• Title/Summary/Keyword: Long line culture

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THE GROWTH OF FOOD ORGANISMS FOR THE MASS PRODUCTION OF MOLLUSCAN SEEDLINGS 1. Phaeodactylum, Platymonas and Chlorella (패류 종묘의 대 생산시에 필요한 먹이 생물의 배양에 관한 연구 1. Phaeodactylum, Platymonas 및 Chlorella)

  • Yoo Sung Kyoo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.1
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    • pp.1-6
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    • 1970
  • A series of experiments have been made on the mass culture of Phaeodactylum tricornutum, Platymonas sp. and Chlorella sp. in the laboratory. The shortest lag phase was found In the culture of Ph. tricornutum followed by Platymonas sp. and Chlorella sp. As compared to the aeration culture, the stagnant culture, in general, showed long duration of the lag phase, short period of the exponential phase and extremely small daily increment. The relative growth constants of Ph. tricornutum, Chlorella sp. and Platymonas sp. were $0.302{\pm}0.028$, $0.226{\pm}0.013$, and $0.151{\pm}0.008$, respectively The maximum daily increment of the three species and then daily ages are as follows: Ph. tricornutum Maximum daily increment : 47.5, Daily age : 10, Platymonas sp. Maximum daily increment : 5.6, Daily age : 14, Chlorella sp. Maximum daily increment : 21.1, Daily age : 14 Comparing the packed cell volume with a certain number of cells, the largest value was found in the population of Ph. tricornutum followed by Platymenas sp. and Chlorella sp. A straight line relationship exists between the two values, and the magnitude of the relationship coincides well with the size of the cells. The culture of Ph. tricornutum was proved satisfactory for feeding the larvae of bivalves at about 12 days after innoculation and both of Platymenas sp. and Chlorella sp. were about 16 days respectively.

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Study on the modern design application of traditional formative beauty of Korea - Focused on the formative characteristics of lines and patterns observed in the necessities of Joseon society - (한국 전통 조형미의 현대 디자인 적용에 관한 연구 - 조선 사회의 실용품에 나타나는 선(線)과 문양(文樣)의 조형적 특성을 중심으로 -)

  • Jung, Min-hee;Kim, So-hyung;Yoon, Se-hwan
    • Journal of Communication Design
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    • v.59
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    • pp.298-308
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    • 2017
  • In the modern society in which globalization is ongoing in the overall culture social culture, various efforts are being made by each country around the world in order to search for the distinctive cultural identity and creativity. Recognizing the importance of the 'Korean' tradition, South Korea is also actively engaged in researches to develop design utilizing it and to apply it in real life. Here, developing Korean design does not simply refer to borrowing and imitating the traditional form or color but refers to generating a new, original and ethnic aesthetic consciousness that conform to the era that we are currently living in based on the unique ideology and sentiment inherent in its formativeness. Despite the fact that research on Korean traditional culture has continued for a long period of time, it is considered that such phenomenon continues to take place because the establishment and utilization of Korean culture identity is still insufficient. There, research on various fields based on new understanding with regards to our culture and the development of design utilizing it is in dire need. This study aims to analyze the said usage form and formative characteristics and the possibility of modern application focused on the lines and patterns which are most frequently mentioned when discussing about the Korean traditional formative beauty of Korea.

Exploring the Nature of Caring in Hwa-Byung - Transpersonal Caring-Healing Model - (화병에서의 돌봄의 본질 -Transpersonal Caring-Healing Model을 중심으로-)

  • Khim Soon-Yong;Lee Cho-Sik;Kong Byung-Hye;Shin Joo-Young
    • Journal of Korean Academy of Nursing
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    • v.35 no.2
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    • pp.225-238
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    • 2005
  • Hwa-Byung(HB) has been categorized as a Korean culture-bound syndrome that is prevalent in married women of low socioeconomic status. Purpose: The Purpose of the study was to search for the essence of HB and a caring-healing process of HB. Then the research result discusses whether the Transpersonal Caring-Healing Model has been congruent with it. Method: Case examples resulted from in-depth telephone counseling over a period of time at the Women's Hot Line with a client who is a housewife with HB. The counseling content was analyzed through Giorgi's method of descriptive phenomenology. Result: The core meaning of the essence of HB was 'injustice'; and essential themes were 'lack of reciprocity', 'infidelity' 'suppressed aggression and powerlessness' and 'need for recognition'. The core meaning of the essence of the caring-healing process was 'caring-healing experience(maintaining a trust relationship)'; and essential themes were 'active listening', 'empathy' and 'forming a therapeutic relationship'. According to Watson, 'active listening', 'empathy' and 'forming a therapeutic relationship' were identified as intentionality, intersubjectivity and transpersonal. Conclusion: Transpersonal caring can release inner power and strength and help the person to gain a sense of inner harmony. Transpersonal caring is as important to healing as are conventional treatment approaches and even more powerful in the long run.

Bioassays of Polycyclic Aromatic Hydrocarbons in ZR-75-1 Human Breast Cancer Cells

  • Min, Kyung-N.;Sheen, Yhun-Y.
    • Environmental Mutagens and Carcinogens
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    • v.23 no.1
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    • pp.35-40
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    • 2003
  • Polycyclic aromatic hydrocarbons (PAH) are frequently detected in food, water, soil, and sediment and are widespread environmental pollutants formed by the incomplete combustion of fossil fuels, woods and other organic matter. PAHs are considered to be probable human carcinogens. The mechanism of action of PAHs has been studied extensively, however it is not clear how PAHs turn on CYP1A1 in human breast cancer. Our laboratory have been studied the effect of PAHs in the human breast cancer cell MCF7. In this study, we examined the ZR-75-1 human breast cancer cells as a new system to evaluate bioactivity of PAHs. ZR-75-1 human breast cancer cell line responses to estrogen and progesteron. We have been able to estbilish long term culture system of this cells then used for the study to observe the effect of PAHs. We demonstrate that PAHs induced the CYP1A1 promoter and 7-ethoxyresolufin O-deethylase (EROD) activity in a concentration-dependant manner. RT-PCR analysis indicated that PAHs significantly up-regulate the level of CYP1A1 mRNA. Some of PAHs showed stronger stimulatory effect on CYP1 gene expression than TCDD. Apparently, ZR-75-1 cells have Aryl hydrocarbon receptors, therefore it would be good experimental tool to study the cross-talk between PAHs and steroid actions.

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The Study of Modernization of Korean Traditional Small Table (한국 전통 소반의 현대적 디자인 연구)

  • Baik, Eun
    • Journal of the Korea Furniture Society
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    • v.27 no.3
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    • pp.211-219
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    • 2016
  • Culture and design change with time and what they have in common is that both develop in line with the era's living environments. Today, various life styles have emerged to cause changes in housing and living modes and we are in need of designs that can be commonly used in any place. Under the circumstances, furniture plays a role of medium that helps interactions between the human and the space and it has long been used in a variety of ways amid the change of the times. Soban, Korean traditional furniture, is a tool used in our real life and it is a piece of furniture that caters for both arts and functionality. It has been more of a living necessity and accordingly carried wisdom of experience for survival, rather than artificial beauty. Unlike other types of furniture, it is a practical tool designed in consideration of users. Therefore, this study aims to find traditional furniture's wisdom of experience in Soban, which tends to get neglected, and ensure its value as a modern design element, and to contribute to express and study the formative arts of Korean design so that a past living necessity of Soban can overcome its limitation of being floor-seating furniture and become more than an eating tool in it value.

Study on the Pro-apoptotic Effect of Artemisiae Capillaris Herba Extracted with Ethanol on Biliary Tract Cancer Cell Line, SNU-1196 (인진(茵蔯)의 에탄올 추출물이 담도암 세포주 SNU-1196의 apoptosis에 미치는 효과에 관한 연구)

  • Lee, Kyung-Wook;Woo, Hong-Jung
    • The Journal of Internal Korean Medicine
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    • v.33 no.4
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    • pp.587-598
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    • 2012
  • Objectives : This study was performed to elucidate the pro-apoptotic effect of Artemisiae capillaris herba extracted with ethanol on biliary tract cancer cells. Materials and Methods : The biliary tract cancer cell line SNU-1196 was used in this study. Cells were treated with different concentrations of Artemisiae capillaris herba for 24, 48 and 72 hours. After the treatment, cell viability, apoptosis, caspase activities and the mRNA expressions of the Bcl-2, Bax, P53, and P21 were measured by using MTT assay, cell cycle analysis, apoptosis assay, and RT-PCR. The cell cycle analysis was done by flow cytometry and apoptosis assay by cell death detection ELISA kit. Results : Artemisiae capillaris herba inhibited proliferation of SNU-1196 in long-time culture group with dose-dependent manner. All cells treated with Artemisiae capillaris herba showed increased apoptosis with dose- and time-dependent manner. Exposure of SNU-1196 to Artemisiae capillaris herba induced caspase-3 activation. However, apeoptosis was blocked when SNU-1196 was treated together with the pan-caspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Z-DEVE-FMK. After the treatment of Artemisiae capillaris herba, the mRNA expressions of caspase -3, -8, -9, p53, and p21 was increased in all cells. Artemisiae capillaris herba resulted in a significant decrease in Bcl-2 and an increase in Bax mRNA levels. Conclusions : These results suggest that Artemisiae capillaris herba would be beneficial in the treatment of biliary tract cancer.

Anti-Proliferative Effects of Dendrophthoe pentandra Methanol Extract on BCR/ABL-Positive and Imatinib-Resistant Leukemia Cell Lines

  • Zamani, Afiqah;Jusoh, Siti Asmaa Mat;Al-Jamal, Hamid Ali Nagi;Sul'ain, Mohd Dasuki;Johan, Muhammad Farid
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.11
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    • pp.4857-4861
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    • 2016
  • Background: Imatinib mesylate, a tyrosine kinase inhibitor specifically targeting the BCR/ABL fusion protein, induces hematological remission in patients with chronic myeloid leukemia (CML). However, the majority of CML patients treated with imatinib develop resistance with prolonged therapy. Dendrophthoe pentandra (L.) Miq. is a Malaysian mistletoe species that has been used as a traditional treatment for several ailments such as smallpox, ulcers, and cancers. Methods: We developed a resistant cell line (designated as K562R) by long-term co-culture of a BCR/ABL positive CML cell line, K562, with imatinib mesylate. We then investigated the anti-proliferative effects of D. pentandra methanol extract on parental K562 and resistant K562R cells. Trypan blue exclusion assays were performed to determine the IC50 concentration; apoptosis and cell cycle analysis were conducted by flow cytometry. Results: D. pentandra extract had greater anti-proliferative effects towards K562R ($IC50=192{\mu}g/mL$) compared to K562 ($500{\mu}g/mL$) cells. Upon treatment with D. pentandra extract at the IC50. concentration: K562 but not K562R demonstrated increase in apoptosis and cell cycle arrest in the G2/M phase. Conclusion: D. pentandra methanol extract exerts potent anti-proliferative effect on BCR/ABL positive K562 cells.

Mobile Electronic Voting System for Improving of Election Process Student Representatives (학생임원 선출 방법의 개선을 위한 모바일 전자투표 시스템)

  • Oh, Pill-Woo;Shin, Soo-Bum;Kim, Myeong-Ryeol
    • Journal of The Korean Association of Information Education
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    • v.10 no.1
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    • pp.119-127
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    • 2006
  • This paper is designed to search the new alternatives to supplement the inconvenience of the traditional written ballot method which is executed every early semester to select the class board at the school. It is prepared on the based the results of the design and implement of the wired/wireless inter-working mobile electronic voting system where the students can participate in the real-time class board selection and the decision-making utilizing the mobile phones, PDA and PC they commonly have. It is time when we should consider introducing the electronic voting system, to minimize the students' inconvenience and the subsequent missing class, having to wait in the long line in the designated place to select the class board at every election season. This system enables the students to participate wherever they are other than the common place as well. Further, this research will provide the opportunity to think over the new school election culture in line with the age of Ubiquitous, as well as the useful means in the field to promote the active participation of the parents and students in the students' self-administration, decision-making necessary at the schools.

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A Study on Court's Dancing Costume - A Comparison between the Court's Dancing Costume and the People's Clothes - (궁중 무용의상에 관한 연구 - 유럽 일반 복식과의 비교를 중심으로 -)

  • 정옥임;김경희
    • Journal of the Korean Society of Costume
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    • v.52 no.7
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    • pp.123-138
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    • 2002
  • This study as one of a series of systematic studies about dancing costumes will compare the costumes of the 16th. 17th century with their normal style of dress. In brief. this study wants people to know about 16th, 17th century court dancing costumes and to understand the culture in the 16th.17th century. From the comparison between the court's dancing costume and the people's clothes, we could get the conclusion as follows. First, pourpoint. chemis, gown, chausses, etc. that people wore were used for the court's dancing costume. While people Pursued the beauty with the ample silhouette. there were used wrinkles. slits. and tailored oblique line-clothes that gave vitality. Second, silk. linen. brocade. etc. that were used for people's chemises were used for the dancing costume. The good quality and beautiful colors of the dancing costume. however. excelled than of the brilliant court's clothes and the people's clothes. Third. the hair style of dancers were very colorful. For example, there were many kinds of hats and furs, corals, etc. that were attached to them on the most of the long curly hair. In summary, for the court's dancing costume, the same type of clothes was used like the people's clothes. There, however, were differences. The slits, tailored oblique line-clothes and shortened skirt gave the activity. There were many decorations symbolizing the nobles' wealth and Power. Especially the use of the decoration of hair and other stake properties was an effective means for expression. The use of personified masks also contributed to the development of the court dance.

A study on the visual image of men's suit (남성 정장 수트의 시각적 이미지에 관한 연구)

  • Kim, Jeong-Mee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.1
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    • pp.113-127
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    • 2021
  • This study aims to analyze the style of men's suits and extract expressional words for the development of semantic differential scales of visual images according to the change in silhouette. Research methods are literature studies, case studies, and questionnaires. The stimuli were created using CLO 3D, a virtual sewing CAD. This study's results are as follows: 1) The British suit jacket has an angled shoulder line using thin pads, and a slightly higher waistline. The pants of the suit have a high waistline and two pleats, so the thighs of the pants are wide. The American suit jacket has a natural shoulder line, and the waistline is not emphasized. The width of the jacket is relaxed, and the length is long. The pants of the suit have a higher waistline, and the pants are wide as well. The Italian suit jacket has wide shoulders, and the waistline is connected lower with a soft curve, and the length is relatively short. The trousers of the suit are tapered and get narrower as it goes down to the hem. 2) Because it originated in the practices of true bespoke tailoring, traditional British suits have a far more 'fitted' silhouette than the mass-produced styles that became emblematic of the American style. By the late 1950s, a sack-style suit was standard American business attire. This technique gives the sack suit its characteristically 'boxy' silhouette. The "Continental" presented a highly tailored silhouette, with padded shoulders, a slim, tight-fitting chest, and a closely tapered waist. The story was that Italian culture valued aesthetics over all else and thus sought the 'slim' suit style as much as possible. 3) The main expressional words of visual images for men's suits differ greatly depending on the silhouette of suit. The visual images are ranked in the order of 'neat', 'classic', 'educated', 'hard', 'mature', for fitted silhouettes. The words of 'masculine', 'basic', 'comfortable', 'simple', 'mature', 'conservative', 'modern', are ranked for boxy silhouettes. And the words of 'slim', 'young', 'neat', are noted for slim silhouettes.