• Asian Journal of Atmospheric Environment
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• v.7 no.2
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• pp.105-113
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• 2013

We constructed and tested an airborne peroxyacetyl nitrate (PAN) monitoring system based on luminol chemiluminescence detection with fast gas chromatography. This system allowed for simultaneous measurement of PAN and nitrogen dioxide ($NO_2$) with a time resolution of <2 min. Actual sample masses within the fixed volume sample loop at various altitudes and temperatures were adjusted to standard atmosphere, using measured pressures and temperatures. The airborne PAN measurement system was evaluated during two field studies above the southern Korean Peninsula in August and October 2009. The detection limit based on the ISO approach was 0.035 ppbv PAN, well below the observed concentrations of 0.185-1.49 ppbv during these studies. Under these conditions, the PAN mixing ratios were positively correlated with $O_x$ ($O_x=O_3+NO_2$), with slopes varying between 0.014 and 0.033 and intercepts between 22.6 and 55.1 ppbv $O_x$. The intercepts corresponded roughly to background $O_x$ mixing ratios in central Europe; however, the slopes were above the range of slopes reported in other studies. We also enhanced the durability, safety, and ease of maintenance of the PAN monitoring system by redesigning the structure of the conventional luminol cell.

• Proceedings of the KIEE Conference
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• 2002.07c
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• pp.1552-1554
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• 2002

본 논문에서는 실리콘을 기초로 한 포토다이오드를 제작하여 현재 바이오센서에서 이용되고 있는 luminol 화학 발광을 전기적으로 검출하였다. 우선, 실리콘 웨이퍼에 이온주입을 통해 p-n 접합을 형성하고 Al 전극을 형성시켜 포토다이오드를 제작하였다. PDMS을 passivation 막으로 사용하여 horseradish peroxidase(HRP) 농도 변화에 따른 발광의 최적조건과 luminol, HRP가 섞인 용액에 서로 다른 양의 과산화수소를 넣어 발광이 최대로 발생하는 혼합비를 측정하였다. 최종적으로 2mM luminol, 25U HRP 조건 하에서 과산화수소 농도에 따른 화학 발광을 측정하였으며, $10{\mu}M$부터 $500{\mu}M$ 범위의 과산화수소 농도 변화에 따른 포토다이오드의 감도는 단위 면적당 23.429pA/ ${\mu}M$이다.

• Analytical Science and Technology
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• v.31 no.2
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• pp.71-77
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• 2018

Detection of bloodstains is a very important process in scientific investigations, and luminol is often used for the detection of bloodstains that are not visible. Recently, new preparation methods of blood detection reagents based on luminol (BloodFlareA, B) were developed and reported to have higher active persistence and to be more economical than conventional blood detection reagent, BlueStar forensic. In this paper, we tested the specificity and effect of the BloodFlares (A and B) on DNA and compared them with those of BlueStar forensic. False positive results for the BloodFlares were not observed in semen, saliva, vaginal fluids, urine, sweat, and nasal discharge, but were observed in $CuSO_4$, $FeSO_4$, and bleach solutions, and the observed patterns were similar to those of BlueStar forensic. The effect on DNA was determined by analyzing the DNA yield, degradation index, and DNA profiling. Based on these results, we concluded that the BloodFlares based on luminol do not affect DNA stability and are applicable in forensics.

• ALGAE
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• v.28 no.3
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• pp.289-296
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• 2013

For the quantitative detection of algal toxin, microcystin, a chemiluminescence immunochromatographic assay method was developed. The developed system consists of four parts, chemiluminescence assay strip (nitrocellulose membrane), horse radish peroxidase labeled microcystin monoclonal antibodies, chemiluminescence substrate (luminol and hydrogen peroxide), and luminometer. The performance of the chemiluminescence immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. The detection limit of chemiluminescence immunochromatographic assay system is several orders of magnitude lower than with HPLC. The chemiluminescence immunochromatography and HPLC results correlated very well with the correlation coefficient ($r^2$) of 0.979.

• Applied Chemistry
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• v.15 no.1
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• pp.21-24
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• 2011

A sensitive silver nanoparticles (Ag NPs) enhanced chemiluminescence (CL) method is reported for the determination of mandelic acid (MA). This method is based on the luminol-KIO₄ system catalyzed by Ag NPs to produce CL spectra. Prepared Ag NPs were characterized by UV-visible spectra and TEM image. Under optimal condition, CL spectra of the system were responded linearly with the concentration of MA in the range of 2.5×10^-9 to 2.0×10^-8 mol L^-1 (r=0.9989) with a detection limit of 1.2×10^-10 mol L^-1. The relative standard deviation of 1.0×10^-7 mol L^-1 MA was found 1.45 (n=9).

• Applied Chemistry
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• v.15 no.1
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• pp.37-40
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• 2011

A chemiluminescent method with silver nanoparticles for determination of L-alanine has been presented. The chemilumiscence intensity was further enhanced by silver nanoparticles in the luminol system by its catalytic role. The silver nanoparticles enhanced chemiluminescent method is applicable for the determination of an amino acid such as alanine. When alanine was introduced to the luminol system with silver nanoparticles, chemiluminescence intensity was reduced with the concentration of the added alanine. The effects of pH, concentrations of luminol, hydrogen peroxide and silver nanoparticles on the chemiluminescence intensity were investigated. The calibration curve for L-alanine was linear over the range from 6.60×10^-8 M to 4.00×10^-7 M, coefficient of correlation was 0.996 and detection limit was 3.5×10^-9 M under the optimal conditions of 4.0×10^-3 M, 4.0×10^-2 M, 4.0×10^-4 M, 12.8 for the concentration of luminol, H₂O₂, silver nanoparticles and pH, respectively.

• Applied Chemistry
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• v.15 no.2
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• pp.113-116
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• 2011

A sensitive and selective determination method of Fe(II) ion by luminol-H₂O₂ system using a chelating reagent has been presented. A metal ion-chelating ligand complex such as Fe(II)-diethylenetriamine pentaacetic acid (DTPA) produced higher chemiluminescence (CL) intensity as well as longer lifetime in luminol-H₂O₂ system than metal exist as free ions. Furthermore, the catalytic activity of Cu(II) and Pb (II) complexes with chelating reagents in luminol-H₂O₂ system was lost since chelating reagents act as a masking agent although free Cu(II) and Pb(II) ions have high catalytic activity. On the optimized conditions, the calibration curve of Fe(II) ion was linear over the range from 1.0×10^-7 to 2.0×10^-5 M with correlation coefficient of 0.996. The detection limit was calculated to be 4.0×10^-8 M.

• Analytical Science and Technology
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• v.25 no.3
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• pp.171-177
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• 2012

A determination method of aromatic amino acids such as trytophan (Trp), tyrosine (Tyr), and phenylalanine (Phe) using luminol-$H_2O_2$-Cu(II) system has been presented. In the presence of an aromatic amino acid, the enhanced chemiluminescence (CL) intensity of luminol-$H_2O_2$-Cu(II) system was obtained by forming a complex between Cu(II) and the amino acid. Based on the above phenomenon, a sensitive and fast determination of three aromatic amino acids was performed using the CL method in batch-type detection system. To optimize determination conditions, the kinetic influence of an aromatic amino acid on the luminol-$H_2O_2$-Cu(II) system and the effects of $H_2O_2$ and Cu(II) concentration, pH, and buffers were investigated. Under the optimized conditions, the calibration curve was linear over the range from $1.0{\times}10^{-6}$ to $2.0{\times}10^{-5}\;M$ for Trp, $1.0{\times}10^{-6}$ to $2.0{\times}10^{-5}\;M$ for Try, and $2.0{\times}10^{-6}$ to $2.0{\times}10^{-5}\;M$ for Phe, respectively. In this range, reproducibility (RSD, n = 4) of Trp, Try, and Phe were 3.21%, 2.64%, and 2.48%, respectively. The limit of detection ($3{\sigma}/s$) was calculated to be $6.8{\times}10^{-7}\;M$ for Trp, $5.7{\times}10^{-7}\;M$ for Try, and $9.6{\times}10^{-7}\;M$ for Phe.

• Analytical Science and Technology
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• v.24 no.4
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• pp.243-248
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• 2011

A selective determination method of mercury (II) ion in aqueous solution by luminol-based chemiluminescence system (luminol CL system) has been developed. Determination of metal ions such as copper (II), iron (III), chromium (III) ion in solution by the luminol CL system using its catalytic role in the reaction of luminol and hydrogen peroxide has been reported by several groups. In this study, the catalytic activity of mercury (II) ion in the reaction of luminol and hydrogen peroxide was observed by the enhanced CL intensity of the luminol CL system. Based on this phenomenon, experimental conditions of the luminol CL system were investigated and optimized to determine mercury (II) ion in aqueous solution. While mercury (II) ion in mixed sample solution containing mercury (I) and (II) ions highly enhanced the CL intensity of the luminol CL system, the mercury (I) ion could not enhanced the CL intensity. Thus selective determination of the mercury (II) ions in a mixture containing mercury (I) and (II) ions could be achieved. Each concentration of mercury (I) and (II) ions in aqueous solution can be obtained from the results of the CL method that give the concentration of only mercury (II) ion and the inductively coupled plasma (ICP) method that give the total concentration of mercury ions. On the optimized conditions, the calibration curve of mercury (II) ion was linear over the range from $1.25{\times}10^{-5}$ to $2.50{\times}10^{-3}M$ with correlation coefficient of 0.991. The detection limit of mercury (II) ion in aqueous solution was calculated to be $1.25{\times}10^{-7}M$.

• Bulletin of the Korean Chemical Society
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• v.32 no.2
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• pp.639-644
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• 2011

A simple and highly sensitive chemiluminescence method to determine norfloxacin (NFLX) has been proposed by measuring the chemiluminescence (CL) intensities using a flow injection (FI) system. The CL intensity of the luminol-$H_2O_2$ system is strongly enhanced by the addition of Cu (II) in alkaline condition. The CL intensity is substantially increased after the injection of NFLX into the luminol-$H_2O_2$-Cu (II) system. The enhancement effect is attributed to a catalytic effect of Cu (II) due to the interaction with NFLX which forms a complex with the catalyst. Under the optimal conditions, the sensitizing effect of the CL intensity is proportional to the concentration of NFLX in the range of $1.5{\times}10^{-9}-5.9{\times}10^{-7}molL^{-1}$ (r = 0.9994) with a detection limit ($3{\sigma}$) of $2.98{\times}10^{-10}molL^{-1}$. The proposed method had good reproducibility with the relative standard deviation (RSD, n = 5) of 1.6% for $1{\times}10^{-7}molL^{-1}$ of NFLX. The possible reaction mechanism of the CL reaction is also discussed. This method has been successfully applied for the determination of trace amount of NFLX in pharmaceutical preparations and serum samples.