• Title/Summary/Keyword: Long-rod

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Isolation and Characterization of $\alpha$-Amylase Producing Bacillus sp. AIV 1940 and Properties of Starch Synthetic Wastewater Degradation ($\alpha$-Amylase 생성균주 Bacillus sp. AIV 1940의 분리, 특성 및 합성폐수분해능)

  • 박형수;김무훈;양선영;조미영;고범준;박용근
    • Korean Journal of Microbiology
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    • v.38 no.1
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    • pp.1-6
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    • 2002
  • $\alpha$-Amylase producing bacteria were isolated from activated sludge of corn processing wastewater plant and paddy field soil samples and selected by the direct iodine reaction. The isolate was identified as Bacillus sp. after morphology, API system and fatty acid analyses. To enchance $\alpha$-amylase productivity, a successive mutation of Bacillus sp. AIV 19 was performed using the treatment of nitrosoguanidine(NTG).The mutant, Bacillus sp. AIV 1940, showed about 1.8-fold level of amylase activity compared with parental strain. The isolate was Gram-positive and rod (2.8-3.0 $\mu$m long, 0.5-0.6 $\mu$m wide) type. The strain increased the bacterial mass at 3000 mg/l starch concentration. Organic substance removal rate was 40.2, 72.3% respectively after 1 and 3 day reaction using starch synthetic wastewater (intial CODcr was 4,455 mg/l).

Comparison of cone beam CT and conventional CT in absorbed and effective dose (Cone beam CT와 일반 CT의 흡수선량 및 유효선량 비교평가)

  • Kim, Sang-Yeon;Han, Jin-Woo;Park, In-Woo
    • Imaging Science in Dentistry
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    • v.38 no.1
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    • pp.7-15
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    • 2008
  • Purpose: This study provides comparative measurements of absorbed and effective doses for newly developed cone beam computed tomography (CT) in comparison with these doses for conventional CT. Materials and Methods: Thermoluminescent dosimeter rods (TLD rod: GR-200, Thermo Fisher Scientific Inc., Waltham, MA, USA) were placed at 25 sites throughout the layers of Male ART Head and Neck Phantom (Radiology Support Devices Inc., Long Beach, USA) for dosimetry. Implagraphy, DCT Pro (Vatech Co., Hwasung, Korea) units, SCT-6800TXL (Shimadzu Corp., Kyoto, Japan), and Crane x 3+(Soredex Orion Corp., Helsinki, Finland) were used for radiation exposures. Absorption doses were measured with Harshaw 3500TLD reader (Thermo Fisher Scientific Inc., Waltham, MA, USA). Radiation weighted doses and effective doses were measured and calculated by 2005 ICRP tissue weighting factors. Results: Absorbed doses in Rt. submandibular gland were 110.57 mGy for SCT 6800TXL (Implant), 24.56 mGy for SCT 6800TXL (3D), 22.39 mGy for Implagraphy 3, 7.19 mGy for DCT Pro, 5.96 mGy for Implagraphy 1, 0.70 mGy for Cranex 3+. Effective doses $(E_{2005draft)$ were 2.551 mSv for SCT 6800TXL (Implant), 1.272 mSv for SCT 6800TXL (3D), 0.598 mSv for Implagraphy 3, 0.428 mSv for DCT Pro and 0.146 mSv for Implagraphy 1. These are 108.6, 54.1, 25.5, 18.2 and 6.2 times greater than panoramic examination (Cranex 3+) doses (0.023mSv). Conclusion: Cone beam CT machines recently developed in Korea, showed lower effective doses than conventional CT. Cone beam CT provides a lower dose and cost alternative to conventional CT, promising to revolutionize the practice of oral and maxillofacial radiology.

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Isolation and Culture Conditions of Hydrogen Producing Bacterium Enterobacter sp. ES392 (수소생산균 Enterobacter sp. ES392의 분리 및 배양조건)

  • Jeon, Sung-Jong;Lee, Eon-Seok
    • Microbiology and Biotechnology Letters
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    • v.38 no.4
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    • pp.399-404
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    • 2010
  • A hydrogen-producing bacterium (strain ES392) was isolated from pond water located in the Dong-Eui University, Busan, Korea. The cell was long-rod type ($1.4\;{\mu}m$) of about ($0.6\;{\mu}m$) in diameter, and not formed flagellum and spore. Phylogenetic analysis based on the 16S rRNA sequence and biochemical studies indicated that ES392 belonged to the genus Enterobacter sp. The optimum pH and temperature for hydrogen production was 7.5 and $35^{\circ}C$, respectively. The optimization of medium compositions which maximize hydrogen production from Enterobacter sp. ES392 was determined. As a result, the maximum hydrogen production was obtained under the conditions of 4% (w/v) sucrose, 0.5% (w/v) yeast extract and 50 mM potassium phosphate buffer (pH 7.5). Under batch culture conditions, the maximal hydrogen production and yield were obtained as 3481 mL/L and 1.33 mol/mol sucrose, respectively.

Fabrication of Microwave PECVD with Linear Antenna for large-scale deposition processing, and Analysis of Ar plasma characteristics using Electrostatic Probe and Temperature Characteristics (대면적 증착용 선형 초고주파 플라즈마 장치 제작 및 정전 탐침법을 이용한 Ar 플라즈마 특성 분석과 온도 특성 분석)

  • Han, Moon-Ki;Seo, Kwon-Sang;Kim, Dong-Hyun;Lee, Ho-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.64 no.3
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    • pp.422-428
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    • 2015
  • A 2.45GHz microwave plasma source with a linear antenna has been developed for low temperature large scale deposition processing. Microwave power is transmitted through WR340 waveguide and a copper rod, linear antenna, is located in a quartz tube. The power matching is effectively achieved by a linear antenna is located at ${\lambda}_g/4$ or $3{\lambda}_g/4$ from the end of WR340 waveguide. The Ar plasma was generated along the surface of quartz tube and a clear standing wave pattern with nearly 10cm wavelength was observed at Ar pressure of 200mTorr and 200W input power. The electron density and electron temperature were investigated by using the electrostatic probe. The electron density and electron temperature were highly measured near the surface of quartz tube. Ar plasma density along the quartz tube is mostly uniform despite standing wave set-up and antenna of long length. A uniform temperature was measured at 10~40cm distance from the end quartz tube and 5cm distance from the surface of quartz tube.

Definition of the Earth-volume in Breakwater Using Real-time DGPS & Echo-Sounding data (실시간 DGPS & Echo-Sounding 데이터를 이용한 방파제사석투하 토공물량 확인)

  • 서용운;최윤수
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.18 no.4
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    • pp.343-350
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    • 2000
  • This study deals with the Definition of the Earth-volume quantity and direction of throwing down stones (Q.R.R) into the Breakwater using Real-time DGPS & Echo-Sounding method. Generally, the buoy in the center line of Breakwater has been set up so as to throw down stones in the correct area. After throwing stones down there, surveyors have been surveying the depth and direction of stones with rod and sounding lead. The method, however, is not effective because of long time and a lot of human power, in addition it is incorrect. This paper has studied on the solution of those problems using Real-time DGPS & Echo-Sounding data to calculate the earth-volume quantity, direction and depth of throwing down stones. This paper says the effective and economical methods using Real-time DGPS & Echo-Sounding data there.

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Draft genome sequence of lytic bacteriophage KP1 infecting bacterial pathogen Klebsiella pneumoniae (병원균 Klebsiella pneumoniae를 감염시키는 용균 박테리오파지 KP1의 유전체 염기서열 초안)

  • Kim, Youngju;Bang, Ina;Yeon, Young Eun;Park, Joon Young;Han, Beom Ku;Kim, Hyunil;Kim, Donghyuk
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.152-154
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    • 2018
  • Klebsiella pneumoniae is a Gram-negative, rod-shape bacterium causing disease in human and animal lungs. K. pneumoniae has been often found to gain antimicrobial resistance, thus it has been difficult to treat K. pneumoniae infection with antibiotics. For such infection, bacteriophage can provide an alternative approach for pathogenic bacterial infection with antimicrobial resistance, because of its sensitivity and specificity to the host bacteria. Bacteriophage KP1 was isolated in sewage and showed specific infectivity to K. pneumoniae. Here, we report the draft genome sequence of Klebsiella pneumoniae phage KP1. The draft genome of KP1 is 167,989 bp long, and the G + C content is 39.6%. The genome has 295 predicted ORFs and 14 tRNA genes. In addition, it encodes various enzymes which involve in lysis of the host cell such as lysozyme and holin.

Evaluation of Aging Degradation in 2.25Cr-1Mo Steel by Coercivity and Remanence Measurements - Microstructural Approach (보자력 및 잔류자화를 이용한 2.25Cr-1Mo강의 경년열화도 평가 - 미세조직적 접근)

  • Byeon, Jai-Won;Kwun, Sook-In
    • Journal of the Korean Society for Nondestructive Testing
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    • v.22 no.1
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    • pp.65-73
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    • 2002
  • Artificial aging was performed to simulate the microstructural degradation in 2.25Cr-1Mo steel arising from long time exposure at $540^{\circ}C$. Microstructural analysis (mean equivalent size, number of carbides per unit area) and measurement of mechanical properties(UTS, Vickers hardness) and magnetic properties(coercivity, remanence) were performed. By comparing these results, the relationship between magnetic properties and microstructural changes with artificial aging was clarified. The carbides were classified as rod, globular and acicular type in terms of morphology. The fine acicular carbides were found to diminish drastically in the initial stage of aging. The magnetic coercivity and remanence were observed to decrease rapidly in the initial about 920 hours of aging time and then decrease slowly afterwards. Linear correlations between the mechanical properties and magnetic properties such as correlations remanence were found.

Isolation and Characterization of a New Cellulase-producing Marine Bacterium, Seonamhaeicola sp. S2-3 (셀룰로스분해 신규 해양미생물 Seonamhaeicola sp. S2-3의 분리 및 동정)

  • Kim, Da Som;Chi, Won-Jae
    • Microbiology and Biotechnology Letters
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    • v.48 no.4
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    • pp.539-546
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    • 2020
  • A cellulolytic bacterial strain, S2-3, was isolated from sea water collected in Jeju island, Republic of Korea. The strain was aerobic and gram negative, and formed yellow colored colonies on marine agar medium. S2-3 cells were long rod-shaped, 0.5 × 0.25 ㎛ (width x length) in size, and did not have flagella. The optimal growth conditions for S2-3 were 30-35℃ and pH 6.5-7.0. Analysis of the 16S rRNA gene sequence of S2-3 revealed that it had the highest identity with those of Seonamhaeicola algicola Gy8 (97.08%), Hyunsoonleella udonensis JG48 (95.01%), and Aestuariibaculum scopimerae I-15 (94.86%). In phylogenetic analysis, S2-3 formed the same clade as S. algicola Gy8, implying that S2-3 belongs to the genus Seonamhaeicola. The major fatty acids (>10%) comprised C15:1 iso G (22.29%), C15:0 iso (17.71%), C17:0 iso 3OH (16.06%), and C15:0 iso 3OH (10.7%), resulting in quite different ratio of the component from those of S. algicola Gy8. Moreover, its biochemical characteristics, including acid production and enzyme activities, were different from those of S. algicola Gy8. Therefore, putting all these results together, we concluded S2-3 is distinct species from S. algicola Gy8, and thus named it Seonamhaeicola sp. S2-3. In liquid culture, S2-3 produced extracellular cellulases that can hydrolyze cellulose or cellooligosaccharides into cellobiose, which is a good enzyme resource that deserves further research.

Research on no coal pillar protection technology in a double lane with pre-set isolation wall

  • Liu, Hui;Li, Xuelong;Gao Xin;Long, Kun;Chen, Peng
    • Geomechanics and Engineering
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    • v.27 no.6
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    • pp.537-550
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    • 2021
  • There are various technical problems need to be solved in the construction process of pre-setting an isolation wall into a double lane in the outburst prone mine. This study presents a methodology that pre-setting an isolation wall into a double lane without a coal pillar. This requires the excavation of two small section roadways to dig a wide section roadway, followed by construction of the separation wall. During this process the connecting lane is reserved. In order to ensure the stability of the separation wall, the required bearing capacity of the isolation wall is 4.66 MN/m and the deformation of the isolation wall is approximately 25 cm. To reduce the difficulty of implementing support the roadway is driven by 5 m/d. After the construction of the separation wall, the left side coal wall is brushed 1.5 m to make the width of the gas roadway reach 2.5 m and the roadway support utilizes anchor rod, ladder beam, anchor cable beam and net configuration. During construction, the concrete pump and removable self-propelled hydraulic wall mold are used to pump and pour the concrete of the isolation wall. In the process of mining, the stress distribution of coal body and isolation wall is detected and measured on site. The results demonstrate that the deformation of the surrounding rock of roadway and separation of roof in the roadway is small. The stress of the bolt and anchor cable is within equipment tolerance validating their selection. The roadway is well supported and the intended goal is achieved. The methodology can be used for reference for similar mine gas control.

A Study on the Construction Status and the Structural System Features of Wooden Large Space Buildings (대공간 목구조 건축의 건립 현황과 구조시스템 특성 분석)

  • Lee, Juna;Lee, Hyunghoon;Lee, Seong-Jae
    • Journal of Korean Association for Spatial Structures
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    • v.22 no.3
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    • pp.15-24
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    • 2022
  • In this research, the case of modern wooden structures since 1950 with span of 30m or more was investigated and analyzed the construction status and structural planning characteristics of wooden large space architecture. As a result, wooden large space buildings have built around Asia, North America, and Europe, in which cases of ice skating stadiums with span of 30m to 60m were concentrated. In the case of baseball parks and football stadiums, even a span of about 165m was built in a wooden structure. In addition, it was found that the structural systems used in wooden large space structures were a funicular arch and truss structure, in that cases, funicular arch system consisting of radial arrangements was used in the examples exceeded 150m and the two way truss system was also used in long span wooden structures exceeding 100m. As the truss structure with a tie-rod or the flexure+tension structure was partially investigated, it can be seen that various timber structural systems need to be devised and researched. Also, It was investigated that a technique in which some members of the truss are made of steel or a composite member of steel and timber is also possible to develop