• Title/Summary/Keyword: Lycopus

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Induction of G1 Arrest by Methanol Extract of Lycopus lucidus in Human Lung Adenocarcinoma A549 Cells (택란 메탄올 추출물에 의한 인체 폐암 세포주 A549의 G1 arrest 유발)

  • Park, Hyun-Jin;Jin, Soojung;Oh, You Na;Yun, Seung-Geun;Lee, Ji-Young;Kwon, Hyun Ju;Kim, Byung Woo
    • Journal of Life Science
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    • v.23 no.9
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    • pp.1109-1117
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    • 2013
  • Induction of G1 Arrest by Methanol Extract of Lycopus lucidus in Human Lung Adenocarcinoma A549 Cells Lycopus lucidus, a herbaceous perennial, is used as a traditional remedy in East Asia, including China and Korea. It has been reported that L. lucidus has anti-allergic effects, inhibitory effects on cholesterol acyltransferase in high glucose-induced vascular inflammation, and anti-proliferative effects in human breast cancer cells. However, the molecular mechanisms of the anti-cancer effects of L. lucidus have not yet been fully determined. In this study, we evaluated the anti-cancer effect and the mechanism of action of L. lucidus in human lung adenocarcinoma A549 cells using methanol extracts of L. lucidus (MELL). MELL treatment showed cytotoxic activity in a dose-dependent manner and induced G1 arrest in A549 cells. The induction of G1 arrest by MELL was associated with the up-regulation of phospho-CHK2 and the down-regulation of Cdc25A phosphatase. In addition, MELL treatment induced decreased expression of G1/S transition-related proteins, including CDK2, CDK4, CDK6, cyclin D1 and cyclin E. MELL also regulated the mRNA expression of CDK2 and cyclin E. On the other hand, the expression of p53 and the cyclin-dependent kinase inhibitor p21 was not induced by MELL. Collectively, these results suggest that MELL may exert an anti-cancer effect by cell cycle arrest at G1 phase through the ATM/CHK2/Cdc25A/CDK2 pathway in A549 cells.

Comparison of Flavonoid Content and Antioxidant Effect of Extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz (초석잠 및 택란 추출물의 플라보노이드 함량 및 항산화 활성 비교)

  • Lee, Jung Woo;Lim, Sun Young
    • Journal of Life Science
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    • v.28 no.7
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    • pp.841-848
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    • 2018
  • The flavonoid content and antioxidant effects of extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz were compared. The flavonoid content of the acetone + methylene chloride (A+M) extract of L. lucidus Turcz was 233.2 mg/g, suggesting that the extract was greater than that of S. sieboldii Miq. In the DPPH assay and the A+M and methanol (MeOH) extracts from L. lucidus Turcz had greater scavenging effects than those of S. sieboldii Miq. (p<0.05). The A+M extract from L. lucidus Turcz (0.5 mg/ml concentration) had an 82% scavenging effect in the DPPH assay. In the ABTS assay, A+M extracts from both S. sieboldii Miq. and L. lucidus Turcz (0.5 mg/ml concentration) had scavenging effects of 90% and 88%, respectively (p<0.05), suggesting that both A+M extracts had greater scavenging effects than those of both MeOH extracts. In a 120 min ROS production assay, all tested extracts dose-dependently decreased the cellular ROS production that was induced by $H_2O_2$, as compared to those produced by exposure to the extract-free control. The A+M extracts from both S. sieboldii Miq. and L. lucidus Turcz had greater inhibitory effects on cellular ROS production than those of both MeOH extracts at all concentrations tested. Treatment with the A+M extracts from S. sieboldii Miq. and L. lucidus Turcz (0.25 mg/ml concentration) inhibited the cellular ROS production by 60% and 86%, respectively. These results suggest that the A+M extracts of Stachys sieboldii Miq. and L. lucidus Turcz inhibit cellular oxidation and may contain valuable bioactive compounds, such as flavonoids.

Studies on the Composition of Fatty Acid in the Lipid Classes of Seed Oils of the Labiatae Family (순형과(脣形科) 종실유(種實油)의 지질분획별(脂質分劃別) 지방산(脂肪酸) 조성(組成)에 관한 연구(硏究))

  • Joh, Yong-Goe;Lee, Ok-Kyoung;Lim, Young-Ju
    • Journal of the Korean Applied Science and Technology
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    • v.5 no.1
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    • pp.13-23
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    • 1988
  • Contents of total lipids, neutral lipids, glycolipids and phospholipids of seed oils of 16 species of the Labiatae family were determined and their fatty acid compositions were analyzed by gas-liquid chromatography. The results were summarized as follows. 1) Lipid contents of seeds were shown to be 40.6% in Perilla frutescens Britton var. japonica, 32.2% in P. frutescens britton var. acuta, 31.9% in lsodon japonicus, 32.7% in l. inflexus, 48.3% in l. serra, 35.1% in Mosls dianthera, 38.2% in M. punctulata, 33.4% in Nepeta cataria, 26.3% in Agastache rugosa, 30.9% in Eisholtzia ciliata, 18.9% in Salvia splendens, 23.9% in Lycopus maackianus, 49.5% in Clinopodium chinense var. parviflorum, 30.9% in Ametystea caerulea, 33.1% in Leonurus sibircus and 34.3% in Scutellaria basicalensis. 2) Contents of neutral lipids, glycolipids and phospholipids from the seed oils amounted to 98.6%, 0.7%, 0.8% in P. frutescens Britton var. japonica; 95.5%, 1.3%, 3.1% in P. frutescens Britton var. acuta; 95.1%, 1.8%, 3.1% in l. japoincus; 91.4%, 3.5%, 5.1% in l. inflexus; 96.8%, 0.7%, 2.5% in l, serra; 96.0%, 1.8%, 2.2% in Mosla dianthera; 94.7%, 2.0%, 3.3% in M. punctulata; 90.1%, 2.4%, 7.5% in Nepeta cataria; 90.1%, 3.4%, 6.5% in Agastache rugosa; 86.3%, 3.3%, 10.4% in Elsholtzia ciliata; 94.3%, 1.5%, 4.3% in Salvia splendens; 87.2%, 2.9%, 9.0% in Lycopus maackianus; 87.0%, 1.5%, 11.5% in Clinopodium chinense var. parviflorum; 91.8%, 1.6%, 6.6%; 95.5%, 0.4%, 4.1% in Leonurus sibricus; 89.0%, 1.4%, 9.6% in Scutellaria baicalensis. 3) Total lipids revealed the predominace of unsaturated fatty acids (82.0-94.5%) and larger variations were found in the composition of ${\alpha}-linolenic$ acid (0.4-67.9%) and linoleic acid (11.2-82.9%). High level of ${\alpha}-linoenic$ acid was present in P. frutescens Britton var. japonica (67.9%), P. frutescens Britton var, acuta (66.0%), lsodon japonicus (65.2%), l. inflexus (59.0%), l. serra (57.3%), Mosla dianthera (60.9%), Nepeta cataria (58.3%), Agastache rugosa (58.5%) and Elsholtzia ciliata (46.2%), and followed by linoleic acid (11.2-32.1%) and oleic acid (9.3-12.2%). However, linoleic acid was the most predominant component in the total lipids of Clinopodium chinense var. parviflorum (62.4%), Ametystea caerules (82.9%), Leonurus sibricus (60.9%) and Scutellaria baicalensis (63.4%), with very small amounts of ${\alpha}-linolenic$ acid (0.4-3.1%). The total lipids of Salvia splendens, Lycopus maackianus and Mosla punctulata also contained linoleic acid of 31.3%, 48.8% and 53.4%, with a considerable amount of ${\alpha}-linolenic$ acid of 34.5% 27.0% and 16.7%. Palmitic acid was the major saturated fatty acid in all the oils investigated (4.1-14.2%). 4) Fatty acid profiles of neutral lipids bore a close resemblance to those of total lipids in all the seed oils, but different from those of glycolipids and phospholipids. Fatty acid composition pattern of glycolipids and phospholipids showed a considerably increased level of saturated fatty acids (19.0-66.8%, 17.8-35.2%) mainly composed of palmitic acid and stearic acid, and a noticeable low level of unsaturated fatty acids (41.2-80.9%, 64.7-82.1%) which was ascribed to the decrease in ${\alpha}-linolenic$ acid of high ${\alpha}-linolenic$ acid seed oils, and in linoleic acid of high linoleic seed oils, compared to that of total lipids and neutral lipids.

Comparison of Antioxidant, Cytotoxicity and Flavonoid Content of Stachys sieboldii Miq. vs. Lycopus lucidus Turcz. Leaf Extracts (초석잠 및 택란 잎 추출물의 항산화 및 세포독성 활성과 총 플라보노이드 함량 비교)

  • Na, Eun;Lee, Jung Woo;Lim, Sun Young
    • Journal of Life Science
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    • v.30 no.2
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    • pp.147-155
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    • 2020
  • In this study, the antioxidant and cytotoxic effects and the flavonoid contents of leaf extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz. were compared. The flavonoid contents of the acetone + methylene chloride (A+M) and methanol (MeOH) extracts of L. lucidus Turcz. leaves were 55.7 and 233.2 mg/g, respectively. In a DPPH assay, A+M and MeOH extracts from L. lucidus Turcz leaves had a greater scavenging effect than those of S. sieboldii Miq. leaves (p<0.05). In an ABTS assay, MeOH extracts from S. sieboldii Miq. and L. lucidus Turcz (0.5 mg/ml concentration) leaves had scavenging effects of 85% and 91%, respectively (p<0.05), suggesting that both of the MeOH extracts had greater scavenging effects than both A+M extracts. In a 120 min ROS production assay, all tested extracts decreased the cellular ROS production induced by H2O2 compared to that produced by exposure to the extract-free control. The MeOH extract from L. lucidus Turcz leaves had a greater inhibitory effect on cellular ROS production (p<0.05). Treatment with A+M and MeOH extracts from both S. sieboldii Miq. and L. lucidus Turcz. leaves showed a dose-dependent increased cytotoxicity against the growth of AGS, HT-29 cancer cells, and HT-1080 (p<0.05). Both A+M extracts had a greater inhibitory effect on the growth of all cancer cells than both MeOH extracts. These results suggest that the MeOH extract of L. lucidus Turcz. leaves is effective in scavenging free radicals and inhibiting cellular oxidation, while the A+M extract inhibits proliferation of three types of cancer cell.

Free Radical Scavenging, Cytotoxic Effects, and Flavonoid Content of Fractions from Leaves of Lycopus lucidus Turcz. (택란 잎 추출물의 라디칼 소거활성과 세포독성효과 및 플라보노이드 함량)

  • Na, Eun;Lee, Jung Woo;Lim, Sun Young
    • Journal of Life Science
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    • v.29 no.3
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    • pp.337-344
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    • 2019
  • The free radical scavenging, cytotoxic effects, and flavonoid content of fractions from Lycopus lucidus Turcz leaves were here investigated. The flavonoid contents of 85% methanol (MeOH) and n-butanol (BuOH) fractions of the leaves were 41.5 mg/g and 77.2 mg/g, respectively. In DPPH and ABTs+ assays, 85% MeOH and n-BuOH fractions from the L. lucidus Turcz leaves had a greater scavenging effect (p<0.05). The n-BuOH fraction (0.5 mg/ml concentration) had scavenging effects of 88% and 92% in the DPPH and ABTs+ assays, respectively (p<0.05). Cell viability tests showed that treatment with L. lucidus Turcz leaf fractions caused cytotoxicity in the growth of AGS, HT-29, and HT-1080 cancer cells. Of the different fractions, the 85% MeOH sample displayed the highest cytotoxic activity; the $IC_{50}$ values of this fraction against AGS, HT-1080, and HT-29 cancer cells were 0.03 mg/ml, 0.14 mg/ml, and 0.16 mg/ml, respectively. These biological results indicate that the n-BuOH fraction was more effective in anti-oxidant activity while the 85% MeOH fraction was stronger in cytotoxic effects, and they suggest that these two fractions from L. lucidus Turcz leaves may contain valuable bioactive compounds, such as flavonoids.

Antioxidant, Antimicrobial and Cellular Protective Effects of Lycopus lucidus Extract and Fraction (택란 추출물 및 분획물의 항산화, 항균 및 세포 보호 효과)

  • Lee, Jin Kyoung;Park, Young Min;Lee, Sang Lae;Song, Ba Reum;Lee, Yun Ju;Park, Soo Nam
    • Applied Chemistry for Engineering
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    • v.30 no.1
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    • pp.114-121
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    • 2019
  • In this study, antioxidative, antibacterial and cytoprotective effects of the ethanol extract and ethylacetate fraction of Lycopus lucidus (L. lucidus) were compared and analyzed. Free radical scavenging activities ($FSC_{50}$) of the L. lucidus extract and fraction were found to be 65.1 and $64.9{\mu}g/mL$ respectively. In the $Fe^{3+}-EDTA/H_2O_2$ system, the reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) for the extract and fraction were 6.6 and $6.3{\mu}g/mL$, respectively which showed excellent total antioxidant abilities. The extract showed antibacterial activity against S. aureus, while the fraction showed in all the bacteria except for A. niger. The cytoprotective effect of L. lucidus extract was compared to that of the fraction and the effect against $^1O_2$-induced cellular damage of human erythrocytes (${\tau}_{50}$) was 51.3 and 73.7 min at $50{\mu}g/mL$, respectively. For the cytoprotective effect of keratinocytes damaged by $H_2O_2$ and UVB, the extracts did not show any efficacy but showed efficacy at $1-2{\mu}g/mL$, respectively. The fraction increased the cell viability up to 85.8 and 81.9%, respectively. As a result of intracellular ROS scavenging activity, the scavenging activity was observed at $1-2{\mu}g/mL$ of the fraction. From the results comparing the physiological activities of L. lucidus extract and the fraction, the ethylacetate fraction of L. lucidus has antioxidative effect similar to that of the extract whereas superior antimicrobial and cytoprotective effects than that of the extract. Overall, the ethylacetate fraction of L. lucidus protects cells from an external stress which can be used as a potential cosmetic material.

Neuroprotective Effects of Medicinal Herbs in the Transient Focal Ischemia

  • Oh, Jin-Kyung;Jung, Ji-Wook;Ahn, Nam-Yoon;Oh, Hye-Rim;Ryu, Jong-Hoon
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2003.11a
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    • pp.64-64
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    • 2003
  • It is thought that highly reactive oxygen radicals generated at the ischemia-reperfusion in case of strokes play an important role in damaging the brain. We examined the neuroprotective effects from the several medicinal herbs in the transient ischemic rat model and compared their effects with the free radical scavenging activities. Transient ischemia was induced by intraluminal occusion of the right middle cerebral artety for 120 min and reperfusion was continued for 22 h in rats. The free radical scavenging properties of medicinal herbs were examined in vitro by determination of the interaction with the 1,l-diphenyl-2-picrylhydrazyl (DPPH) stable free radical. Aqueous extracts of 11 medicinal herbs (200 mg/kg) were orally administered, promptly prior to reperfusion and 2 h after reperfusion. Total infarction volume in the ipsilateral hemisphere of ischemia reperfusion rats was significantly lowered by the treatment of 7 medicinal herbs (Sophora flavescens, Lycopus lucidus, Sanguisorba officinalis, Caesalpinia sappan, Albizia julibrissin, Rubia akane, Psoralea corylifolia, Prunella vulgaris). However, all of these medicinal herbs did not show antioxidative activities. These results suggest that neuroprotective effects of several drugs are not always correlated with their antioxidative properties.

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Isolation, Identification, and Activity of Rosmarinic Acid, a Potent Antioxidant Extracted from Korean Agastache rugosa (한국산 방아잎(배초향, Agastache rugosa O. Kuntze)에서 항산화물질 로즈마린산의 분리, 동정 및 활성)

  • Kim, Jong-Bum;Cho, Kang-Jin;Hwang, Young-Soo;Park, Ro-Dong;Kim, Jung-Bong
    • Applied Biological Chemistry
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    • v.42 no.3
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    • pp.262-266
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    • 1999
  • Thirty plants were screened for their antioxidative activity. The extracts of Agastache rugosa, Schizonepeta tenuifolia and Lycopus lucidus had high free radical(2,2-diphenyl-1-picrylhydrazyl) scavenging activities. Methanol extract of Agastache rugosa was fractionated with hexane, chloroform, ethyl acetate, and water. The ethyl acetate fraction showed the highest antioxidant activity in the DPPH test. The ethyl acetate fraction was applied to Sephadex LH-20 column, and the fractions showing antioxidative activity were collected and used for identification of the substance. The purified substance was applied to mass, IR, UV and NMR spectroscopy. The spectra of mass, IR, UV and NMR implied that the substance is a rosmarinic acids as a kind of phenolic compound. The rosmarinic acid has more antioxidative effect than those of BHT and ${\alpha}-tocopherol$ in the Rancimat test.

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Neuroprotective Effects of Medicinal Herbs in the Transient Focal Ischemia in Rats (일시적 국부 뇌허혈 동물모델에서 한약재의 신경보호 효과)

  • Oh, Jin-Kyung;Jung, Ji-Wook;Ahn, Nam-Yoon;Oh, Hye-Rim;Cheung, Jae-Hoon;Ryu, Jong-Hoon
    • Korean Journal of Pharmacognosy
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    • v.34 no.4 s.135
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    • pp.335-338
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    • 2003
  • It is thought that highly reactive oxygen species generated after strokes plays a key role in damaging the brain. We examined free radical scavenging activity and neuroprotective effects of several medicinal herbs in a rat model of transient ischemia. Free radical scavenging property of medicinal herbs was examined in vitro using 1,1-diphenyl-2-picrylhydrazyl stable free radical. Transient ischemia was induced by intraluminal occlusion of the right middle cerebral artery for 120 min, followed by reperfusion for 22 hr in rats. Aqueous extracts of 8 medicinal herbs (200 mg/kg) were orally administered twice to transient ischemic rat prior to reperfusion and 2 hr after reperfusion. Total infarction volume in the hemisphere ipsilateral to the ischemia-reperfusion was significantly decreased in 7 groups treated with Sophora flavescens, Lycopus lucidus, Sanguisorba officinalis, Caesalpinia sappan, Albizia julibrissin, Rubia akane, Psoralea corylifolia, or Prunella vulgaris. However, neuroprotective effects of these medicinal herbs were not correlated with their antioxidative activities. These results suggest that these medicinal herbs exert neuroprotection via antioxidative as well as unknown mechanism.

Anti-Adipogenic Effects of Ethanol Extracts Prepared from Selected Medicinal Herbs in 3T3-L1 Cells

  • Park, Min-Jun;Song, Ji-Hye;Shon, Myung-Soo;Kim, Hae Ok;Kwon, O Jun;Roh, Seong-Soo;Kim, Choon Young;Kim, Gyo-Nam
    • Preventive Nutrition and Food Science
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    • v.21 no.3
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    • pp.227-235
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    • 2016
  • Obesity is a major risk factor for various metabolic diseases such as cardiovascular disease, hypertension, and type 2 diabetes mellitus. In this study, we prepared ethanol extracts from Agastache rugosa (ARE), Chrysanthemum zawadskii (CZE), Mentha arvensis (MAE), Perilla frutescens (PFE), Leonurus sibiricus (LSE), Gardenia jasminoides (GJE), and Lycopus coreanus (LCE). The anti-oxidant and anti-adipogenic effects were evaluated. The $IC_{50}$ values for ascorbic acid and LCE against 2,2-diphenyl-1-picrylhydrazyl radicals were $246.2{\mu}g/mL$ and $166.2{\mu}g/mL$, respectively, followed by ARE ($186.6{\mu}g/mL$), CZE ($198.6{\mu}g/mL$), MAE ($337.1{\mu}g/mL$), PFE ($415.3{\mu}g/mL$), LSE ($548.2{\mu}g/mL$), and GJE ($626.3{\mu}g/mL$). In non-toxic concentration ranges, CZE had a strong inhibitory effect against 3T3-L1 adipogenes (84.5%) than those of the other extracts. Furthermore, the anti-adipogenic effect of CZE is largely limited in the early stage of adipogenesis, and we revealed that the inhibitory role of CZE in adipogenesis is required for the activation of Wnt signaling. Our results provide scientific evidence that the anti-adipogenic effect of CZE can be applied as an ingredient for the development of functional foods and nutri-cosmetics for obesity prevention.