• Journal of Nutrition and Health
• /
• 제10권1호
• /
• pp.10-17
• /
• 1977

This study was designed to compare the Biological effects of the cereals and legumes Proteins on albino rats. Fifty weaning albino rats weighing $49\;gr{\pm}2$ in both sexes were divided into 5 groups and fed corn crude protein diet, wheat crude protein diet, soybean protein diet, yeast diet and 30% sugar-casein diet for the control group. The protein contains same levels with isocaloric values each diet. After 12 weeks the rats were sacrificed for chemical analysis and the results are as follow ; 1. The highest food consumption was found in the groups of yeast and soybean, and the lowest was found in the groups of wheat diet and corn diet. 2. The groups of yeast & soybean protein showed the highest body weight increase, while the groups of wheat diet and corn diet showed the lowest. 3. Highly significant difference was found between the standard group and the groups of corn protein and soybean protein in Food Efficiency Ratio. (F. E. R.) (p<0.01). 4. Protein Efficiency Ratio showed a similar pattern as the F.E.R., however, there was no significant differences among the groups. 5. The kind of diets did not influence the hematology of the subjects. 6. The rate of nitrogen retention of male standard group was lower compared with all the experimental groups (p<0.01), and that of female soybean group was higher than any other groups (p<0.01). 7. Female corn diet group had the lowest organ weights, as found in the growth rate, which was significant (p<0.01). In the male corn diet group only the kidney showed significantly low in weight (p<0.01), and the spleen of male yeast group was also shown low with significance (p<0.01). 8. Nitrogen retentions of the liver and muscle in male corn diet group were lower than any other groups (p<0.05), and the brain nitrogen content of female standard group was high with significance (p<0.01). According to the results above, yeast and soybean protein can be regarded as a nutritious and also inexpensive protein sources.

• 한국수산과학회지
• /
• 제9권4호
• /
• pp.257-259
• /
• 1976

잉어를 저밀도로 방양하고 동시에 사료중의 동물성단백질을 줄이는 실험을 하였다. 수면적 $454m^2$의 못 3개에 각 못에 평균 81.2g되는 보통잉어 72미와 평균 37.89g 되는 비단잉어 28미를 방양 189일간 사육한 결과, 동물질 $20\%$ 사료의 못에서는 보통잉어가 평균 776.2g, 비단잉어 평균 $504.2g$, 총평균, 701.1g으로 자라고, $35\%$구에서는 각각 792.9g, 539.1g, 730.1g, $50\%$에서는 각각 983.7g, 630.4g, 887.0g으로 자랐다. 이 실험에서 1ha당 생산고가 $20\%$구에서 1,352kg, $35\%$구에서, 1,495kg, $50\%$구에서 1,447kg였으므로 양어지에서의 보통생산고의 1/2에 미달되었으며 이 정도의 저밀도양어에서는 사육단백질의 량을 감소시켜서는 안된다는 결론이 나온다.

• Animal Bioscience
• /
• 제36권11호
• /
• pp.1718-1726
• /
• 2023

Objective: The objective of this study was to investigate the effect of supplementation with enzymolytic soybean meal (ESBM) on broilers fed low crude protein (CP) diets. Methods: A total of 360 one-day-old broilers were randomly assigned to six treatments with 6 replicates per treatment and 10 chicks per replicate for a period of 42 days. Chicks were fed a basal standard high-CP diet as a positive control (PC), a low-CP diet (reducing 10 g/kg CP from the PC) as a negative control (NC), or an NC + 0.5%, 1.0%, 1.5%, or 2.0% ESBM diet. Results: Compared to chicks fed the PC, chicks fed the NC had a decreased body weight gain (BWG, p<0.05) from 1 to 42 days, but supplementation with 2.0% ESBM restored BWG (p<0.05) and even linearly improved the feed conversion rate (FCR, p<0.05). Digestibility of CP and ether extract was increased (p<0.05) in chicks fed a 1.0% ESBM diet compared to the PC. With increasing levels of ESBM, nitrogen (N) excretion decreased (p<0.05). The addition of ESBM to the diet did not affect (p>0.05) serum concentrations of total protein, albumin and total cholesterol but led to a descending trend in triglycerides and an ascending trend in calcium and urea N at 42 days (p<0.10). There were no differences (p>0.05) in villus height (VH), crypt depth (CD), and VH/CD (V/C) of the duodenum and jejunum between the PC and NC at both 21 days and 42 days, while increasing dietary ESBM levels linearly (p<0.05) decreased CD and increased V/C of the duodenum and jejunum at both 21 days and 42 days. Conclusion: The findings indicated that ESBM could be used in broiler low-CP diets to improve production performance, decrease N excretion, and enhance intestinal health.

• Asian-Australasian Journal of Animal Sciences
• /
• 제10권1호
• /
• pp.1-7
• /
• 1997

This experiment was conducted with total 960 brown layers, consisted of 8 treatment to investigate the effects of dietary chromium as chromium picolinate on egg production, egg quality, nutrient utilizability, serum traits and mortality in brown layers. Layers were fed diets with two levels of dietary protein (14% and 16%) and supplemented with 0, 200, 400, 800 ppb/kg of chromium as chromium picolinate, respectively. The highest egg production, egg weight and egg mass were found in 800 ppb chromium picolinate supplementation group with high protein level (16%) (p < 0.05). Although there was no significant difference, layers receiving 400 ppb of chromium picolinate with high protein (16%) represented the lowest broken eggs. The utilization of energy, dry matter and crude protein of 400 ppb chromium picolinate group with low protein level (14%) were significantly higher than those of control or other chromium picolinate group (p < 0.05). 400 ppb chromium picolinate with low protein level (14%) showed the lowest serum glucose concentration. But serum glucose concentrations in all treatments showed no significant differences. Present date revealed that the lowest serum cholesterol concentration of layers was found at 400 ppb chromium picolinate group with high protein level (16%) (p < 0.05). Crude protein content in yolk was significantly higher in eggs of layers received 800 ppb chromium picolinate and the lowest in eggs from layers received 400 ppb chromium picolinate among chromium picolinate levels (p < 0.05). Mortality was remarkably decreased by chromium picolinate supplementation and the lowest mortality value was found in layres receiving 800 ppb chromium picolinate with high protein level.

• BMB Reports
• /
• 제28권5호
• /
• pp.443-450
• /
• 1995

Cloned staphylococcal peptidoglycan hydrolase was used in determining the physiological characteristics of peptidoglycan hydrolase. This enzyme hydrolyzed the bacterial cell walls and released the N-terminal alanine, but not the reducing groups. This cloned gene product was localized in the cytoplasm of transformed Escherichia coli. Activity gels indicated the enzyme had an Mr of about 54,000, which was consistent with the deduced Mr from sequencing of the cloned gene. The activity bound to CM-cellulose but not DEAE-cellulose resin, indicating it as a basic protein. Enhanced enzyme activity in a low concentration of cations, and inhibited enzyme activity in a solution with dissolved phospholipids, suggested that the activity and the availability of this basic protein may be regulated between negatively charged and positively charged cellular molecules. The activity against boiled crude cell wall was much greater than against purifed cell wall, suggesting protein associated with crude cell wall may aid in the binding of the peptidoglycan hydrolase The cloned peptidoglycan hydrolase showed positive activity on whole cells of some lysostaphin-resistant coagulase-negative staphylococci. The cloned enzyme may be an alternative for lysostaphin for lysis of staphylococci.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권3호
• /
• pp.421-428
• /
• 2011

Low-fat sausages with or without 10% hydrated rice flour were made from duck, chicken and pork and their physical and sensory properties were compared. Results showed that moisture content did not differ significantly among the sausage batters. However, crude protein, crude fat and total ash content were significantly lower in the group with added rice flour compared with the no flour group. Crude protein and crude fat were the highest in pork sausages without rice flour (p<0.05). Adding 10% rice flour reduced total expressible fluid in all meat type sausages. Cooking loss was also decreased when 10% rice flour was used in making sausages from chicken and pork. However, no changes in cooking loss were found in duck meat by adding rice flour. Again, the highest cooking loss was in pork sausages without rice flour and lowest in chicken sausages with 10% rice flour. The pH of the meat from different animal species differs significantly, although no significant difference was found within meat types with or without rice flour. Lightness ($L^*$) increased, while redness ($a^*$) decreased with adding rice flour in all meat type sausages. Results showed that hardness was significantly reduced when 10% rice flour was added to pork, chicken and duck meat (p<0.05). This may be due to increased water retention of rice flour after cooking. Sensory evaluation indicated that the overall acceptability of pork and chicken sausages with or without rice flour was the same, but duck sausages without rice flour had the highest off-flavor score among the sausages. Addition of rice flour increased the overall acceptability of duck sausage to that of pork and chicken sausages.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권12호
• /
• pp.1715-1720
• /
• 2005

Two promising varieties of forage sorghum viz. HD-15 and J. Sel-10 were identified for their higher forage yield (350-400 quintals per hectare) compared to traditional/existing variety, HC-136 at Indian Grassland and Fodder Research Institute, Jhansi. Silage of these three forage sorghum varieties viz., HD-15, J.Sel-10 and HC-136 were prepared in three concrete silos for their nutritional evaluation in sheep. Twelve adult Muzaffarnagari Ewes, divided into three equal groups of 4 each, were offered a particular variety of sorghum silage ad libitum over a period of 30 days followed by a 6 day metabolism trial and a collection of rumen liquor and blood samples. The HD-15 variety of forage sorghum silage contained higher crude protein (CP) than both the J.Sel-10 and HC-136. Average daily dry matter (DM) intake (% of live weight) differed significantly (p<0.05) in sheep that were fed the different varieties of silage, with the maximum amount in HD-15 (2.55) followed by J.Sel-10 (2.49) and HC-136 (1.84). The average apparent digestibility of all the nutrients was low in the sheep that were fed the HC-136 variety of sorghum silage when compared to both HD-15 and J.Sel-10. However, digestibility of organic matter (p<0.05) and crude protein (p<0.01) was significantly low in the HC-136 variety. The average daily nitrogen retention was-0.19, 2.15 and 0.42 g in HC-136, HD-15 and J.Sel-10, respectively which differed significantly (p<0.01). The average digestible crude protein and total digestible nutrient (TDN) contents (%) of the silage varieties were higher in HD-15 (3.14 and 55.3) than HC-136 (0.25 and 58.6) and J.Sel-10 (1.58 and 55.3) varieties. On comparing to the maintenance requirements (ICAR, 1985) for dry matter, crude protein and total digestible nutrients, both the improved varieties (HD-15 and J.Sel-10) almost met the requirements except crude protein, which met only 73.3% of the requirement by J.Sel-10, while the HC-136 variety was unable to meet the requirements. The average rumen pH and total volatile fatty acids concentrations were comparable among the groups. However, concentrations of nitrogen metabolites were higher in the animals fed the HD-15 variety of silage. Varieties of sorghum silage also did not have any influence on the concentration of the blood metabolites. It was concluded that the HD-15 variety of sorghum silage was nutritionally superior to both J.Sel-10 and HC-136 silage.

• Fisheries and Aquatic Sciences
• /
• 제5권3호
• /
• pp.172-177
• /
• 2002

This study was conducted to investigate the utilization of Sargassum meal in the diet on juvenile ayu (Plecoglossus altivelis) reared in seawater. White fish meal and wheat flour were used as the dietary protein and carbohydrate sources in the control diet. Wheat flour in the control diet was replaced with $5\%$ and $10\%$ Sargassum meal. Three replicate groups of fish average weighing 4.0 g were fed one of three isonitrogenous$(45\%)$ and isocaloric (14.5 MJ /kg diet) diets for 7 weeks. Survival of all groups were above $80\%$. Weight gain, feed efficiency and protein efficiency ratio were not significantly affected by dietary Sargassum meal levels (P>0.05). There were no significant differences (P>0.05) in moisture, crude protein, crude lipid, crude ash and fatty acid compositions of whole-body fish among groups. It is concluded that Sargassum meal could be used as a dietary additive or alternative low-cost dietary ingredient up to $10\%$ for juvenile ayu reared in seawater.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권6호
• /
• pp.795-801
• /
• 2005

Eighteen Suffolk wether lambs (BW = 24 kg) were chronically exposed to temperatures of cold (2$^{\circ}C$) or warm (22$^{\circ}C$). The experimental design consisted of a 2${\times}$3 factorial with a single crossover of environment treatment. The sheep were closely shorn and were housed in individual metabolic crates in controlled environment rooms. Sheep consumed pelleted diets ad libitum, which consisted of mainly barley grain and brome grass, and diets contained 7, 11 or 14% crude protein (CP). Animals were catheterized via one jugular vein with a PVC catheter and received a single injection of 60-65 Ci of $^{14}$C]urea. Plasma urea-N (PUN), urinary urea (UU), and carbon specific radioactivity were measured. Urea metabolism was not affected by environment. Percent urea recycling and urea space clearance were highest (p<0.05) on the low nitrogen diet. Urea pool was increased (p<0.10) for the 14% CP diet. Both UU and PUN concentration were positively related (p<0.01) with diet CP content. Therefore, dietary CP content significantly influenced urea metabolism, however, cold exposure did not alter those parameters.

• 한국작물학회지
• /
• 제48권5호
• /
• pp.372-375
• /
• 2003

In order to examine the possibility that oil seed rape could be used as a forage fodder crop and to select the most suitable variety of forage rape at the southern area of Korea, two varieties of oil seed rape currently grown for oil production and six introduced varieties of forage rape with relatively high yield and high nutritional value were grown at the same condition and their nutritional value were observed. Generally, rape was considered as a useful forage fodder crop with high content of crude protein and low contents of NDF, ADF, hemicellulose, cellulose and lignin. Differences in mean values of the above characters between two groups of rape were not statistically significant. Velox showed significantly higher content of crude protein and significantly lower contents of NDF, ADF, hemicellulose, cellulose and lignin compared with other varieties of forage rape. Rape was relatively high in IVDMD compared with other forage fodder crops, and forage rape was more or less higher in IVDMD and DDMM than oil seed rape. Velox was the highest in IVDMD and DDMM among the varieties of forage rape in this experiment.