• Korean Journal of Plant Tissue Culture
• /
• v.28 no.6
• /
• pp.329-333
• /
• 2001

Callus and shoot formation from medicinal crop, Lycium chinense Mill. cv. 'Cheongyang', as influenced by various media, explant sources and plant growth regulators were investigated. The rate of shoots formation, number of shoots, and fresh weight of shoots were the best on MS medium followed by B$_{5}$, WPH, and SH. Callus induction was more effective in leaf than internode segments, and was the best on MS medium containing 0.5 mg/L NAA with 0.2 mg/L BA. Effects of plant growth regulators in shoot formation were more effective in BA than TDA combined with NAA. Shoot formation from callus induced in leaf and internode segments was the best on MS medium containing 0.01 mg/L NAA with 0.2 mg/L BA.

• Journal of Plant Biotechnology
• /
• v.6 no.3
• /
• pp.157-163
• /
• 2004

An efficient system for the regeneration of adventitious shoots from in vitro cultured leaf sections of Lycium chinense Miller was developed and silent somaclones from the regenerants detected with RAPD method. Among the eight media tested (B5, SH, N&N, 1/2MS, MS, 3/2MS, GD and WPM), and four cytokinins (BA, kinetin, 2ip and zeatin) with different concentrations (1, 5, 10, 20, 30 and 40 $\mu{M}$), 1/2 MS medium supplemented with 20 and 30 $\mu{M}$ zeatin showed the best regeneration frequency (100% and 93.7%) and higher average number of shoots (9.0 and 9.4). All regenerants easily elongated after subculturing on 1/4MS without growth stimulants and produced spontaneous adventitious roots from their basal parts. With phenotypically normal 40 regenerants, RAPD analysis with 15 different random primers was performed to examine the cryptic somaclonal variants. No substantial differences in banding patterns were found in the amplified polymorphic DNAs implying no DNA changes during dedifferentiation into adventitious shoots. However, one (OPF-4) of the 15 primers detected silent somaclonal variation in one regenerant in which two different polymorphic bands did not appear when compared with the rest regenerants. The results indicate that regenerantion via intervening callus phase can be used to establish true-to-type planting stocks for homogeneous population.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 1996.09a
• /
• pp.70-71
• /
• 1996

• Proceedings of the Zoological Society Korea Conference
• /
• 1997.10b
• /
• pp.200.2-200
• /
• 1997

No Abstract, See Full Text

• Proceedings of the PSK Conference
• /
• 2001.10a
• /
• pp.256.1-256.1
• /
• 2001

• Natural Product Sciences
• /
• v.15 no.2
• /
• pp.101-105
• /
• 2009

We previously reported that a novel cerebroside, LCC, isolated from the fruits of Lycium chinense (Solanaceae), significantly exerted hepatoprotective activity against both the carbon tetrachloride-induced and galactosamine-induced toxicities in primary cultures of rat hepatocytes. In the present study, we further attempted to determine the effect of LCC on hepatic fibrosis in animal model. Hepatic fibrosis was induced in rats by bile duct ligation/scission (BDL) for a period of 5 weeks. Treatment of BDL rats with LCC significantly reduced collagen deposition and the activities of serum alkaline phosphatase and ${\gamma}$-glutamyl transpeptidase. In addition, the LCC treatment of BDL rats significantly preserved the decreased hepatic glutathione as well as the activities of glutathione reductase and catalase in BDL rats. From the results, it can be speculated that LCC might exert antifibrotic activity in rats with BDL, in part, through the preservation of antioxidant enzymes and hepatic glutathione.

• Journal of Plant Biotechnology
• /
• v.29 no.4
• /
• pp.241-246
• /
• 2002

Somatic embryos or adventitious buds were formed from the segments of zygotic embryo of Lycium chinense Mill. on Murashige and Skoog (MS) medium supplemented with auxins (2,4-D, NAA, IAA) and / or cytokinins (zeatin, kinetin, BAP). Embryogenic callus formed on MS medium containing 0.5 mg/L 2,4-D and then differentiated into somatic embryos without transfer to hormone free medium. On the other hand, adventitious buds were formed on medium with 0.01 mg/L 2,4-D and 0.5 mg/L zeatin. Among various parts of zygotic embryos, the morphogenic potential was higher in the cotyledonary region, and the most organogenic potential was found in cotyledon followed by radicle, hypocotyl, and whole embryo. Histologically, bipolar structure of the heart-shaped embryos were confirmed and in adventitious buds only shoot apical meristems were found to exist.

• Weed & Turfgrass Science
• /
• v.5 no.2
• /
• pp.60-64
• /
• 2016

Investigation on weed flora in Lycium chinense upland fields was conducted to understand the current status of weed flora and establish the control measures. Investigation was conducted twice, May and June on 2015. From this investigation, 91 species of 30 families including 27 exotics were identified into 36 species of annuals, 28 species of biennials and 27 species of perennials. Dominance was the highest with Portulaca loeracea followed by Digitaria ciliaris, Stellaria media, Oxalis corniculata, Acalypha australis, Chenopodium album, Cyperus iria etc. in order. Chenopodium album was the highest in importance analysis and the followings were in order of Conyza canadensis, Conyza bonariensis, Senecio vulgaris, Amaranthus lividus, Sonchus asper etc.

• Korean Journal of Medicinal Crop Science
• /
• v.4 no.1
• /
• pp.58-63
• /
• 1996

This experiment was carried out to elucidate the effect of the rain shelter and insect net on the growth and yield in Lycium chinense 'Cheongyang Cultivar'. The yield cultivated at the condition of the rain shelter and insect net were increase $92{\sim}106$ percent as that of nature conditions resulted from better growth and decrease of fruit attacked by anthracnose and injured by Eriophyes kuko. It had not significant between the ventilation and non-ventilation.

• Korean Journal of Ecology and Environment
• /
• v.52 no.2
• /
• pp.171-177
• /
• 2019

Smart farm is a breakthrough technology that can maximize crop productivity and economy through efficient utilization of space regardless of external environmental factors. This study was conducted to investigate the optimal growth and physiological conditions of Chinese matrimony vine (Lycium chinense) with LED light sources in a smart farm. The light source was composed of red+blue and red+blue+white mixed light using a LED system. In the red+blue mixed light, red and blue colored LEDs were mixed at ratios of 1:1, 2:1, 5:1, and 10:1, with duty ratios varied to 100%, 99%, and 97%. The experimental results showed that the photosynthetic rate according to the types of light sources did not show statistically significant differences. Meanwhile, the photosynthetic rate according to the mixed ratio of the red and the blue light was highest with the red light and blue LED ratio of 1:1 while the water use efficiency was highest with the red and blue LED ratio of 2:1. The photosynthetic rate according to duty ratio was highest with the duty ratio of 99% under the mixed light condition of red+blue+white whereas the water use efficiency was highest with the duty ratio of 97% under the mixed light of red+blue LED. The results indicate that the light source and light quality for the optimal growth of Lycium chinense in the smart farm using the LED system are the mixed light of red+blue (1:1) and the duty ratio of 97%.