment of allergic diseases.

• 생약학회지
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• 제23권1호
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• pp.29-33
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• 1992

The essential oils of Lycopus lucidus have been studied. The essential oil obtained from the dried herba by steam distillation followed by fractionation by column chromatography was analysed by gas chromatography-mass spectrometry(GC-MS). The compounds identified by GC-MS were carvacrol, 2,5-dimethoxy-p-cymene, trans-caryophyllene, spathurenol and $trans-{\beta}-farnesene$. Two compounds, thymol and caryophyllene were isolated by silica gel column chromatography and analyzed by TLC, IR, Mass and NMR. And the weak diuretic effects of essential oil and water extract from the dried drugs were observed in rats.

• 생명과학회지
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• 제28권7호
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• pp.841-848
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• 2018

초석잠 acetone+methylene chloride (A+M) 및 methanol (MeOH) 추출물들의 총 플라보노이드 함량은 $57.05{\pm}1.08$ 및 $37.42{\pm}0.12mg/g$으로 A+M 추출물의 총 플라보노이드 함량이 높았다. 택란 A+M 및 MeOH 추출물들의 총 플라보노이드 함량은 $233.22{\pm}2.60$ 및 $46.31{\pm}0.54mg/g$으로 A+M 추출물의 총 플라보노이드 함량이 높았다 이에 따라 총 플라보노이드 함량은 A+M 추출물에서 높게 나타남을 알 수 있었다. 각 용매별 추출물들의 1,1-Diphenyl-2-picryhydrazyl (DPPH) 라디칼 소거활성을 각각 0.05, 0.1, 0.25 및 0.5 mg/ml의 농도로 대조군 [L-ascorbic acid, Butylated hydroxyl toluene (BHT)]과 비교하였다. 먼저 추출물들과 비교했을 때 택란 A+M 추출물은 다른 추출물과 비교했을 때 활성산소 소거능이 우수하였다. 이는 앞서 택란 A+M 추출물의 높은 총 플라보노이드 및 총 페놀 함량과 연관성이 있는 것으로 여겨진다. 2.2'-Azino-bis (3-ethylbenothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS+) 라디칼 소거활성에서도 택란 A+M추출물과 초석잠 A+M추출물은 합성 항산화제인 BHT와 비교하여 0.25 및 0.5 mg/ml 농도에서 BHT 경우, 93.5 및 95.3%이며, 초석잠 A+M추출물은 89.0 및 89.8%의 소거능을 나타내었으며 택란 A+M추출물은 각각90.2% 및 88.0%로 대조군과 유사한 값을 나타내었다. 초석잠 및 택란 A+M 및 MeOH 추출물을 0.05 및 0.025 mg/ml의 농도로 인체 섬유육종세포(HT-1080)에 처리하여 세포 내 활성 산소종을 측정한 결과 두 추출물들 모두 측정시간 120분이 지남에 따라 높은 세포 내 활성산소종 억제효과를 나타내었다. A+M 추출물의 경우 MeOH 추출물과 비교 하였을 때 세포 내 활성산소종을 상대적으로 크게 억제하였으며 특히 택란 A+M 추출물 0.05 mg/ml 농도에서는 대조군과 비교하여 높은 억제효과를 나타내었다. 따라서 본 연구 결과는 택란 A+M 추출물에 의한 항산화 효과가 우수하였고 이는 택란 초석잠 A+M 추출물의 높은 함량의 플라보노이드와도 연관성이 있음을 나타낸다.

• 생명과학회지
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• 제30권2호
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• pp.147-155
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• 2020

택란 잎 A+M 및 MeOH 추출물의 총 플라보노이드 함량은 각각 55.7±2.04 및 119.6±7.78 mg/g으로 택란 잎 MeOH 추출물의 총 플라보노이드 함량이 높았고 초석잠 잎 추출물들보다 높았다. 4종 추출물들과 비교했을 때 택란 A+M 추출물은 다른 추출물과 비교했을 때 DPPH 소거능이 우수하였다. ABTS 라디칼 소거활성에서도 초석잠 및 택란 잎 MeOH 추출물은 0.5 mg/ml 농도에서 각각 85 및 91%의 라디칼 소거능을 나타내었으며 합성항산화제인 BHT와 L-ascorbic acid와 유사한 소거활성을 나타내었다. 이러한 항산화 활성은 앞서 택란 잎 MeOH 추출물의 높은 총 플라보노이드 함량과 연관성이 있는 것으로 여겨진다. 초석잠 및 택란 잎 A+M 및 MeOH 추출물을 0.05 및 0.025 mg/ml의 농도로 인체 섬유육종세포(HT-1080)에 처리하여 세포 내 활성산소종을 측정한 결과 두 추출물들 모두 측정시간 120분이 지남에 따라 높은 세포 내 활성산소종 생성 억제효과를 나타내었다. 초석잠 잎의 경우 A+M 추출물에 의한 활성산소종 억제효과가 높았고 반면 택란 잎 MeOH 추출물은 0.05 mg/ml 농도에서 대조군과 비교하여 높은 억제효과를 나타내었다. 인체 암세포들(AGS, HT-29 및 HT-10180)에 대한 세포독성 효과를 측정한 결과 4종 추출물들 중 택란 잎 A+M 추출물에 의한 세포독성 효과가 가장 높았다. 본 연구 결과들로부터 라디칼 소거 활성에서 택란 잎 MeOH 추출물에 의한 항산화 효과가 높았으며 암세포에 대한 세포독성 효과는 택란 잎 A+M 추출물에서 가장 높은 활성을 확인하였다. 따라서 이는 택란 잎 A+M 및 MeOH 추출물들에 함유된 높은 함량의 총 플라보노이드와도 연관성이 있음을 나타낸다.