• Animal cells and systems
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• v.15 no.1
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• pp.37-43
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• 2011

The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight micro satellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These micro satellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.169-190
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• 2006

Researches in livestock are currently actively progressing to improve economically important traits using DNA markers. In cattle, the candidate genes have been selected based on their known functions in the target QTL (quantitative trait locus) region in order to identify QTN (quantitative trait nucleotide) for improving productivities. In this review, molecular genetic studies for the meat related traits, one of the major determinant of market prices, have been fully described. Also recent emerging microarray technique for identifying candidate genes in cattle has been discussed. In case of microarray, cDNA microarrays have been replaced to oligoarrays in order to minimize the experimental errors in cattle. Since the first draft of bovine genome sequences was appeared in the public domain, more markers in relation to the quantitative traits will be discovered in a short period of time and genes affecting difficult-to-measure traits, such as disease resistance, can also be selected for marker assisted selection in near future.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.16 no.2
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• pp.45-53
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• 2002

This paper presents an algorithm for the appropriate parameter selection of a power system stabilizer and power converters in two-area power systems with a series HVDC links. The method for PSS is one of the classical techniques by allocating properly poly-zero positions to fit as closely as desired the ideal phase lead and by changing the gain to produce a necessary damping torque. Proper parameter of power converters are obtained in order to have sufficient speed and stability margin to cope with changing reference values and disturbances based on the Root-locus technique. The small signal and transient stability studies using the PSS and power converters parameters obtained from these methods show that a natural oscillation frequency of the study case system is adequately damped. The simulation used in the paper was performed by the Power System Toolbox software program based on MATLAB.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.6
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• pp.3001-3006
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• 2016

Background: The cancer progression of oral leukoplakia is an important watchpoint in the follow-up observation of the patients. However, potential malignancies of oral leukoplakia cannot be estimated by histopathologic assessment alone. We evaluated genetic abnormalities at the level of copy number variation (CNV) to investigate the risk for developing cancer in oral leukoplakias. Materials and Methods: The current study used 27 oral leukoplakias with histological evidence of dysplasia. The first group (progressing dysplasia) consisted of 7 oral lesions from patients with later progression to cancer at the same site. The other group (non-progressing dysplasia) consisted of 20 lesions from patients with no occurrence of oral cancer and longitudinal follow up (>7 years). We extracted DNA from Formalin-Fixed Paraffin-Embedded (FFPE) samples and examined chromosomal loci and frequencies of CNVs using Taqman copy number assays. Results: CNV frequently occurred at 3p, 9p, and 13q loci in progressing dysplasia. Our results also indicate that CNV at multiple loci-in contrast to single locus occurrences-is characteristic of progressing dysplasia. Conclusions: This study suggests that genetic abnormalities of the true precancer demonstrate the progression risk which cannot be delineated by current histopathologic diagnosis.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2001.11a
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• pp.65-72
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• 2001

Power system stabilizer act efficiently to damp the electromechanical oscillations in interconnected power systems. This paper presents an algorithm for the optimal parameter selection of a power system stabilizer in two-area power systems with a series HVDC link. This method is one of the classical techniques by allocating properly pole-zero positions to fit as closely as desired the ideal phase lead between the voltage reference and the generator electrical power and by changing the gain to produce a necessary damping torque over the matched frequency range. Control of HVDC converter and inverter are used a constant current loop. Proper parameters of PI controllers are obtain based on the Root-locus technique in other to have sufficient speed and stability margin to cope with charging reference values and disturbance. The small signal stability arid transient stability studies using the PSS parameters obtained from this method show that a natural oscillation frequency of the studycase system is adequately damped. Also the simulation results using the HVDC converter and inverter parameters obtained from this proposed method show proper current control characteristics. The simulation used in the paper was performed by the Power System Toolbox software program based on MATLAB.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.1
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• pp.43-50
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• 2016

Quantitative trait locus (QTL) is a particular region of the genome containing one or more genes associated with economically important quantitative traits. This study was conducted to identify QTL regions for body weight and growth traits in purebred Korean native chicken (KNC). F1 samples (n = 595) were genotyped using 127 microsatellite markers and 8 single nucleotide polymorphisms that covered 2,616.1 centi Morgan (cM) of map length for 26 autosomal linkage groups. Body weight traits were measured every 2 weeks from hatch to 20 weeks of age. Weight of half carcass was also collected together with growth rate. A multipoint variance component linkage approach was used to identify QTLs for the body weight traits. Two significant QTLs for growth were identified on chicken chromosome 3 (GGA3) for growth 16 to18 weeks (logarithm of the odds [LOD] = 3.24, Nominal p value = 0.0001) and GGA4 for growth 6 to 8 weeks (LOD = 2.88, Nominal p value = 0.0003). Additionally, one significant QTL and three suggestive QTLs were detected for body weight traits in KNC; significant QTL for body weight at 4 weeks (LOD = 2.52, nominal p value = 0.0007) and suggestive QTL for 8 weeks (LOD = 1.96, Nominal p value = 0.0027) were detected on GGA4; QTLs were also detected for two different body weight traits: body weight at 16 weeks on GGA3 and body weight at 18 weeks on GGA19. Additionally, two suggestive QTLs for carcass weight were detected at 0 and 70 cM on GGA19. In conclusion, the current study identified several significant and suggestive QTLs that affect growth related traits in a unique resource pedigree in purebred KNC. This information will contribute to improving the body weight traits in native chicken breeds, especially for the Asian native chicken breeds.

• 제어로봇시스템학회:학술대회논문집
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• 1999.10a
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• pp.244-247
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• 1999

This paper presents a methodology of the technique for controlling DC motor drive by implementation of 2-quadrant operating mode which can ensure the torque controlling and speed with response time less than 2 seconds at all loading conditions. By implementation of BRM technique, energy is fed with definite values of BRM 256 bits, with different patterns of high accuracy, and fixing scan time at 0.667 ms, the ripple is less than 1%, thus high efficiency can be achieved, from the consequence of the accuracy of energy feeding at low current. The stability of the whole system can be determined from circle criterion by root locus method . The instant reverse direction of rotation can be done by decreasing the energy to the lowest level while motor is running with no load and variable load at the speed about 100-120 rpm and 50-60 rpm.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.4
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• pp.587-596
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• 2001

In the last decade, rapid developments in molecular biotechnology and of genomic tools have enabled the creation of dense linkage maps across whole genomes of human, plant and animals. Successful development and implementation of interval mapping methodologies have allowed detection of the quantitative trait loci (QTL) responsible for economically important traits in experimental and commercial livestock populations. The candidate gene approach can be used in any general population with the availability of a large resource of candidate genes from the human or rodent genomes using comparative maps, and the validated candidate genes can be directly applied to commercial breeds. For the QTL detected from primary genome scans, two incipient fine mapping approaches are applied by generating new recombinants over several generations or utilizing historical recombinants with identity-by-descent (IBD) and linkage disequilibrium (LD) mapping. The high resolution definition of QTL position from fine mapping will allow the more efficient implementation of breeding programs such as marker-assisted selection (MAS) or marker-assisted introgression (MAI), and will provide a route toward cloning the QTL.

• Journal of Power Electronics
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• v.15 no.1
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• pp.288-297
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• 2015

This paper presents a novel approach to diagnose interturn insulation faults in three-phase transformers that operate at different loading conditions. This approach is based on the loci of instantaneous symmetrical components and requires the measurement of three input primary winding currents and voltages to diagnose faults in the transformer. The effect of unbalance supply conditions, load variations, constructional imbalance, and measurement errors when this methodology is used is also investigated. Wing size or length determines the loading on the transformer. Wing travel and area determine the degree of severity of fault. Experimental results are presented for a 400/200 V, 7.5 kVA transformer to validate this method.

• Proceedings of the KIEE Conference
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• 1999.07a
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• pp.220-222
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• 1999

This paper presents a numerical analysis of the eddy current testing with cracked pipe using finite element method (FEM). ${\vec{A}},\;{\phi}-{\vec{A}}$ method is adopted for the formulation of 3-dimensional(3-D) FEM with the brick element. The cracks investigated here are the inner and outer surface of axial symmetry, 90 degree circular one. The algorithm of 3-D numerical analysis is employed for the axisymmetric pipe with the cracks. In order to verify the validity of 3-D numerical analysis, the results are compared with those of 2-D analysis with the same type of the model. The differential impedance is obtained by using energy method and its locus are various 8-shaped curves for each cracks. The ICCG method is used for the calculation of a matrix.