• Title/Summary/Keyword: Low temperature transfer method

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Ultrarapid Freezing of Mouse Ova (생쥐난자의 초급속동결)

  • 박영식;서태광;이택후;전상식
    • Journal of Embryo Transfer
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    • v.10 no.3
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    • pp.203-208
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    • 1995
  • This study was carried out to efficiently use the ultrarapid freezing method in the cryopreservation of mouse ova. For this, the effects of dehydration method, oval vigour and $0^{\circ}C$ controlling method on post-thawing viability were investigated. Fresh mouse ova were dehydrated in mPBS with 3.5M DMSO and /or 0.25M sucrose, and directly immersed in L$N_2$ for ultrarapidly freezing. The frozen ova were thawed at 37$^{\circ}C$, rehydrated in mPBS with 0.25M sucrose, and then repeatedly washed in HAM's Fl0 before evaluating the morphological normality of frozen-thawed ova. The results obtained showed that there was difference between treatments in a experiment. 1) The post-thawing viability of ova dehydrated in multi-step (48.4$\pm$13.8%) was higher than that of ova in two-step (40.9$\pm$14.0%). 2) The post-thawing viability of fertilized ova (87$\pm$14.0%) was significantly(p<0.0l) higher than that of unfertilized ova (5.4$\pm$5.4%). 3) The post-thawing viability of ova dehydrated and rehydrated using a cooling machine (95.8$\pm$4.2%) was significantly(p<0.05) higher than that on ice(84.1$\pm$9.9). In conclusion, in order to efficiently cryopreserve ova in vitro with ultrarapidly freezing method, highly viable embryos should be selected, heavy osmotic shock to the dehydrating ova should be avoided, and embryos in high osmotic condition were dehydrated and rehydrated in a constantly low temperature.

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Natural Convection in a Rectangular Enclosure with Localized Heating from Below (사각공간내에서의 부분바닥가열에 의한 자연대류에 관한 연구)

  • Han, H.T.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.7 no.2
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    • pp.287-297
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    • 1995
  • In this study, the geometry consists of a two-dimensional rectangular enclosure with localized heating from below. The size and the location of the heater on the floor has been varied, and one of the vertical walls remains at a low temperature simulating a cold window. The governing equations for momentum, energy and continuity, which are coupled with turbulent equations have been solved using a finite volume method. A low Reynolds number $k-{\varepsilon}$ model has been incorporated to solve the turbulent kinetic energy and the dissipation rate. The heat transfer characteristics and the thermal environmental characteristics of the room have been obtained for various system parameters in a room with a partially heated floor.

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A Study on the Prediction of Combustion Gas Behavior Induced by Fire in a Building (건물내 화재에 의한 연소가스 거동 예측에 관한 연구)

  • Pak, H.Y.;Park, K.W.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.6 no.3
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    • pp.267-281
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    • 1994
  • The Combustion gas behavior induced by fire in a building is numerically investigated. The typical building for this analysis is partially divided by a vertical baffle projecting from the ceiling. The solution procedure includes the low Reynolds number ${\kappa}-{\varepsilon}$ model for the turbulent flow and the discrete ordinates method is used for the calculation of radiative heat transfer equation. The effects of the location and size of fire source and baffle length on velocity and temperature distributions, species mass fraction and flame location are analyzed. As the results of this study, it is found that the case when the fire source is located at the vertical wall is more dangerous than at the bottom wall in view of the combustion products and flame location. It is also found that the radiation effect cannot be neglected in analyzing the building in fire.

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Development of a Heat Regenerator Using High Temperature Phase Change Material : Part I Prediction of Heat Transfer Phenomena in a Single Module of Phase Change Material (초고온 상변화 물질을 이용한 열회수장치 개발:Part I 축열재 모듈의 열전달 현상 해석)

  • 박준규;서경원;김상진
    • Journal of Energy Engineering
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    • v.2 no.3
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    • pp.258-267
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    • 1993
  • A mathematical model has been developed to describe heat transfer phenomena in a PCM (phase change material) module for development of an energy recovery system. The PCM module, melting point of which is around 1673 K, consists of silicon(96.8%), aluminium(2.7%) and marginal amounts of impurities such as Ca, Fe and Ti. The module is covered by a capsule that consists of SiC(58%) and graphite(42%). Physical properties that are required for model predictions were cited from the references. The apparent capacity method and the postiterative method wert used in the mathematical model to describe the phase changing mechanism. Temperature and velocity of fluid are the major variables in the model calculation. For the gas temperature of 1773 K that simulates real operating conditions, the prediction shows that PCM is rapidly melted to axial direction. However, for the gas temperature of 3000 K that is higher than the real conditions, PCM is melted rapidly to the radial direction. The gas velocity has no influence on the melting phenomena of the PCM except when the gas velocity is relatively low. At the low gas velocity asymmetry of the temperature profiles in PCM is obtained.

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Analysis of a Cryogenic Nitrogen-Ambient Air Heat Exchanger Including Frost Formation (착상을 고려한 극저온 질소-대기 열교환기의 해석)

  • 최권일;장호명
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.12 no.9
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    • pp.825-834
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    • 2000
  • A heat exchanger analysis is performed to investigate the heating characteristics of cryogenic nitrogen by ambient air for the purpose of cryogenic automotive propulsion. The heat exchanger is a concentric triple-passage for supercritical nitrogen, and the radial fins are attached on the outermost tube for the crossflow of ambient air. The temperature distribution is calculated for the nitrogen along the passage, including the real gas properties of nitrogen, the fluid convections and the conductions through the tube walls and the fins. Since the wall temperature of the outer (ambient side) tube is very low in most cases, a heavy frost can be formed on the surface, affecting the heat exchange performance. By the method of the similarity between the heat and the mass transfer of moist air, the frost growth and the time-dependent effectiveness of the heat exchanger are calculated for various operating conditions. It is concluded that the frost formation can augment the heating of nitrogen during the initial period because of the latent heat, then gradually degrades the heat exchange because of the increased thermal resistance. Practical design issues are discussed for the flow rate of nitrogen, the velocity and humidity of ambient air, and the sizes of the fin.

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Computational Thermal Flow Analysis of a Cabin Cooler for a Commercial Vehicle (상용차용 캐빈냉방기의 전산 열유동 해석)

  • Kim, J.K.;Oh, S.H.
    • Journal of Power System Engineering
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    • v.16 no.2
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    • pp.17-23
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    • 2012
  • The steady three-dimensional computational thermal flow analysis using standard k-${\varepsilon}$ turbulence model was carried out to investigate the heat transfer characteristics of a cabin cooler for a commercial vehicle. The heat exchanging method of this cabin cooler is to use the cooling effect of a thermoelectric module. In view of the results so far achieved, the air system resistance of a cabin cooler is about 12.4 Pa as a static pressure, and then the operating point of a cross-flow fan considering in this study is formed in the comparatively low flowrate region. The air temperature difference obtained from the cold part of an thermoelectric module is about $26^{\circ}C$, and the cooling water temperature difference obtained from the hot part of an thermoelectric module is about $3.5^{\circ}C$.

A Thermoelectric Energy Harvesting Circuit For a Wearable Application

  • Pham, Khoa Van;Truong, Son Ngoc;Yang, Wonsun;Min, Kyeong-Sik
    • Journal of IKEEE
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    • v.21 no.1
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    • pp.66-69
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    • 2017
  • In recent year, energy harvesting technologies from the ambient environments such as light, motion, wireless waves, and temperature again a lot of attraction form research community [1-5] due to its efficient solution in order to substitute for conventional power delivery methods, especially in wearable together with on-body applications. The drawbacks of battery-powered characteristic used in commodity applications lead to self-powered, long-lifetime circuit design. Thermoelectric generator, a solid-state sensor, is useful compared to the harvesting devices in order to enable self-sustained low-power applications. TEG based on the Seebeck effect is utilized to transfer thermal energy which is available with a temperature gradient into useful electrical energy. Depending on the temperature difference between two sides, amount of output power will be proportionally delivered. In this work, we illustrated a low-input voltage energy harvesting circuit applied discontinuous conduction mode (DCM) method for getting an adequate amount of energy from thermoelectric generator (TEG) for a specific wearable application. With a small temperature gradient harvested from human skin, the input voltage from the transducer is as low as 60mV, the proposed circuit, fabricated in a $0.6{\mu}m$ CMOS process, is capable of generating a regulated output voltage of 4.2V with an output power reaching to $40{\mu}W$. The proposed circuit is useful for powering energy to battery-less systems, such as wearable application devices.

Numerical Study of Characteristic of Heat and Mass Transfer in Planar Membrane Humidifier According to Flow Direction (연료전지용 판형 막 가습기의 유동방향에 따른 열 및 물질전달 특성에 관한 해석적 연구)

  • Yun, Sungho;Byun, Jae Ki;Choi, Young Don
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.5
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    • pp.503-511
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    • 2013
  • The humidifying supply gas is important in terms of the performance efficiency and membrane life improvement of a PEM fuel cell. A planar membrane humidifier is classified as a cross-flow and counter-flow type depending on the flow direction, and heat and mass transfer occur between the plate and the membrane. In this study, the changes in heat and mass transfer for various inlet temperatures and flow rates are compared according to the flow direction by using the sensible and latent ${\varepsilon}$-NTU method. The obtained results indicate that the counter flow shows higher heat and mass transfer performance than the cross flow at a low flow rate, and the difference in performance decreases as the flow rate increases. Furthermore, changes in the mass transfer performance decrease considerably with a nonlinear increase in the inlet temperature, and variations of the heat transfer performance are small.

Analysis of the Evaporation Behavior of Resin Droplets in UV-Nanoimprint Process (UV 나노임프린트 공정에서의 수지 액적 증발 거동 분석)

  • Choi, D.S.;Kim, K.D.
    • Transactions of Materials Processing
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    • v.18 no.3
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    • pp.268-273
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    • 2009
  • Ultraviolet nanoimprint lithography (UV-NIL), which is performed at a low pressure and at room temperature, is known as a low cost method for the fabrication of nano-scale patterns. In the patterning process, maintaining the uniformity of the residual layer is critical as the pattern transfer of features to the substrate must include the timed etch of the residual layer prior to the etching of the transfer layer. In pursuit of a thin and uniform residual layer thickness, the initial volume and the position of each droplet both need to be optimized. However, the monomer mixtures of resin had a tendency to evaporate. The evaporation rate depends on not only time, but also the initial volume of the monomer droplet. In order to decide the initial volume of each droplet, the accurate prediction of evaporation behavior is required. In this study, the theoretical model of the evaporation behavior of resin droplets was developed and compared with the available experimental data in the literature. It is confirmed that the evaporation rate of a droplet is not proportional to the area of its free surface, but to the length of its contact line. Finally, the parameter of the developed theoretical model was calculated by curve fitting to decide the initial volume of resin droplets.

Parametric Study on the $LiBr-H_2O$ Absorption Process on Horizontal Tubes (수평원관상의 $LiBr-H_2O$ 흡수특성에 대한 연구)

  • Min J. K.;Choi D. H.
    • Journal of computational fluids engineering
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    • v.5 no.1
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    • pp.33-42
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    • 2000
  • The LiBr-H₂O absorption process on a horizontal tube has been analyzed numerically. The flow field, which was calculated in the authors' previous study by solving the fully elliptic Navier-Stokes equations with accurate free-surface-tracking method, is used to solve the temperature and concentration distributions in the absorption film. With the assumption that the absorbent is linear, calculations have been made for various inlet temperature and flow-rate conditions. For low inlet temperature, the absorption rate is large in the upstream region but the mean temperature also increases and as a result the absorption decreases as the film flows to downstream while high-inlet-temperature case does the opposite. The difference in the absorption rate due to the inlet temperature change becomes smaller in the downstream than that in the upstream. For large flow rate, the heat transfer to the wall becomes poor due to the thick film and so does the absorption rate. The analyses have also been carried out for multiple tube arrangement and the results show that the absorption rate converges after a few tube rows.

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