• Natural Product Sciences
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• v.21 no.2
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• pp.128-133
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• 2015

Lindera obtusiloba has been widely used as a traditional medicine for the treatment of lots of diseases, including abdominal pain, bruise, and hepatocirrhosis. Here in this study, we elucidated the lifespan-extending effect of methanolic extract of Lindera obtusiloba (MLO) using Caenorhabditis elegans model system. We found that MLO has potent lifespan extension activities under normal culture condition. Then, we determined the protective effects of MLO on the stress conditions such as osmotic, thermal and oxidative stress. To reveal possible mechanism of MLO-mediated lifespan, we further investigated the effect of MLO on the antioxidant enzyme activities and intracellular ROS levels. Our results demonstrated that superoxide dismutase and catalase activities were significantly up-regulated by MLO treatment, resulted in reduced intracellular ROS levels. In this work, we also tested whether MLO-mediated longevity activity was associated with aging-related factors such as food intake and growth. Our data revealed that both of pharyngeal pumping rate and body length were significantly shifted by MLO treatment, indicating these factors were involved in MLO's lifespan-extension effects. Although MLO induces reduction in food intake, the body movement of MLO-fed aged worms was not decreased, compared to untreated control worms, indicating MLO might extend lifespan without affecting healthspan.

• Journal of Nutrition and Health
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• v.39 no.3
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• pp.286-298
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• 2006

The study aimed to identify major factors related to global self-rated health of the community-dwelling elderly people in rural areas. Interviews were conducted with 433 persons over 65 years of age. The socio-economic characteristics, chronic disease status, measures of functional and mental health, life satisfaction, health-related behaviors including smoking, drinking, exercise, social activity, dietary habit, and food intakes were analyzed to determine their influence on self-rated health index. Data on food intake were obtained through the 24-hour recall method. The self-rated health of rural elderly was poor or very poor as reported by 42.6% and 52.4% of the men and women, respectively. Poor self-rated health was found to be related to elderly aged $65{\sim}74$, female, absence of work, more chronic diseases, dependence on Instrumental Activity Daily Living (IADL), higher mental unhealthy days, lower current life satisfaction, less social activity, lower dietary habit scores, lower intake of total food, fruit, eggs, fishes & shell fishes, vitamin C (%RDA), and Nutrient Adequacy Ratio (NAR). The results of the multiple regression analysis showed that poor self-rated health index is significantly associated with more chronic diseases, mental unhealthy days, gastrointestinal disease, musculoskeletal disease, less social activity, and lower intake of fruits. The results also suggested that improving the nutritional status and functional ability, and reducing the burden of chronic diseases are beneficial to the self-rated health index of the elderly.

• Biomolecules & Therapeutics
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• v.23 no.6
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• pp.582-589
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• 2015

Several theories emphasize that aging is closely related to oxidative stress and disease. The formation of excess ROS can lead to DNA damage and the acceleration of aging. Vigna angularis is one of the important medicinal plants in Korea. We isolated vitexin from V. angularis and elucidated the lifespan-extending effect of vitexin using the Caenorhabditis elegans model system. Vitexin showed potent lifespan extensive activity and it elevated the survival rates of nematodes against the stressful environments including heat and oxidative conditions. In addition, our results showed that vitexin was able to elevate antioxidant enzyme activities of worms and reduce intracellular ROS accumulation in a dose-dependent manner. These studies demonstrated that the increased stress tolerance of vitexin-mediated nematode could be attributed to increased expressions of stress resistance proteins such as superoxide dismutase (SOD-3) and heat shock protein (HSP-16.2). In this work, we also studied whether vitexin-mediated longevity activity was associated with aging-related factors such as progeny, food intake, growth and movement. The data revealed that these factors were not affected by vitexin treatment except movement. Vitexin treatment improved the body movement of aged nematode, suggesting vitexin affects healthspan as well as lifespan of nematode. These results suggest that vitexin might be a probable candidate which could extend the human lifespan.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.11-15
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• 2002

The present investigation was undertaken to study the effects of non-genetic factors and association among replacement and lifetime production traits. The data on 542 Holstein Friesian cows maintained during 1975-98 at State Cattle Breeding Project, Sector III, Hisar, were utilized. The average sex-ratio, abnormal births, mortality, culling and replacement rates on total calf born and total female calf born basis were 51.62, 8.50, 17.52, 31.05, 22.78 and 51.41 per cent, respectively. The study revealed that a minimum of 4 to 5 progenies are required per cow over its lifetime to replace itself. It indicated that each cow should produce a minimum of 2 female calves during its life so as to replace herself before being lost. The least-squares means for productive herd life, longevity and lifetime production were $1439.32{\pm}87.64$ and $2419.18{\pm}8.25$ days and $11317.95{\pm}913.15kg$, respectively. The heritability estimates for all replacement traits were very low indicating that sire selection may bring no desirable change in these traits. Heritability estimates were $0.178{\pm}0.157$, $0.288{\pm}0.184$ and $0.096{\pm}0.195$ for corresponding lifetime production traits. Breeding values and ranking of sires were generated for replacement and lifetime production traits to estimate the rank correlations between these traits. Moderate desirable rank correlations were obtained between replacement rate and lifetime production traits indicating that sires proven on the basis of milk production are also expected to have better replacement rate.

• Korean Journal of Pharmacognosy
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• v.46 no.1
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• pp.38-43
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• 2015

Ligularia stenocephala has a wide range of types of constituents with various pharmacological properties. Here in this study, we examined the effect of methanolic extract of L. stenocephala (MLS) on the lifespan and stress tolerance using Caenorhabditis elegans model system. We found that lifespan of wild-type worms was significantly lengthened in the presence of MLS in a dose dependent manner. MLS also elevated the tolerance of worms against osmotic, heat shock, and oxidative stress. We also demonstrated in vivo antioxidant capacity of MLS by checking intracellular reactive oxygen species levels as well as antioxidant enzyme activities such as catalase and superoxide dismutase. We further investigated several aging-related factors, including pharyngeal pumping rate and body length. Here, we showed that MLS exerts longevity effect independent of both factors. In addition, body movement of aged worms was significantly elevated, suggesting MLS could enhance healthspan as well as lifespan.

• Journal of Society of Preventive Korean Medicine
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• v.17 no.3
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• pp.1-18
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• 2013

Objectives: Korea has a dual medical system where traditional Korean Medicine (KM) and Western Medicine (WM) exist au equal terms with exclusive practice boundaries. The aim of this study was to identify complementary and substitute relationships between KM and WM in Korea. Methods: The data of 19,413 respondents were collected from the 2009 Korea Health Panel dataset. General characteristics and the medical utilization of respondents were analyzed descriptively. the Univariable Analysis was used to compare the factors that affected KM and WM utilization, and the Multivariable Analysis was applied to identify complementary or substitute relationships between the respondents' choices for KM and WM. The data were analyzed by the seven disease groups; diseases of nervous system, circulatory system, respiratory system, digestive system, skin and subcutaneous tissue, musculoskeletal system, and connective tissue, injury, poisoning and others. Results: 13.6% and 76.9% of respondents used KM and WM respectively last 12 mouths. 12.7% used both, and 0.9% used KM only. In overall, respondents who visited KM institutions used also WM. However, according to the analysis of choices of medical institutions, non-pharmacological KM treatment and WM has been used as a substitute for another in the diseases of the skin and subcutaneous tissue, diseases of the musculoskeletal system, and connective tissue, injury, poisoning and others. Conclusions: Despite some exceptional disease areas, Korean people use KM complementarily to WM, and this result can rationalize the recent Korean government policies encouraging the cooperation of KM and WM. This study can he used for the future policies development for KM service delivery.

• Journal of Pharmacopuncture
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• v.18 no.4
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• pp.20-25
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• 2015

Objectives: Recently, thread-embedding therapy (TET) has been widely applied in Korean medicine for cosmetic purposes such as reducing skin wrinkles. An inserted thread was reported to have induced continuous stimulation, followed by support for connective tissue regeneration. However, the potential role of TET in hair-growth has not yet been reported. Methods: We designed this study to evaluate whether TET has a hair-growth-promoting effect. C57 black 6 (C57BL/6) mice were divided into three groups: normal saline-treated, minoxidil-treated, and thread-embedded groups. Normal saline or 5% minoxidil was topically sprayed on the dorsal skin of the mice once a day for 16 days. Medical threads were embedded into the dorsal skin of the mice in a single application. Hair growth activity was evaluated by using dermoscopic and microscopic observations. Sections of the dorsal skin were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA), fibroblast growth factor-7 (FGF-7), and fibroblast growth factor-5 (FGF-5) were detected by using immunohistochemical staining. A reverse transcription-polymerase chain reaction (RT-PCR) analysis was adopted to measure the messenger RNA (mRNA) expressions of FGF-7 and FGF-5. Results: TET enhanced anagen development in the hair follicles of C57BL/6 mice. The expressions of BrdU and PCNA, both of which imply active cellular proliferation, were increased by using TET. Moreover, TET increased the expression of FGF-7, an anagen-inducing growth factor, while decreasing the expression of FGF-5, an anagen-cessation growth factor, both at the protein and the mRNA levels. Conclusion: TET enhanced hair re-growth in C57BL/6 mice. TET regulated the expressions of anagen-associated growth factors and activated the proliferation of hair follicular cells in depilated skin lesions. Considering its long-lasting effect, TET may be a good alternative therapeutic for the treatment of alopecia.

• Journal of Society of Preventive Korean Medicine
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• v.17 no.2
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• pp.47-68
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• 2013

Objectives : This research aimed to develop a new etiologic classification for traditional Korean Medicine in order to respond to the social and environmental change. Methods : We reviewed the existing theories on etiological classification for East Asian Medicine thoroughly and discussed the problems and limitations. Based on the experts' consensus, we abstracted disease factors and etiologic items. Results : The disease factors are classified into three parts: the human body, the environment, and the interaction between the human body and the environment. We defined them as the inner factor, the external factor, and the interaction between the inner and the external factors. The inner factor is free from the influence of the environment, and it causes diseases solely from the components of the human body. It is divided into genetic factors. The external factor is defined as a case when a disease occurs due to a factor outside the human body and includes external injuries, environmental pollution, and natural disasters. The interaction between the inner and the external factors is a disease factor that causes diseases by the interaction of the human body and the environment and includes emotions, habits, and social environment. As a result of the analysis, it was possible to see the meanings at a single glance as the scattered and fractional meanings were integrated with focus on medicinal herbs, but the increasing number of analyzed medicinal herbs tended to more and more complicate their relationships, thus, requiring additional work like filtering. Conclusions : The new etiologic classification of Korean Medicine fully reflects the perspectives on life in Korean Medicine while embracing the changes in modem society. Also, by avoiding the usage of ambivalent terms and wrong classification methods, the new classification system constructs intuitive and concise etiology and improves usability in clinical medicine.

• Korean Journal of Family Social Work
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• no.57
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• pp.159-182
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• 2017

With using the 4th and 5th wave of KLoSA(Korean Longitudinal Study of Aging), this study analyzed the effect of the factors of Rowe and Kahn's successful aging model on the life satisfaction of older adults. The target population of this study was 1,385 who is older than 65. As data analysis, hierarchical multiple regression was used. The variables such as demographics, avoiding diseases and disability, high cognitive and physical function and engagement with life, relationship with adult children were entered hierarchically for the regression analysis. Since there are differences of longevity by gender, the difference of factors of successful aging were compared by marital status. In this study (besides Rowe and Kahn's successful aging model such as avoiding disease and disability, high cognitive and physical function, and engagement with life), the relationships with adult children were analyzed additionally. Findings show that married older adults were older and reside with their adult children twice more than those who do not have a spouse. According to research results, regular exercise and memory skills were important factors of affecting life satisfaction. Among the categories of active engagement, meeting with friends, relatives and neighbors were significant. Contact with their adult children by mail or phone were the most important factors affecting the older adults' life satisfaction. The three parts of successful aging were various depending on the operationalization of variables.

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.484-493
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• 1982

This study was conducted to clarify the effects of daminozide (butanedioic acid mono-(2,2 - dimethylhydrazide)) sprayed 2 days be fore harvest and floral preservative solutions on longevity, quality and ethylene production of 'Georgia' cut lily for prolonging vase life and improving quality of cut lily flowers. And also the relation between ethylene production and flower senescence of cut lily was studied. 1. Lilies held in silver nitrate ($AgNO_3$) at 25 and 50 ppm were increased in solution uptake, fresh weight, and flower longevity. The solution of 2.5% sucrose+50 ppm $AgNO_3$+200 ppm aluminum sulfate+10 ppm 6-benzylamino purine prolonged vase life and improved quality of cut lily flowers. 2. Lilies sprayed with 500 ppm daminozide 2 days be fore harvest and then held in the preservative solution (5% sucrose+50 ppm $AgNO_3$+150ppm 8-hydroxyquinoline) after harvest were significantly increased in fresh weight and vase life as compared with non-sprayed (control) flowers. 3. Ethylene production from cut lily was increased by ethephon ((2-chloroethyl) phosphonic acid) treatment. The flowers producing a lot of ethylene, however, were senesced slowly instead of rapid wilting. 4. The preservative solution markedly reduced ethylene production of cut lily but prolonged vase life for only a few days. 5. These results suggest that ethylene be one of the most important factors promoting flower senescence of cut lily, but it be very difficult to prolong vase life remarkably by only inhibition of ethylene production in cut lily.