• 제어로봇시스템학회논문지
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• 제14권8호
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• pp.727-732
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• 2008

In this paper, the state-space representation of generalized complimentary filter is proposed. Complementary filter has the suitable structure to merge information from sensors whose frequency regions are complementary. First, the basic concept and structure of complementary filter is introduced. And then the structure of the generalized filter and its state-space representation are proposed. The state-space representation of complementary filter is able to design the complementary filter by applying modern filtering techniques like Kalman filter and $H_{\infty}$ filter. To show the usability of the proposed state-space representation, the design of Inertial Navigation System(INS) vertical channel damping loop using Global Positioning System(GPS) is described. The proposed GPS/INS damping loop lends the structure of Baro/INS(Barometer/INS) vertical channel damping loop that is an application of complementary filter. GPS altitude error has the non-stationary statistics although GPS offers navigation information which is insensitive to time and place. Therefore, $H_{\infty}$ filtering technique is selected for adding robustness to the loop. First, the state-space representation of GPS/INS damping loop is acquired. And next the weighted $H_{\infty}$ norm proposed in order to suitably consider characteristics of sensor errors is used for getting filter gains. Simulation results show that the proposed filter provides better performance than the conventional vertical channel loop design schemes even when error statistics are unknown.

• BMB Reports
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• 제53권7호
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• pp.373-378
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• 2020

Phosphorylation of the signaling component by protein kinase often leads to a kinase cascade or feedback loop. 3-Phosphoinositide-dependent kinase 1 (PDK1) signaling pathway diverges into various kinases including Akt and p70 S6 kinase (p70S6k). However, the PDK1 feedback mechanism remains elusive. Here, we demonstrated that UNC-51-like kinase (ULK1), an autophagy initiator kinase downstream of mechanistic target of rapamycin (mTOR), directly phosphorylated PDK1 on serine 389 at the linker region. Furthermore, our data showed that this phosphorylation affected the kinase activity of PDK1 toward downstream substrates. These results suggest a possible negative feedback loop between PDK1 and ULK1.

• Journal of Microbiology
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• 제41권3호
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• pp.239-247
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• 2003

The LRV1-4 capsid protein possesses an endoribonuclease activity that is responsible for the single site-specific cleavage in the 5' untranslated region (UTR) of its own viral RNA genome and the formation of a conserved stem-loop structure (stem-loop IV) in the UTR is essential for the accurate RNA cleavage by the capsid protein. To delineate the nucleotide sequences, which are essential for the correct formation of the stem-loop structure for the accurate RNA cleavage by the viral capsid protein, a wildtype minimal RNA transcript (RNA 5' 249-342) and several synthetic RNA transcripts encoding point-mutations in the stem-loop region were generated in an in vitro transcription system, and used as substrates for the RNA cleavage assay and RNase mapping studies. When the RNA 5' 249-342 transcript was subjected to RNase T1 and A mapping studies, the results showed that the predicted RNA secondary structure in the stem-loop region using FOLD analysis only existed in the presence of Mg$\^$2＋/ ions, suggesting that the metal ion stabilizes the stem-loop structure of the substrate RNA in solution. When point-mutated RNA substrates were used in the RNA cleavage assay and RNase T1 mapping study, the specific nucleotide sequences in the stem-loop region were not required for the accurate RNA cleavage by the viral capsid protein, but the formation of a stem-loop like structure in a region (nucleotides from 267 to 287) stabilized by Mg$\^$2＋/ ions was critical for the accurate RNA cleavage. The RNase T1 mapping and EMSA studies revealed that the Ca$\^$2＋/ and Mn$\^$2＋/ ions, among the reagents tested, could change the mobility of the substrate RNA 5' 249-342 on a gel similarly to that of Mg$\^$2＋/ ions, but only Ca$\^$2＋/ ions identically showed the stabilizing effect of Mg$\^$2＋/ ions on the stem-loop structure, suggesting that binding of the metal ions (Mg$\^$2＋/ or Ca$\^$2＋/) onto the RNA substrate in solution causes change and stabilization of the RNA stem-loop structure, and only the substrate RNA with a rigid stem-loop structure in the essential region can be accurately cleaved by the LRV1-4 viral capsid protein.

• 대한화학회지
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• 제42권2호
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• pp.209-213
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• 1998

(η6-mesitylene) manganese (Ⅰ) tricarbonyl hexafluorophosphate[MTH-Mn (Ⅰ)]과 황산 이메틸, 피로탄산 이에틸, 과망간산 칼륨 따위 화학탐침들을 사용하여 Pseudomonas alcaligenes 5S rRNA의 고차원 구조를 분석하였다. 5S rRNA의 삼차구조에서 MTH-Mn (Ⅰ)이 강하게 절단하는 자리들은 a고리의 $G_{12}AUGG_{16}$, b-C 구역의 3'쪽 가닥, 즉$G_{51}AAGUGAAGC_{60}$, B-a구역의 $U_{65}-AGCG_{69}$, d고리의 5'쪽 가닥의 $G_{72}AUGG_{76}$ 연속부분 들이다. MTH-Mn(Ⅰ)과 그밖의 화학 탐침들을 사용하여 얻은 절단 양식들에서 우리는, MTH-Mn(Ⅰ)으로 강하게 절단되는 연속부분들이 $tRNA^{Phe}$의 L자 구조의 모서리 부분에서와 같은 주머니 구조를 이룰 것이며, 이러한 구조를 형성할 때 b-C구역과 d고리가 돌쩌귀 구실을 하는 것으로 추정한다.

• 대한기계학회논문집B
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• 제22권11호
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• pp.1571-1581
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• 1998

A flow visualization study was carried out for the branch pipe mixing flow in which a jet was issued normally to the fully developed pipe flow. An instantaneous laser tomographic method was used for cross flow Reynolds numbers based on the cross flow diameter D ranged $Re_{cf}=5.26{\times}10^3{\sim}1.13{\times}10^4$, diameter ratios d/D = 0.1 ~ 0.2 and velocity ratios R = 0.5 ~ 10. Oil mist with the size of about $10{\mu}m$ diameter was used for the scattering particle. The main purpose of this study was to reveal the physical mechanism and the structure of vortices formation with varying the velocity ratios and diameter ratios in the branch pipe flow. It was found that the physical mechanism and the structures of vortices formation were quite different depending on the velocity ratios. Particularly in the case of R < 1, the typical vortex shows single loop shape and that for the case of R > 1 depicts mushroom-like structure in the cross flow jet.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2136-2141
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• 2007

The Loop Heat Pipe(LHP) system uses capillary forces so as to pump the working fluid from heat acquisition to heat rejecting systems. The performance of the LHP systems depends mainly upon the operating performance of the wick structure. The capillary pressure increases with decreasing the pore size of the wick structure. By the way, the wick structure's permeability decreases with decreasing the pore size and the porosity. To obtain an ideal wick, the wick structure should possess several characteristics such as the small pore size, high porosity and chemical compatibility with working fluid. Sintered metal wicks have been mainly used as the capillary wick structure mounted in LHP because of the fact that the sintered metal wick has some advantages like convenient selection of wick material, smaller pore size and so on as well as high reliability. In this study, sintered metal wicks were developed to meet required several parameters to design the high performance LHP systems for obtaining even more effective cooling technologies.

• 천문학회보
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• 제36권2호
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• pp.89.1-89.1
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• 2011

We have investigated a coronal jet near the limb on 2010 June 27 by Hinode/X-Ray Telescope (XRT), EUV Imaging Spectrograph (EIS), Solar Optical Telescope (SOT), SDO/Atmospheric Imaging Assembly (AIA), and STEREO. From EUV (AIA and EIS) and soft X-ray (XRT) images we identify the erupting jet feature in cool and hot temperatures. It is noted that there was a small loop eruption in Ca II images of the SOT before the jet eruption. Using high temporal and multi wavelength AIA images, we found that the hot jet preceded its associated cool jet. The jet also shows helical-like structures during the rising period. According to the spectroscopic analysis, the jet structure changes from blue shift to red one with time, implying the helical structure of the jet. The STEREO observation, which enables us to observe this jet on the disk, shows that there was a dim loop associated with the jet. Comparing the observations from the AIA and STEREO, the dim loop corresponds to the jet structure which implies the heated loop. Considering that the structure of its associated active region seen in STEREO is similar to that in AIA observed 5 days before, we compared the jet morphology on the limb with the magnetic fields extrapolated from a HMI vector magnetogram observed on the disk. Interestingly, the comparison shows that the open field corresponds to the jet which is seen as the dim loop in STEREO. Our observations (XRT, SDO, SOT, and STEREO) are well consistent with the numerical simulation of the emerging flux reconnection model.

• Molecules and Cells
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• 제24권3호
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• pp.378-387
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• 2007

The Bcl-2 family of proteins interacts at the mitochondria to regulate apoptosis. However, the anti-apoptotic Bcl-2 and $Bcl-X_L$ are not completely localized to the mitochondria. In an attempt to generate Bcl-2 and $Bcl-X_L$ chimeras that are constitutively localized to the mitochondria, we substituted their C-terminal transmembrane tail or both the C-terminal transmembrane tail and the adjacent loop with the equivalent regions from Bak or Bax mutant (BaxS184V) as these regions determine the mitochondrial localization of Bak and Bax. The effects of these substitutions on subcellular localization and their activities were assessed following expression in HeLa and CHO K1 cells. The substitution of the C-terminal tail or the C-terminal tail and the adjacent loop of Bcl-2 with the equivalent regions from Bak or the Bax mutant resulted in its association with the mitochondria. This change in subcellular localization of Bcl-2 chimeras triggered cells to undergo apoptotic-like cell death. The localization of this Bcl-2 chimera to the mitochondria may be associated with the disruption of mitochondrial membrane potential. Unlike Bcl-2, the loop structure adjacent to the C-terminal tail in $Bcl-X_L$ is crucial for its localization. To localize the $Bcl-X_L$ chimeras to the mitochondria, the loop structure next to the C-terminal tail in $Bcl-X_L$ protein must remain intact and cannot be substituted by the loop from Bax or Bak. The chimeric $Bcl-X_L$ with both its C-terminal tail and the loop structure replaced by the equivalent regions of Bak or Bax mutant localized throughout the entire cytosol. The $Bcl-X_L$ chimeras that are targeted to the mitochondria and the wild type $Bcl-X_L$ provided same protection against cell death under several death inducing conditions.

• BMB Reports
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• 제35권5호
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• pp.498-507
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• 2002

A gene that encodes a thermostable protease, coined thermicin, has been isolated from Thermoanaerobacter yonseiensis that is expressed and characterized in E. coli.. In order to elucidate the molecular characteristics on thermostability of the enzyme, molecular modeling and mutagenesis technology were applied. In the modeling structure, the structural core, including the active site, was well conserved; whereas, the two loop regions were unique when compared to thermitase. The mutant enzyme with the small loop deleted (D190-I196), based on modeling structural information, showed identical enzyme activity. However, when the large loop was deleted (P233-P244), a little lower $K_m$ and even a lower kcat was found. This indicates that the large loop could influence catalytic activity. However, the unfolding temperature ($T_m$), which was determined by a differential-scanning calorimetry for the mutant enzyme deleted the small loop, was $96^{\circ}C$. This is $14^{\circ}C$ lower than that for the parent thermicin. These results suggest that the small loop may play a role in maintaining the proper folding of the enzyme at high temperatures, whereas the large loop might be related to catalysis.

• 한국전자파학회논문지
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• 제25권1호
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• pp.25-31
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• 2014

본 논문에서는 인체 내 외부간 근거리 통신망인 WBAN(Wireless Body Area Network)에서 인체 부착형 기기간 통신링크를 원활하게 구성하기 위한 변형된 Alford 안테나를 제안하였다. 제안된 안테나는 인체의 영향을 고려해 설계하였으며, ISM(The Industrial, Scientific and Medical, 2.4~2.485 GHz) 대역에서 동작한다. 기존 Alford 루프 안테나는 low-profile인 장점이 있지만, 인체 부착용 시스템의 다른 부품을 설치할 접지면이 존재하지 않고 balanced 급전 구조를 사용하기 때문에 on-body 안테나로 적합하지 않다. 따라서 본 논문에서는 on-body 환경에서 동작할 수 있는 접지면이 포함된 unbalanced 급전 구조를 갖는 안테나를 제안하였다. 또한, 안테나가 인체에 부착되어 안테나 성능에 미치는 영향을 고려하기 위해 모의 인체를 통해 모의실험과 측정을 수행하였다. 제안된 안테나는 모노폴 형태의 방사 패턴을 갖는 low-profile 구조이며, ISM 대역 내에서 10 dB 반사 손실을 만족한다. 안테나는 on-body 통신 환경에 적합한 표면지향성 특성을 갖는다.