• 한국가축번식학회지
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• 제15권3호
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• pp.161-172
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• 1991

This study was conducted to research the endocrine mechanisms of postpartum anestrus and determine if the morphology of the CL could be related to function in Korean native goats. At parturition 48 goats were assigned to a nonsuckled group and a suckled group. Serum concentrations of luteinizing hormone(LH), follicle stimulating hormone(FSH), prolactin(PRL), estradiol-17$\beta$(E2) and cortisol were measured at various times after parturitionin the goats. The corpora lutea of pregnancy were examined by a light microscope on the 6th hour and the first, 3rd, 10th, 11th, and 21st days after parturition. The results were summarized as follows : Mean serum LH concentrations were lower after parturition in all treatments and increased gradually with the intervals after parturition(P<0.01). These values did not differ between groups. The levels of serum FSH were lower after parturition and tended to increase gradually between 2 and 21 days. The levels of serum FSH are not significantly different between the groups of goats. Two days after kidding mean levels of serum PRL began to fall in nonsuckling goats but increased in suckling goats. During 3 weeks serum PRL concentrations were different between nonsuckling and suckling goats(P<0.01). Three days after parturition the levels of serum E2 decreased in all treatments. From parturition to day 21 serum E2 concentrations were greater in nonsuckling than in suckling goats(P<0.01). At the sixth hour after parturition the structure of the CL was well preserved. At days 1 and 3 the blood vessels were sparcely distributed, whereas, at days 1 and 3 the blood vessels were sparcely distributed, whereas, at days 10, 11 and 21 tortuous larger vessels with thick walls were observed on the luteal tissue. At days 1, 3, 10, 11 and 21 after parturition the CL of pregnancy showed degeneration and the proportion of tissue occupied by intercelluar substances increased at days 21 postpartum. In conclusion, the present study has shown that regression of the CL of pregnancy is accelerated in the period after parturition and effectively completed within three weeks postpartum.

• Clinical and Experimental Reproductive Medicine
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• 제27권2호
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• pp.173-177
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• 2000

연구목적: 한국여성의 다낭성 난포증후군 환자에서 황체형성 호르몬 exon 2 유전자의 변이를 탐색하여 이들 변이와 질환과의 관련성 여부를 밝히고자 하였다. 연구재료 및 방법: 21명의 다낭성 난포증후군 환자를 대상으로 황체형성 호르몬 exon 2(Trp8Arg;TGG to CGG and Ile15Thr; ATC to ACC)의 변이를 탐색하였다. 혈액에서 Genomic DNA를 추출하여 PCR로 증폭한 후 RFLP 방법으로 변이형을 구분하였다. 결과: 황체형성 호르몬 exon 2의 변이형이 다낭성 난포증후군 환자에서 28.6%로 이미 조사된 바 있는 대조군의 16.7% 보다 약간 높게 나타났으나 통계적으로 유의한 차이는 없었다(p>0.05). 결론: 황체형성 호르몬 exon 2의 변이가 한국인의 다낭성 난포증후군 발병과 관련이 있는지를 밝히기 위해 더 많은 개체에 대한 연구가 요구된다.

• 한국가축번식학회지
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• 제25권2호
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• pp.155-162
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• 2001

본 실험은 한국재래산양에 있어서 분만 후 초기에 prostaglandin $F_{2{\alpha}}$(PG $F_{2{alpha}$)의 투여가 호르몬 농도 및 난포발육에 미치는 효과를 검토하기 위하여 수행되었던 바 그 결과는 다음과 같다. 1. 분만한 한국재래산양에 혈장중 PG $F_{2{\alpha}}$의 농도는 PG $F_{2{\alpha}}$처리 후 2일에 PG $F_{2{\alpha}}$처리군 (GR-1 및 GR-2)에서 각각 6.35$\pm$0.5 및 0.22$\pm$0.2pg/$m\ell$로서 저하한 후 점차 증가하여 4일 후 21.18$\pm$1.6 및 4.21$\pm$0.4 pg/$m\ell$로서 절정에 도달하였다. 2. 혈장중 PG $F_{2{\alpha}}$의 농도는 PG $F_{2{\alpha}}$ 처리 전 9.08$\pm$1.2 및 5.44$\pm$1.8 pg/$m\ell$였으며 처리후 농도는 다소 저하하였으나 처리 군간에 유의차는 없었다. 3. 혈장중 estradiol-l7$\beta$( $E_2$)농도는 PG $F_{2{\alpha}}$ 처리 후 2일까지 점차 감소한 후 3일에 증가하여 4일에 최대치에 도달하였다. 4. 혈장중 luteinizing hormone (나T)의 농도는 PG $F_{2{\alpha}}$ 처리 후 약간의 증가가 있었으나 유의한 차이는 없었다. 5. 혈장중 prolactin (PRL)의 농도는 PG $F_{2{\alpha}}$처리후 감소되었으나 저농도의 수준으로 남아 있었다. 6. PG $F_{2{\alpha}}$의 처리 후 소형 및 중형의 난포의 수는 거의 유사하였으나 4mm 이상 대형 난포의 수는 숫적로 증가하는 경향을 보였다. 이상의 결과로 보아 PG $F_{2{\alpha}}$ 및 estradiol-17$\beta$는 난포의 직경과 관계가 있다고 암시되며 PG $F_{2{\alpha}}$는 난포발육을 촉진하였다고 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권4호
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• pp.487-499
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• 2016

The aim of the present study was to examine the effects of testosterone (T) and estradiol-$17{\beta}$ ($E_2$) on the production of progesterone ($P_4$) by granulosa cells, and of the $E_2$ on the production of $P_4$ and T by theca internal cells. In the first experiment, granulosa cells isolated from the largest ($F_1$) and third largest ($F_3$) preovulatory follicle were incubated for 4 h in short-term culture system, $P_4$ production by granulosa cells of both $F_1$ and $F_3$ was increased in a dose-dependent manner by ovine luteinizing hormone (oLH), but not T or $E_2$. In the second experiment, $F_1$ and $F_3$ granulosa cells cultured for 48 h in the developed monolayer culture system were recultured for an additional 48 h with increasing doses of various physiological active substances existing in the ovary, including T and $E_2$. Basal $P_4$ production for 48 h during 48 to 96 h of the cultured was about nine fold greater by $F_1$ granulosa cells than by $F_3$ granulosa cells. In substances examined oLH, chicken vasoactive intestinal polypeptide (cVIP) and T, but not $E_2$, stimulated in a dose-dependent manner $P_4$ production in both $F_1$ and $F_3$ granulosa cells. In addition, when the time course of $P_4$ production by $F_1$ granulosa cells in response to oLH, cVIP, T and $E_2$ was examined for 48 h during 48 to 96 h of culture, although $E_2$ had no effect on $P_4$ production by granulosa cells of $F_1$ during the period from 48 to 96 h of culture, $P_4$ production with oLH was found to be increased at 4 h of the culture, with a maximal 9.14 fold level at 6 h. By contrast, $P_4$ production with cVIP and T increased significantly (p<0.05) from 8 and 12 h of the culture, respectively, with maximal 6.50 fold response at 12 h and 6, 48 fold responses at 36 h. Furthermore, when $F_1$ granulosa cells were precultured with $E_2$ for various times before 4 h culture with oLH at 96 h of culture, the increase in $P_4$ production in response to oLH with a dose-related manner was only found at a pretreatment time of more than 12 h. In the third experiment, theca internal cells of $F_1$, $F_2$ and the largest third to fifth preovulatory follicles ($F_{3-5}$) were incubated for 4 h in short-term culture system with increasing doses of $E_2$. The production of $P_4$ and T by theca internal cells were increased with the addition of $E_2$ of $10^{-6}M$. These increases were greater in smaller follicles. These results indicate that, in granulosa cells of the hen, T may have a direct stimulatory action in the long term on $P_4$ production, and on $E_2$ in long-term action which may enhance the sensitivity to LH for $P_4$ production, and thus, in theca internal cells, $E_2$ in short term action may stimulate the production of $P_4$ and T.